Rapport sur le fonctionnement du Service Océanographique des Pêches de l'Indochine pendant l'année 1927-1928

The present note deals with the functions and activities done by the Institute of oceanography of Indochina during 1927-1928.

Rapport sur le fonctionnement du Service Océanographique des Pêches de l'Indochine pendant l'année 1928-1929

The present note deals with the functions and activities done by the Institute of oceanography of Indochina during 1928-1929.

Rapport sur le fonctionnement du l'Institut Océanographique de l'Indochine pendant l'année 1929-1930

The present note deals with the functions and activities done by the Institute of oceanography of Indochina during 1929-1930.

Rapport sur le fonctionnement du l'Institut Océanographique de l'Indochine pendant l'année 1930-1931

The present note deals with the functions and activities done by the Institute of oceanography of Indochina during 1930-1931.

Rapport sur le fonctionnement du l'Institut Océanographique de l'Indochine pendant l'année 1931-1932

The present note deals with the functions and activities done by the Institute of oceanography of Indochina during 1931-1932.

Rapport sur le fonctionnement de l'Institut Océanographique de l'Indochine pendant l'année 1932-1933

The present note deals with the functions and activities done by the Institute of oceanography of Indochina during 1932-1933.

Rapport sur le fonctionnement du l'Institut Océanographique de l'Indochine pendant l'année 1933-1934

The present note deals with the functions and activities done by the Institute of oceanography of Indochina during 1933-1934.

Rapport sur le fonctionnement de l'Institut Océanographique de l'Indochine pendant l'année 1934-1935

The present note deals with the functions and activities done by the Institute of oceanography of Indochina during 1934-1935.

O papel das representações na resolução de problemas de Matemática: um estudo no 1º ano de escolaridade

Este estudo tem como objectivo investigar o papel que as representações, construídas por alunos do 1.o ano de escolaridade, desempenham na resolução de problemas de Matemática. Mais concretamente, a presente investigação procura responder às seguintes questões: Que representações preferenciais utilizam os alunos para resolver problemas? De que forma é que as diferentes representações são influenciadas pelas estratégias de resolução de problemas utilizadas pelos alunos? Que papéis têm os diferentes tipos de representação na resolução dos problemas? Nesta investigação assume-se que a resolução de problemas constitui uma actividade muito importante na aprendizagem da Matemática no 1.o Ciclo do Ensino Básico. Os problemas devem ser variados, apelar a estratégias diversificadas de resolução e permitir diferentes representações por parte dos alunos. As representações cativas, icónicas e simbólicas constituem importantes ferramentas para os alunos organizarem, registarem e comunicarem as suas ideias matemáticas, nomeadamente no âmbito da resolução de problemas, servindo igualmente de apoio à compreensão de conceitos e relações matemáticas. A metodologia de investigação segue uma abordagem interpretativa tomando por design o estudo de caso. Trata-se simultaneamente de uma investigação sobre a própria prática, correspondendo os quatro estudos de caso a quatro alunos da turma de 1.0 ano de escolaridade da investigadora. A recolha de dados teve lugar durante o ano lectivo 2007/2008 e recorreu à observação, à análise de documentos, a diários, a registos áudio/vídeo e ainda a conversas com os alunos. A análise de dados que, numa primeira fase, acompanhou a recolha de dados, teve como base o problema e as questões da investigação bem como o referencial teórico que serviu de suporte à investigação. Com base no referencial teórico e durante o início do processo de análise, foram definidas as categorias de análise principais, sujeitas posteriormente a um processo de adequação e refinamento no decorrer da análise e tratamento dos dados recolhidos -com vista à construção dos casos em estudo. Os resultados desta investigação apontam as representações do tipo icónico e as do tipo simbólico como as representações preferenciais dos alunos, embora sejam utilizadas de formas diferentes, com funções distintas e em contextos diversos. Os elementos simbólicos apoiam-se frequentemente em elementos icónicos, sendo estes últimos que ajudam os alunos a descompactar o problema e a interpretá-lo. Nas representações icónicas enfatiza-se o papel do diagrama, o qual constitui uma preciosa ferramenta de apoio ao raciocínio matemático. Conclui-se ainda que enquanto as representações activas dão mais apoio a estratégias de resolução que envolvem simulação, as representações icónicas e simbólicas são utilizadas com estratégias diversificadas. As representações construídas, com papéis e funções diferentes entre si, e que desempenham um papel crucial na correcta interpretação e resolução dos problemas, parecem estar directamente relacionadas com as caraterísticas da tarefa proposta no que diz respeito às estruturas matemáticas envolvidas. ABSTRACT; The objective of the present study is to investigate the role of the representations constructed by 1st grade students in mathematical problem solving. More specifically, this research is oriented by the following questions: Which representations are preferably used by students to solve problems? ln which way the strategies adopted by the students in problem solving influence those distinct representations? What is the role of the distinct types of representation in the problems solving process? ln this research it is assumed that the resolution of problems is a very important activity in the Mathematics learning at the first cycle of basic education. The problems must be varied, appealing to diverse strategies of resolution and allow students to construct distinct representations. The active, iconic and symbolic representations are important tools for students to organize, to record and to communicate their mathematical ideas, particularly in problem solving context, as well as supporting the understanding of mathematical concepts and relationships. The adopted research methodology follows an interpretative approach, and was developed in the context of the researcher classroom, originating four case studies corresponding to four 1 st grade students of the researcher's class. Data collection was carried out during the academic year of 2007/2008 and was based on observation, analysis of documents, diaries, audio and video records and informal conversations with students. The initial data analysis was based on the problems and issues of research, as well in the theoretical framework that supports it. The main categories of analysis were defined based on the theoretical framework, and were subjected to a process of adaptation and refining during data processing and analysis aiming the -case studies construction. The results show that student's preferential representations are the iconic and the symbolic, although these types of representations are used in different ways, with different functions and in different contexts. The symbolic elements are often supported by iconic elements, the latter helping students to unpack the problem and interpret it. ln the iconic representations the role of the diagrams is emphasized, consisting in a valuable tool to support the mathematical reasoning. One can also conclude that while the active representations give more support to the resolution strategies involving simulation, the iconic and symbolic representations are preferably used with different strategies. The representations constructed with distinct roles and functions, are crucial in the proper interpretation and resolution of problems, and seem to be directly related to the characteristics of the proposed task with regard to the mathematical structures involved.

Rapport sur le fonctionnement de l'Institut Océanographique de l'Indochine pendant l'année 1935-1936

The present note deals with the functions and activities carried out by the Institute of oceanography of Indochina during 1935-1936

Rapport sur le fonctionnement de l'Institut Océanographique de l'Indochine pendant l'année 1936-1937

The present note deals with the functions and activities carried out by the Institute of oceanography of Indochina during 1936-1937

Rapport sur le fonctionnement de l'Institut Océanographique de l'Indochine pendant l'année 1937-1938

The present note deals with the functions and activities carried out by the Institute of oceanography of Indochina during 1937-1938

Development of dendritic cells in the intestine

The intestinal tract is exposed to a large variety of antigens such as food proteins, commensal bacteria and pathogens and contains one of the largest arms of the immune system. The intestinal immune system has to discriminate between harmless and harmful antigens, inducing tolerance to harmless antigens and active immunity towards pathogens and other harmful materials. Dendritic cells (DC) in the mucosal lamina propria (LP) are central to this process, as they sample bacteria from the local environment and constitutively migrate to the draining mesenteric lymph nodes (MLN), where they present antigen to naïve T cells in order to direct an appropriate immune response. Despite their crucial role, understanding the function and phenotype of LP DC has been hampered by the fact that they share phenotypic markers with macrophages (mφ), which are the dominant population of mononuclear phagocyte (MP) in the LP. Recent work in our own and other laboratories has established gating strategies and phenotyping panels that allow precise discrimination between intestinal DC and mφ using the mφ specific markers CD64 and F4/80. In this way four bona fide DC subsets with distinct functions have been identified in adult LP based on their expression of CD11b and CD103 and a major aim of my project was to understand how these subsets might develop in the neonatal intestine. At the beginning of my PhD, the laboratory had used these new methods to show that signal regulatory protein α (SIRPα), an inhibitory receptor expressed by myeloid cells, was expressed by mφ and most DC in the intestine, except for those expressing CD103 alone. In addition, mice carrying a non-signalling mutation in SIRPα (SIRPα mt) had a selective reduction in CD103+CD11b+ DC, a subset which is unique to the intestinal LP. This was the basis for the initial experiments of my project, described in Chapter 3, where I investigated if the phenotype in SIRPα mt mice was intrinsic to haematopoietic cells or not. To explore this, I generated bone marrow (BM) chimeric mice by reconstituting irradiated WT mice with SIRPα mt BM, or SIRPα mt animals with WT BM. These experiments suggested that the defect in CD103+CD11b+ DC was not replicated in DC derived from BM of SIRPα origin. However as this seemed inconsistent with other data, I considered the possibility that 18 the phenotype may have been lost with age, as the BM chimeric mice were considerably older than those used in the original studies of SIRPα function. However a comparison of DC subsets in the intestine of WT and SIRPα mt mice as they aged provided no conclusive evidence to support this idea. As these experiments did show age-dependent effects on DC subsets, in Chapter 4, I went on to investigate how the DC populations appeared in the intestine and other tissues in the neonatal period. These experiments showed there were few CD103+CD11b+ DC present in the LP and migratory DC compartment of the MLN in the neonate and that as this population gradually increased in proportion with age, there was a reciprocal decrease in the relative proportion of CD103-CD11b+ DC. Interestingly, most of the changes in DC numbers in the intestine were found during the second or third week of life when the weaning process began. To validate my findings that there were few CD103+CD11b+ DC in the neonate and that this was not merely an absence of CD103 upregulation, I examined the expression of CD101 and Trem-1, markers that other work in the laboratory had suggested were specific to the CD103+CD11b+ DC lineage. My work showed that CD101 and Trem-1 were co- expressed by most CD103+CD11b+ DC in small intestine (SI) LP, as well as a small subset of CD103-CD11b+ DC in this tissue. Interestingly, Trem-1 was highly specific to the SI LP and migratory DC in the MLN, but absent from the colon and other tissues. CD101 expression was also only found on CD11b+ DC, but showed a less restricted pattern of distribution, being found in several tissues as well as the SI LP. The relative timing of their development suggested there might be a relationship between CD103+CD11b+ and CD103-CD11b+ DC and this was supported by microarray analysis. I hypothesised that the CD103-CD11b+ DC that co-expressed CD101 and Trem-1 may be the cells that developed into CD103+CD11b+ DC. To investigate this I analysed how CD101 and Trem-1 expression changed with age amongst the DC subsets in SI LP, colonic LP (CLP) and MLN. The proportion of CD101+Trem-1+ cells increased amongst CD103+CD11b+ DC in the SI LP and MLN with age, while amongst CD103+CD11b+ DC in the CLP this decreased. This was not the same in CD103-CD11b+ DC, where CD101 and Trem-1 expression was more varied with age in all tissues. CD101 and Trem-1 were not expressed to any great extent on CD103+CD11b- or CD103-CD11b- DC. The phenotypic development of the 19 intestinal DC subsets was paralleled by the gradual upregulation of CD103 expression, while the production of retinoic acid (RA), as assessed by the AldefluorTM assay, was low early in life and did not attain adult levels until after weaning. Thus DC in the neonatal intestine take some time to acquire the adult pattern of phenotypic subsets and are functionally immature compared with their adult counterparts. In Chapter 5, I used CD101 and Trem-1 to explore the ontogeny of intestinal DC subsets in CCR2-/- and SIRPα mt mice, both of which have selective defects in one particular group of DC. The selective defect seen amongst CD103+CD11b+ DC in adult SIRPα mt mice was more profound in mice at D7 and D14 of age, indicating that it may be intrinsic to this population and not highly dependent on environmental factors that change after birth. The expression of CD101 and Trem-1 by both CD103+CD11b+ and CD103-CD11b+ DC was reduced in SIRPα mt mice, again indicating that this entire lineage was affected by the lack of SIRPα signalling. However there was also a generalised defect in the numbers of all DC subsets in many tissues from early in life, suggesting there was compromised development, recruitment or survival of DC in the absence of SIRPα signalling. In contrast to the findings in SIRPα mt mice, more CD103+CD11b+ DC co-expressed CD101 and Trem-1 in CCR2-/- mice, while there were no differences in the expression of these molecules amongst CD103-CD11b+ DC. This may suggest that CCR2+ CD103-CD11b+ DC are not the cells that express CD101 and Trem-1 that are predicted to be the direct precursors of CD103+CD11b+ DC. I also examined the expression of DC growth factor receptors on DC subsets from mice of different ages, but no clear age or subset- related patterns of the expression of mRNA for Csf2ra, Irf4, Tgfbr1 and Rara could be observed. Next, I investigated whether Trem-1 played any role in DC development. Preliminary experiments in Trem-1-/- mice show no differences between any of the DC subsets, nor were there any selective effects on individual subsets when DC development from Trem-1-/- KO and WT BM was compared in competitive chimeras. However these experiments were difficult to interpret due to viability problems and because I found an unexpected defect in the ability of Trem-1-/- BM to generate all DC, irrespective of whether they expressed Trem-1 or not. 20 The final experiments I carried out were to examine the role of the microbiota in driving the differentiation of intestinal DC subsets, based on the hypothesis that this could be one of the environmental factors that might influence events in the developing intestine. To this end I performed experiments in both antibiotic treated and germ free adult mice, both of which showed no significant phenotypic differences amongst any of the DC subsets. However the study of germ free mice was compromised by recent contamination of the colony and may not be the conclusive answer. Together the data in this thesis have shown that the population of CD103+CD11b+ DC, which is unique to the intestine, is not present at birth. These cells gradually increase in frequency over time and as this occurs there is a reciprocal decrease in the frequency of CD103-CD11b+ DC. Along with other results, this leads to the idea that there may be a linear developmental pathway from CD103-CD11b+ DC to CD103+CD11b+ DC that is driven by non-microbial factors that are located preferentially in the small intestine. My project indicates that markers such as CD101 and Trem-1 may assist the dissection of this process and highlights the importance of the neonatal period for these events.

Rapport sur le fonctionnement de l'Institut Océanographique de l'Indochine pendant l'années 1938-1947

The present note deals with the functions and activities done by the Institute of oceanography of Indochina during 1938-1947.

RestrictionDigest: A powerful Perl module for simulating genomic restriction digests

Background: Reduced-representation sequencing technology iswidely used in genotyping for its economical and efficient features. A popular way to construct the reduced-representation sequencing libraries is to digest the genomic DNA with restriction enzymes. A key factor of this method is to determine the restriction enzyme(s). But there are few computer programs which can evaluate the usability of restriction enzymes in reduced-representation sequencing. SimRAD is an R package which can simulate the digestion of DNA sequence by restriction enzymes and return enzyme loci number as well as fragment number. But for linkage mapping analysis, enzyme loci distribution is also an important factor to evaluate the enzyme. For phylogenetic studies, comparison of the enzyme performance across multiple genomes is important. It is strongly needed to develop a simulation tool to implement these functions. Results: Here, we introduce a Perl module named RestrictionDigest with more functions and improved performance. It can analyze multiple genomes at one run and generate concise comparison of enzyme performance across the genomes. It can simulate single-enzyme digestion, double-enzyme digestion and size selection process and generate comprehensive information of the simulation including enzyme loci number, fragment number, sequences of the fragments, positions of restriction sites on the genome, the coverage of digested fragments on different genome regions and detailed fragment length distribution. Conclusions: RestrictionDigest is an easy-to-use Perl module with flexible parameter settings.With the help of the information produced by the module, researchers can easily determine the most appropriate enzymes to construct the reduced-representation libraries to meet their experimental requirements.