Yellow Perch Population Assessment in Southwestern Lake Michigan

Division of Fisheries, Illinois Department of Natural Resources Grant/Contract No: Federal Aid Project F-123 R-16

Yellow Perch Population Assessment in Southwestern Lake Michigan, Including Evaluation of Sampling Techniques and Identification of the Factors that Determine Yellow Perch Year-Class Strength April 1, 2007 – March 31, 2008

Division of Fisheries, Illinois Department of Natural Resources Grant/Contract No: F-123 R-14

Yellow perch population assessment in southwestern Lake Michigan, including evaluation of sampling techniques and identification of the factors that determine yellow perch year-class strength April 1, 2006 – March 31, 2007

Division of Fisheries, Illinois Department of Natural Resources Grant/Contract No: F-123 R-13

Yellow Perch Population Assessment in Southwestern Lake Michigan, Including Evaluation of Sampling Techniques and Identification of the Factors that Determine Yellow Perch Year-Class Strength April 1, 2008 – June 30, 2009

Division of Fisheries, Illinois Department of Natural Resources Grant/Contract No: Federal Aid Project F-123 R-15

COMPOSIÇÃO FLORÍSTICO-ESTRUTURAL AO LONGO DE UM GRADIENTE DE BORDA EM FRAGMENTO DE FLORESTA OMBRÓFILA MISTA ALTO-MONTANA EM SANTA CATARINA

O objetivo desse estudo foi caracterizar a composição florística e a estrutura do componente arbóreo em fragmento de Floresta Ombrófila Mista Alto-Montana e avaliar a influência do efeito de borda sobre a organização, estrutura, riqueza e diversidade de espécies. Foram alocadas 50 parcelas permanentes de 10 x 20 m, divididas em cinco transeções distanciadas, no mínimo, 100 m entre si, em um fragmento florestal, no município de Bom Jardim da Serra - SC. As árvores com circunferência ≥ 15,7 cm na altura do peito (CAP) foram mensuradas (CAP e altura total), identificadas e classificadas quanto às guildas de regeneração (pioneiras, climácicas exigentes em luz e climácicas tolerantes à sombra). Os dados foram analisados por meio dos índices de valor de importância (IVI), NMDS (Nonmetric Multidimensional Scaling), modelo aditivo generalizado e regressões lineares simples. Foram observados 1.457 indivíduos arbóreos, distribuídos em 29 famílias, 43 gêneros e 55 espécies. A espécie com maior valor de importância foi Dicksonia sellowiana Hook. Não foi observada influência do efeito de borda sobre a organização, a estrutura (diâmetro médio, altura média e densidade) da comunidade e participação relativa das guildas de regeneração. No entanto, ficaram evidenciados maiores valores de diversidade, riqueza e equabilidade nas áreas de borda. Desta forma, concluí-se que parte das variações dos valores relativos à diversidade de espécies arbóreas na Floresta Ombrófila Mista Ato-Montana foi determinada pela distância da borda.

GENETIC DIVERSITY AMONG SORGHUM LANDRACES OF SOUTHWESTERN HIGHLANDS OF UGANDA

Sorghum ( Sorghum bicolor L. Moench) is an economic and staple crop in sub-Saharan Africa. The genetic diversity in its germplasm is an invaluable aid for its crop improvement. The objective of this study was to assess the existing genetic diversity among sorghum landraces in the southwestern highlands of Uganda. A total of 47 sorghum landraces, collected from southwestern highlands of Uganda, were characterised using 12 qualitative and 13 quantitative traits. The study was conducted at Kachwekano Research Farm in Kabale District, at an altitude of 2,223 m above sea level, during growing season of December 2014 to August 2015. Panicle shape and compactness were the most varied qualitative traits. Grain yield (1.23 to 11.31 t ha-1) and plant height (144.7 to 351.6 cm) were among quantitative traits that showed high variability. Days to 50% flowering (115 to 130 days) showed the least variability. Results of UPGMA cluster analysis generated a dendrogram with three clusters. Panicle weight, leaf width, stem girth, exertion length, peduncle length, panicle shape and compactness, glume colour and threshability were major traits responsible for the observed clustering (P<0.001). Principal Component Analysis revealed the largest variation contributors.

PCR-Internal Transcribed Spacer (ITS) genes sequencing and phylogenetic analysis of clinical and environmental Aspergillus species associated with HIV-TB co infected patients in a hospital in Abeokuta, southwestern Nigeria.

Background: Aspergillosis has been identified as one of the hospital acquired infections but the contribution of water and inhouse air as possible sources of Aspergillus infection in immunocompromised individuals like HIV-TB patients have not been studied in any hospital setting in Nigeria. Objective: To identify and investigate genetic relationship between clinical and environmental Aspergillus species associated with HIV-TB co infected patients. Methods: DNA extraction, purification, amplification and sequencing of Internal Transcribed Spacer (ITS) genes were performed using standard protocols. Similarity search using BLAST on NCBI was used for species identification and MEGA 5.0 was used for phylogenetic analysis. Results: Analyses of sequenced ITS genes of selected fourteen (14) Aspergillus isolates identified in the GenBank database revealed Aspergillus niger (28.57%), Aspergillus tubingensis (7.14%), Aspergillus flavus (7.14%) and Aspergillus fumigatus (57.14%). Aspergillus in sputum of HIV patients were Aspergillus niger, A. fumigatus, A. tubingensis and A. flavus. Also, A. niger and A. fumigatus were identified from water and open-air. Phylogenetic analysis of sequences yielded genetic relatedness between clinical and environmental isolates. Conclusion: Water and air in health care settings in Nigeria are important sources of Aspergillus sp. for HIV-TB patients.

Effects of gamma and electron beam irradiation in the antioxidante potential of methanolic extracts and infusions of Arenaria Montana L

Irradiation is a methodology qualified for dry ingredients preservation or decontamination and can be performed using various radiation sources and energy levels in accordance with the objectives to be achieved [1]. Electron beam irradiation is used mainly for food products with low density, while gamma irradiation is mainly used for large volumes [2]. Arenaria Montana L. has a high antioxidant potential and richness in bioactive phytochemicals. It is used in Portuguese traditional medicine, acting therapeutically as an anti-inflammatory and diuretic plant [3]. The aim of this work was to evaluate the effects of gamma and electron beam irradiation at different doses (I and 10 kGy) in the antioxidant activity of A. montana. Free radicals scavenging activity, reducing power and lipid peroxidation inhibition properties of its methanolic extracts and infusions were evaluated. Through a global analysis, it was concluded that the antioxidant activity proved to be higher in methanolic extracts in comparison with the infusions, where it decreased with increasing irradiation dose regardless of the technology used (gamma or electron beam). For methanolic extracts, electron beam resulted in increased antioxidant activity while gamma irradiation caused a decrease in these extracts. Thus, the antioxidant potential is variable depending not only on the type of radiation and the dose applied, but also on the solvent used in the preparation of the extracts (methanol or water).

Description of a new species of skate of the genus Malacoraja Stehmann, 1970: the first species from the southwestern Atlantic Ocean, with notes on generic monophyly and composition (Chondrichthyes: Rajidae)

O primeiro registro para o Atlântico Sul ocidental de uma espécie do gênero Malacoraja Stehmann, 1970 é feita com base na descrição de Malacoraja obscura, espécie nova, proveniente do talude continental do Sudeste brasileiro dos estados do Espírito Santo e Rio de Janeiro em profundidades de 808-1105 m. A espécie nova é conhecida através de cinco exemplares e é distinta de seus congêneres pela sua coloração dorsal composta por numerosas manchas esbranquiçadas e pequenas na região do disco e nadadeiras pélvicas, por apresentar uma fileira irregular de espinhos ao longo da superfície dorsal mediana da cauda a qual persiste em espécimes maiores (desde a base da cauda até dois-terços do seu comprimento numa fêmea de 680 mm de comprimento total, CT) e uma região pequena desprovida de dentículos na base ventral da cauda (estendendo somente até a margem distal da nadadeira pélvica). Outros caracteres diagnósticos em combinação incluem a ausência de espinhos escapulares em indivíduos maiores, número elevado de fileiras dentárias (64/62 fileiras num macho subadulto de 505 mm de CT e 76/74 numa fêmea de 680 mm de CT) e de vértebras (27-28 Vtr, 68-75 Vprd), coloração ventral do disco uniformemente castanha escura, duas fenestras pós-ventrais na cintura escapular, fenestra pós-ventral posterior grande, forame magno circular e dois forames para a carótida interna na placa basal ventral do neurocrânio. Machos adultos não são conhecidos, porém uma descrição anatômica de M. obscura, sp. nov., é fornecida. Comparações são realizadas com todo o material conhecido de M. kreffti, com a literatura sobre M. senta e com material abundante de M. spinacidermis da África do Sul; M. obscura, sp. nov., assemelha-se mais a M. spinacidermis do Atlântico Sul oriental em esqueleto dérmico, coloração e tamanho. Malacoraja é monofilético devido à sua espinulação e apêndices rostrais conspícuos e é aparentemente composta por dois grupos de espécies, um para M. obscura e M. spinacidermis e outro para M. kreffti e M. senta, porém a elucidação das relações filogenéticas entre as espécies necessita de mais informações anatômicas, principalmente das duas últimas espécies.

DIET of THE BLUE MARLIN (MAKAIRA NIGRICANS, LACEPEDE 1802) (PERCIFORMES: ISTIOPHORIDAE) of THE SOUTHWESTERN EQUATORIAL ATLANTIC OCEAN.

Diet composition of the blue marlin, Makaira nigricans, from the southwestern equatorial Atlantic Ocean was analyzed between October 2004 and November 2005. In the 226 stomachs of fish ranging between 100 and 311 cm lower jaw -fork length (LJFL), 44 items were identified, including 31 fishes and 13 cephalopods. Seventy stomachs were empty (23.6 %). M. nigricans fed preferentially on heavy and muscular scombrid fishes especially upon the skipjack tuna, Katsuwonus pelamis (Linnaeus, 1758), probably to sustain their high metabolism, and on a variety of other items composed mainly by epipelagic species of fish and cephalopods.

Research, STEM Education, & Native American Programs in Montana

Oscillating electroosmotic flows in microchannels can be used as a separating mechanism for biological species. This MEMS related work specifically studies the separation of different sized DNA on the microscale as a pre-cursor to lab on a chip systems. In addition to this research, Dr. Thomas will present programs and activities that enable Native American students to obtain their STEM degrees. This involves working with middle school through graduate students at the 4 year institutions, Tribal colleges, and K-12 schools in Montana.

Montana Style: The Koch Bros. & Talking Cows

A Montana Public Radio Commentary by Evan Barrett. Published newspaper columns written by Evan Barrett on this topic, which vary somewhat in content from this commentary, appeared in the following publications: Montana Standard, June 2, 2015 Ravalli Republic, June 4, 2015 Missoulian, June 5, 2015 Montana Public Radio, June 8, 2015

A Hydrogeologic Evaluation of the Waterloo Area in the Upper Jefferson River Valley, Montana

The Upper Jefferson River is one of the most dewatered rivers in Montana. The river exists in an intermontane basin filled with sediment transported from the Highland Mountains to the west, the Tobacco Root Mountains to the east, and the Jefferson River from the south. The Upper Jefferson River Valley is highly dependent on the Jefferson River as the main industry in the valley is agriculture. A majority of the valley is irrigated and used to grow crops, and a good portion is also used for cattle grazing. The residents of the Upper Jefferson River Valley use the aquifer as the main source of potable water. The Jefferson River is also widely used for recreation. This study took place in the Waterloo area of the Upper Jefferson River Valley, approximately 20 miles south of Whitehall, Montana. The Waterloo area provides significant groundwater base flow to the Jefferson River, which is particularly important during the late irrigation season when the river is severely dewatered, and elevated surface-water temperatures occur, creating irrigation water shortages and poor trout habitat. This area contains two springfed streams, Willow Springs and Parson’s Slough, which discharge to the Jefferson River providing cool water in the late season as well as providing the most important trout spawning habitat in the valley. The area is bordered on both the east and west by irrigation ditches, and about 60% of the study area is irrigated. Tile drains were installed in the study area in close proximity to Parsons Slough causing some concern by neighboring residents. This study evaluated relationships between surface water, groundwater, and irrigation practices so that water managers and others can make informed management decisions about the Upper Jefferson River. Data was collected via a network of groundwater wells and surface-water sites. Additionally, water-quality samples were taken and an aquifer test was conducted to determine aquifer properties. The field data were analyzed and a groundwater budget was created in order to evaluate the aquifer. Results of the groundwater budget show that seepage from the irrigation canals and irrigation recharge have the biggest influence on recharge of the aquifer. There is significant groundwater outflow from the aquifer in the spring-fed streams as well as discharge to the Jefferson River. In comparing previous study results to this study’s results, there is no evidence of the water table decreasing due to irrigation practice changes or tile drain installation. However, given the amount of recharge irrigation practices contribute to the aquifer, if significant changes were made, they may affect groundwater elevations. Also lining the irrigation ditches would have a significant impact on the aquifer, as the amount of seepage would be greatly reduced.

Shallow Groundwater Quality and Geochemistry in the Shields River Basin, South-Central Montana

Water samples were collected from 33 domestic wells, 2 springs, and 3 streams in the Shields River Basin (Basin) in southwest Montana. Samples were collected in 2013 to describe the chemical quality of groundwater in the Basin. Sampling was done to assess potential impacts to water quality from recent exploratory oil and gas drilling and to establish baseline water quality conditions. Wells were selected in areas near and away from oil and gas drilling and in areas susceptible to contamination. Water samples from surface water sites were collected in October to characterize base flow conditions. Physical characteristics of the land surface, soils, and shallow aquifers were used to assess groundwater susceptibility to contamination from the land surface. This analysis was completed using GIS. Samples were analyzed for major ions, trace metals, water isotopes of oxygen and hydrogen. A subset (24) of samples were analyzed for tritium and organic constituents (GRO, DRO, BTEX, methane, ethylene, and ethane). One sample exceeded the human health drinking water standard for selenium. Dissolved methane and ethylene gas were detected in six samples at concentrations less than 0.184 milligrams per liter. Three locations were resampled in 2014, and no methane or ethylene was detected. Shallow groundwater and streams are generally calcium- or sodium-bicarbonate type water with total dissolved solids concentration less than 300 milligrams per liter. Some wells produce either sodium-chloride or sodium-sulfate type water suggesting slower flow paths and more rock-water interaction. Tritium concentrations suggest that older water (TU< 0.8), recharged prior to the mid-1950’s, is generally sodium type, whereas younger water (TU > 4) is generally a calcium type. Water-quality data from this study were compared to available historic data in the Basin. Additionally, the USGS Produced Waters Geochemical database was queried for chemical data of produced waters from reservoir rocks throughout Montana and the surrounding states. Comparisons to historic and produced water chemical data suggest no impact to shallow groundwater quality from exploratory oil and gas drilling.

Reproductive aspects of the oceanic whitetip shark, Carcharhinus longimanus (Elasmobranchii: Carcharhinidae), in the equatorial and southwestern Atlantic Ocean

The present study sought to study the reproductive biology of the oceanic whitetip shark, Carcharhinus longimanus, in the equatorial and southwestern Atlantic Ocean. A total of 234 specimens were collected as bycatch during pelagic longline fisheries targeting tunas and swordfish, between December 2003 and December 2010. The fishing area was located between latitudes 10N and 35S and longitudes 3E and 40W. Of the 234 individuals sampled, 118 were females (with sizes ranging from 81 to 227 cm TL, total length) and 116 males (ranging from 80 to 242 cm TL). The reproductive stages of the females were classed as immature, mature, preovulatory and pregnant, while males were divided into immature, maturing and mature. The size at maturity for females was estimated at 170.0 cm TL, while that for males was between 170.0 and 190.0 cm TL. Ovarian fecundity ranged from 1 to 10 follicles and uterine fecundity from 1 to 10 embryos. The reproductive cycle of this species is most likely biennial, with parturition occurring once every two years.