Morfologia da micrópila e da superfície dos ovócitos de piracanjuba, Brycon orbignyanus (Osteichthyes, Characidae), sob microscopia eletrônica de varredura

This paper studied the morphology of oocytes of Brycon orbignyanus, (Osteichthyes, Characidae), through observation in Scanning Electron Microscopy (SEM). Fragments of the ovaries from adult females were collected. During the reproductive period, a hormonal induction in females was carried out to collect the oocytes after extrusion. The samples were fixed and processed for observation in SEM. Results showed that the oocytes of B. orbignyanus had a follicular epithelium formed by a single layer of cells with compressed shape, covering the whole radiatta zone that showed a smooth and regular surface with innumerable pores. The micropyle had a funnel-shaped, containing several furrows. The oocyte surface around the micropyle presented pores closer of each other than the other surface areas of radiatta zone.

Extração de matéria orgânica aquática por abaixamento de temperatura: Uma metodologia alternativa para manter a identidade da amostra

In this work was developed an alternative methodology to separation of aquatic organic matter (AOM) present in natural river waters. The process is based in temperature decreasing of the aqueous sample under controlled conditions that provoke the freezing of the sample and separation of the dark extract, not frozen and rich in organic matter. The results showed that speed of temperature decreasing exerts strongly influence in relative recovery of organic carbon, enrichment and time separation of the organic matter present in water samples. Elemental composition, infrared spectra and thermal analysis results showed that the alternative methodology is less aggressive possible in the attempt of maintaining the integrity of the sample.

Avaliação da formação do biofilme de Candida albicans sobre superfície de titânio revestida com hidroxiapatita por meio de microscopia eletrônica de varredura

In this study Candida albicans biofilm formation on the surface of commercially pure titanium (cp-Ti) coated with hydroxyapatite was observed by means of scanning electron microscope. The biofilm was formed after 45 days of incubation of the samples in liquid culture medium inoculated with fungus cells in a tube of polystyrene with screw cap and sterilized. After the biofilm removal with 10% EDTA solution was observed pitting on the surface of cp-Ti coated.

Efeito da temperatura ambiente e da restrição alimentar protéica ou energética sobre o ganho de peso e crescimento ósseo de frangos de corte

The effect of protein or energy restriction during the second week post-hatching on body weight gain and femur development of broiler chickens reared at different environmental temperatures (18°C, 25°C and 33°C) was studied. From 1 to 7 days of age and after a restriction period broilers were fed on a control diet with 2850 kcal ME/kg and 20% crude protein. From 8 to 14 days of age, two groups of broilers were fed on restricted energy and protein diets with 2565kcal ME/kg and 20% of crude protein or 2850kcal ME/kg and 15% of crude protein, respectively. At 14, 21, 28, 35 and 42 days, the bones were weighed and the length and width of bones measured. The protein restriction reduced the body weight gain and the diameter of bone at second week of life. After 21 days of age no differences between treatments for these traits were observed. The body weight gain and femur growth were not affected by energy restriction. The high temperature (33°C) reduced weight gain and femur diameter from 21 to 42 days of age, and femur length at 42 days of age. The protein restriction at second week or the high environmental temperature after 21 st day of life decreased body weight gain and femur growth of broiler chickens.

Geração de modelos digitais de superfície por meio de plataformas computacionais com estrutura vetorial e raster

Geographic Information Systems (GIS) integrate the technologies related to Geoprocessing, with the ability to manipulate georeferenced information through data storage, management and analysis. One of the GIS applications is the generation of Digital Elevation Models (DEM) as a result of rebuilding the elevation of a region using computation tools and artificial representation. This paper presents the DEM created from a point base in two computational frameworks with different structures (vector and raster), comparing the contour lines generated from these models with the original contour lines from analog cartographic base. It was observed that one of the generated models presented some discrepancies related to real space for both GIS structures. However, using constrained Delaunay's triangulation in raster GIS a digital elevation model was generated with contour lines quite close to the original ones, with satisfactory results. A 3-D terrain representation was also created offering a very useful tool for analysis.

Efeito da temperatura na germina̧ão de sementes de Sebastiania commersoniana (Baillon) Smith & Downs separadas pela colora̧ão do tegumento

Sebastiania commersoniana (Euphorbiaceae) is a tree species of riparian forests in Brazil. Seeds of this species released from mature fruits have heteromorphy in coat colour: dark (dark-brown to black), striated (dark with light-grey stria) and clear (light-grey to whitish). In this work two experiments were carried out in order to study the effect of temperature on seed germination in interaction with coat colour. Germination final percentage and speed index were evaluated. In the first experiment seeds of the three colours were submitted to constant (30°C) and alternating (20-35°C) temperatures. For all the seed colours, best results were obtained at alternating temperature. Physiological quality of striated seeds was greater than those clear seeds and dark seeds were intermediate. In the second experiment striated and clear seeds were submitted to three amplitudes of alternating temperature: 5°C (20-25°C and 25-30°C), 10°C (20-30°C and 25-35°C) and 15°C (20-35°C). Both germination percentage and speed were higher in striated seeds and for the two seed colours, best results were obtained at 20-30°C and 20-35°C. These temperatures are recommended for the germination test.

Modelagem numérica da superfície na geração da planialtimetra segundo dados de quatro equipamentos topogríficos

The aim of this present work was to compare planialtimetric charts obtained from different risings using two different theodolite types, a total station, and a precision level, used as control. Using a total station, an area was marked with clear variations of relief, following a grid, with a distance of 20 meters among stakes. After that, the stakes were read by the total station and two theodolites of different precisions. The geometric leveling was done by a precision level. The data were input in the DataGeosis software and the numerical modelling of the land was made with mesh of maximum rigidity, generating planialtimetric representation for each rising. It was verified, through comparison of the four representations that little variations occur in relation to the control. The closest representation of the control was the planialtimetry based on the data from the total station, in which the representations obtained from the theodolites were identical among themselves. It was concluded that in the process of obtaining detailed planialtimetry of small areas, submitted to the grid, it was not necessary to use composed geometric leveling, reducing work to the exclusive use of a total station or conventional theodolite.

Análise de superfície de tendência aplicada à chuva, medida por radar meteorológico, nas regiões de Assis e Piracicaba, SP

This paper presents the results from lineal trend surface analysis technique application. The purpose was to detect positive and negative anomalies in the rain measure distribution obtained by the meteorological radar Doppler, band S, located in Bauru, during the period of 21 of October/2004 to 29 of April/2005 in the areas of Assis and Piracicaba. Using three Z-R radar relations for rain quantification was chosen the specific equation Z = 32R1,65, as the best one. The results showed that the applied methodology was able to indicate the space distribution of the rain accumulated, identifying and locating the regions where there was rainy excess and rainy lack during each analyzed period. Such results indicate areas with larger pluvial impact and consequently more favorable for environmental damages.

Temperatura ambiente e concentrações séricas de cortisol em suínos em crescimento-terminação

Thirty-six castrated males and females Landrace x Large-White pigs (74 to 149 days of age) were randomly allotted to two environmental conditions: high temperature in a climatic chamber (HT; 22.2 to 32.8 °C) and comfort temperature in a conventional shed (CT; 17.6 to 26.6 °C), with night-and-day variations. Blood samples were weekly collected from animals of both HT and CT conditions for determination of serum cortisol levels. Cortisol levels of both sexes were not different, and there was no interaction with environmental temperature. Pigs of HT showed significantly higher average cortisol level (P<0.01) than the CT ones (7.06 and 4.82 mg/dL, respectively). Increasing in serum cortisol was continuous and linear (P<0.05) during the experimental period, suggesting the cortisol as a possible indicator of the heat stress in growing-finishing pigs.

Efeito da temperatura e tempo de austenitização nas transformações de fase da liga 13Cr2Ni0,1C

New classes of martensitic stainless steels, with low carbon levels, have been developed aiming to meet the needs of the petroleum industry segment. However, their use has been restricted due to the fact it is a recent development and many of its properties are still under investigation. This work determines the values of initial and final temperatures for the austenitic transformation and the initial and final temperatures of martensitic formation for alloy 13Cr2Ni0,1C, by means of dilatometric tests under continuous cooling. Based on these results the optimized conditions for quench and temper heat treatments were obtained. The microstructural characterization of the alloys under coarse fusion condition was carried out by optical microscopy and the presence of delta-ferrite in the martensitic matrix was observed.

Aplicação da metodologia de superfície de resposta na remediação de um solo arenoso contaminado artificialmente com cobre

Soil flushing is an alternative remediation technology for soils contaminated with heavy metals, which the main contaminant removing process consists in percolating an extraction solution. This work aimed to use the response surface methodology to point out combinations among the parameters of the extraction solution (Na2EDTA concentration, volume e pH) in order to reduce the concentration of copper in a sandy soil to risk levels lower than the intervention levels for exposure scenarios adopted by the Environmental Company of Sao Paulo State. Thus, a series of tests in leaching columns were carried out using a Fluvisol artificially contaminated (1257,3 mg kg-1). The tests were conducted in triplicate and setup a central composite rotatable design with 15 different parameters combinations of the extraction solution and one replicate in the center point. Using 5% significance level, the adjusted model (R2 = 0,98) indicated combinations of Na2EDTA concentration, pH and volume of the extraction solution which allow reduction of copper concentration below levels reported by environmental agency of Sao Paulo State for industrial, residential, agricultural or maximum exposure scenarios.

Química mineral e estimativas de pressão e temperatura em rochas metassedimentares do grupo araxá na região de morrinhos, sul do estado de goiás

In the Morrinhos region, Goiás state, Brazil, the rocks of the Araxá Group have mineral assemblages related to the metamorphic peak which occurred in this region and is typical of the amphibolite facies (kyanite zone). These rocks are associated with the initial stage of the main ductile deformation that occurred in this area. Thermobarometric calculations, including the associations, garnet + plagioclase + biotite + muscovite; garnet + plagioclase +biotite + muscovite + kyanite; garnet + plagioclase + biotite + muscovite + amphibole, with quartz in excess, indicate that the metamorphic peak occurred at temperatures of approximately 610°C and pressures in the order of 10 kbar. The P-T path is probably clockwise and is in agreement with the tectonic conditions observed in collisional settings.

Influência de protocolos de jateamento na rugosidade da superfície de uma cerâmica de zircônia tetragonal parcialmente estabilizada por ítria

Objective: To evaluate the influence of different air abrasion protocols on the surface roughness of an yttria-stabilized polycrystalline tetragonal zirconia) (Y-TZP) ceramic, as well as the surface topography of the ceramic after the treatment. Method: Fifty-four specimens (7.5×4×7.5mm) obtained from two ceramic blocks (LAVA, 3M ESPE) were flattened with fine-grit sandpaper and subjected to sintering in the ceramic system's specific firing oven. Next, the specimens were embedded in acrylic resin and the surfaces to be treated were polished in a polishing machine using sandpapers of decreasing abrasion (600- to 1,200-grit) followed by felt discs with 10μm and 3μm polishing pastes and colloidal silica. The specimens were then randomly assigned to 9 groups, according to factors particle and pressure(n=6): Gr1- control; Gr2- Al 2O 3(50μm)/2.5 bar; Gr3- Al 2O 3(110μm)/2.5 bar; Gr4- SiO 2(30μm)/2.5 bar; Gr5- SiO 2(30μm)/2.5 bar; Gr6- Al 2O 3(50μm)/3.5 bar; Gr7- Al2O3(110μm)/3.5 bar; Gr8- SiO 2(30μm)/3.5 bar; Gr9- SiO 2(30μm)/3.5 bar. After treatments, surface roughness was analyzed by a digital optical profilometer and the morphology was examined by scanning electron microscopy (SEM). Data (μm) were subjected to statistical analysis by Dunnett's test (5%), two-way ANOVA and Tukey's test (5%). Results: The type of particle (p=0.0001) and the pressure (p=0.0001) used in the air abrasion protocols influenced the surface roughness values among the experimental groups (ANOVA). The mean surface roughness values (μm) obtained for the experimental groups (Gr2 to Gr9) were, respectively: 0.37 D; 0.56 BC; 0.46 BC; 0.48 CD; 0.59 BC; 0.82 A; 0.53B CD; 0.67 AB. The SEM analysis revealed that Al 2O 3, regardless of the particle size and pressure used, caused damage to the surface of the specimens, as it produced superficial damages on the ceramic, in the form of grooves and cracks. Conclusion: Al2O3 (110 μm/3.5 bar) air abrasion promoted the highest surface roughness on the ceramics, but it does not mean that this protocol promotes better ceramic-cement union compared to the other air abrasion protocols.

Parâmetros físicos pré-eruptivos do magmatismo ácido da província magmática do Paraná: Resultados preliminares

A set of 12 samples of acid rock types Palmas (ATP) and Chapecó (ATC) was used to determine the chemical composition of plagioclase and pyroxene by electron microprobe, with the purpose to get information about the pressure and temperature of crystallization of these rocks. The results show that the pyroxene of ATP rocks (3,2 ± 1,2 kbar, max = 5,1 kbar and 1028 ± 38°C) were formed under pressure conditions higher than those ATC (1,8 ± 0,9 kbar, max = 3,4 kbar and 995 ± 26oC). However, the pressures obtained from plagioclase showed higher pressures for ATC (3.2 ± 1 kbar, max = 6,4 kbar and 1033 ± 12°C) than ATP (1,9 ± 1 kbar, max = 4,8 kbar and 1043 ± 5°C), suggesting that the crystallization of rocktype ATP began with the formation of pyroxene and plagioclase almost simultaneously at a depth of around 17 km while the ATC, began with the crystallization of plagioclase at a depth of about 21 km (assuming a gradient of 3,3 kbar/km). The geothermometry of plagioclase allow us to calculate the concentration of water from about 1 ± 0,3% H2O for both acid rock types. Additional calculations allow us to get the depth of water exsolution of magmatic liquid at 30m below the surface. Although the data are still preliminary and insufficient to model the extrusion of these rocks, they point out to an effusion mechanism of a partially fluidized magma by volatile, which would spread to large areas with small friction with the surface that would increased with the increase of viscosity caused by the loss of volatile and decreasing of temperature, developing coherent structures as lava flows.

Maçã 'Royal Gala' revestida com quitosana estocada à temperatura ambiente

The aim of this work was to evaluate apples covered with chitosan during storage at ambient temperature. 'Royal Gala' apples were covered with chitosan (MRQ), immersed in acetic acid solution (MB) or did not receive any treatment (MC) and subsequently analyzed physicochemical, sensorial andinstrumentally, as well as for rotten occurrence, at zero, 7, 14, 21, 28, 35, 42 and 46 days of storage. The ratio, pH and reducing sugar contents increased while total titratable acidity, total sugars, total solids and firmness were reduced during storage. Was observed a drastically reduction of ascorbic acid and slight increase on soluble solids, except in the apples that were not pretreated (MC). Luminosity and chromaticity ratio values improved with time, with higher intensity in MRQ. Rotten occurrence was not expressive. The acceptance of appearance, color, global impression, aroma and flavor decreased with time, and appearance and texture were considered the most important sensory attributes for apples. The use of chitosan delayed ripening, reduced losses in firmness and presented a more intense bright, extending shelf life period up to seven days.