Nueva vía de conexión entre la A-1, A-6 y A-5. Tramo: Las Rozas – M-503 (Madrid)

Este Proyecto se enmarca en el conjunto de actuaciones necesarias para desarrollar por completo la denominada Autovía de circunvalación M-55,que conecta la A-1, A-6 y A-5, y que contribuye a mejorar la capacidad de los corredores que comunican la ciudad de Madrid con los municipios metropolitanos comprendidos entre la A-1, A-6 y A-5, y también a estos municipios entre sí. El área de estudio se encuentra ubicada en la Comunidad de Madrid,incluyendo los municipios de Las Rozas de Madrid y Villanueva del Pardillo. El tramo discurre por una zona protegida medioambientalmente como es el LIC “Cuenca del río Guadarrama” y próximo a los núcleos de población de Las Roza de Madrid y Villanueva del Pardillo. Por ello, se ha buscado optimizar el trazado minimizando las afecciones a edificaciones y al espacio protegido medioambientalmente por la Red Natura 2000.

Processing and validation of JEFF3-3.1.2 Cross-section Library into Various Formats: ACE, PENDF, GENDF, MATXSR and BOXER.

Following the processing and validation of JEFF-3.1 performed in 2006 and presented in ND2007, and as a consequence of the latest updated of this library (JEFF-3.1.2) in February 2012, a new processing and validation of JEFF-3.1.2 cross section library is presented in this paper. The processed library in ACE format at ten different temperatures was generated with NJOY-99.364 nuclear data processing system. In addition, NJOY-99 inputs are provided to generate PENDF, GENDF, MATXSR and BOXER formats. The library has undergone strict QA procedures, being compared with other available libraries (e.g. ENDF/B-VII.1) and processing codes as PREPRO-2000 codes. A set of 119 criticality benchmark experiments taken from ICSBEP-2010 has been used for validation purposes.

Induction of epithelial chloride secretion by channel-forming cryptdins 2 and 3

Salt and water secretion from intestinal epithelia requires enhancement of anion permeability across the apical membrane of Cl− secreting cells lining the crypt, the secretory gland of the intestine. Paneth cells located at the base of the small intestinal crypt release enteric defensins (cryptdins) apically into the lumen. Because cryptdins are homologs of molecules known to form anion conductive pores in phospholipid bilayers, we tested whether these endogenous antimicrobial peptides could act as soluble inducers of channel-like activity when applied to apical membranes of intestinal Cl− secreting epithelial cells in culture. Of the six peptides tested, cryptdins 2 and 3 stimulated Cl− secretion from polarized monolayers of human intestinal T84 cells. The response was reversible and dose dependent. In contrast, cryptdins 1, 4, 5, and 6 lacked this activity, demonstrating that Paneth cell defensins with very similar primary structures may exhibit a high degree of specificity in their capacity to elicit Cl− secretion. The secretory response was not inhibited by pretreatment with 8-phenyltheophyline (1 μM), or dependent on a concomitant rise in intracellular cAMP or cGMP, indicating that the apically located adenosine and guanylin receptors were not involved. On the other hand, cryptdin 3 elicited a secretory response that correlated with the establishment of an apically located anion conductive channel permeable to carboxyfluorescein. Thus cryptdins 2 and 3 can selectively permeabilize the apical cell membrane of epithelial cells in culture to elicit a physiologic Cl− secretory response. These data define the capability of cryptdins 2 and 3 to function as novel intestinal secretagogues, and suggest a previously undescribed mechanism of paracrine signaling that in vivo may involve the reversible formation of ion conductive channels by peptides released into the crypt microenvironment.

Glycine-induced long-term potentiation is associated with structural and functional modifications of α-amino-3-hydroxyl-5-methyl-4-isoxazolepropionic acid receptors

Global long-term potentiation (LTP) was induced in organotypic hippocampal slice cultures by a brief application of 10 mM glycine. Glycine-induced LTP was occluded by previous theta burst stimulation-induced potentiation, indicating that both phenomena share similar cellular processes. Glycine-induced LTP was associated with increased [3H]α-amino-3-hydroxyl-5-methyl-4-isoxazolepropionic acid (AMPA) binding in membrane fractions as well as increased amount of a selective spectrin breakdown product generated by calpain-mediated spectrin proteolysis. Antibodies against the C-terminal (C-Ab) and N-terminal (N-Ab) domains of GluR1 subunits were used to evaluate structural changes in AMPA receptor properties resulting from glycine-induced LTP. No quantitative or qualitative changes were observed in Western blots from membrane fractions prepared from glycine-treated slices with C-Ab. In contrast, Western blots stained with N-Ab revealed the formation of a 98-kDa species of GluR1 subunits as well as an increased amount of immunoreactivity after glycine-induced LTP. The amount of spectrin breakdown product was positively correlated with the amount of the 98-kDa species of GluR1 after glycine treatment. Functional modifications of AMPA receptors were evaluated by determining changes in the effect of pressure-applied AMPA on synaptic responses before and after glycine-induced LTP. Glycine treatment produced a significant increase in AMPA receptor function after potentiation that correlated with the degree of potentiation. The results indicate that LTP induction produces calpain activation, truncation of the C-Ab domain of GluR1 subunits of AMPA receptors, and increased AMPA receptor function. They also suggest that insertion of new receptors takes place after LTP induction.