A microarray study of post-mortem mRNA degradation in mouse brain tissue

Background: Although there is evidence that post-mortem interval (PMI) is not a major contributor to reduced overall RNA integrity, it may differentially affect a subgroup of gene transcripts that are susceptible to PMI-related degradation. This would particularly have ramifications for microarray studies that include a broad spectrum of genes. Method: Brain tissue was removed from adult mice at 0, 6, 12, 18, 24,36 and 48 h post-mortem. RNA transcript abundance was measured by hybridising RNA from the zero time point with test RNA from each PMI time point, and differential gene expression was assessed using cDNA microarrays. Sequence and ontological analyses were performed on the group of RNA transcripts showing greater than two-fold reduction. Results: Increasing PMI was associated with decreased tissue pH and increased RNA degradation as indexed by 28S/18S ribosomal RNA ratio. Approximately 12% of mRNAs detected on the arrays displayed more than a two-fold decrease in abundance by 48 It post-mortem. An analysis of nucleotide composition provided evidence that transcripts with the AUUUA motif in the 3' untranslated region (3'UTR) were more susceptible to PMI-related RNA degradation, compared to transcripts not carrying the 3'UTR AUUUA motif. Consistent with this finding, ontological analysis showed transcription factors and elements to be over-represented in the group of transcripts susceptible to degradation. Conclusion: A subgroup of mammalian mRNA transcripts are particularly susceptible to PMI-related degradation, and as a group, they are more likely to carry the YUTR AUUUA motif. PMI should be controlled for in human and animal model post-mortem brain studies, particularly those including a broad spectrum of mRNA transcripts. (c) 2005 Elsevier B.V. All rights reserved.

Temporal and spatial transcriptional programs in murine kidney development

We have performed a systematic temporal and spatial expression profiling of the developing mouse kidney using Compugen long-oligonucleotide microarrays. The activity of 18,000 genes was monitored at 24-h intervals from 10.5-day-postcoitum (dpc) metanephric mesenchyme (MM) through to neonatal kidney, and a cohort of 3,600 dynamically expressed genes was identified. Early metanephric development was further surveyed by directly comparing RNA from 10.5 vs. 11.5 vs. 13.5dpc kidneys. These data showed high concordance with the previously published dynamic profile of rat kidney development (Stuart RO, Bush KT, and Nigam SK. Proc Natl Acad Sci USA 98: 5649-5654, 2001) and our own temporal data. Cluster analyses were used to identify gene ontological terms, functional annotations, and pathways associated with temporal expression profiles. Genetic network analysis was also used to identify biological networks that have maximal transcriptional activity during early metanephric development, highlighting the involvement of proliferation and differentiation. Differential gene expression was validated using whole mount and section in situ hybridization of staged embryonic kidneys. Two spatial profiling experiments were also undertaken. MM (10.5dpc) was compared with adjacent intermediate mesenchyme to further define metanephric commitment. To define the genes involved in branching and in the induction of nephrogenesis, expression profiling was performed on ureteric bud (GFP+) FACS sorted from HoxB7-GFP transgenic mice at 15.5dpc vs. the GFP- mesenchymal derivatives. Comparisons between temporal and spatial data enhanced the ability to predict function for genes and networks. This study provides the most comprehensive temporal and spatial survey of kidney development to date, and the compilation of these transcriptional surveys provides important insights into metanephric development that can now be functionally tested.

Using BWW model to evaluate building ontologies in CGs formalism

The Bunge-Wand-Weber (BWW) representation model defines ontological constructs for information systems. According to these constructs the completeness and efficiency of a modeling technique can be defined. Ontology plays an essential role in e-commerce. Using or updating an existing ontology and providing tools to solve any semantic conflicts become essential steps before putting a system online. We use conceptual graphs (CGs) to implement ontologies. This paper evaluates CG capabilities using the BWW representation model. It finds out that CGs are ontologically complete according to Wand and Weber definition. Also it finds out that CGs have construct overload and construct redundancy which can undermine the ontological clarity of CGs. This leads us to build a meta-model to avoid some ontological-unclarity problems. We use some of the BWW constructs to build the meta-model. (c) 2004 Elsevier Ltd. All rights reserved.

Blocking the path from vagueness to four dimensionalism

There is a general form of an argument which I call the 'argument from vagueness' which attempts to show that objects persist by perduring, via the claim that vagueness is never ontological in nature and thus that composition is unrestricted. I argue that even if we grant that vagueness is always the result of semantic indeterminacy rather than ontological vagueness, and thus also grant that composition is unrestricted, it does not follow that objects persist by perduring. Unrestricted mereological composition lacks the power to ensure that there exist instantaneous objects that wholly overlap persisting objects at times, and thus lacks the power to ensure that there exists anything that could be called a temporal part. Even if we grant that such instantaneous objects exist, however, I argue that it does not follow that objects perdure. To show this I briefly outline a coherent version of three dimensionalism that grants just such an assumption. Thus considerations pertaining to the nature of vagueness need not lead us inevitably to accept perdurantism.

Postcolonialism and the politics of qualitative research in international business

This paper offers a re-configuration of international business research by subjecting it to a postcolonial critique. This critique sees international business research as exhibiting continuities with the colonial project in the way it appropriates the Other. Qualitative research in international business often reproduces a neopositivist separation of theory and method, which can marginalize discussion of the important ontological, epistemological and political issues inherent in any research process

Finding common ground in team-based qualitative research using the convergent interviewing method

Research councils, agencies, and researchers recognize the benefits of team-based health research. However, researchers involved in large-scale team-based research projects face multiple challenges as they seek to identify epistemological and ontological common ground. Typically, these challenges occur between quantitative and qualitative researchers but can occur between qualitative researchers, particularly when the project involves multiple disciplinary perspectives. The authors use the convergent interviewing technique in their multidisciplinary research project to overcome these challenges. This technique assists them in developing common epistemological and ontological ground while enabling swift and detailed data collection and analysis. Although convergent interviewing is a relatively new method described primarily in marketing research, it compares and contrasts well with grounded theory and other techniques. The authors argue that this process provides a rigorous method to structure and refine research projects and requires researchers to identify and be accountable for developing a common epistemological and ontological position.

L'aperception de soi chez shihab al-din al-suhrawardi et l'heritage avicennien

Avicenna (d. 1037) bequeathed the Arabic philosophical tradition with an aporia : self-knowledge is conceived, at times, in terms of intellection, at other times, in terms of apperception. In his Book of Discussions and Book of Notes, Avicenna has lengthy discussions on apperception, defined as a direct ontological mode of knowledge. Heir to this tradition, Shihāb al-Dīn al-Suhrawardī (d. 1191) moved away from the first conception of self-knowledge as intellection to adopt the second conception of an apperception of the self as a direct, intuitive and "presential (hcombining dot belowudcombining dot belowūrī)" perception, and which he defended with four types of arguments.

Levinas and the defination of philosophy: An ethical approach

Emmanuel Levinas’ thought seems to be strictly neither rational, phenomenological nor ontological, and it thus intentionally exposes itself to the asking of the question ‘why call it philosophy at all’? While we may have trouble containing Levinas’ thought within our traditional philosophical boundaries, I argue that this gives us no reason to exclude him from philosophy proper as a mere poser, but rather provides the occasion for reflection on just what it means, in an ethical manner, to call something ‘philosophical’. Instead of asking whether or not philosophy can ‘contain’ Levinas’ thought, I contend that it would be more ethical to instead re-phrase the question in terms of ‘sociality’. When we do this, I argue, we can indeed justifiably call Levinas’ thought philosophy.

Levinasian ethics and the representation of the other in international and cross-cultural management

In this paper, we seek to further the discussion, problematization and critique of west/east identity relations in ICM studies by considering the ethics of the relationship – an issue never far beneath the surface in discussions of Orientalism. In particular we seek to both examine and question the ethics of representation in relation to a critique of what has come to be known as international and cross-cultural management (ICM). To pursue such a discussion, we draw specifically on the ethical elaborations of Emmanuel Levinas as well as his chief interlocutors Jacques Derrida and Zygmunt Bauman. The value of this discussion, we propose, is that Levinas offers a philosophy that holds as its central concept the relationship between the self and Other as the primary ethical and pre-ontological relation. Levinas’ philosophy provides a means of extending the post-colonial critique of ICM, and ICM provides a context in which the Levinasian ethics can be brought to bear on a significant issue on contemporary business and management.

Representing part-whole relationships in conceptual modeling: An empirical evaluation

The part-of or part-whole construct is a fundamental element of many conceptual modeling grammars that is used to associate one thing (a component) with another thing (a composite). Substantive theoretical issues surrounding the part-whole construct remain to be resolved, however. For instance, contrary to widespread claims, the relationship between components and composites is not always transitive. Moreover, how the partwhole construct should be represented in a conceptual schema diagram remains a contentious issue. Some analysts argue composites should be represented as a relationship or association. Others argue they should be represented as an entity. In this paper we use an ontological theory to support our arguments that composites should be represented as entities and not relationships or associations. We also describe an experiment that we undertook to test whether representing composites as relationships or entities enables users to understand a domain better. Our results support our arguments that using entities to represent composites enables users to better understand a domain.

OntoRama: Browsing RDF ontologies using a hyperbolic-style browser

This paper presents a Java-based hyperbolic-style browser designed to render RDF files as structured ontological maps. The program was motivated by the need to browse the content of a web-accessible ontology server: WEB KB-2. The ontology server contains descriptions of over 74,500 object types derived from the WordNet 1.7 lexical database and can be accessed using RDF syntax. Such a structure creates complications for hyperbolic-style displays. In WEB KB-2 there are 140 stable ontology link types and a hyperbolic display needs to filter and iconify the view so different link relations can be distinguished in multi-link views. Our browsing tool, OntoRama, is therefore motivated by two possibly interfering aims: the first to display up to 10 times the number of nodes in a hyperbolic-style view than using a conventional graphics display; secondly, to render the ontology with multiple links comprehensible in that view.

New descriptions and understandings of internationalisation: A tale of knowledge-intensive SMEs

This research seeks to generate and foster new descriptions and understandings of processes underlying the internationalisation experienced by small- and medium-sized, knowledge-intensive enterprises. The longitudinal study centres on the growth and internationalisation of a cluster of small- and medium-sized enterprises (SMEs) in the most southern state of Australia, of which a number were 'bom global.' It draws on both retrospective data such as corporate archives, as well as observations and interviews as events unfolded over a period of eighteen months to garner insights into processes underlying the SMEs' internationalisation. The approach to inquiry is influenced by an epistemology of social constructionism, interpretive narrative, sensemaking and dramaturgical theoretical perspectives, and elements of cultural anthropology. Exploratory in the early stages, a funnel approach characteristic of ethnographic enquiry was used whereby the study became progressively focused over time. The extended period of fieldwork led to observations and interpretations that cast the retrospective data in new light, and the use of the construct 'legitimacy' as a lens through which to view activities and events infusing the firms' internationalisation. A generic narrative scheme that offers a temporal ordering of actions, context and meaning attributions in relation to legitimation behaviours and internationalisation processes is developed. This narrative scheme is then used to garner a deeper understanding of three activities that were central to the firms' internationalisation over time: the choice of geographic export markets, strategic participation in international standard-setting committees, and portfolio entrepreneurship. In addition, the study offers a rich story of the growth and internationalisation of the cluster of knowledge-intensive SMEs. The tale of growth and internationalisation pursued by the cluster of knowledgeintensive SMEs spans the period from 1975 to mid 1997, and may prove a useful resource for the theorising of others.

ANTROPOLOGIA TRANSCENDENTAL E CORRELAÇÃO : KARL RAHNER E PAUL TILLICH EM DIALOGO - UMA LEITURA INTERCONFESSIONAL DE MÉTODOS TEOLÓGICOS A PARTIR DA ONTOLOGIA EXISTENCIAL DE MARTIN HEIDEGGER

Esta tese de Doutorado procura estudar os métodos teológicos em diálogo, antropologiatranscendental de Karl Rahner e correlação de Paul Tillich, a partir da sistematização da ontologia existencial elaborada pelo jovem Heidegger em Sein und Zeit. Tanto num, como noutro método, o que se discute é a profundidade do ser, na sua possibilidade de aproximação em superação ao que não pode ser dito. Nesse caso, tanto a metafísica tomista, resgatada por Rahner, quanto a secularização protestante e o abismo do ser, enfatizado por Tillich, assumem a impossibilidade de se dizer o conteúdo do sagrado, exatamente por se situarem ou no inconceito da raiz ontológica (Rahner), ou no excesso de sentido do Ultimate Concern (Tillich). Dessas impossibilades, independente se um antes e depois (Rahner), ou se um depois e um antes em profundidade (Tillich), o que se tem é a pergunta ontológica como possibilidade da abertura do ser, nos dois casos sem conteúdo, para que a resposta , que também não responde, seja dada como (im)possibilidade do deslocamento do ser. No resgate da metafísica tomista, em Rahner, há um deslocamento a partir de um sentido, de certo modo linear , daí antropologia-transcendental. Já na leitura protestante de Tillich, o deslocamento se dá por uma dialética , o ser ameaçado pelo não-ser, como uma unidade rompida, daí correlação. A tese é apresentada em quatro capítulos. No primeiro há uma leitura de Heidegger, pela perspectiva da hermenêutica da compreensão. Já no segundo a reflexão se dá em busca da possibilidade da compreensão pela racionalidade ontológica na correlação, Paul Tillich, pela mediação do simbólico. No terceiro, seguindo as mesmas preocupações, se faz a leitura da epistemologia na racionalidade ontológica na antropologia -transcendental, Karl Rahner, pela mediação da pré-apreensão. O quarto capítulo apresenta temas decorrentes, e tradicionais da teologia, em diálogo a partir dos métodos.(AU)

ANÁLISE DA EPISTEMOLOGIA-ONTOLÓGICA DE PAUL TILLICH: O Princípio de Identidade e implicações para o método e saber teológico

A pesquisa analisa e epistemologia ontológica de Paul Tillich. Considerando a influência de F. W. J. Schelling pretende-se destacar o lugar de certas premissas ontológicas que fundamentaram o pensamento de Tillich, principalmente o Princípio de Identidade , como um dos princípios epistemológicos chave. Partindo do pressuposto de que sua ontologia depende do Princípio de identidade a fim de que possa relacionar essência e existência, destaca-se a identidade substancial entre teologia e filosofia, permitindo melhor compreensão da relação entre ontologia e teologia, caracterizando de forma geral tanto a noção teológicofilosófica da experiência de finitude - choque ontológico como sua relação com as importantes noções de Deus como o Ser-em-si, e ultimate concern enquanto categoria ontológico-teológica. A seguir, críticas voltadas para sua construtividade ontológica serão destacadas e analisadas em termos de seus pressupostos e em seu poder de alcance. Neste âmbito crítico, considerar-se-á breve comparação entre a tentativa do sistema ontológicoteológico de Tillich e os sistemas de Kant e Hegel, com o objetivo de apresentar paralelos críticos entre a postura de tais sistemas no campo epistemológico e ontológico, e suas influências sobre a teologia. Ao final, pretende-se entender as implicações de sua ontologiateológica tanto para a forma metodológica de correlação entre situação e resposta, como na relação com outros sistemas teológicos, que possibilitaram ou não, mediações entre cultura e teologia. Neste segmento, notar-se-á como uma das implicações da ontologia-teológica de Tillich, o imprescindível retorno do saber teológico entre os demais saberes delineadores da realidade; considerando-se certas possibilidades críticas a partir de seu pensamento com respeito ao uso da razão instrumental, ética e espiritualidade.

GESTÃO DO CONHECIMENTO: UM ESTUDO SOBRE A RELAÇÃO ENTRE A LIDERANÇA TRANSFORMACIONAL, A CONVERSÃO DO CONHECIMENTO E A EFICÁCIA ORGANIZACIONAL

Este estudo teve como objetivo principal analisar a relação entre a Liderança Transformacional, a Conversão do Conhecimento e a Eficácia Organizacional. Foram considerados como pressupostos teóricos conceitos consolidados sobre os temas desta relação, além de recentes pesquisas já realizadas em outros países e contextos organizacionais. Com base nisto identificou-se potencial estudo de um modelo que relacionasse estes três conceitos. Para tal considera-se que as organizações que buscam atingir Vantagem Competitiva e incorporam a Knowledge-Based View possam conquistar diferenciação frente a seus concorrentes. Nesse contexto o conhecimento ganha maior destaque e papel protagonista nestas organizações. Dessa forma criar conhecimento através de seus colaboradores, passa a ser um dos desafios dessas organizações ao passo que sugere melhoria de seus indicadores Econômicos, Sociais, Sistêmicos e Políticos, o que se define por Eficácia Organizacional. Portanto os modos de conversão do conhecimento nas organizações, demonstram relevância, uma vez que se cria e se converte conhecimentos através da interação entre o conhecimento existente de seus colaboradores. Essa conversão do conhecimento ou modelo SECI possui quatro modos que são a Socialização, Externalização, Combinação e Internalização. Nessa perspectiva a liderança nas organizações apresenta-se como um elemento capaz de influenciar seus colaboradores, propiciando maior dinâmica ao modelo SECI de conversão do conhecimento. Se identifica então na liderança do tipo Transformacional, características que possam influenciar colaboradores e entende-se que esta relação entre a Liderança Transformacional e a Conversão do Conhecimento possa ter influência positiva nos indicadores da Eficácia Organizacional. Dessa forma esta pesquisa buscou analisar um modelo que explorasse essa relação entre a liderança do tipo Transformacional, a Conversão do Conhecimento (SECI) e a Eficácia Organizacional. Esta pesquisa teve o caráter quantitativo com coleta de dados através do método survey, obtendo um total de 230 respondentes válidos de diferentes organizações. O instrumento de coleta de dados foi composto por afirmativas relativas ao modelo de relação pesquisado com um total de 44 itens. O perfil de respondentes concentrou-se entre 30 e 39 anos de idade, com a predominância de organizações privadas e de departamentos de TI/Telecom, Docência e Recursos Humanos respectivamente. O tratamento dos dados foi através da Análise Fatorial Exploratória e Modelagem de Equações Estruturais via Partial Least Square Path Modeling (PLS-PM). Como resultado da análise desta pesquisa, as hipóteses puderam ser confirmadas, concluindo que a Liderança Transformacional apresenta influência positiva nos modos de Conversão do Conhecimento e que; a Conversão do Conhecimento influencia positivamente na Eficácia Organizacional. Ainda, concluiu-se que a percepção entre os respondentes não apresenta resultado diferente sobre o modelo desta pesquisa entre quem possui ou não função de liderança.