Caribbean shipping sector: a planning and policy perspective

Includes bibliography

Planning and the market during the next ten years in Latin America

Sostiene que la necesidad de establecer un justo equilibrio entre mercado y acción estatal, sobre la base de un diagnóstico objetivo de las virtudes y deficiencias de uno y la otra, constituye una de las grandes lecciones de la experiencia económica de la posguerra para el futuro de las relaciones entre planificación y mercado.

Planning and government

Includes bibliography

Strategic management, planning and budgets

Includes bibliography

Latin America: incorporating environmental factors into the measurement of production efficiency and technical change

Includes bibliography

The production structure and the dynamics of development

Includes bibliography

Production, purification, and characterization of a major penicillium glabrum xylanase using brewer's spent grain as substrate

In recent decades, xylanases have been used in many processing industries. This study describes the xylanase production by Penicillium glabrum using brewer's spent grain as substrate. Additionally, this is the first work that reports the purification and characterization of a xylanase using this agroindustrial waste. Optimal production was obtained when P. glabrum was grown in liquid medium in pH 5.5, at 25 °C, under stationary condition for six days. The xylanase from P. glabrum was purified to homogeneity by a rapid and inexpensive procedure, using ammonium sulfate fractionation and molecular exclusion chromatography. SDS-PAGE analysis revealed one band with estimated molecular mass of 18.36 kDa. The optimum activity was observed at 60 °C, in pH 3.0. The enzyme was very stable at 50 °C, and high pH stability was verified from pH 2.5 to 5.0. The ion Mn2+ and the reducing agents β-mercaptoethanol and DTT enhanced xylanase activity, while the ions Hg2+, Zn2+, and Cu2+ as well as the detergent SDS were strong inhibitors of the enzyme. The use of brewer's spent grain as substrate for xylanase production cannot only add value and decrease the amount of this waste but also reduce the xylanase production cost. © 2013 Adriana Knob et al.