958 resultados para Sinoglottidia gen. nov


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A polyphasic taxonomic analysis was carried out on 11 uncommon Gram-stain-negative, non-motile, catalase- and oxidase-positive, but indole-negative, bacterial strains isolated from tortoises. Phenotypically and genetically they represented a homogeneous group of organisms most closely related to, but distinct from, Uruburuella suis. In a reconstructed 16S rRNA gene tree they clustered on a monophyletic branch next to U. suis with gene similarities between strains of 99.5-100%, and of up to 98.2% with U. suis . DNA-DNA hybridization indicated the organisms represented a novel species with only 40% DNA-DNA similarity with U. suis . Partial sequencing of rpoB resulted in two subclusters confirming the 16S rRNA gene phylogeny; both genes allowed clear separation and identification of the novel species. Furthermore, they could be unambiguously identified by matrix-assisted laser desorption ionization time-of-flight MS, where, again, they formed a highly homogeneous cluster separate from U. suis and other members of the family Neisseriaceae . The major fatty acids were C(16 : 0) and summed feature C(16 : 1)ω7c/iso-C(15 : 0) 2-OH. The DNA G+C content was 54.4 mol%. Based on phenotypic and genetic data we propose classifying these organisms as representatives of a novel species named Uruburuella testudinis sp. nov. The type strain is 07_OD624(T) ( = DSM 26510(T) = CCUG 63373(T)).

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Vorbesitzer: Bartholomaeusstift Frankfurt am Main

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Welsch (Projektbearbeiter): Aufruf an die Bevölkerung Böhmens, sich nicht vom "Kampf des Parteihasses" hinreißen zu lassen. Warnung vor dem Bürgerkrieg und Hinweis auf die gewährten Zugeständnisse und Freiheiten

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Welsch (Projektbearbeiter): Kampfgedicht gegen den am 22. März 1848 nach England geflohenen Prinzen von Preußen, die Symbolfigur der bewaffneten Reaktion

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Welsch (Projektbearbeiter): Feierliches Gedicht zu Ehren der Frankfurter Nationalversammlung. Bekenntnis zu Freiheit und Einheit sowie zur allgemeinen Wehrpflicht

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Welsch (Projektbearbeiter): Frisch-fröhliches Gedicht anläßlich der Niederschlagung der badischen revolutionären Aufstände vom April und September 1848 mit Hilfe von Bundestruppen des VIII. Armeekorps (württembergisches, hessisches und nassauisches Militär)

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Welsch (Projektbearbeiter): Weder die Preußische Nationalversammlung noch das Ministerium Camphausen, weder das Militär, noch der Berliner Magistrat treten für die Rechte, das Wohl und die Freiheit des Volkes ein; dies kann nur das Volk selbst tun. "Alles ist faul, oberfaul - nur das Volk in seinem gesunden Kerne nicht. Verlaßt euch auf euch selbst! Stehet oder fallet Alle für einen Mann!"

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Five isolates of non-pigmented, rapidly growing mycobacteria were isolated from three patients and,in an earlier study, from zebrafish. Phenotypic and molecular tests confirmed that these isolates belong to the Mycobacterium chelonae-Mycobacterium abscessus group, but they could not be confidently assigned to any known species of this group. Phenotypic analysis and biochemical tests were not helpful for distinguishing these isolates from other members of the M. chelonae–M.abscessus group. The isolates presented higher drug resistance in comparison with other members of the group, showing susceptibility only to clarithromycin. The five isolates showed a unique PCR restriction analysis pattern of the hsp65 gene, 100 % similarity in 16S rRNA gene and hsp65 sequences and 1-2 nt differences in rpoB and internal transcribed spacer (ITS) sequences.Phylogenetic analysis of a concatenated dataset including 16S rRNA gene, hsp65, and rpoB sequences from type strains of more closely related species placed the five isolates together, as a distinct lineage from previously described species, suggesting a sister relationship to a group consisting of M. chelonae, Mycobacterium salmoniphilum, Mycobacterium franklinii and Mycobacterium immunogenum. DNA–DNA hybridization values .70 % confirmed that the five isolates belong to the same species, while values ,70 % between one of the isolates and the type strains of M. chelonae and M. abscessus confirmed that the isolates belong to a distinct species. The polyphasic characterization of these isolates, supported by DNA–DNA hybridization results,demonstrated that they share characteristics with M. chelonae–M. abscessus members, butconstitute a different species, for which the name Mycobacterium saopaulense sp. nov. is proposed. The type strain is EPM10906T (5CCUG 66554T5LMG 28586T5INCQS 0733T).

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Robert Eisler

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Julius Böhmer