Turismo rural: uma viagem de inclusão produtiva

Pós-graduação em Serviço Social - FCHS

Entre bois e cabras: uma visão histórica sobre mentalidades e valores nos sertões

A culture of resistance in the semi-arid, expressed by mentalities and values, goes against the economic potential of sheep and goat raising, considered as an inferior activity when compared to the cattle raising, historically linked to social status and prestige. This paper attempts to shed light on aspects that make possible to clarify the roots of the cognitive apparatus that supports this view, resorting to it to the production of relevant authors dealing with the issue of displacement of colonizer and livestock in the hinterlands. Ultimately, it seeks evidence of the influence of the historical process of economic and social organization of space on the present.

Equívocos na leitura da teoria de Jean Piaget

Dans cet article, nous présentons l'analyse des erreurs commises par rapport à l'épistémologie génétique et la psychologie de Jean Piaget. Nous nous appuyons sur un essai publié dans un périodique brésilien concernant la constitution du sujet d’enfance et la théorie de Piaget. Nous avons comme but d’aider à reprendre la «vraie» théorie de Piaget et de donner un aperçu aux lecteurs à fin de ne pas reproduire de telles erreurs ou être confrontés à de différents sens donnés à la même théorie. En ce qui concerne l'analyse, les auteurs du rapport disent que l'homme est le résultat de différents stades de développement et de ses phases. Cependant, en cherchant à expliquer comment les structures logiques deviennent nécessaires, Piaget a affirmé de ne pas rien avoir inventé. Il a conclu que la constitution des structures opératoires est le résultat de l'interaction du sujet avec le milieu, à condition qu'il dispose de certaines conditions prealables et qu’il soit capable d' établir ses rapports De cette manière, s'il a identifié des moments de la psychogenèse cognitive, il l’a fait grace à la constatation de que les sujets, dans cette interaction avec le milieu, finissaient par construire des manières qualitativement différentes de comprendre et gérer la réalité. Ainsi, Piaget n'a pas cherché de mettre les individus dans des phases ou périodes, artificiellement. Une autre erreur est celui de considérer que Piaget soit adepte de la dicotomie normalité/ anormalité. Ses préoccupations sont de nature épistémologique, et s'il est parvenu à formuler une psychologie, cela avait comme but de donner une base scientifique à sa théorie de la connaissance. Encore une autre confusion a lieu quand on affirme que Piaget a identifié les enfants aux peuples primitifs. Il a seulement dit que la nécessité d’entrer en relation avec le milieu est égale pour l'homme primitif comme pour celui d'aujourd'hui; par conséquent, le fonctionnement est le seul élément biologique considéré par Piaget, vu que tous les êtres vivants cherchent à s’adapter à la réalité. D’après cet angle, on constate que, de la même manière que l’homme primitif a été un enfant - comme ceux d’aujourd'hui - il a cherché à interagir avec le milieu à fin d'assurer sa survie. Il arrive que ses demandes étaient élémentaires du point de vue logico-mathématique, contrairement à nos jours. Une autre erreur consiste à dire qu'il a soupçonné ou admis que les processus de pensée reconnaissaient une organisation logique. Il ne s’agit pas de soupçon ni d'admission, mais d´une nécéssité. Faute de cette fondation, tout le bâtiment piagétien s’effondrerait. Nous concluons que le concept de progrès et l'état de l'intelligence sous-entendent la critique des auteurs. Malgré cela, et même pas sans admettre les explications formulées par Piaget, c’est le fait de que les censeurs doivent faire face à une vérité: indépendamment de la culture ou du moment historique, les enfants agissent toujours de la même manière, en cherchant à donner un sens à la réalité. Nous pouvons donc être d’accord ou en désaccord avec l'explication de Piaget. Cependant, il y a quelque chose qui ne doit pas être ignorée: la contribution apportée par Piaget vers la compréhension de la logique du fonctionnement mental.

Relação entre poesia e prosa de Charles Baudelaire

Pós-graduação em Estudos Literários - FCLAR

O uso do treino de habilidades sociais em pessoas com fobia social na terapia comportamental

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

O uso do treino de habilidades sociais em pessoas com fobia social na terapia comportamental

The effects of an intervention in social skills were assessed in four university students with ages between 19 and 31, with social phobia diagnosis. The performance in social skills as well as performance-compatible disorder was assessed before, after the intervention, and follow-up in four months later, by the instruments: SCID-I, Mini-SPIN, QACC-VU and IHS in a multiple baseline design among participants. The intervention programmed twelve weekly meetings in which the researcher discussed socially skilled alternatives to the described difficulties; leaded dialogued theoretical explanations about the theme of the meeting and also performed functional assessments and repertory training of the participants. It was observed deficits such as public speaking, starting and maintaining conversation in the participants’ social skills repertory, which are consistent with the criteria for social phobia and the difficulties into the adapting to university. After the intervention, the participants left difficulties in public speaking, performance characteristic compatible with the social phobia diagnosis and they also increased social repertory, although it is still necessary the improvement of other skills, such as expressing positive feeling.

Construção, aplicação e análise de proposta de formação continuada de professores para o ensino de história a alunos com deficiência intelectual

Pós-graduação em Educação - FFC

Preparation and evaluation of the electrospun chitosan/PEO fibers for potential applications in cartilage tissue engineering

Fibrous materials have morphological similarities to natural cartilage extracellular matrix and have been considered as candidate for bone tissue engineering scaffolds. In this study, we have evaluated a novel electrospun chitosan mat composed of oriented sub-micron fibers for its tensile property and biocompatibility with chondrocytes (cell attachment, proliferation and viability). Scanning electronic microscope images showed the fibers in the electrospun chitosan mats were indeed aligned and there was a slight cross-linking between the parent fibers. The electrospun mats have significantly higher elastic modulus (2.25 MPa) than the cast films (1.19 MPa). Viability of cells on the electrospun mat was 69% of the cells on tissue-culture polystyrene (TCP control) after three days in culture, which was slightly higher than that on the cast films (63% of the TCP control). Cells on the electrospun mat grew slowly the first week but the growth rate increased after that. By day 10, cell number on the electrospun mat was almost 82% that of TCP control, which was higher than that of cast films (56% of TCP). The electrospun chitosan mats have a higher Young’s modulus (P <0.01) than cast films and provide good chondrocyte biocompatibility. The electrospun chitosan mats, thus, have the potential to be further processed into three-dimensional scaffolds for cartilage tissue repair.

Trends, drivers and impacts of changes in swidden cultivation in tropical forest-agriculture frontiers: A global assessment

This meta-analysis of land-cover transformations of the past 10-15 years in tropical forest-agriculture frontiers world-wide shows that swidden agriculture decreases in landscapes with access to local, national and international markets that encourage cattle production and cash cropping, including biofuels. Conservation policies and practices also accelerate changes in swidden by restricting forest clearing and encouraging commercial agriculture. However, swidden remains important in many frontier areas where farmers have unequal or insecure access to investment and market opportunities, or where multi-functionality of land uses has been preserved as a strategy to adapt to current ecological, economic and political circumstances. In some areas swidden remains important simply because intensification is not a viable choice, for example when population densities and/or food market demands are low. The transformation of swidden landscapes into more intensive land uses has generally increased household incomes, but has also led to negative effects on the social and human capital of local communities to varying degrees. From an environmental perspective, the transition from swidden to other land uses often contributes to permanent deforestation, loss of biodiversity, increased weed pressure, declines in soil fertility, and accelerated soil erosion. Our prognosis is that, despite the global trend towards land use intensification, in many areas swidden will remain part of rural landscapes as the safety component of diversified systems, particularly in response to risks and uncertainties associated with more intensive land use systems. (C) 2011 Elsevier Ltd. All rights reserved.

Performance of rest myocardial perfusion imaging in the management of acute chest pain in the emergency room in developing nations (PREMIER trial)

Background. Rest myocardial perfusion imaging (MPI) is effective in managing patients with acute chest pain in developed countries. We aimed to define the role and feasibility of rest MPI in low-to-middle income countries. Methods and Results. Low-to-intermediate risk patients (n = 356) presenting with chest pain to ten centers in eight developing countries were injected with a Tc-99m-based tracer, and standard imaging was performed. The primary outcome was a composite of death, non-fatal myocardial infarction (MI), recurrent angina, and coronary revascularization at 30 days. Sixty-nine patients had a positive MPI (19.4%), and 52 patients (14.6%) had a primary outcome event. An abnormal rest-MPI result was the only variable which independently predicted the primary outcome [adjusted odds ratio (OR) 8.19, 95% confidence interval 4.10-16.40, P = .0001]. The association of MPI result and the primary outcome was stronger (adjusted OR 17.35) when only the patients injected during pain were considered. Rest-MPI had a negative predictive value of 92.7% for the primary outcome, improving to 99.3% for the hard event composite of death or MI. Conclusions. Our study demonstrates that rest-MPI is a reliable test for ruling out MI when applied to patients in developing countries. (J Nucl Cardiol 2012;19:1146-53.)

Biochemical, physicochemical and molecular characterization of a genuine 2-Cys-peroxiredoxin purified from cowpea [Vigna unguiculata (L.) Walpers] leaves

Background: Peroxiredoxins have diverse functions in cellular defense-signaling pathways. 2-Cys-peroxiredoxins (2-Cys-Prx) reduce H2O2 and alkyl-hydroperoxide. This study describes the purification and characterization of a genuine 2-Cys-Prx from Vigna unguiculata (Vu-2-Cys-Prx). Methods: Vu-2-Cys-Prx was purified from leaves by ammonium sulfate fractionation, chitin affinity and ion exchange chromatography. Results: Vu-2-Cys-Prx reduces H2O2 using NADPH and DTT. Vu-2-Cys-Prx is a 44 kDa (SDS-PAGE)/46 kDa (exclusion chromatography) protein that appears as a 22 kDa molecule under reducing conditions, indicating that it is a homodimer linked intermolecularly by disulfide bonds and has a pI range of 4.56-4.72; its NH2-terminal sequence was similar to 2-Cys-Prx from Phaseolus vulgaris (96%) and Populus tricocarpa (96%). Analysis by ESI-Q-TOF MS/MS showed a molecular mass/pI of 28.622 kDa/5.18. Vu-2-Cys-Prx has 8% alpha-helix, 39% beta-sheet, 22% of turns and 31% of unordered forms. Vu-2-Cys-Prx was heat stable, has optimal activity at pH 7.0, and prevented plasmid DNA degradation. Atomic force microscopy shows that Vu-2-Cys-Prx oligomerized in decamers which might be associated with its molecular chaperone activity that prevented denaturation of insulin and citrate synthase. Its cDNA analysis showed that the redox-active Cys(52) residue and the amino acids Pro(45), Thr(49) and Arg(128) are conserved as in other 2-Cys-Prx. General significance: The biochemical and molecular features of Vu-2-Cys-Prx are similar to other members of 2-Cys-Prx family. To date, only one publication reported on the purification of native 2-Cys-Prx from leaves and the subsequent analysis by N-terminal Edman sequencing, which is crucial for construction of stromal recombinant 2-Cys-Prx proteins. (C) 2012 Elsevier B.V. All rights reserved.

L’éducation peut être thérapeutique: lugar de vida et l’inclusion scolaire au Brésil d’enfants ayant des troubles psychiques

L’inclusion scolaire d’enfants en situation de handicap est la norme légale au Brésil depuis 1996. Cependant, l’institution Lugar de Vida [Lieu de vie], qui a vu le jour en 1990 au sein de l’université de São Paulo, considère avant tout cette inclusion comme l’un des axes de travail d’une proposition éducative et thérapeutique pour les enfants autistes et psychotiques. Les auteurs de cette contribution sont membres fondateurs du laboratoire universitaire LEPSI 2 [Études et recherches psychanalytiques et éducationnelles sur l’enfance] auquel le centre d’éducation thérapeutique Lugar de Vida est associé. Ils présentent ici les idées qui orientent et organisent le fonctionnement du dispositif baptisé « Éducation à visée thérapeutique », dans le contexte du débat sur la redéfi nition des frontières classiques généralement établies entre soin et éducation. Ils s’appuient pour cela sur une référence singulière aux idées freudiennes en matière d’éducation.

Stime Integrali su Gruppi di Tipo H e Principio Forte di Continuazione Unica

Lo scopo di questa tesi è dimostrare il Principio Forte di Continuazione Unica per opportune soluzioni di un'equazione di tipo Schrödinger Du=Vu, ove D è il sub-Laplaciano canonico di un gruppo di tipo H e V è un potenziale opportuno. Nel primo capitolo abbiamo esposto risultati già noti in letteratura sui gruppi di tipo H: partendo dalla definizione di tali gruppi, abbiamo fornito un'utile caratterizzazione in termini "elementari" che permette di esplicitare la soluzione fondamentale dei relativi sub-Laplaciani canonici. Nel secondo capitolo abbiamo mostrato una formula di rappresentazione per funzioni lisce sui gruppi di tipo H, abbiamo dimostrato una forma forte del Principio di Indeterminazione di Heisenberg (sempre nel caso di gruppi di tipo H) e abbiamo fornito una formula per la variazione prima dell'integrale di Dirichlet associato a Du=Vu. Nel terzo capitolo, infine, abbiamo analizzato le proprietà di crescita di funzioni di frequenza, utili a dimostrare le stime integrali che implicano in modo piuttosto immediato il Principio Forte di Continuazione Unica, principale oggetto del nostro studio.

Selective REM sleep deprivation in narcolepsy

Narcolepsy is characterized by excessive daytime sleepiness and rapid eye movement (REM) sleep abnormalities, including cataplexy. The aim of this study was to assess REM sleep pressure and homeostasis in narcolepsy. Six patients with narcolepsy and six healthy controls underwent a REM sleep deprivation protocol, including one habituation, one baseline, two deprivation nights (D1, D2) and one recovery night. Multiple sleep latency tests (MSLTs) were performed during the day after baseline and after D2. During D1 and D2 REM sleep was prevented by awakening the subjects at the first polysomnographic signs of REM sleep for 2 min. Mean sleep latency and number of sleep-onset REM periods (SOREMs) were determined on all MSLT. More interventions were required to prevent REM sleep in narcoleptics compared with control subjects during D1 (57 ± 16 versus 24 ± 10) and D2 (87 ± 22 versus 35 ± 8, P = 0.004). Interventions increased from D1 to D2 by 46% in controls and by 53% in narcoleptics (P < 0.03). Selective REM sleep deprivation was successful in both controls (mean reduction of REM to 6% of baseline) and narcoleptics (11%). Both groups had a reduction of total sleep time during the deprivation nights (P = 0.03). Neither group had REM sleep rebound in the recovery night. Narcoleptics had, however, an increase in the number of SOREMs on MSLT (P = 0.005). There was no increase in the number of cataplexies after selective REM sleep deprivation. We conclude that: (i) REM sleep pressure is higher in narcoleptics; (ii) REM sleep homeostasis is similar in narcoleptics and controls; (iii) in narcoleptics selective REM sleep deprivation may have an effect on sleep propensity but not on cataplexy.

A venom-derived neurotoxin, CsTx-1, from the spider Cupiennius salei exhibits cytolytic activities

CsTx-1, the main neurotoxic acting peptide in the venom of the spider Cupiennius salei, is composed of 74 amino acid residues, exhibits an inhibitory cysteine knot motif, and is further characterized by its highly cationic charged C terminus. Venom gland cDNA library analysis predicted a prepropeptide structure for CsTx-1 precursor. In the presence of trifluoroethanol, CsTx-1 and the long C-terminal part alone (CT1-long; Gly-45-Lys-74) exhibit an α-helical structure, as determined by CD measurements. CsTx-1 and CT1-long are insecticidal toward Drosophila flies and destroys Escherichia coli SBS 363 cells. CsTx-1 causes a stable and irreversible depolarization of insect larvae muscle cells and frog neuromuscular preparations, which seem to be receptor-independent. Furthermore, this membranolytic activity could be measured for Xenopus oocytes, in which CsTx-1 and CT1-long increase ion permeability non-specifically. These results support our assumption that the membranolytic activities of CsTx-1 are caused by its C-terminal tail, CT1-long. Together, CsTx-1 exhibits two different functions; as a neurotoxin it inhibits L-type Ca(2+) channels, and as a membranolytic peptide it destroys a variety of prokaryotic and eukaryotic cell membranes. Such a dualism is discussed as an important new mechanism for the evolution of spider venomous peptides.