Manipulation of the Quorum Sensing Signal AI-2 Affects the Antibiotic-Treated Gut Microbiota

The mammalian gut microbiota harbors a diverse ecosystem where hundreds of bacterial species interact with each other and their host. Given that bacteria use signals to communicate and regulate group behaviors (quorum sensing), we asked whether such communication between different commensal species can influence the interactions occurring in this environment. We engineered the enteric bacterium, Escherichia coli, to manipulate the levels of the interspecies quorum sensing signal, autoinducer-2 (AI-2), in the mouse intestine and investigated the effect upon antibiotic-induced gut microbiota dysbiosis. E. coli that increased intestinal AI-2 levels altered the composition of the antibiotic-treated gut microbiota, favoring the expansion of the Firmicutes phylum. This significantly increased the Firmicutes/Bacteroidetes ratio, to oppose the strong effect of the antibiotic, which had almost cleared the Firmicutes. This demonstrates that AI-2 levels influence the abundance of the major phyla of the gut microbiota, the balance of which is known to influence human health.

Gut filling, gut content and fatty acid composition of demersal fish species sampled during POLARSTERN cruise ANT-XXIV/2

The gut contents and fatty acid composition of 49 fish belonging to five Antarctic demersal families (Nototheniidae, Macrouridae, Channichtyidae, Bathydraconidae and Artedidraconidae) sampled at two stations at the Southern Ocean shelf and deep sea (600 and 2150 m) were analysed in order to identify their main food resource by linking trophic biomarkers with the dietary items found in the fish guts. Main food items of most fish analysed were amphipod crustaceans (e.g. in 63% of Trematomus bernachii guts) and polychaetes (e.g. in 80% of Bathydraco sp. guts), but other food items including fish, other crustaceans and gastropods were also ingested. The most prominent fatty acids found were 20:5(n-3), 16:0, 22:6(n-3) and 18:1(n-9). The results of gut content and fatty acid analyses indicate that all fish except the Channichthyidae share similar food resources irrespective of their depth distribution, i.e. benthic amphipods and polychaetes. A difference of the dietary spectrum can be observed with ontogenetic phases rather than between species, as high values of typical calanoid copepod marker fatty acids as 22:1(n-11) indicate that younger (smaller) specimens include more zooplankton in their diet.

Gut content of holothurians from the NE Atlantic

This paper describes inter-specific differences in the distribution of sediment in the gut compartments and in the enzyme and bacterial profiles along the gut of abyssal holothurian species - Oneirophanta mutabilis, Psychropotes longicauda and Pseudostichopus villosus sampled from a eutrophic site in the NE Atlantic at different times of the year. Proportions of sediments, relative to total gut contents, in the pharynx, oesophagus, anterior and posterior intestine differed significantly in all the inter-species comparisons, but not between inter-seasonal comparisons. Significant differences were also found between the relative proportions of sediments in both the rectum and cloaca of Psychropotes longicauda and Oneirophanta mutabilis. Nineteen enzymes were identified in either gut-tissue or gut-content samples of the holothurians studied. Concentrations of the enzymes in gut tissues and their contents were highly correlated. Greater concentrations of the enzymes were found in the gut tissues suggesting that they are the main source of the enzymes. The suites of enzymes recorded were broadly similar in each of the species sampled collected regardless of the time of the year, and they were similar to those described previously for shallow-water holothurians. Significant inter-specific differences in the gut tissue concentrations of some of the glycosidases suggest dietary differences. For example, Psychropotes longicauda and Pseudostichopus villosus contain higher levels of chitobiase than Oneirophanta mutabilis. There were no seasonal changes in bacterial activity profiles along the guts of O. mutabilis and Pseudostichopus villosus. In both these species bacterial activity and abundance declined between the pharynx/oesophagus and anterior intestine, but then increased along the gut and became greatest in the rectum/cloaca. Although the data sets were more limited for Psychropotes longicauda, bacterial activity increased from the anterior to the posterior intestine but then declined slightly to the rectum/cloaca. These changes in bacterial activity and densities probably reflect changes in the microbial environment along the guts of abyssal holothurians. Such changes suggest that there is potential for microbial breakdown of a broader range of substrates than could be otherwise be achieved by the holothurian itself. However, the present study found no evidence for sedimentary (microbial) sources of hydrolytic enzymes.

Relative abundances of planktonic foraminifera from surface sediments of the Drake Passage, Southern Ocean (Table 1)

Based on a qualitative and quantitative evaluation of Recent sediments samples (top 3 cm of cores as well as Petersen grab samples) from the Drake Passage, between South America and Antarctica, the distribution of planktonic foraminifera and their relation to oceanographic conditions was investigated. The Antarctic Convergence - the northern limit of the cold Antarctic Surface Water - is shown to be of major importance in controlling the distributional pattern of planktonic species as well as their total numbers. South of the convergence, Globigerina pachyderma is usually the only species found in the sediment. It occurs with abundances not greater than 6000 per gram dry sediment, and at most stations less than 100 specimens per gram of dry sediment were recovered. At a number of deep-sea stations below 3700 m depth approx. no planktonic foraminifera were found at all. It is most probable, that at least some of these stations are located below the limit of CaCO3 dissolution. North of the Antarctic Convergence planktonic foraminiferal numbers are much higher and range from 1800 to 120000 per gram of dry sediment. Eight species are the major constituents of the population: Globigerina pachyderma, Globigerina bulloides, Globogerina quinqueloba, Globigerina inflata, Globorotalia truncatolinoides, Globorotalia scitula, Globigerinita glutinata and Globigerinita uvula. The widespread occurrence of Globorotalia truncatulinoides, which in the northern hemisphere is usually a subtropical form, is especially noteworthy. Another Globigerina, morphologically similar to G. pachyderma, has been recognized frequently north of the Antarctic Convergence. Globigerina megastoma which has its type area in the Drake Passage, has been found only rarely. Orbulina universa occurs in samples from the areas of higher water temperature around the South American Continent. Globigerina pachyderma is predominantly sinistrally coiled throughout the area investigated, but a slight increase in the percentage of dextrally coiled specimens may be noticed with increasing water temperature, i.e. from south to north.

Golden Gate bridge, California tolls on government traffic : hearings ... 78th Congress, 1st session on H.R. 877, a bill to authorize the charging of tolls for the passage or transit of government traffic over the Golden Gate bridge.

Hearing held March 9, 1943.

Use of safety restraints by law enforcement officers following safety belt training and passage of a state-wide belt law. Final report.

National Highway Traffic Safety Administration, Washington, D.C.