Serological and Genetic Evidence for Altered Complement System Functionality in Systemic Lupus Erythematosus: Findings of the GAPAID Consortium

Systemic lupus erythematosus is a chronic autoimmune disease with multifactorial ethiopathogenesis. The complement system is involved in both the early and late stages of disease development and organ damage. To better understand autoantibody mediated complement consumption we examined ex vivo immune complex formation on autoantigen arrays. We recruited patients with SLE (n = 211), with other systemic autoimmune diseases (n = 65) and non-autoimmune control subjects (n = 149). Standard clinical and laboratory data were collected and serum complement levels were determined. The genotype of SNP rs1143679 in the ITGAM gene was also determined. Ex vivo formation of immune complexes, with respect to IgM, IgG, complement C4 and C3 binding, was examined using a functional immunoassay on autoantigen microarray comprising nucleic acids, proteins and lipids. Complement consumption of nucleic acids increased upon binding of IgM and IgG even when serum complement levels were decreased due to consumption in SLE patients. A negative correlation between serum complement levels and ex vivo complement deposition on nucleic acid autoantigens is demonstrated. On the contrary, complement deposition on tested protein and lipid autoantigens showed positive correlation with C4 levels. Genetic analysis revealed that the non-synonymous variant rs1143679 in complement receptor type 3 is associated with an increased production of anti-dsDNA IgG antibodies. Notwithstanding, homozygous carriers of the previously reported susceptible allele (AA) had lower levels of dsDNA specific IgM among SLE patients. Both the non-synonymous variant rs1143679 and the high ratio of nucleic acid specific IgG/IgM were associated with multiple organ involvement. In summary, secondary complement deficiency in SLE does not impair opsonization of nucleic-acid-containing autoantigens but does affect other antigens and potentially other complement dependent processes. Dysfunction of the receptor recognizing complement opsonized immune complexes promotes the development of class-switched autoantibodies targeting nucleic acids.

Multiple single unit recording in the cortex of monkeys using independently moveable microelectrodes.

Simultaneous recording from multiple single neurones presents many technical difficulties. However, obtaining such data has many advantages, which make it highly worthwhile to overcome the technical problems. This report describes methods which we have developed to permit recordings in awake behaving monkeys using the 'Eckhorn' 16 electrode microdrive. Structural magnetic resonance images are collected to guide electrode placement. Head fixation is achieved using a specially designed headpiece, modified for the multiple electrode approach, and access to the cortex is provided via a novel recording chamber. Growth of scar tissue over the exposed dura mater is reduced using an anti-mitotic compound. Control of the microdrive is achieved by a computerised system which permits several experimenters to move different electrodes simultaneously, considerably reducing the load on an individual operator. Neurones are identified as pyramidal tract neurones by antidromic stimulation through chronically implanted electrodes; stimulus control is integrated into the computerised system. Finally, analysis of multiple single unit recordings requires accurate methods to correct for non-stationarity in unit firing. A novel technique for such correction is discussed.

A record production from an integrated farming system utilising sewage enriched water

The results of experiments conducted on a pond dyke (655m²) in the Wastewater Aquaculture Division of the Central Institute of Freshwater Aquaculture, Rahara, during 1992-93 for maximising production through optimum utilisation of resources are communicated. Round the year intensive cultivation of okra (Abelmoschus esculentus), amaranth (Amaranthus gangeticus and A. viridus), water-bind weed (Ipomea aquatica), Indian spinach (Basella rubra), radish (Raphanus sativum), amaranth (Amaranthus viridis), cauliflower (Brassica oleracia var. votrytis), cabbage (Brassica oleracia var. capitota) and papaya (Carica papaya) was undertaken using the treated sewage water from fish ponds for irrigation. The pond dyke yielded 5,626.5 kg vegetable which worked out to 85.9 tons per ha per year. Multiple cropping with these vegetables excluding papaya on a 460 m² dyke recorded a production of 4,926.5 kg at the rate of 107.1t per ha/yr. An improved yearly net return of about 35% over investment could be achieved through the selection of highly productive and pest resistant vegetable crops of longer duration for integration into the system. Introduction of this type of integrated farming would enhance the overall productivity and returns from farming.

The use of syntax and multiple alternatives in the VODIS voice operated database inquiry system

The paper describes the architecture of VODIS, a voice operated database inquiry system, and presents some experiments which investigate the effects on performance of varying the level of a priori syntactic constraints. The VODIS system includes a novel mechanism for incorporating context-free grammatical constraints directly into the word recognition algorithm. This allows the degree of a priori constraint to be smoothly varied and provides for the controlled generation of multiple alternatives. The results show that when the spoken input deviates from the predefined task grammar, a combination of weak a priori syntax rules in conjunction with full a posteriori parsing on a lattice of alternative word matches provides the most robust recognition performance. © 1991.

A single all-optical processor for multiple spectral amplitude code label recognition using four wave mixing

We propose a novel label processor which can recognize multiple spectral-amplitude-code labels using four-wave-mixing sidebands and selective optical filtering. Ten code-labels x 10 Gbps variable-length packets are transmitted over a 200 km single-hop switched network.

Tandem system adaptation using multiple linear feature transforms

Adaptation to speaker and environment changes is an essential part of current automatic speech recognition (ASR) systems. In recent years the use of multi-layer percpetrons (MLPs) has become increasingly common in ASR systems. A standard approach to handling speaker differences when using MLPs is to apply a global speaker-specific constrained MLLR (CMLLR) transform to the features prior to training or using the MLP. This paper considers the situation when there are both speaker and channel, communication link, differences in the data. A more powerful transform, front-end CMLLR (FE-CMLLR), is applied to the inputs to the MLP to represent the channel differences. Though global, these FE-CMLLR transforms vary from time-instance to time-instance. Experiments on a channel distorted dialect Arabic conversational speech recognition task indicates the usefulness of adapting MLP features using both CMLLR and FE-CMLLR transforms. © 2013 IEEE.

Low cost optical access networks using 20 Gchip/s electronic CMDA

This paper describes electronically processed CDMA techniques which allow Gb/s data rates for each user in passive optical networks. We will present recent progress including a 16 chip Walsh-code system operating at 18 Gchip/s supporting up to 16 users. © 2009 IEEE.

16 chip, 18 Gchips/s walsh-code implementation of an ECDMA access network using two time-shifted FIR filters

We demonstrate for the first time an electronically processed Walsh Code with 16 chips at 18Gchip/s. An auto-cross correlation ratio of 18.1dB is achieved between two orthogonal codes after transmission over 10km of SMF. © 2009 OSA.

Digital radio distribution system over multiple cabling types

This paper demonstrates a novel digital radio distribution system able to transmit not only over optical fibres and coaxial cables but also over twisted pair cables. The digitised RF signal is compressed for maximum transmission efficiency in a way that allows for integral self-learning algorithms to be introduced for multi-service applications. © 2013 IEEE.

a state-adaptive access control model for web-based idrs system

Huazhong Univ Sci & Technol, Natl Tech Univ Ukraine, Huazhong Normal Univ, Harbin Inst Technol, IEEE Ukraine Sect, I& M/CI Joint Chapter

A Multiple-Context Equality-Based Reasoning System

Expert systems are too slow. This work attacks that problem by speeding up a useful system component that remembers facts and tracks down simple consequences. The redesigned component can assimilate new facts more quickly because it uses a compact, grammar-based internal representation to deal with whole classes of equivalent expressions at once. It can support faster hypothetical reasoning because it remembers the consequences of several assumption sets at once. The new design is targeted for situations in which many of the stored facts are equalities. The deductive machinery considered here supplements stored premises with simple new conclusions. The stored premises include permanently asserted facts and temporarily adopted assumptions. The new conclusions are derived by substituting equals for equals and using the properties of the logical connectives AND, Or, and NOT. The deductive system provides supporting premises for its derived conclusions. Reasoning that involves quantifiers is beyond the scope of its limited and automatic operation. The expert system of which the reasoning system is a component is expected to be responsible for overall control of reasoning.