Ecological condition of coastal ocean waters of the northwestern Gulf of Mexico: 2011

In August 2011, the NOAA National Ocean Service (NOS) conducted an assessment of the status of ecological condition of soft-bottom habitat and overlying waters of the continental shelf in the northwestern Gulf of Mexico (GOM). The original sampling design included 50 randomly selected sites from the Mississippi River delta to the U.S./Mexican border, representing a total area of 111,162 square kilometers; however, vessel failures and inclement weather precluded sampling at 16 sites in the western-most part of the study region. Sampling was completed at the remaining 34 sites in offshore waters between the Mississippi River delta and Freeport, Texas, representing an estimated 75,591 square kilometers. Field sampling followed standard methods and indicators applied in prior NOAA coastal studies and EPA’s Environmental Monitoring and Assessment Program (EMAP) and National Coastal Assessment (NCA). A key feature adopted from these studies was the incorporation of a random probabilistic sampling design. Such a design provides a basis for making unbiased statistical estimates of the spatial extent of ecological condition relative to various measured indicators and corresponding thresholds of concern. Indicators included multiple measures of water quality, sediment quality, and biological condition (benthic fauna, fish tissue contaminant levels). Water depths ranged from 13 – 83 m throughout the study area. About 9 % of the area had sediments composed of sands (< 20 % silt+clay), 47 % of the area was composed of intermediate muddy sands (20 – 80 % silt+clay), and 44 % of the sampled area consisted of mud (> 80 % silt+clay). About 50 % of the area (represented by 17 sites) had sediment total organic carbon (TOC) concentrations < 5 mg/g and all of the sites sampled had levels of TOC < 20 mg/g, well below the range associated with potentially harmful effects to benthic fauna (> 50 mg/g). Surface salinities ranged from 23.4 – 36.5 psu, with salinity generally increasing with distance west of the Mississippi River delta. Bottom salinities varied between 31.1 and 36.5 psu, with lowest values occurring at shallow, inner-shelf stations. Surface-water temperatures varied between 29.8 and 31.5 ºC, while near-bottom waters ranged in temperature from 19.4 – 31 ºC. An index of density stratification (Δσt) indicated that portions of coastal shelf waters in the northwestern GOM at the time of this sampling were strongly stratified. Values of Δσt at 19 of the 34 sites sampled in this study (56 % of the study area) ranged from 2.2 to 12.4, which is within the range considered to be indicative of strong vertical stratification (Δσt > 2). Stratification was strongest close to the Mississippi River delta, and decreased with distance west of the delta.

Ecological Condition of Coastal Ocean Waters within Stellwagen Bank National Marine Sanctuary: 2008

In June 2008, the NOAA National Ocean Service (NOS), in conjunction with the EPA National Health and Environmental Effects Laboratory (NHEERL), conducted an assessment of the status of ecological condition of soft-bottom habitat and overlying waters within the boundaries of Stellwagen Bank National Marine Sanctuary (SBNMS). The sanctuary lies approximately 20 nautical miles east of Boston, MA in the southwest Gulf of Maine between Cape Ann and Cape Cod and encompassing 638 square nautical miles (2,181 km2). A total of 30 stations were targeted for sampling using standard methods and indicators applied in prior NOAA coastal studies and EPA’s Environmental Monitoring and Assessment Program (EMAP) and National Coastal Assessment (NCA). A key feature adopted from these studies was the incorporation of a random probabilistic sampling design. Such a design provides a basis for making unbiased statistical estimates of the spatial extent of ecological condition relative to various measured indicators and corresponding thresholds of concern. Indicators included multiple measures of water quality, sediment quality, and biological condition (benthic fauna, fish tissue contaminant levels). Depths ranged from 31 – 137 m throughout the study area. About 76 % of the area had sediments composed of sands (< 20 % silt-clay), 17 % of the area was composed of intermediate muddy sands (20 – 80 % silt-clay), and 7 % of the sampled area consisted of mud (> 80 % siltclay). About 70 % of the area (represented by 21 sites) had sediment total organic carbon (TOC) concentrations < 5 mg/g and all but one site (located in Stellwagen Basin) had levels of TOC < 20 mg/g, which is well below the range potentially harmful to benthic fauna (> 50 mg/g). Surface salinities ranged from 30.6 – 31.5 psu, with the majority of the study region (approximately 80 % of the area) having surface salinities between 30.8 and 31.4 psu. Bottom salinities varied between 32.1 and 32.5 psu, with bottom salinities at all sites having values above the range of surface salinities. Surface-water temperatures varied between 12.1 and 16.8 ºC, while near-bottom waters ranged in temperature from 4.4 – 6.2 ºC. An index of density stratification (Δσt) indicated that the waters of SBNMS were stratified at the time of sampling. Values of Δσt at 29 of the 30 sites sampled in this study (96.7 % of the study area) varied from 2.1 – 3.2, which is within the range considered to be indicative of strong vertical stratification (Δσt > 2) and typical of the western Gulf of Maine in summer. Levels of dissolved oxygen (DO) were confined to a fairly narrow range in surface (8.8 – 10.4 mg/L) and bottom (8.5 – 9.6 mg/L) waters throughout the survey area. These levels are within the range considered indicative of good water quality (> 5 mg/L) with respect to DO. None of these waters had DO at low levels (< 2 mg/L) potentially harmful to benthic fauna and fish.

Sediment quality triad assessment in Kachemak Bay: characterization of soft bottom benthic habitats and contaminant bioeffects assessment condensed. A NOAA/NOS/NCCOS/CCMA Special Report

A baseline environmental characterization of the inner Kachemak Bay, Alaska was conducted using standardized National Status and Trends Bioeffects Program methods. Three sites near the village of Port Graham were also sampled for comparison. Concentrations of over 120 organic and metallic contaminants were analyzed. Ambient toxicity was assessed using two bioassays. A detailed benthic community condition assessment was performed. Habitat parameters (e.g. depth, salinity, temperature, dissolved oxygen, sediment grain size, and organic carbon content) that influence species and contaminant distribution were also measured at each sampling site. The following is the synopsis of findings • Sediments were mostly mixed silt and sand with pockets of muddy zones. Organic compounds (PAHs, DDTs, PCBs, chlorinated pesticides) were detected throughout the bay but at relatively low concentrations. With some exceptions, metals concentrations were relatively low and probably reflect the input of glacial runoff. • Homer Harbor had elevated concentrations of metallic and organic contaminants. Concentrations of organic contaminants measured were five to ten times higher in the harbor sites than in the open bay sites. Tributyltin was elevated in Homer Harbor relative to the other areas. • There was no evidence of residual PAHs attributable to oil spills, outside of local input in the confines of the harbor. • The benthic community is very diverse. Specific community assemblages were distributed based on depth and water clarity. Species richness and diversity was lower in the eastern end of the bay in the vicinity of the Fox River input. Abundance was also generally lower in the eastern portion of the study area, and in the intertidal areas near Homer. The eastern portions of the bay are stressed by the sediment load from glacial meltwater. • Significant toxicity was virtually absent. • The benthic fauna at Port Graham contained a significant number of species not found in Kachemak Bay. • Selected metal concentrations were elevated at Port Graham relative to Kachemak Bay, probably due to local geology. Organic contaminants were elevated at a site south of the village.

Sediment Quality Triad assessment in Kachemak Bay: characterization of soft bottom benthic habitats and contaminant bioeffects assessment

A baseline environmental characterization of the inner Kachemak Bay, Alaska was conducted using the sediment quality triad approach based on sediment chemistry, sediment toxicity, and benthic invertebrate community structure. The study area was subdivided into 5 strata based on geophysical and hydrodynamic patterns in the bay (eastern and western intertidal mud flats, eastern and western subtidal, and Homer Harbor). Three to seven locations were synoptically sampled within each stratum using a stratified random statistical design approach. Three sites near the village of Port Graham and two sites in the footprint of a proposed Homer Harbor expansion were also collected for comparison. Concentrations of over 120 organic and metallic contaminants were analyzed. Ambient toxicity was assessed using two amphipod bioassays. A detailed benthic community condition assessment was performed. Habitat parameters (depth, salinity, temperature, dissolved oxygen, sediment grain size, and organic carbon content) that influence species and contaminant distribution were also measured at each sampling site. Sediments were mostly mixed silt and sand; characteristic of high energy habitats, with pockets of muddy zones. Organic compounds (PAHs, DDTs, PCBs, cyclodienes, cyclohexanes) were detected throughout the bay but at relatively low concentrations. Tributyltin was elevated in Homer Harbor relative to the other strata. With a few exceptions, metals concentrations were relatively low and probably reflect the input of glacial runoff. Relative to other sites, Homer Harbor sites were shown to have elevated concentrations of metallic and organic contaminants. The Homer Harbor stratum however, is a deep, low energy depositional environment with fine grained sediment. Concentrations of organic contaminants measured were five to ten times higher in the harbor sites than in the open bay sites. Concentration of PAHs is of a particular interest because of the legacy of oil spills in the region. There was no evidence of residual PAHs attributable to oil spills, outside of local input, beyond the confines of the harbor. Concentrations were one to ten times below NOAA sediment quality guidelines. Selected metal concentrations were found to be relatively elevated compared to other data collected in the region. However, levels are still very low in the scale of NOAA’s sediment quality guidelines, and therefore appear to pose little or no ecotoxicity threat to biota. Infaunal assessment showed a diverse assemblage with more than 240 taxa recorded and abundances greater than 3,000 animals m-22 in all but a few locations. Annelid worms, crustaceans, snails, and clams were the dominant taxa accounting for 63 %, 19%, 5%, and 7 % respectively of total individuals. Specific benthic community assemblages were identified that were distributed based on depth and water clarity. Species richness and diversity was lower in the eastern end of the bay in the vicinity of the Fox River input. Abundance was also generally lower in the eastern portion of the study area, and in the intertidal areas near Homer. The eastern portions of the bay are stressed by the sediment load from glacial meltwater. Significant toxicity was virtually absent. Conditions at the sites immediately outside the existing Homer Harbor facility did not differ significantly from other subtidal locations in the open Kachemak Bay. The benthic fauna at Port Graham contained a significant number of species not found in Kachemak Bay. Contaminant conditions were variable depending on specific location. Selected metal concentrations were elevated at Port Graham and some were lower relative to Kachemak Bay, probably due to local geology. Some organic contaminants were accumulating at a depositional site.

Strategy of fish seed production in India

In tropical countries like India, climatic conditions vary in different parts, as a result the breeding of fast growing species such as Catla and silver carps poses considerable problems due to erratic or late monsoon or prolonged dry spell. Hence it is not possible to meet the fish seed requirement of the country. Therefore: to meet the future requirement and to over-come the environmental conditions a Modern carp Hatchery CIFE D-81 was designed. The system provides optimum temperature, silt free clean, cool, highly oxygenated water, low current and continuous removal of metabolites. It has been successfully demonstrated at Rawatbhata (Rajasthan), Badkhal (Haryana), Badbhada (M.P), Guntur and East Godavari District (A.P). Recently a Modern Carp Hatchery was installed in a semi-arid zone, Damdama (Haryana), during 1984, where it has produced record 1.88 crores of fish seed and worked successfully.

Assessment of siltation rate and sediment transport in Bombay harbour based on Cesium-137 distribution

Low level aqueous radioactive wastes from the nuclear complex at Trombay are discharged into the Bombay harbour. Of the 6 principal radionuclides comprising the discharge, cesium-137 is most predominantly taken up by silt from the sea water. Isoactivity contours show that activity levels just off the point of discharge, and further south to Pir Pau, were between 50 and 100 pCi/g. Activity in the main channel of the harbour remained below 10 pCi/g, while further north (below the Thane Creek bridge) it was around 20 pCi/g. Activity in the top 6 cm of a core off Trombay naval jetty was <5 pCi/g, reaching a maximum of 178 pCi/g at 14cm below the surface. Thereafter, it tapered off to 5 pCi/g down to a metre.

Humic acids in sediments of North-Central Arabian Sea, west coast of India

Sediment samples (28) collected during the ORV Sagar Kanya cruise-29, were analysed for humic acid (HA) concentration from the North-Central Arabian Sea. Generally oceanic samples had more HA concentration than the continental shelf (< 200 m depth) samples. The photo-acoustic infrared spectra of shelf sediment HA indicated the presence of more C-H saturated aliphatic chains, while oceanic HA had few peaks for the above groups. Both the IR spectra indicated the absence of aromatic C = C, carbonyl, ketonic groups. Clayey-silt sediment generally had higher concentration of HA compared to sandy-silt type of sediment.

Distribution of surface sediments off Indus delta on the continental shelf of Pakistan

Surface sediments from the continental shelf area off Indus delta were analysed for their textural characteristics and carbonate content. The sediments are largely silt, silty clay and clayey silty sand. Sandy fraction is dominant in the outer region with relatively high carbonate content. The study shows that distribution of carbonate in sediments off Indus delta continental shelf is controlled by the dilution of terrigenous material and its distance from source area.

Deepwater prawns and lobsters off the Kerala coast

Majority of the prawn catch of the Kerala coast comes from the inshore regions within the 10 fathom line. A bottom deposit formed of fine mud rich in humus is found to be the ideal condition for the penaeid prawns. Similarly the lobster is observed in large numbers at localised areas very near the coast in the crevices of rocks. Though it has been observed that prawns and lobsters exist in deep water as well under different conditions, our knowledge about these forms along the Indian coasts is very scanty, being restricted to the works of Spence Bate, Alcock, De Man and Ramadan. Recently, during the cruises of the University Research Vessel Conch off the Kerala Coast (1958-1963) two species of deep water prawns and one species of lobster were collected from depths 100 - 180 fathoms. Of these, Penaeopsis philippi is found in large numbers occupying an almost continuous bed extending from Anjengo to Mangalore, while P. rectacutus has a restricted appearance between Cochin and Calicut. However, some sort of year to year variation has also been observed regarding the abundance of the species at various stations. Peurulus sewelli has a more restricted distribution, the maximum number being found between Puvar and Cochin. Attempt has been made to correlate the occurrence of the species with the hydrological conditions at the bottom and the nature of the substratum. It is observed that Peurulus sewelli occupies a more or less hard bed formed of sand with shell fragments or stones and small percentage of silt, the bottom temperature at the stations varying from 11°C to l4°C. P. sewelli is a more or less permanent inhabitant of the edge of the continental shell off the Kerala Coast worthy of attention for detailed investigation with a view to explore the possibilities of commercial exploitation.

Macrobenthos off Mahim (Bombay), west coast of India in relation to coastal pollution and aquaculture

Deterioration of water quality clearly indicated a moderate to severe coastal pollution around Mahim whereas a relatively healthy marine environment towards offshore was noticed. Foraminifera, polychaetes, crustaceans and pelecypods were the dominant macro faunal groups encountered in the area. The occasional inhabitants like pennatularians, nemertines, sipunculids, ophiuroids and fish larvae were mostly restricted to offshore regions. Biomass and population density of macro fauna were moderate and showed fluctuating trend. Similarity coefficients of foraminifera (0.89) and polychaetes (0.81) were high for offshore unpolluted stations (3 and 4) as compared to near shore coastal stations (1 and 2). Faunal diversity was relatively more in unpolluted zone. The diversity index (H) was more at station 3 for polychaetes (1.39) and at station 4 for foraminifers (0.54). In general, biomass was high during post monsoon excepting station 1 which was invariably under severe pollution stress throughout the study period. Foraminifera were abundant at sandy bottom while polychaetes preferred muddy (clayey silt) bottom. A noticeable ecological modification associated with faunal abundance and diversity were related to deteriorating marine water quality due to anthropogenic waste disposals. The prevailing water quality around Mahim is unsuitable for any kind of culture practices and also for harvesting economically important marine species.

Ecology and settlement of marine fouling in the Suez Bay, Egypt

This study deals with seasonal variations, natural correlations and similarities of fouling assemblages on exposure panels in the Suez Bay during January 1992 to January 1993. Three main sources of pollutions flow into the bay; industrial waste products, domestic drainage of Suez city and ships' oil and refuse.The fouling assemblages on the test pan els after various periods (1, 2 and 3 months) belonged mainly to the algae (Ulva rigida), polychaetes (Hydroides elegans), Cirripedes (Balanus amphitrite) and amphipods. The fouling at the lst station was relatively more dense than at the 2nd station during the summer and autumn seasons. The lowest productivity was achieved at the 3rd station which was considered less polluted being offshore water. The overall paucity of fouling in the bay is because of the silt covering the submerged surfaces, particularly at the 2nd station, leading to the prevention of the settlements or establishment of fouling organisms. The seasonal changes in the intensity of fouling assemblages on submerged surfaces in seawater seems to be closely related to seasonal variations in water temperature. The great fouling communities on the buoys and long exposure panels showed a remarkable variety of species and density rather than on short term exposures, which were more dense during warmer months.

Ecological imbalances in the coastal areas of Pakistan and Karachi Harbour

The marine environment of Pakistan has been described in the context of three main regions : the Indus delta and its creek system, the Karachi coastal region, and the Balochistan coast. The creeks, contrary to concerns, do receive adequate discharges of freshwater. On site observations indicate that freshwater continues flowing into them during the lean water periods and dilutes the seawater there. A major factor for the loss of mangrove forests as well as ecological disturbances in the Indus delta is loss of the silt load resulting in erosion of its mudflats. The ecological disturbance has been aggravated by allowing camels to browse the mangroves. The tree branches and trunks, having been denuded of leaves are felled for firewood. Evidence is presented to show that while indiscriminate removal of its mangrove trees is responsible for the loss of large tracts of mangrove forests, overharvesting of fisheries resources has depleted the river of some valuable fishes that were available from the delta area. Municipal and industrial effluents discharged into the Lyari and Malir rivers and responsible for land-based pollution at the Karachi coast and the harbour. The following are the three major areas receiving land-based pollution and whose environmental conditions have been examined in detail: (l) the Manora channel, located on the estuary of the Lyari river and serving as the main harbour, has vast areas forming its western and eastern backwaters characterized by mud flats and mangroves. The discharge of industrial wastewater from the S.I.T.E. and municipal effluents from the northern and central districts into the Lyari has turned this river into an open drain. This, in turn, has caused a negative impact on the environment of the port, fish harbour, and the adjacent beaches. (2) The Gizri creek receives industrial and municipal effluents from the Malir river as well as from several industries and power stations. The highly degraded discharges from the Malir have negatively impacted the environment in this creek. (3) The coastline between the Manora channel and Gizri creek where the untreated municipal effluents are discharged by the southern districts of Karachi, is responsible for the degraded environment of the Chinna creek, and also of the beaches and the harbour. The Balochistan coast is relatively safe from land-based pollution, mainly because of the lack of industrial, urban or agricultural activity, except the Hingol river system where some agricultural activities have been initiated.

Fish farm technology. 1 - Brackishwater fish culture based on "aquafeed"

A method has been evolved to enhance the production of natural feed in brackish water fish farms by providing substrates for bio-growth ('aquafeed' production) which is a biomass complex consisting of sedentary and associated organisms of plant and animal species. The seasonal fluctuations of the aquafeed production over different substrates ranged as: 787-1830g/coconut leaf (6m²)/45 days, 16.0-072.9g/glass panel (2x10x10cm²)/30 days, 52-230g/nylon mat (2x25x25cm²)/30 days and 18.6-123.1g/wooden block (6x10x10cm²)/30 days. The average dry weight composition of the major components of aquafeed obtained in the present study was sand-silt-clay 40%, protein 22%, carbohydrate (water soluble) 1.8% and fat 3.35% (water content 85%). Mugil cephalus of 1.85 cm reared in a 0.01ha pond and fed on aquafeed attained a size of 23 cm length and 146.73g weight during one year. Survival rate was 54% at a density of 1000/ha. Salinity and temperature of the pond during the culture period ranged between 1.4 and 32.8‰ and 28.1 and 36.5°C respectively.

Diversity, abundance distribution and secondary production of intertidal and subtidal benthic macrofauna of Bushehr Province with emphasis on annelida groups

The present investigation was undertaken to establish a reference situation for future use, to identify temporal and spatial composition of macrofauna and estimate some ecological indices in the sub tidal waters along the Bushehr coastal waters in Persian Gulf. Six transects were selected including Genaveh, Farakeh, Shif, Bandargah, Rostami and Asalouyeh, at each transect 3 station were sampled in depths of zero, 5 and 10 metres. Sampling was seasonally carried out by a VAN VEEN grab 0.0225 m2, during summer 2008 until spring 2009. Samples were wet sieved immediately using 0.5 mm mesh size sieves and sediment retained in the sieve was preserved in 4% buffered formalin solution. Macrofauna specimen were separated from the sediments using decantation and elutriation methods, enumerated and identified up to the Genus level. Environmental factors such as temperature. pH, and salinity were recorded in field using sensitive probs and refractometer (for salinity) and also sediment samples were taken for TOM and grain size analysis in all the stations. 5611 specimens belonging to 66 genera were collected during the present study. Polychaetes were dominant both in terms of genus number (31) and relative abundance (74 % of total macrofaunal abundance). The other dominant groups were Artheropoda, (16.1%), Molusca (2.8%), Echinodermata (1.29%) and others including Nematoda, Nemertina, Echiura and Turbellaria (5.8%). Thirty one Genera belong of 27 families of polychaeta, one genus and family of Subphylum Chlicerata,19 genera belong to 14 families of Crustacea, 8 genera belong to 6 families of Molusca, were indentified in the studied region. 1 family (Polygordidae) and 3 genera (Flabeligera, Pilargis and Polygordius) of Polychaeta, 1 family (Nymphonidae) and genus (Nymphon) of Chelicerata, 1 Family (Nematoplanidae) and genus (Nematoplana) of Turbellaria, were identified for the first time in Persian Gulf area. The result indicated that macrofauna organism have strong relationship with the grain size characteristics of the sediments they inhabit. The most surface deposit feeder specimens such as Prionospio and Cossura were found in zero meters depth of Genaveh, Farakeh, Bandargah, Rostami and Asalouyeh stations with sandy substratum, however the most burrowing deposit feeder and scavenger specimens such as Capitella and Petaloproctus were collected in 5 and 10 meter depths of stations with silty–clay substratum. The annual mean abundance, Shanon- weiner diversity and evenness of macrofauna were estimated1152.73 N/ m² , 2.72 and 0.792 respectively .The annual average biomass and secondary production were computed 1.797 gDW m² and 3.594 gDW m² y-1 .The average of water temperature, salinity, pH and oxygen concentration were recorded between 16.37-36.05 °C, 38-42 g/l, 7.89-8.76 and 4.23-8.23 mg/l, respectively during this study in 6 studied region. Among of investigated stations Asalouyeh adjacent of effluent canal of Gas and petrochemical industry sewage and Farakeh regions adjacent the Helleh estuary had the lowets and the highest community indices. The average of diversity and density in 5 meters depth stations with moderate of sand, silt and clay were slightly more than 2 other depths stations, it seems that 5 meters stations are made a transition habitats between 2 sandy and clay habitats, that can be used by 2 groups of surface and borrowing deposit feeders. Based on the data provided in this survey, the temperature variation, sediment texture, TOM, type habitat and manmade factors of Gas and petrochemical industries have had the most effect on the macrofauna community structure in the studied region during sampling periods.

The zonation of soft-bottom sediments in Gorgan Bay (Caspian Sea) using macrobenthos as indicator of ecological health

In this study, in order to assess the ecological health status and zoning of soft bottom of Gorgan Bay, the spatial and temporal distribution of macrofauna and their relationship with environmental stress were investigated. Sediment samples were collected using a Van Veen grab at 22 sampling points, seasonally during 2012-2013. The averages (±SD) of the percentages of sand, silt, clay and TOM (Total Organic Matter) in the sediment samples were determined (44.4± 15, 53.4 ± 14, and 2.2 ±2.2 and 7.2% ± 1.6, respectively). Our results showed that mean (range) of Al, As, Cu, Fe, Ni, Pb and Zn in the sediment samples were 1.2 % (0.4-2.1), 4.8 (2.5- 10.3) ppm, 10.5 (4.4-16.9) ppm, 1 (0.4 – 1.6) % , 13.6 (6.2-21.5) ppm, 9.1 (4.7-12.9) ppm and 23.9 (3.1-39.4) ppm, respectively. In spring, both Al and Ni were higher than the guideline level. In the event that arsenic was exceeds the guidelines in summer. In this study, 14 species of macrofauna from 12 families were identified. Polychaeta with 3 species was the most dominant group in terms of abundance. The four most abundant taxa making up 85% of all specimens (Streblospio gynobranchiata, Tubificidae, Hediste versicolor and Abra segmentum). The western area were characterized by the higher species diversity (H', 1.94). So Gorgan Bay presents transitional macrobenthic assemblages that are spatially distributed along substrate gradients .The mean of Shannon index, BENTIX, BO2A, AMBI and M-AMBI in the bay was 1.3, 2.2, 0.4, 3.2 and 0.65 respectively. According to the results of these indices, ecological status of the western part of the bay assessed better than the other parts. According to the results of the nmMDS (non-metric Multidimensional Scaling), PCA (Principal Components Analysis), the map of distribution of heavy metals and the map of the ecological status , it seems Gorgan Bay is divided into two separate zones (the eastern and the western parts).M-AMBI finaly introduced reliable index for assessing the ecological status of the Bay.