New Building - Interior - Composing Room - Wide Angle (Best Picture) - 8 x 11 Negative [Detroit News]

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A report of the State-wide highway planning survey, Project no. SWHP-1. Summary report, State-wide highway planning survey.

Mode of access: Internet.

National housing act : hearings before the Committee on Banking and Currency, United States Senate, Seventy-third Congress, second session, on S. 3603, a bill to improve nation-wide housing standards, provide employment and stimulate industry ; to improve conditions with respect to home mortgage investment, and to eliminate the necessity for costly second-mortgage financing, by creating a system of mutual mortgage insurance and by making provision for the organization of additional institutions to handle home financing ; to promote thrift and protect savings ; to amend the Federal home loan bank act ; to amend the Federal reserve act ; and for other purposes. May 16-24, 1934.

Duncan U. Fletcher, chairman.

The Wide World Magazine

Mode of access: Internet.

Motorist aid citizens radio service as a wide area communication system /

Mode of access: Internet.

Alexander Legge, 1866-1933; the life story of a truly great American who loved and served his country well, who achieved world-wide distinction as an industrial leader, as a patriotic public servant and as the devoted friend and champion of all who till the soil,

Mode of access: Internet.

Police allocation manual user's guide : determination of the number and allocation of personnel for police traffic services for state-wide agencies /

"DOT HS 807 793"--P. [4] of cover.

The wide Northwest; historic narrative of America's wonder land as seen by a pioneer teacher, Leoti L. West.

Mode of access: Internet.

Wide fields,

Mode of access: Internet.

Method for generation of homogeneous multicellular tumor spheroids applicable to a wide variety of cell types

Multicellular tumor spheroids (MCTS) are used as organotypic models of normal and solid tumor tissue. Traditional techniques for generating MCTS, such as growth on nonadherent surfaces, in suspension, or on scaffolds, have a number of drawbacks, including the need for manual selection to achieve a homogeneous population and the use of nonphysiological matrix compounds. In this study we describe a mild method for the generation of MCTS, in which individual spheroids form in hanging drops suspended from a microtiter plate. The method has been successfully applied to a broad range of cell lines and shows nearly 100% efficiency (i.e., one spheroid per drop). Using the hepatoma cell line, HepG2, the hanging drop method generated well-rounded MCTS with a narrow size distribution (coefficient of variation [CV] 10% to 15%, compared with 40% to 60% for growth on nonadherent surfaces). Structural analysis of HepG2 and a mammary gland adenocarcinoma cell line, MCF-7, composed spheroids, revealed highly organized, three-dimensional, tissue-like structures with an extensive extracellular matrix. The hanging drop method represents an attractive alternative for MCTS production, because it is mild, can be applied to a wide variety of cell lines, and can produce spheroids of a homogeneous size without the need for sieving or manual selection. The method has applications for basic studies of physiology and metabolism, tumor biology, toxicology, cellular organization, and the development of bioartificial tissue. (C) 2003 Wiley Periodicals, Inc.

The changing public-private infrastructure mix: Economy-wide implications

This article provides an economy-wide perspective on the changing role of the public sector in developing economic and social infrastructure in Australia. It analyses the scale and macroeconomic significance of the key economic and social infrastructure sectors - communication services, electricity, gas and water supply, transport, education, health and community services, government administration and defence. It then canvasses the major policy issues that have arisen in the progression from public to private infrastructure provision and considers why concerns about the trend fall in traditional public works spending may be misplaced in light of recent economic and institutional changes.