Rapid quantitative detection of Yersinia pestis by lateral-flow immunoassay and up-converting phosphor technology-based biosensor

Up-converting phosphor technology (UPT)-based lateral-flow immunoassay has been developed for quantitative detection of Yersinia pestis rapidly and specifically. In this assay, 400 nm up-converting phosphor particles were used as the reporter. A sandwich immumoassay was employed by using a polyclonal antibody against F1 antigen of Y. pestis immobilized on the nitrocellulose membrane and the same antibody conjugated to the UPT particles. The signal detection of the strips was performed by the UPT-based biosensor that could provide a 980 nm IR laser to excite the phosphor particles, then collect the visible luminescence emitted by the UPT particles and finally convert it to the voltage as a signal. V-T and V-c stand for the multiplied voltage units for the test and the control line, respectively, and the ratio V-T/V-C is directly proportional to the number of Y pestis in a sample. We observed a good linearity between the ratio and log CFU/ml of Y pestis above the detection limit, which was approximately 10(4) CFU/mI. The precision of the intra- and inter-assay was below 15% (coefficient of variation, CV). Cross-reactivity with related Gram-negative enteric bacteria was not found. The UPT-LF immunoassay system presented here takes less than 30 min to perform from the sample treatment to the data analysis. The current paper includes only preliminary data concerning the biomedical aspects of the assay, but is more concentrated on the technical details of establishing a rapid manual assay using a state-of-the-art label chemistry. (c) 2006 Elsevier B.V. All rights reserved.

Report upon the commercial fisheries in the Lancashire River Authority Area and their effect upon rod angling and spawning stock in the rivers and particularly the River Lune

The stock of salmon in any river is largely dependent upon the escapement of sufficient fish to ensure that an adequate quantity of ova is deposited and full use is made of all suitable spawning areas. At the present time, no accurate information is available on the numbers of fish entering rivers. Therefore, catch returns provide valuable information on fish stocks. This report summarises the catch returns for salmon in the Lancashire River Authority in the North of England for the years 1960-1964. Rivers included are the Lune, Ribble, Kent, Leven and Duddon.

Salmon and freshwater fisheries in the Solway Firth. Report of the Working Party of North West Water Authority and the Annan District Salmon Fishery Board.

The Workinq Party of North West Water Authority and the Annan District Salmon Fishery Board's aims were to identify the current problems relating to the salmon and freshwater fisheries in the Solway Firth and its tributaries (embracing the catchments of the Annan, Kirtle Water, Sark, Esk, Eden, Wampool and Waver - and possibly also the Lochar Water and the Nith) to produce agreed outline solutions, and to consider the financial aspects of the future administration of these fisheries.

Salmon stocking by the Lancashire River Authority: investigations into the requirements and methods best suited to the area

All species of fish are able to propagate and maintain their numbers provided that no adverse influence occurs to change the compatible environment, the salmon is no exception. Propagation of fish by artificial means has long been a subject of discussion amongst fishery workers and views have been expressed (both favourable and unfavourable) on the merits of the various methods employed. In an attempt to discover whether artificial propagation was necessary and also to find the best methods of propagation to adopt in the various rivers, a phased programme of investigation into natural spawning efficiency and the results obtained by various methods of artificial propagation was started in the Lancashire River Board area during 1957. The object being to seek information on: (1) The survival of ova from natural spawnings to the eyed and alevin stages. (2) The population density of feeding fry (from natural spawnings) at various intervals of development. (3) The viability of green ova and eyed ova- when planted artificially. (4a) The survival to 0+ parr from implants of eyed ova unfed fry and fed fry. (4b) Populations per unit area of 0+ parr from various planting densities of eyed ova, unfed fry and fed fry. Sampling stations were selected on the Rivers Ribble, Lune and Wyre watersheds for the purpose of marking and examination of natural salmon redds.

Report on eight weeks at the Fisheries Department of the Lancashire River Authority [including a Leeds / Liverpool Canal survey]

This report looks at the interests of the Fisheries Department for the Lancashire River Board, focusing on the Liverpool and Leeds Canal Survey. It looks at finding parasites in fish from an electrofishing survey (roach and perch). This was to contribute towards the main survey with a possible view to suggesting whether or not the parasites were one reason for the poor fish population. Methods of finding parasites are given, and results of what parasites that were found in the fish are discussed.

Effects of rapid decompression and exposure to bright light on visual function in black rockfish (Sebastes melanops) and Pacific halibut (Hippoglossus stenolepis)

Demersal fishes hauled up from depth experience rapid decompression. In physoclists, this can cause overexpansion of the swim bladder and resultant injuries to multiple organs (barotrauma), including severe exophthalmia (“pop-eye”). Before release, fishes can also be subjected to asphyxia and exposure to direct sunlight. Little is known, however, about possible sensory deficits resulting from the events accompanying capture. To address this issue, electroretinography was used to measure the changes in retinal light sensitivity, flicker fusion frequency, and spectral sensitivity in black rockfish (Sebastes melanops) subjected to rapid decompression (from 4 atmospheres absolute [ATA] to 1 ATA) and Pacific halibut (Hippoglossus stenolepis) exposed to 15 minutes of simulated sunlight. Rapid decompression had no measurable influence on retinal function in black rockfish. In contrast, exposure to bright light significantly reduced retinal light sensitivity of Pacific halibut, predominately by affecting the photopigment which absorbs the green wavelengths of light (≈520–580 nm) most strongly. This detriment is likely to have severe consequences for postrelease foraging success in green-wavelength-dominated coastal waters. The visual system of Pacific halibut has characteristics typical of species adapted to low light environments, and these characteristics may underlie their vulnerability to injury from exposure to bright light.