Electrooptic characterization of tunable cylindrical liquid crystal lenses

In this work, one-dimensional arrays of cylindrical adaptive liquid crystal lenses were manufactured and characterized; and test devices were filled with nematic liquid crystal. Comb interdigitated electrodes were designed as a mask pattern for the control electrode on the top glass substrates. A radial graded refractive index along each microsized lens was achieved by fabricating a layer of high resistance sheet deposited as a control electrode. These tunable lenses were switched by applying amplitude and frequency optimized waveforms on the control electrode. Phase profiles generated by the radial electric field distribution on each lens were measured by a convectional interferometric technique.

Lenticular arrays based on liquid crystals

Lenticular array products have experienced a growing interest in the last decade due to the very wide range of applications they can cover. Indeed, this kind of lenses can create different effects on a viewing image such as 3D, flips, zoom, etc. In this sense, lenticular based on liquid crystals (LC) technology is being developed with the aim of tuning the lens profiles simply by controlling the birefringence electrically. In this work, a LC lenticular lens array has been proposed to mimic a GRIN lenticular lens array but adding the capability of tuning their lens profiles. Comb control electrodes have been designed as pattern masks for the ITO on the upper substrate. Suitable high resistivity layers have been chosen to be deposited on the control electrode generating an electric field gradient between teeth of the same electrode. Test measurements have allowed us to demonstrate that values of phase retardations and focal lengths, for an optimal driving waveform, are fairly in agreement. In addition, results of focusing power of tuneable lenses were compared to those of conventional lenses. The behaviour of both kinds of lenses has revealed to be mutually similar for focusing collimated light and for refracting images.

Acoustic characterisation of cinema screens

As a consequence of cinema screens being placed in front of screen-speakers, a reduction in sound quality has been noticed. Cinema screens not only let the sound go through them, but also absorb a small amount of it and reflect the sound which impacts on the screen to the back, coming forward again in case it impacts on the loudspeaker. This backwards reflection in addition to the signal coming from the loudspeaker can lead to constructive or destructive interference at certain frequencies which usually results in comb filtering. In this project, this effect has been studied through researching amongst various data sheet provided by different manufacturers, acoustical measurements completed in the large anechoic chamber of the ISVR and some theoretical models developed with MatLab software. If results obtained with MatLab are accurate enough in comparison to the real measurements taken in the anechoic chamber this would lead to a good way to predict which would be the attenuation added to the system at each frequency, given that not all manufacturers provide an attenuation curve, but only an average attenuation. This average attenuation might be useless as sound waves have different wavelengths and its propagation through partitions varies. In fact, sound is composed by high and low frequencies, where high frequencies are characterised by a small wavelength which is usually easier to attenuate than low frequencies that characterised by bigger wavelengths. Furthermore, this information would be of great value to both screen manufacturers, who could offer a much more precise data in their data sheets; and customers, who would have a great amount of information to their disposal before purchasing and installing anything in their cinemas, being able to know by themselves which screen or loudspeaker should be best to meet their expectative. RESUMEN. La aparición de la digitalización de las bandas sonoras para las películas hace posible la mejora en la calidad de sonido de los cines. Sin embargo, un aspecto a tener en cuenta en esta calidad del sonido es la transmisión de éste a través de la pantalla, ya que normalmente tras ella se encuentran situados los altavoces. Las propiedades acústicas varían dependiendo del tipo de pantalla que se utilice, además de haber poca información a la que acceder para poder valorar su comportamiento. A lo largo de este proyecto, se analizan tres muestras de pantallas distintas donadas por distintos fabricantes para poder llegar a la conclusión de dependiendo del tipo de pantalla cuál es la distancia óptima a la que localizar la pantalla respecto al altavoz y con qué inclinación. Dicho análisis se realizó en la cámara anecoica del ISVR (University of Southampton) mediante la construcción de un marco de madera de 2x2 m en el que tensar las pantallas de cine, y un altavoz cuyo comportamiento sea el más similar al de los altavoces de pantalla reales. Los datos se captaron mediante cuatro micrófonos colocados en posiciones distintas y conectados al software Pulse de Brüel & Kjær, a través del cual se obtuvieron las respuestas en frecuencia del altavoz sin pantalla y con ella a diferentes distancias del altavoz. Posteriormente, los datos se analizaron con MatLab donde se calculó la atenuación, el factor de transmisión de la presión (PTF) y el análisis cepstrum. Finalmente, se realizó un modelo teórico del comportamiento de las pantallas perforadas basado en las placas perforadas utilizadas para atenuar el sonido entre distintas habitaciones. Como conclusión se llegó a que las pantallas curvadas son acústicamente más transparentes que las pantallas perforadas que a partir de 6 kHz son más acústicamente opacas. En las pantallas perforadas la atenuación depende del número de perforaciones por unidad de área y el diámetro de éstas. Dicha atenuación se reducirá si se reduce el diámetro de las perforaciones de la pantalla, o si se incrementa la cantidad de perforaciones. Acerca del efecto filtro peine, para obtener la mínima amplitud de éste la pantalla se deberá situar a una distancia entre 15 y 30 cm del altavoz, encontrando a la distancia de 30 cm que la última reflexión analizada a través de Cepstrum llega 5 ms más tarde que la señal directa, por lo cual no debería dañar el sonido ni la claridad del habla.

Asphalt rubber mixtures with warm mix asphalt additives

En los últimos años, debido a la creciente preocupación por el calentamiento global y el cambio climático, uno de los retos más importantes a los que se enfrenta nuestra sociedad es el uso eficiente y económico de energía así como la necesidad correspondiente de reducir los gases de efecto invernadero (GEI). Las tecnologías de mezclas semicalientes se han convertido en un nuevo e importante tema de investigación en el campo de los materiales para pavimentos ya que ofrece una solución potencial para la reducción del consumo energético y las emisiones de GEI durante la producción y puesta en obra de las mezclas bituminosas. Por otro lado, los pavimentos que contienen polvo de caucho procedente de neumático fuera de uso, al hacer uso productos de desecho, ahorran energía y recursos naturales. Estos pavimentos ofrecen una resistencia mejorada a la formación de roderas, a la fatiga y a la fisuración térmica, reducen los costes de mantenimiento y el ruido del tráfico así como prolongan la vida útil del pavimento. Sin embargo, estas mezclas presentan un importante inconveniente: la temperatura de fabricación se debe aumentar en comparación con las mezclas asfálticas convencionales, ya que la incorporación de caucho aumenta la viscosidad del ligante y, por lo tanto, se producen mayores cantidades de emisiones de GEI. En la presente Tesis, la tecnología de mezclas semicalientes con aditivos orgánicos (Sasobit, Asphaltan A, Asphaltan B, Licomont) se incorporó a la de betunes de alta viscosidad modificados con caucho (15% y 20% de caucho) con la finalidad de dar una solución a los inconvenientes de mezclas con caucho gracias a la utilización de aditivos reductores de la viscosidad. Para este fin, se estudió si sería posible obtener una producción más sostenible de mezclas con betunes de alto contenido en caucho sin afectar significativamente su nivel de rendimiento mecánico. La metodología aplicada para evaluar y comparar las características de las mezclas consistió en la realización de una serie de ensayos de laboratorio para betunes y mezclas con caucho y con aditivos de mezclas semicalientes y de un análisis del ciclo de vida híbrido de la producción de mezclas semicalientes teniendo en cuenta la papel del aditivo en la cadena de suministro con el fin de cuantificar con precisión los beneficios de esta tecnología. Los resultados del estudio indicaron que la incorporación de los aditivos permite reducir la viscosidad de los ligantes y, en consecuencia, las temperaturas de producción y de compactación de las mezclas. Por otro lado, aunque la adición de caucho mejoró significativamente el comportamiento mecánico de los ligantes a baja temperatura reduciendo la susceptibilidad al fenómeno de fisuración térmica, la adición de las ceras aumentó ligeramente la rigidez. Los resultados del estudio reológico mostraron que la adición de porcentajes crecientes de caucho mejoraban la resistencia del pavimento con respecto a la resistencia a la deformación permanente a altas temperaturas y a la fisuración térmica a bajas temperaturas. Además, se observó que los aditivos mejoran la resistencia a roderas y la elasticidad del pavimento al aumentar el módulo complejo a altas temperaturas y al disminuir del ángulo de fase. Por otra parte, el estudio reológico confirmó que los aditivos estudiados aumentan ligeramente la rigidez a bajas temperaturas. Los ensayos de fluencia llevados a cabo con el reómetro demostraron una vez más la mejora en la elasticidad y en la resistencia a la deformación permanente dada por la adición de las ceras. El estudio de mezclas con caucho y aditivos de mezclas semicalientes llevado a cabo demostró que las temperaturas de producción/compactación se pueden disminuir, que las mezclas no experimentarían escurrimiento, que los aditivos no cambian significativamente la resistencia conservada y que cumplen la sensibilidad al agua exigida. Además, los aditivos aumentaron el módulo de rigidez en algunos casos y mejoraron significativamente la resistencia a la deformación permanente. Asimismo, a excepción de uno de los aditivos, las mezclas con ceras tenían la misma o mayor resistencia a la fatiga en comparación con la mezcla control. Los resultados del análisis de ciclo de vida híbrido mostraron que la tecnología de mezclas semicalientes es capaz de ahorrar significativamente energía y reducir las emisiones de GEI, hasta un 18% y 20% respectivamente, en comparación con las mezclas de control. Sin embargo, en algunos de los casos estudiados, debido a la presencia de la cera, la temperatura de fabricación debe reducirse en un promedio de 8 ºC antes de que los beneficios de la reducción de emisiones y el consumo de combustible puedan ser obtenidos. Los principales sectores contribuyentes a los impactos ambientales generados en la fabricación de mezclas semicalientes fueron el sector de los combustibles, el de la minería y el de la construcción. Due to growing concerns over global warming and climate change in recent years, one of the most important challenges facing our society is the efficient and economic use of energy, and with it, the corresponding need to reduce greenhouse gas (GHG) emissions. The Warm Mix Asphalt (WMA) technology has become an important new research topic in the field of pavement materials as it offers a potential solution for the reduction of energy consumption and GHG emissions during the production and placement of asphalt mixtures. On the other hand, pavements containing crumb-rubber modified (CRM) binders save energy and natural resources by making use of waste products. These pavements offer an improved resistance to rutting, fatigue and thermal cracking; reduce traffic noise and maintenance costs and prolong pavement life. These mixtures, however, present one major drawback: the manufacturing temperature is higher compared to conventional asphalt mixtures as the rubber lends greater viscosity to the binder and, therefore, larger amounts of GHG emissions are produced. In this dissertation the WMA technology with organic additives (Sasobit, Asphaltan A, Asphaltan B and Licomont) was applied to CRM binders (15% and 20% of rubber) in order to offer a solution to the drawbacks of asphalt rubber (AR) mixtures thanks to the use of fluidifying additives. For this purpose, this study sought to determine if a more sustainable production of AR mixtures could be obtained without significantly affecting their level of mechanical performance. The methodology applied in order to evaluate and compare the performance of the mixtures consisted of carrying out several laboratory tests for the CRM binders and AR mixtures with WMA additives (AR-WMA mixtures) and a hybrid input-output-based life cycle assessment (hLCA) of the production of WMA. The results of the study indicated that the incorporation of the organic additives were able to reduce the viscosity of the binders and, consequently, the production and compaction temperatures. On the other hand, although the addition of rubber significantly improved the mechanical behaviour of the binders at low temperatures reducing the susceptibility to thermal cracking phenomena, the addition of the waxes slightly increased the stiffness. Master curves showed that the addition of increasing percentages of rubber improved the resistance of the pavement regarding both resistance to permanent deformation at high temperatures and thermal cracking at low temperatures. In addition, the waxes improved the rutting resistance and the elasticity as they increased the complex modulus at high temperatures and decreased the phase angle. Moreover, master curves also attest that the WMA additives studied increase the stiffness at low temperatures. The creep tests carried out proved once again the improvement in the elasticity and in the resistance to permanent deformation given by the addition of the waxes. The AR-WMA mixtures studied have shown that the production/compaction temperatures can be decreased, that the mixtures would not experience binder drainage, that the additives did not significantly change the retained resistance and fulfilled the water sensitivity required. Furthermore, the additives increased the stiffness modulus in some cases and significantly improved the permanent deformation resistance. Except for one of the additives, the waxes had the same or higher fatigue resistance compared to the control mixture. The results of the hLCA demonstrated that the WMA technology is able to significantly save energy and reduce GHG emissions, up to 18% and 20%, respectively, compared to the control mixtures. However, in some of the case studies, due to the presence of wax, the manufacturing temperature at the asphalt plant must be reduced by an average of 8ºC before the benefits of reduced emissions and fuel usage can be obtained. The results regarding the overall impacts generated using a detailed production layer decomposition indicated that fuel, mining and construction sectors are the main contributors to the environmental impacts of manufacturing WMA mixtures.

Influencia del tratamiento "a fuego" en las características del estuco tradicional con cal = Influence of treatment "by fire" in the characteristics of traditional lime stucco

Esta investigación avanza en el intento de recuperar la técnica tradicional del estuco a fuego, paradigma de la decoración de espacios interiores hasta la mitad del s. XX por sus imitaciones marmóreas de gran calidad. Para ejecutarlos se utilizaban morteros de cal con áridos de mármol, pigmentos minerales y grasas; con estos materiales se preparaban y tendían las masas, se pintaban al fresco y se terminaban con el paso de una herramienta metálica caliente. Como sucede con otras técnicas, el paso del tiempo ha difuminado los conocimientos sobre la materia, haciendo muy difícil la conservación o restauración de los ejemplos en nuestro patrimonio. A partir de la escasa bibliografía existente se ha recuperado el proceso, sometiendo las muestras elaboradas a diferentes ensayos que han caracterizado el acabado final,tanto en sus características intrínsecas como en las que le son conferidas al proteger finalmente las muestras con una capa de cera, tal y como se realizaba tradicionalmente.---ABSTRACT---This research advances in the attempt to recover the traditional technique of stucco by fire; paradigm of indoor decoration until half of 20th century due to its high quality marbled imitations. On its implementation process, it was used lime mortars with marble aggregates, mineral pigments and greases. With these materials, masses were prepared and extended into the walls, they were painted in fresco and ended with the passage of a heated metal tool. As with any other technique, the time passing over has blurred the knowledge on the subject, making very difficult the preservation or restoration of examples in our heritage. Despite of the limited literature, the process has been recovered; subjecting the samples prepared at different trials that have characterized the finished, both in its merits and its conferred characteristics by finally protecting the samples with a layer of wax, as was done traditionally.

Liquid crystal temperature sensor based on a micrometric structure and a metallic nanometric layer

This letter presents a novel temperature sensor, which consists of an interdigitated comb electrode structure with a micrometric-scale size, nanometric metallic layer, and nematic liquid crystal (NLC) film. This sensor exploits the permittivity dependence of the NLC with temperature and principle of electrical conductivity above the percolation threshold in thin film metallic layers. The latter has been demonstrated to increase the temperature sensitivity considerably. The high impedance input reduces the power dissipation, and the high enough voltage output makes it easy to measure the output signal with high precision. The operation principle and fabrication process as well as the characterization of the temperature sensor are presented. Experimental results show that the device offers a sensitivity of 9 mV/°C and is dependent on the applied voltage. This is six times greater than the same structure without the use of a nanometric layer.

Cómic, arquitectura narrativa. Describiendo cuatro dimensiones con dos

Cómic, arquitectura narrativa. Describiendo cuatro dimensiones con dos. La mayor parte de los estudios con un enfoque académico en torno a cómic y arquitectura, se han centrado más en las distintas realidades representadas dentro del medio, en sus decorados, que en la estructura del cómic en sí. Existe un aspecto gráfico que se ha venido obviando o al menos no ha adquirido el protagonismo que merece: los espacios representados en el cómic se encuentran en un medio que se concibe y estructura de una manera similar a como el arquitecto dibuja y concibe la arquitectura. En lo básico, históricamente, las herramientas y soportes para idear y representar la arquitectura son muy parecidas a aquellas que usa el autor de cómic. Lápiz, tinta y color sobre un papel, combinadas trabajando en un tablero, ese es lugar de nacimiento de ambos mundos. A lo largo de esta tesis se muestra cómo el dibujo une a creadores de narraciones gráficas y edificios. La relación va más allá de que el cómic pueda utilizar las disposiciones vitruvianas, ichnographia, ortographia o scenographia, (planta, alzado, perspectiva), la propia articulación que hace del espacio de la página, puede sintonizar con la manera en la que se concibe una estructura arquitectónica. La tesis inicia con un capítulo de recorrido en torno a las teorías del cómic existentes, buscando intersecciones posibles con la representación arquitectónica o el proyecto espacial. En este primer paso de estudio del estado de la cuestión han pesado más los trabajos de los últimos veinticinco años. Se trata de un periodo en el que, a la par que se ha producido una explosión creativa en el mundo del cómic, determinados teóricos han incidido en un análisis más formal y visual del medio. Tras recoger teorías en el primer capítulo, se plantea un recorrido histórico a la búsqueda de posibles resonancias de “arquitecturas mentales” en el pasado. Esta búsqueda de referentes más allá del siglo XIX, momento en el que nace el medio en su forma actual, es ya un aspecto comúb en distintos estudios de cómic que han servido de inspiración. Se ha preferido ubicar esta colección de antecedentes como segundo capítulo, evitando dar al trabajo un carácter de historia e incidiendo en su enfoque de tipo visual. El tercer capítulo analiza una serie de obras y autores de cómic en su forma actual, buscando distintas estrategias para la descripción de espacios y las narraciones que ocurren en ellos. El crecimiento de casos en estas últimas décadas ha demostrado hasta qué punto el desarrollo de un campo depende tanto de que exista una masa crítica en un contexto conectado y productivo, como de la aparición de individuos que abren puertas a los demás, con la cristalización personal que hacen de una situación. Se analiza en un cuarto capítulo la relación entre la arquitectura y el cómic modernos. Una relación que se presenta partiendo desde sus respectivos padres, para después tratar un elenco creciente de experiencias en el que la narración gráfica y el hecho arquitectónico estan íntimamente ligados. Inspirado en la estrategia que Gubern y Gasca utilizan en El discurso del cómic (Cátedra, 1988) se cierra la tesis con un discurso visual por las arquitecturas mentales y narrativas del cómic: la representación del espacio a través de juego con las dimensiones, el uso de recursos gráficos propios de la descripción arquitectónica, la sección, la planta, la utilización de elementos constructivos,etc, completando el abanico de estrategias gráficas presentadas. ABSTRACT Comic narrative architecture. Describing four dimensions with two Most of the studies with an academic focus around comics and architecture have centered their view more on the different realities represented in the media, in its sets, than in the structure of comics itself. There is a graphic aspect that has been ignored, or at least has not gained the prominence it deserves: the spaces rendered in the comics are set in a medium that is conceived and structured in a similar way as the architect draws and conceives architecture. In basics, tools for designing and representing architecture are very similar to those used by the cartoonist. Pencil, ink and color on paper, combined working on a board, that is birthplace of both worlds. Throughout this thesis its shown how drawing connects creators of graphic stories and buildings. The relationship goes beyond the use that comics can do of the Vitruvian provisions, ichnographia, ortographia or scenographia (plan, elevation, perspective), to the arrange itself that it makes with space on the page, in tune with the way in which architectural structures are conceived. The thesis starts with a chapter that collects existing comics theories and their possible intersection with architectural rendering. In this first step of study on the state of affairs, the focus has been over studies from the last twenty years. It is a period in which, at the same time that there has been a creative explosion in the world of comics, some theorists have worked with a more formal and visual approach. After collecting theories in the first chapter, a historical tour is made to search for possible resonances of “mental architectures” in the past. This search for references beyond the nineteenth century, when the medium was born in its current form, is already a regular process in various recent studies that have served as inspiration. We preferred to locate this as a second chapter, to avoid giving the work a character of History, and reenforcing its visual approach. The third chapter analyzes a series of comics and authors, looking for different strategies for describing spaces and stories that occur in them. The growth of cases in recent decades has shown how the development of a field depends both on the existence of a critical mass in a connected and productive enviroment, and the appearance of individuals who open doors to others with the personal crystallization that they make on that context. The relationship between architecture and the modern comic is analyzed in a fourth chapter. A relationship that is described starting from their parents, and then going through a growing cast of experiences in which graphic narrative and architecture are closely linked. Inspired by the strategy used Gubern and Gasca in El discurso del cómic (Cátedra, 1988), The Comics’ Speech, the thesis concludes with a visual discourse through this narrative comic architectures: the representation of space by playing with dimensions, the use of resources for architectural rendering, section, plant, etc., to complete the range of graphical strategies presented.

A SENHORA DA BERLINDA Representações sociais do cotidiano na religiosidade popular paraense

O culto a Nossa Senhora de Nazaré é uma devoção popular muito antiga em Belém do Pará, quase tão antiga quanto a cidade, pois esse culto se consolidou na mesma época em que se constituiu o município de Belém e o Estado do Pará. A população de devotos cresceu junto com a população dos habitantes da cidade. Tal fato propiciou que essa devoção popular se enraizasse profundamente na cultura paraense, penetrando até a auto-imagem do paraense católico, moldando a sua identidade cultural. A maior expressão desse culto é a grande romaria que os devotos realizam todos os anos no mês de outubro, que é conhecida como Círio de Nazaré e reúne uma média de dois milhões de pessoas, aproximadamente. O Círio de 2002 foi o recorte etnológico dessa pesquisa que disserta sobre as práticas cotidianas dos devotos no seu culto a Nossa Senhora de Nazaré, sobre como a religiosidade popular insere-se no dia-a-dia dos indivíduos, influenciando as representações sociais e recebendo a influência delas também, em um processo dialético.(AU)

A SENHORA DA BERLINDA Representações sociais do cotidiano na religiosidade popular paraense

O culto a Nossa Senhora de Nazaré é uma devoção popular muito antiga em Belém do Pará, quase tão antiga quanto a cidade, pois esse culto se consolidou na mesma época em que se constituiu o município de Belém e o Estado do Pará. A população de devotos cresceu junto com a população dos habitantes da cidade. Tal fato propiciou que essa devoção popular se enraizasse profundamente na cultura paraense, penetrando até a auto-imagem do paraense católico, moldando a sua identidade cultural. A maior expressão desse culto é a grande romaria que os devotos realizam todos os anos no mês de outubro, que é conhecida como Círio de Nazaré e reúne uma média de dois milhões de pessoas, aproximadamente. O Círio de 2002 foi o recorte etnológico dessa pesquisa que disserta sobre as práticas cotidianas dos devotos no seu culto a Nossa Senhora de Nazaré, sobre como a religiosidade popular insere-se no dia-a-dia dos indivíduos, influenciando as representações sociais e recebendo a influência delas também, em um processo dialético.(AU)

Latent varicella–zoster virus is located predominantly in neurons in human trigeminal ganglia

Varicella–zoster virus (VZV) is a human herpesvirus that causes varicella (chicken pox) as a primary infection and, after a variable period of latency in trigeminal and dorsal root ganglia, reactivates to cause herpes zoster (shingles). Both of these conditions may be followed by a variety of neurological complications, especially in immunocompromised individuals such as those with human immunodeficiency virus (HIV) infection. There have been a number of conflicting reports regarding the cellular location of latent VZV within human ganglia. To address this controversy we examined fixed wax-embedded trigeminal ganglia from 30 individuals obtained at autopsy, including 11 with HIV infection, 2 neonates, and 17 immunocompetent individuals, for the presence of latent VZV. Polymerase chain reaction (PCR), in situ hybridization, and PCR in situ amplification techniques with oligonucleotide probes and primer sequences to VZV genes 18, 21, 29, and 63 were used. VZV DNA in ganglia was detected in 15 individuals by using PCR alone, and in 12 individuals (6 normal non-HIV and 6 positive HIV individuals, but not neonatal ganglia) by using PCR in situ amplification. When in situ hybridization alone was used, 5 HIV-positive individuals and only 1 non-HIV individual showed VZV nucleic acid signals in ganglia. In all of the VZV-positive ganglia examined, VZV nucleic acid was detected in neuronal nuclei. Only occasional nonneuronal cells contained VZV DNA. We conclude from these studies that the neuron is the predominant site of latent VZV in human trigeminal ganglia.

Encapsulação de colecalciferol (vitamina D3) por spray chilling

A indústria de alimentos está constantemente desenvolvendo produtos que fornecem, além de nutrientes, benefícios adicionais à saúde, tais como os enriquecidos com vitaminas. A vitamina D3 (colecalciferol) é sintetizada na pele durante a exposição da luz solar, controla a homeostase de cálcio e fósforo, metabolismo ósseo, pressão arterial e reabsorção renal de cálcio. O processo de microencapsulação vem sendo bastante aplicado em alimentos e um dos objetivos principais é o controle da liberação do agente ativo no momento e local desejado. A tecnologia de spray chilling é interessante para a microencapsulação de vitaminas lipossolúveis. O objetivo deste trabalho foi microencapsular vitamina D3, utilizando o método de spray chilling para a produção das micropartículas lipídicas sólidas (MLS). Para produção das MLS utilizou-se gordura vegetal com ponto de fusão em torno de 48 °C como carreador. Três tratamentos foram estabelecidos: sem aditivos (T1), com adição de 1% de cera de abelha (T2) e com 1% de lecitina de soja (T3). As micropartículas foram caracterizadas quanto à morfologia por microscopia eletrônica de varredura, tamanho médio por difração a laser, espectroscopia no infravermelho por transformada de Fourier (FTIR) e foi analisada a estabilidade da vitamina D3 durante o armazenamento a 10 e 25 °C, por meio de quantificações periódicas em cromatografia líquida de alta eficiência (CLAE). As micropartículas obtidas foram esféricas, semelhantes morfologicamente e com distribuição monocaudal de partículas. O tamanho médio das partículas variou em função dos seus ingredientes, sendo que as micropartículas produzidas apenas com vitamina e gordura foram menores em relação às demais (83,0% < 100 µm). A espectroscopia na região do infravermelho (FTIR) demonstrou que não ocorreu interação entre os ingredientes. A estabilidade da vitamina D3 encapsulada foi satisfatória ao longo de 65 dias com valores superiores a 87% para os três tratamentos e a temperatura apresentou influência na estabilidade. As MLS produzidas com cera apresentaram melhores resultados de estabilidade de vitamina D3 com valores de 90,18 ± 2,23 % após 65 dias de estocagem. Esses resultados são promissores e demostram a viabilidade da técnica de spray chilling na produção de MLS carregadas de vitamina D3, possibilitando uma futura aplicação em alimentos.

Sistemática de Sericothripinae (Thysanoptera, Thripidae) e revisão taxonômica das espécies neotropicais

Esta tese é dividida em duas partes: traçar a filogenia de Sericothripinae Karny, 1921 e realizar uma revisão taxonômica das espécies neotropicais. Para a análise filogenética, estados de caracteres morfológicos foram examinados entre Sericothripinae e Thysanoptera-Terebrantia relacionados, e as relações genéricas e supragenéricas foram exploradas. O monofiletismo de Sericothripinae foi recuperado, mas o formato do metasterno, usado na classificação dos gêneros, provavelmente não reflete a filogenia. De acordo com estudos moleculares recentes, o grupo genérico Scirtothrips em conjunto com o gênero Echinothrips Moulton, 1911 foram recuperados como intimamente relacionados aos Sericothripinae, mas, neste trabalho, Psilothrips Hood, 1927 e Pseudothrips Hinds, 1902 foram recuperados como não relacionados com Sericothripinae. Muitos estados de caracteres morfológicos entre os Sericothripinae são considerados homoplásticos e, na ausência de análises moleculares adequadas, alterações formais de nomenclatura não foram realizadas. Na revisão taxonômica, 14 novas espécies de Sericothripinae da região Neotropical são descritas. Chaves ilustradas foram elaboradas para as fêmeas de sete espécies de Hydatothrips Karny, 1913 e 41 espécies de Neohydatothrips John, 1929, principalmente do Brasil, mas incluindo todas as espécies registradas do sul da fronteira entre o México e os EUA até o extremo sul da América do Sul. Espécies de plantas em que associações-hospedeiras foram registradas são indicadas sempre que possível, comentários são elencados para as poucas espécies de importância econômica e uma chave para imaturos de segundo instar de cinco espécies é proposta. Neohydatothrips burungae (Hood, 1935) stat. rev. e N. aztecus Johansen, 1983 stat. rev. são retiradas de sinonímia com N. signifer (Priesner, 1932), ao passo que Sericothrips denigratus De Santis, 1966 syn. n. é sinonimizada com N. burungae. Hydatothrips williamsi (Hood, 1928) comb. n. é realocado de Neohydatothrips e, com isso, houve um caso de homônimo no gênero. Para resolver esse problema, H. tareei nom. nov. é proposto para H. williamsi Mound e Tree, 2009, da Austrália.

Bioactive compounds and physical and chemical characteristics of mini tomatoes: influence of postharvest treatments

Tomatoes are among the most cultivated and used vegetables in the world. They are very succeptible to post harvest losses due to high perishability, therefore the use of postharvest treatments may contribute to conservation of this fruit, however the treatments might affect significantly physico-chemical, sensory and nutritional characteristics of tomatoes. Given the perishability of tomato and the economic importance of small tomato fruits, the purpose of the present study was to determine the effect of gamma radiation, carnauba coating and 1-MCP treatments on tomato fruit quality during storage. The study may be divided into two parts. In the first, mini tomatoes cv. Sweet Grape were harvested at breaker stage, divided into 4 grous and treated with gamma radiation (0.6 kGy), carnauba coating (1 L 1000 kg-1) and 1-MCP (500 nL L-1) and then stored at 25±2°C for 30 days with a control group of tomatoes. In the seconnd part, tomatoes harvested at light-red stage were submitted to the same treatments and storage period. Every 6 days tomatoes were evaluated for color modifications, fruit firmness, souble and total pectin (only for light-red tomatoes), mass loss, titratable acidity (TA), soluble solids (SS), SS/TA ratio, carotenoids profile, formation of lycopene isomers, total phenolic compounds, ascorbic acid and antioxidant capacity. For tomatoes harvested at breaker stage and submitted to the treatments the results showed mass loss was delaying mainly by carnauba wax, and to a lesser extend by 1-MCP. Fruit firmness were better retained for 1-MCP treated fruits and carnauba treatment showed a transient effect in preserving fruit firmness. SS/TA of tomatoes treated with gamma radiation and carnauba presented no differences from control values, and were lower with the application of 1-MCP. Color was negatively affected by 1-MCP and earlier changed (6th day) when gamma radiation was applied. In relation to bioactive compounds of tomatoes harvest at breaker stage, results indicated gamma radiation and 1-MCP decreased the final content of lycopene and produced more (Z)-isomers of lycopene. Gamma radiation also induced a decreased in ?-carotene and an increased in phenolic compounds by the end of storage period. 1-MCP treatment promoted a slow down increase in ascorbic acid content during storage. Antioxidant capacity of the hydrophilic fraction was not dramatically affected by treatments and the lipophilic fraction was lower, especially for 1-MCP fruits. In addition, contents of ?-carotene, lycopene, (Z)-isomers of lycopene, ascorbic acid and antioxidant capacity increased during the period of storage while contents of lutein and phenolic compounds tended to decrease. Regarding tomatoes harvest at light-red stage, the most effective treatments for delaying fruit firmness and mass loss was carnauba and 1-MCP, while gamma radiation was the treatment with higher mass loss and the less fruit firmness, which could be associated with the higher solubilization of pectins promoted by radiation treatment. Color (L* and Hue) was mainly affected by 1-MCP treatment which delayed color development, however, by the end of storage, the values were not different from the other treatments. SS/TA ratio was lower for fruits treated with 1-MCP and TA was not so dramatically affected by treatments. Furthermore, mini tomatoes harvested at light-red stage, demonstrated irradiation induced changes in the final content of lycopene, increasing it, and formed less (13Z)-lycopene, while 1-MCP and carnauba coating slow down the increase in lycopene and slown down the decrease of ascorbic acid and phenolic compounds. Antioxidant capacity of lipophilic fraction was not affected by treatments and the hydrophilic fraction was lower for irradiated fruits only on day 0 as well as phenolic compounds. In the other days, no differences among treatments were observed for hydrophilic antioxidant capacity. Considering the results, the best combination of SS and TA and fruit preservation for mini tomatoes harvest at breaker stage was promoted by carnauba coating, which seems to be the treatment that causes fewer changes in bioactive compounds of breaker tomatoes. However, when mini tomatoes were harvested at light-red stage, SS/TA ratio and color were better and, to preserve the quality of these fruits, besides carnauba coating, 1-MCP also could be indicated