Operum P. Ovidii Nasonis editio nova /

Blanks: v. 1: p. [2], [4] (1st group), p. [1]-[2] (2nd group); v. 3: p. [2] (1st group), p. [1] (2nd group).

P. Ovidii Nasonis Metamorphoseon : Libri XV /

"Index vocabularum omnium": p. [1]-[173] at end.

R.P. Joannis Cabassutii ... Notitia ecclesiastica historiarum conciliorum [et] canonum in vicem collatorum ... ecclesiae rituum ab ipsis Ecclesia christiana incunabulis ...

Mode of access: Internet.

Hand receipt manual covering Basic issued items (BII) and Additional authorization list (AAL) for truck, tractor, 22 1/2-ton, 8x8, M746, national stock no. 2320-00-089-7264.

"October 1983."

Operator's organizational, direct support and general support maintenance manual (including repair parts and special tool list) : chassis, trailer, 3 1/2-ton, 2-wheel, M536 (2330-777-2957), (used with laundry unit, trailer mounted, M532) ...

Includes indexes.

Organizational maintenance repair parts and special tools lists : crane, wheel mounted: 5 ton capacity at 10-foot radius, 3/8 cu. yd; 1 engine diesel driven, 4 x 4 air transportable (Hanson Machinery model H446), FSN 3810-948-0497.

Includes index.

Organizational and direct support, repair parts, and special tools list for drill, pneumatic, drifter, crawler mounted, self propelled, dry type, 220 lb class, minimum 4-1/2 inch bore, power feed, 1-1/2 inch lugged shank chuck w/ 12 each [sic] shank rock drill (Chicago Pneumatic Tool Company model G-900) (NSN 3820-00-930-1917).

"May 1976."

Equipment serviceability criteria for compressor, reciprocating, air, power driven, high pressure, 4 CFM and up, 1000 PSI and up, group I--four wheel MTD, pneumatic tires, GED, Davey mdl P-4, Joy mdls 15HGPS-MSI and 15HGP9-MSI, Joy mdl WK-80 15H1, Joy mdl 415HGP3-MSI, Ingersoll-Rand mdl P4R 15 G-J, Stewart-Warner mdl 12021A ...

"20 June 1973"--P. 1.

Organizational maintenance repair parts and special tools lists for radio sets AN/GRC-50(V)1 (NSN 5820-00-892-3851) ... and AN/GRC-50A(V)11 (NSN 5820-00-136-4966).

Includes index.

Still picture camera set KS-15(1) and camera equipment AN/GFQ-2 : operation and maintenance.

"October 1958."

R.P. Natalis Alexandri ... Historia Ecclesiastica veteris nouique Testamenti ... /

Mode of access: Internet.

C. Iulii Caesaris quae exstant : ex nupera ?viri docti accuratissima recognitione. Accedit nunc vetus interpres Græcus librorum VII De bello Gallico, ex bibliotheca P. Petauii /

En p. [1]-579 de la segunda secuencia: "In C. Iul. Cæsaris Commentarios notæ, adnotationes, commentarii Rhellicani ... ... Nomenclator geographicus præterea duplex; e quibus alter R. Marliani ... " con portadilla propia.

Structure and metal binding studies of the second copper binding domain of the Menkes ATPase

Biological utilisation of copper requires that the metal, in its ionic forms, be meticulously transported, inserted into enzymes and regulatory proteins, and excess be excreted. To understand the trafficking process, it is crucial that the structures of the proteins involved in the varied processes be resolved. To investigate copper binding to a family of structurally related copper-binding proteins, we have characterised the second Menkes N-terminal domain (MNKr2). The structure, determined using H-1 and N-15 heteronuclear NMR, of the reduced form of MNKr2 has revealed two alpha-helices lying over a single beta-sheet and shows that the binding site, a Cys(X)(2)Cys pair, is located on an exposed loop. H-1-N-15 HSQC experiments demonstrate that binding of Cu(I) causes changes that are localised to conserved residues adjacent to the metal binding site. Residues in this area are important to the delivery of copper by the structurally related Cu(I) chaperones. Complementary site-directed mutagenesis of the adjacent residues has been used to probe the structural roles of conserved residues. (C) 2003 Published by Elsevier Inc.

Conditional inactivation of the Men1 gene leads to pancreatic and pituitary tumorigenesis but does not affect normal development of these tissues

Mutations of the MEN1 gene, encoding the tumor suppressor menin, predispose individuals to the cancer syndrome multiple endocrine neoplasia type 1, characterized by the development of tumors of the endocrine pancreas and anterior pituitary and parathyroid glands. We have targeted the murine Men1 gene by using Cre recombinase-loxP technology to develop both total and tissue-specific knockouts of the gene. Conditional homozygous inactivation of the Men1 gene in the pituitary gland and endocrine pancreas bypasses the embryonic lethality associated with a constitutional Men1(-/-) genotype and leads to beta-cell hyperplasia in less than 4 months and insulinomas and prolactinomas starting at 9 months. The pituitary gland and pancreas develop normally in the conditional absence of menin, but loss of this transcriptional cofactor is sufficient to cause beta-cell hyperplasia in some islets; however, such loss is not sufficient to initiate pituitary gland tumorigenesis, suggesting that additional genetic events are necessary for the latter.

Identification and cloning of the gene encoding BmpC: an outer-membrane lipoprotein associated with Brachyspira pilosicoli membrane vesicles

The intestinal spirochaete Brachyspira pilosicoli causes colitis in a wide variety of host species. Little is known about the structure or protein constituents of the B. pilosicoli outer membrane (OM). To identify surface-exposed proteins in this species, membrane vesicles were isolated from B. pilosicoli strain 95-1000 cells by osmotic lysis in dH(2)O followed by isopycnic centrifugation in sucrose density gradients. The membrane vesicles were separated into a high-density fraction (HDMV; p = 1.18 g CM-3) and a low-density fraction (LDMV; rho=1.12 g cm(-3)). Both fractions were free of flagella and soluble protein contamination. LDMV contained predominantly OM markers (lipo-oligosaccharide and a 29 kDa B. pilosicoli OM protein) and was used as a source of antigens to produce mAbs. Five B. pilosicoli-specific mAbs reacting with proteins with molecular masses of 23, 24, 35, 61 and 79 kDa were characterized. The 23 kDa protein was only partially soluble in Triton X-114, whereas the 24 and 35 kDa proteins were enriched in the detergent phase, implying that they were integral membrane proteins or lipoproteins. All three proteins were localized to the B. pilosicoli OM by immunogold labelling using specific mAbs. The gene encoding the abundant, surface-exposed 23 kDa protein was identified by screening a B. pilosicoli 95-1000 genome library with the mAb and was expressed in Escherichia coli. Sequence analysis showed that it encoded a unique lipoprotein, designated BmpC. Recombinant BmpC partitioned predominantly in the OM fraction of E. coli strain SOLR. The mAb to BmpC was used to screen a collection of 13 genetically heterogeneous strains of B. pilosicoli isolated from five different host species. Interestingly, only strain 95-1000 was reactive with the mAb, indicating that either the surface-exposed epitope on BmpC is variable between strains or that the protein is restricted in its distribution within B. pilosicoli.