Efeito do exercício físico sobre a prenhez de ratas com hipertensão arterial crônica

Pós-graduação em Ginecologia, Obstetrícia e Mastologia - FMB

Participação hepática e efeito glicocorticoide na resposta inflamatória aguda induzida por Aeromonas hydrophila em tilápia do Nilo, intoxicada por CCl4

Pós-graduação em Medicina Veterinária - FCAV

Estudo imuno-histoquímico da co-expressão de citoqueratinas, vimentina e actina em neoplasias de glândula salivar

Human salivary gland tumors originated from intercalated ducts present a broad range of histologic and cytologic patterns, mainly due to the presence of myoepithelial cells. The aim of this study is to verify the differentiation grade of neoplastic cells and a possible relation between myoepithelial cell differentiation and the presence of luminal secretory contents. The expression of vimentin and cytokeratin (CK) intermediate filaments, actin myofilament and epithelial membrane antigen (EMA) was investigated by double labeling immunocytochemical technique, in thirty salivary gland neoplasms: 5 pleomorphic adenomas, 5 myoepitheliomas, 3 basal cell adenomas, 7 adenoid cystic carcinomas (ACC) and 10 polimorphous low grade adenocarcinomas (PLGA). Tumors with intercalated duct differentiation (pleomorphic adenomas, basal cell adenomas and ACC) express CKs 7, 8, 18 and 19 in the luminal cells and coexpress eventually CK14 with these CKs. Some luminal cells stained with anti-EMA antibody, mainly where a secretory content in the lumen was observed. Outer ductal cells and other myoepithelial-like cells express vimentin, sometimes coexpressing actin and/or CK14 with vimentin. Plasmacytoid cells in myoepitheliomas and pleomorphic adenomas express vimentin and rarely CKs 7, 8, 18 and 19, sometimes coexpressing these CKs with CK14 but they are negative for the remaining antigens. Tumors without intercalated duct differentiation (solid basal cell adenoma and PLGA) express vimentin and CKs 7, 8, 14 and 18, sometimes coexpressing CKs 8 and 18 with CK14. In conclusion, in tumors with intercalated duct differentiation, myoepithelial cells express vimentin and sometimes coexpress actin and/or CK14 with vimentin, never coexpressing other CKs with vimentin. CK14 and actin are independently expressed by myoepithelial cells, so their expression is probably induced by different stimulus. However, the secretory function of luminal cells, visualized by EMA staining, ....

Growth in weight and of some tissues in the bullfrog: fitting nonlinear models during the fattening phase

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Desenvolvimento de nanopartículas de quitosana para adminstração nasal de zidovudina

Zidovudine (AZT) is the drug most commonly used in AIDS treatment, isolated or in combination with other antiretroviral agents, but it has certain limitations due to its therapeutic dose-dependent haematological toxicity. In addition, it has low oral bioavailability, since it undergoes pre-systemic metabolism. The nasal route has been used as an alternative route for drug administration, because it can promote its direct absorption to blood circulation, avoiding hepatic metabolism. However, this route presents as a factor limiting the mucociliary clearance mechanisms that remove quickly the formulation of the nasal cavity. To prolong the residence time of formulations, in this direction, has been proposed the development of mucoadhesive systems. Among the various existing systems, the use of chitosan (QS), as mucoadhesive polymer, has been widely exploited in the preparation of nanoparticles (NPs). The objective of this study was to develop and characterize QS’s NPs for intranasal administration of AZT. For both NPs have been developed by ionic crosslinking of QS with sodium tripolyphosphate (TPP). These NPs were characterized by studies of particle size distribution, zeta potential, morphology, mucoadhesion tests, assessing the ability of encapsulation of the drug and permeation profile of AZT. The evaluation of AZT in the NPs was determined by UV-Vis spectroscopy. Mucoadhesion measures were made using a texture analyzer, using a mucin disk and porcine mucous membrane , and permeation assay were conducted using porcine nasal mucous membrane adapted to the Franz cell. These results suggest that the systems in hand have great potential for nasal AZT administration

Contribuição da utrassonografia para o diagnóstico do shunt portossistêmico em câes e gatos

Portosystemic shunt, an hepatobiliary anomaly, much common in dogs and less frequent in cats, can be detected by several diagnostic methods. Among them, the one which stands out is ultrasonography due to its numerous advantages: noninvasive, quick, accessible, relatively low cost and provides useful information relating to the other systems, apart from being a highly sensitive method. Portosystemic shunt diagnosis using ultrasonography becomes highly sensitive when associated with B-mode, color Doppler and pulsed Doppler. A bibliographic survey about portosystemic shunt ultrasonographic diagnosis was carried out, and the main ultrasonographic signals were: decreased hepatic size, difficulty in blood vessels visualization, presence of an anomalous vessel, tortuous with turbulent flow and, finally, increased portal blood flow velocity near the shunting vessel

Avaliação comparativa da segurança hepática e cardíaca do potencial fármaco antichagásico hidroximetilnitrofural (NFOH) com nitrofurazona (NF) em coelhos

A tripanossomíase americana – Doença de Chagas – é uma parasitose típica de países tropicais e subdesenvolvidos. Devido a grande abrangência da doença, somada a dificuldade encontrada pelos pacientes para adquirir os medicamentos, Chung et al. (1996) sintetizaram o pró-fármaco hidroximetilnitrofural (NFOH). Esse derivado do nitrofural (NF), em estudos preliminares, apresentou resultados significativamente superiores aos observados com benznidazol e nitrofural, disponíveis para a terapia convencional. O presente estudo teve como objetivo a avaliação preliminar do potencial de hepatotoxicidade e cardiotoxicidade do NFOH quando administrado a coelhos albinos. O protocolo experimental se deu através da avaliação da atividade sérica dos biomarcadores hepáticos AST, ALT, GGT e bilirrubina, e do cardíaco CK-MB. Os animais foram divididos em três grupos (n=5), que receberam NFOH, NF e o veículo DMSO duas vezes ao dia, durante 24 dias. Para realização das análises foram coletadas amostras de sangue a cada seis dias. Os testes foram realizados através de kits bioquímicos para diagnóstico (Labtest®). Os níveis de AST e ALT dos grupos expostos aos fármacos apresentaram significativa diminuição com relação ao grupo controle, a bilirrubina não apresentou alterações significativas, e a atividade sérica da GGT apresentou-se elevada tanto nos grupos NFOH e NF quanto no grupo controle. Foi observada alta mortalidade entre os animais expostos ao NF e os valores de CK-MB foram significativamente superiores aos valores de referência descritos em literatura, sugerindo dano cardíaco. Tendo como base tais resultados, concluímos que o pró-fármaco não originou prejuízos relacionados ao tecido hepático, porém com relação à cardiotoxicidade há necessidade de estudos posteriores.

Lipidose hepática felina

Feline hepatic lipidosis or fatty liver disease is a cholestatic syndrome that affects domestic cats and is characterized by excess fat accumulation in the liver of cats. Symptoms commonly seen with this syndrome are anorexia, weight loss, lethargy, vomiting, jaundice, and occasionally behavioral or neurologic signs such as excessive drooling, blindness, coma, and seizures. The diagnosis is based on the patient history, clinical examination, complementary examination, and the definitive diagnosis is obtained by cytology and/or histopathology of hepatic tissue. In serum biochemistry, the main findings include increased serum alkaline phosphatase (ALP), alanine aminotransferase (ALT), aspartate amino transferase (AST) and bilirubin. The gamma glutamyl transferase (GGT) is normal or slightly increased. The cornerstone of therapy is aggressive feeding to supply the cat full caloric requirements. Without aggressive nutritional support and intensive monitoring the fatty liver disease can be fatal

Congelação de células espermáticas provenientes de epidídimo de gatos domésticos contendo antioxidante no meio diluidor

Pós-graduação em Medicina Veterinária - FCAV

Ajuste metabólico e respostas imunes de pacus juvenis alimentados com diferentes níveis de carboidratos e submetidos a jejum prolongado

Pós-graduação em Zootecnia - FCAV

Condição corporal e jejum sobre o metabolismo energético e crescimento de juvenis de pacu

Pós-graduação em Zootecnia - FCAV

Efeitos do fipronil sobre o metabolismo energético no fígado perfundido de rato: Hyllana Catarine Dias de Medeiros. -

Pós-graduação em Ciência Animal - FMVA

Avaliação ultrassonográfica hepática ao modo-B, dúplex e tríplex Doppler de cães com sobrepeso e obesos

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Cholangiocarcinoma in an American Rhea (Rhea Americana araneipes)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Efeitos do treinamento físico sobre o estresse oxidativo em fígado e pâncreas de ratos diabéticos

Pós-graduação em Ciências da Motricidade - IBRC