Composição florística e estrutura fitossociológica de dois fragmentos de floresta de restinga no Município de Bertioga, SP, Brasil

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Horizontal and vertical tree community structure in a lowland atlantic rain forest, southeastern Brazil

A estrutura horizontal e vertical do componente arbóreo foi investigada em um trecho de Floresta Atlântica baixo-montana através de um levantamento fitossociológico em dois blocos amostrais de 0,99 ha cada no Parque Estadual Intervales. Todos os indivíduos com DAP > 5 cm foram registrados. Foram amostrados 3.078 indivíduos distribuídos em 172 espécies. O índice de diversidade de Shannon foi de H' = 3,85 nat.ind.-1. A família Myrtaceae se destacou tanto em número de espécies (38) quanto em número de indivíduos (745) no levantamento. Euterpe edulis Mart. teve o maior valor de importância (33,98%), abrangendo 21,8% do total de indivíduos registrados. O índice de similaridade quantitativo foi maior do que o qualitativo, mostrando pouca variação estrutural entre os blocos amostrais, mas a grande quantidade de espécies pouco abundantes, resultou em pronunciadas diferenças florísticas entre eles. Uma análise de correspondência retificada (DCA) gerou três estratos verticais arbitrários. O estrato A (> 26 m) teve a menor densidade e foi bem representado pelas espécies Sloanea guianensis (Aubl.) Benth. e Virola bicuhyba (Schott. ex A.DC.) Warb. O estrato B (8 m < h < 26 m) mostrou a maior riqueza e diversidade florística, e o estrato C (< 8 m) a maior densidade. Euterpe edulis, Guapira opposita (Vell.) Reitz, Garcinia gardneriana (Planch. & Triana) Zappi e Eugenia mosenii (Kausel) Sobral foram bem representadas nos estratos B e C da floresta. A existência de estratos verticais em florestas tropicais é discutida, recomendando-se o uso da DCA para estudos da estratificação vertical em outras florestas tropicais.

Florística e fitossociologia em parcelas permanentes da Mata Atlântica do sudeste do Brasil ao longo de um gradiente altitudinal

This paper summarizes floristic and phytossociology data of 11, out of 14 plots of 1 ha, allocated along an altitudinal gradient in the Serra do Mar, Silo Paulo, Brazil. The study was conducted at Serra do Mar State Park and the plots start at the sea level (10 m - plot of Restinga Forest that occurs at Praia da Fazenda, Picinguaba, municipality of Ubatuba) up to 1100 m above sea level (the Montane Ombrophilous Dense occurs alongside the Itamambuca Trail, municipality of Silo Luis do Paraitinga). The Restinga Forest occurs in Pleistocenic Coastal Plain where the soil is classified as a sandy Quartzipsamment (Quartzenic Neosol), while along the slopes of the Serra do Mar, the Ombrophylus Dense Forest grows on the top of a pre-Cambrian crystalline basement with granitic rocks, where the soil is a sandy-loam Dystrophic Inceptisol (Cambisol/Latosol). In all 14 plots soils are acidic (pH 3 - 4), chemically poor, with high dilution of nutrients and high saturation of aluminum. In the Restinga and at the foot of the slope the climate is Tropical/Subtropical Humid (Af/Cfa), with no dry season, an average annual rainfall over 2,200 mm and an average annual temperature of 22 degrees C. Towards the top of the Serra do Mar there is a gradual cooling along the slope, but there is no reduction in rainfall, so at 1,100 m above sea level the climate is classified as Humid Subtropical (Cfa/Cfb), with no dry season and an average annual temperature of 17 degrees C. It is important to remark that, almost daily, from 400 m above sea level up to the top of slopes the mountains are covered by a dense fog. In the 14 plots 21,733 individuals with DBH >= 4.8 cm, including trees, palms and ferns, were marked, measured and sampled. The average number of individuals sampled in each plot was 1264 ind.ha(-1)(+/- 218 SE 95%). Within the parameters considered trees prevailed (71% in the Montane ODF to 90% in the Restinga Forest), followed by palms (10% in the RF and 25% in the Montane Ombrophilous Dense Forest/ODF) and ferns (0% % in the RF and 4% in the Montane ODF). Regarding these proportions the Exploited Lowlands ODF differs from the others with only 1.8% of palm trees and striking 10% of ferns. The forest canopy is irregular with heights ranging from 7 to 9 m, rarely emergent trees reach 18 m, and due to this irregularity of the canopy the amount of light that gets through sets conditions for the development of hundreds of epiphytic species. Aside from Montana ODF, where the number of dead trees was more than 5% of individuals sampled, in the other phytophysiognomies this value was below 2.5%. In the 11 plots where the floristic study was conducted we found 562 species in 195 genera and 68 families. Only seven species - Euterpe edulis Mart. (Arecaceae), Calyptranthes lucida Mart. ex DC. and Marlierea tomentosa Cambess (both Myrtaceae), Guapira opposita (Veil.) Reitz (Nyctaginaceae), Cupania oblongifolia Mart. (Sapindaceae), Cecropia glaziovii Snethl. and Coussapoa microcarpa (Schott) Rizzini (both Urticaceae) - occurred from Restinga to Montane ODF, while 12 other species did not occur only in the Restinga Forest. Families with the greatest number of species are Myrtaceae (133 spp), Fabaceae (47 spp), Rubiaceae (49) and Lauraceae (49) throughout the gradient and Monimiaceae (21) specifically in portions Montane ODF. Only in the F plot, where logging has occurred between 1950 and 1985, the abundance of palm trees has been replaced by Cyatheaceae. The study shows a peak of diversity and richness, Shannon-Weiner index (H') ranging from 3.96 to 4.48 nats.ind(-1), in the intermediate altitudes (300 to 400 m) along the slope. Several explanations for this result are raised here, including the fact that these elevations are within the limits expansions and retractions of the different phytophysiognomies of the Atlantic ODF due to climate fluctuations during the Pleistocene. The results presented in this paper demonstrate the extraordinary richness of tree species of the Atlantic Rainforest from the northeastern coast of the State of São Paulo, reinforcing the importance of its conservation throughout the altitudinal gradient. The richness of this forest justifies a long term commitment to study its dynamics and functioning through permanent plots, and monitor the impacts of climate change in this vegetation.

Florística e fitossociologia do componente arbóreo da transição Floresta Ombrófila Densa das Terras Baixas - Floresta Ombrófila Densa Submontana do Núcleo Picinguaba/PESM, Ubatuba, sudeste do Brasil

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Negative chronotropic response to adenosine receptor stimulation in rat right atria after run training

The aim of the present study was to evaluate the potency and maximal responses (E-max) to the adenosine receptor agonists N-6-cyclopentyladenosine (CPA), N-ethylcarboxamidoadenosine (NECA) and N-6-(3-iodobenzyl)-5'-N-methylcarbaxamidoadenosine (IB-MECA) in right atria from trained rats. We also investigated the interaction between the training bradycardia and the sensitivity of the chronotropic response mediated by adenosine receptor stimulation.2. Animals were submitted to run training for 60 min, 5 days a week, over a period of 8 weeks. Mean blood pressure and heart rate were measured in conscious animals. Right atria were isolated and concentration-response curves to CPA, NECA and IB-MECA were obtained.3. A reduction in heart rate was found in trained rats, indicating that the training programme was successful in inducing physical conditioning. The three adenosine receptor agonists induced a concentration-dependent negative chronotropic response. The rank order of potency and E-max for the three adenosine receptor agonists was CPA>NECA>IB-MECA.4. Dynamic exercise for 8 weeks did not alter the E a, for CPA, NECA and IB-MECA. Similarly, the potencies of CPA and NECA were not affected by run training, whereas the potency of IB-MECA was reduced (6.10+/-0.09 vs 5.66+/-0.10 for sedentary and trained groups, respectively).5. In conclusion, run training for 8 weeks induced a desensitization of the chronotropic response to IB-MECA without changing the potency of CPA and NECA. These findings exclude the participation of adenosine receptors in the training bradycardia.

Breeding activity patterns, reproductive modes, and habitat use by anurans (Amphibia) in a seasonal environment in the Pantanal, Brazil

Annual patterns of breeding activity, reproductive modes, and habitat use are described for a frog community in a seasonal environment, in the southern Pantanal, Mato Grosso do Sul, Brazil. Data were collected monthly between January 1995 and December 1998. A total of 24 species from four families; Bufonidae (3 species), Hylidae (10 species), Leptodactylidae (9 species), and Microhylidae (2 species) were registered. Three reproductive activity patterns are recognized among these species: continuous, explosive, and prolonged; 50% of the species were explosive breeders. Seasonal pattern of reproduction was verified for three analyzed years (1995-1997) most species reproduced during the rainy season (Nov-Jan). The reproduction was aseasonal in 1998; unexpected rains in the dry season lead to an unusual breeding activity. Five reproductive modes were noted - 62.5% of the species have the generalized aquatic mode, and 33.3% deposit eggs embedded in foam nests. Many species used the same sites for reproduction, although temporal partitioning and calling site segregation was observed. The occurrence of many species that exhibit explosive breeding early in the rainy season is common in seasonal and open environments with variable and unpredictable rainfall, as is the case in the Pantanal.

Caracterização e estudo da corrosão do amálgama dentário Dispersalloy por meio das técnicas de polarização potenciodinâmica e espectroscopia de impedância

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Production of defects in ZBLAN, ZBLAN : Tm3+ and ZBLAN : Cr3+ glasses by ultra-short pulses laser interaction

In this work we have studied pure and thulium- and chromium-doped ZBLAN glasses irradiated by ultra-short laser pulses. A Ti:sapphire CPA system was used, producing a 500 Hz train of pulses, centered at 830 nm, with 375 mu J of energy and 50 fs of duration (FWHM). The beam was focused by a 20 Him lens, producing a converging beam with a waist of 12 pin. The absorption spectra before and after laser irradiation were obtained showing production of color centers in pure, thulium-doped and chromium-doped ZBLAN glasses. A damage threshold of 9.56 TW/cm(2) was determined for ZBLAN. (C) 2007 Elsevier Ltd. All rights reserved.

The invertebrate colonization during decomposition of Eichhornia azurea kunth in a lateral lake in the mouth zone of Paranapanema River into Jurumirim Reservoir (São Paulo, Brazil)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Spatial and temporal distribution of the zoobenthos community during the filling up period of Porto Primavera Reservoir (Paraná River, Brazil)

Este estudo é parte do monitoramento limnológico empreendido pela Companhia Energética do Estado de São Paulo (CESP) durante o processo de enchimento do reservatório de Porto Primavera (Usina Hidrelétrica Engenheiro Sérgio Motta). Este reservatório, localizado no alto rio Paraná, entre os Estados de São Paulo e Mato Grosso, é o quarto maior do país. A primeira etapa de enchimento do lago começou em dezembro de 1998 e a segunda em março de 2001. Amostras para a análise da comunidade bentônica e das características sedimentológicas foram coletadas trimestralmente entre agosto de 1999 e novembro de 2001 e também em agosto de 2002 (11 campanhas). As coletas foram feitas em 13 estações de amostragem distribuídas no reservatório e em uma localizada a jusante da barragem. 128 táxons de invertebrados foram encontrados, sendo Mollusca, Annelida, Insecta e Nematoda os grupos dominantes durante praticamente todos os meses analisados. A classe Insecta foi a melhor representada, com 9 diferentes ordens, dentro das quais os Diptera contribuíram com a ocorrência de 63 táxons. A espécie exótica de bivalve Corbicula fluminea foi registrada em todas as estações de amostragem mostrando sua grande capacidade para colonizar novos habitats em regiões neotropicais. Variações consideráveis na densidade da fauna foram observadas para os diferentes períodos e locais analisados. A densidade máxima (média de 7812 ind.m-2) foi registrada no centro do reservatório enquanto que as densidades mínimas foram registradas na zona lacustre próxima à barragem (média de 9 ind.m-2). A maior riqueza de espécies por local/período (24 táxons) foi encontrada no trecho superior do reservatório (trecho fluvial). A diversidade máxima foi observada nas zonas superior e central do reservatório, com valores de 3.82 e 3.86 (bits.ind-1) no início (agosto/1999) e final (agosto/2002) do processo de enchimento, respectivamente. Não foi encontrado um padrão de distribuição dos grupos faunísticos que pudesse estar associado com a textura granulométrica dos diferentes locais amostrados. Por outro lado, constatou-se a diminuição, ou mesmo a não ocorrência de organismos, nas estações com elevada concentração de matéria orgânica (>40%) em baixo estado de degradação (grandes detritos vegetais). Tal fato pode estar relacionado com a falta de depósitos de sedimentos, dificultando a fixação de organismos da fauna bentônica, bem como com condições químicas mais redutoras em função da intensidade dos processos de decomposição da fitomassa inundada.

Survival of Ucides cordatus (Decapoda: Ocypodidae) megalopae during transport under different conditions of density and duration

Target areas for Ucides cordatus (Linnaeus, 1763) restocking programs are often located far from the laboratory where larval rearing is developed. During translocation, the larvae are submitted to highly stressful conditions due to handling, packing, and transport activities. The aim of the present study was to assess the mortality rates of U. cordatus megalopae caused by different transportation procedures. Megalopae at loading densities of 50, 150, and 300 ind.L-1 were packed in double polyethylene 12 x 25 cm plastic bags with 200 ml of marine water at salinity 30. The bags were filled with oxygen at a proportion of 1:2 parts of water and sealed tightly. The trepidations during transport were simulated by the use of a shaker device (800 vibrations/minute) over periods of three and six hours inside a dark container. The survivorship rates of larvae after simulation were compared to those obtained in control groups, which consisted of plastic vials with megalopae at a loading density of 50 ind.L-1 maintained at rest. Immediately after the two transport simulations, there was no significant difference in survivorship between the treatments and the control. However, 24 hours after simulation some of the tested densities resulted in significantly lower survivorships. The results demonstrated that U. cordatus megalopae can tolerate six hours of shaking during transportation, at high densities with minimal mortality.

Sucessão da comunidade de algas perifíticas em reservatório oligotrófico tropical (São Paulo, Brasil): comparação entre período seco e chuvoso

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Efeito da densidade populacional sobre as curvas de crescimento de Macrobrachium rosenbergii (De Man) em cultivo semi-intensivo (Crustacea, Palaemonidae)

In this article the length growth curves of Macrobrachium rosenbergii (De Man, 1879) held in different population densities were analysed. Postlarvae prawns were stocked into five 0,02 ha earthen ponds at densities of 4, 8, 12, 16 and 20 ind./m² during six months. Forthnightly, the mean total length of animals from each pond were obtained by means of random samples. For each population the length growth curves and the instantaneous growth rate expressions were determined. The growth rate and the asymptotic maximum length decreased with the stocking density raised. This effect is greater when the density ranges in low levels. The growth decrease can be due to intraspecific competition for life resources and to negative interactions between individuals, as aggressive or social behavior, that increase with the increase prawns density.

Abundance, habitat use and diet of Callicebus nigrifrons Spix (Primates, Pitheciidae) in Cantareira State Park, São Paulo, Brazil

Entre abril de 2005 e maio de 2006, através de censos seguindo os pressupostos da metodologia de transecção linear, foram estimadas a densidade populacional e abundância de Callicebus nigrifrons Spix, 1823 (Pitheciidae) no Parque Estadual da Cantareira, Estado de São Paulo, Sudeste do Brasil (23°23'42S, 46°35'27W). Com um esforço amostral de 275,8 km de censos, os sauás foram a segunda espécie de primata mais abundante, apresentando um índice de abundância de 1,4 grupos para cada 10 km percorridos e uma estimativa de densidade de 12,21 ind./km² (variando de 8,45 a 17,63 ind./km²). A coleta de dados auxiliares durante os censos possibilitou a verificação da dieta e uso do hábitat pelos grupos de Callicebus, e os resultados evidenciaram uma relativa adaptabilidade à ambientes perturbados.

Evaluation of a novel kit (TF-test) for the diagnosis of intestinal parasitic infections

Intestinal parasitic infections are currently a source of concern for Public Health agencies in developing and developed countries. Since three ovum-and-parasite stool examinations have been demonstrated to provide sensitive results, we designed a practical and economical kit (TF-Test) that is now commercially available (Immunoassay Com. Ind. Ltda., S (a) over tildeo Paulo, Brazil). This kit allows the separate collection of three fecal specimens into a preservative solution. The specimens are then pooled, double-filtered, and concentrated by a single rapid centrifugation process. The TF-Test was evaluated in four different laboratories in a study using 1,102 outpatients and individuals living in an endemic area for enteroparasitosis. The overall sensitivity found using the TF-Test (86.2-97.8%) was significantly higher (P<0.01) than the sensitivity of conventional techniques such as the Coprotest (NIL Comercio Exterior Ltda, São Paulo, Brazil) and the combination of Lutz/Hoffman, Faust, and Rugai techniques (De Carli, Diagnostico Laboratorial das Parasitoses Humanas. Metodos e Tecnicas, 1994), which ranged from 48.3% to 75.9%. When the above combined three specimen technique was repeated with three specimens collected on different days, its sensitivity became similar (P > 0.01) to that of the TF-Test. The kappa index values of agreement for the TF-Test were consistent (P < 0.01), being higher and ranking in a better position than conventional techniques. The high sensitivity, cost/benefit ratio, and practical aspects demonstrate that the TF-Test is suitable for individual diagnosis, epidemiological inquiries, or evaluation of chemotherapy in treated communities. (C) 2004 Wiley-Liss, Inc.