Atividade antioxidante e anti-inflamatória da hesperidina glicosilada em camundongos submetidos a dieta indutora do estado pró-inflamatório

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Políticas Públicas de Resíduos Sólidos e a Coleta Seletiva em Regente Feijó - SP

This research was mainly focused on public policy analysis of solid waste, and the National Policy and State Solid Waste, as well as determining the performance of the Blue Green Municipality strategic project in relation to the management of municipal solid waste in Regente Feijó, São Paulo. The objective of this research was to analysis of the consequences of the deployment project of selective collection and integration of recyclable material collectors in an association of collectors, called the Association of Recyclable Material Collectors Rock, held in the city from the year 2008, with effective implementation in 2010. We analyzed the actions of the municipal government since the implementation of selective collection, to the development of the Association. The methodology used was literature concerning the issue; routine field surveys to monitor the activities in the landfill and association of collectors, collecting data from questionnaires appropriate to the research topic. We sought to examine old and new forms of urban solid waste management, the evolution of the historical process of this issue in the city and its social and environmental impacts, and the collectors the main social actors in this process. The complexity that addresses this issue was dealt with in this dynamic study, analyzing all parts involved

Síntese e estudo da diapocinina como um inibidor da NADPH Oxidase em neutrófilos e células mononucleares de sangue periférico

Apocynin, a methoxy-catechol originally extracted from the root of Picrorhiza kurroa, has been used as an inhibitor of the NADPH oxidase complex in phagocytic and nonphagocytic cells. Its mechanism of inhibition is linked to their prior activation through the action of peroxidases leading to oxidation of the dimeric product, diapocynin. In this study, dipocinina was synthesized and investigated its effect as an inhibitor of activation NADPH oxidase in neutrophils (PMN) and peripheral blood mononuclear cells (PBMC). The synthesis of diapocinina was performed by oxidation of apocinina by potassium persulphate in the midst of water for 5 minutes at room temperature. The precipitate was filtered and washed with water and methanol. Diapocinina was characterized by mass spectrometry. PMN and PBMC were obtained from peripheral blood of healthy donors and purified for gelatin sedimentation, or centrifugation with Histopaque ®, the red cells were lysed with ice water or ammonium chloride. Diapocinina or apocinina were incubated with opsonized zymosan, activation of PMNs and release of superoxide anion, these monitored by chemiluminescent assay dependent lucigenina. We found that diapocinina inhibitor was no better than the apocinina in PMN. However, diapocinina was more efficient than apocinina as an inhibitor of NADPH oxidase in PBMC. In conclusion, whereas PBMC are relatively poor compared with peroxidases PMN, our results are consistent with the need for oxidation apocinina for its effect as an inhibitor of NADPH oxidase

Mapeamento do uso da terra como subsídio a um plano de recuperação das áreas de preservação permanente no entorno da represa de Salto Grande - Americana - SP

Currently, the dam of Salto Grande is very degraded. Water quality is seriously amended, and lack of riparian vegetation, as well as the advance of feed crops and land occupation in areas that should be permanently preserved, only complicate this situation. The permanent preservation area is essential for the water sources protection, soil erosion control and consequent watercourse sedimentation. Through aerial photography, using GIS techniques, it was able to identify the outwards bounds of the dam, to demarcate the permanent preservation areas and generate maps for land use. With this data, in addition to studies and ideas of reforestation in different environments, the development of a plan for restoration of degraded areas surrounding the dam is easier to be done. This study confirmed that about 72% of land use in permanent preservation areas is not in accordance with specific laws, making necessary its recovering.

Uso de algoritmos genéticos na otimização do sistema de coleta do palhiço de cana-de-açúcar

The Brazilian government has convinced the world that ethanol deriving from sugar cane is a promissory means of sustainable fuel for vehicles. There is a great growth of ex vehicles , i.e, run both by ethanol and gasoline, due to competent automotive industries and e cient alcohol production technology. In 2009 and 2010 the ethanol production was 25.7 billion liters and 53.8% of sugar cane production was destined to alcohol production. Nevertheless, the sugar production also derived from sugar cane should increase in 2011. Brazil produced 33 million tons of sugar in the last harvest. With sugar cane on the rise production is arising new environmental problems. The harvest using mechanized cut besides improving the logistic transportation system leaves the generating residue in the eld. This residue is a mixture of straw, leavings and scrap of sugar cane named sugar cane crop residue and corresponds to 30% of biomass and can be burned and produce electricity by cogeneration. But the transport the sugar cane crop from the eld is expensive due costs involved in the transport system. This work aims to propose a formulation for the bales collecting problem from sugar cane eld to mill that minimize the costs involved in the transport system. The computational tests use the C++ language and an algorithm based on genetic algorithms techniques

Diagnóstico ambiental das nascentes do Córrego dos Macacos – município de Álvares Machado/SP

Environmental diagnosis fits in analysis social-spatial organization insofar it contributes to understanding past and present aspects of how a territory works as well as enables the creation of statements for future ordainment. In this context, the present study aims at contributing with data for creating recovery and environmental preservation projects for the basin of Macacos‟ stream‟s headwaters, located in the north of Álvares Machado county in São Paulo state. To achieve this purpose, the main characteristics and existing uses in the basin have been analyzed and its main environmental problems were identified in the data and information collected in journals, field work and interviews with dwellers from the interested area and local government authorities. On this way, a diagnosis of the environmental state of the studied area was performed considering its specific issues regarding the natural and physical process as well as human influences. From the analysis carried out it was possible to identify environmental impacts on different levels resulting from the conflictuous relation between uses and occupations and the natural dynamics of the studied basin.

Técnicas de Bioengenharia Aplicadas ás Áreas de Preservação Permanente Urbanas – Presidente Prudente, São Paulo

There is a demand to incorporate the nature in the city in order to meet the wishes of the entire population. The valuation of water, a key component of the quality of the landscape, becomes a key element in this context both for its ecological potential because of their potential for recreational activities. The areas that suffer most impacts are the Permanent Preservation Areas (PPAs) and this paper will be a weighting on the urban interface - PPA. With the study area the basin of the stream of Limoeiro, in the context of the Friendship Fountain Balneary, especially the source contained between residential neighborhoods Maré Mansa and Parque Imperial, the city of Presidente Prudente and Álvares Machado, Sao Paulo, this paper aims to search and use of knowledge about technologies and techniques of environmental restoration, with emphasis on bioengineering, through an environmental characterization of the basin, with a view the incorporation of nature in the lives of the urban population. The methodology includes: literature review priority issues related to the PPAs, linear parks, relevant environmental legislation, environmental characterization of the watershed of the stream Limoeiro; study of technologies and techniques for environmental remediation of soil bioengineering and implementation of parks linear, development of proposals for the environmental recovery of PPA in the basin of the stream Limoeiro, focusing on emerging... (Complete abstract click electronic access below)

IL-10 e TNF-α no sangue materno e nas placentas de gestações complicadas por diabete ou hiperglicemia gestacional leve - correlação com controle glicêmico e resultados perinatais

Objective - At evaluating the IL-10 production in maternal blood and placenta and correlate them with perinatal outcomes in pregnancies complicated by hyperglycemia, or with risk to developing it, differentiated by the glycemic mean (GM < or ≥ 100mg/dL). Method- 186 pregnant women were distributed into groups GM < 100 mg/dL and GM ≥ 100 mg/dL. We evaluated the GM, HbA1c levels, maternal and placental IL-10 and TNF-α and the correlation between placental cytokines and perinatal outcomes. Results - In maternal blood, the lower concentrations of IL-10 (1.01 ± 0.87 vs. 3.08 ± 5.57 pg / mL, p = 0.0019) were observed in GM ≥ 100 mg / dL group. Placental IL-10 was directly correlated with hemoglobin levels (r = 0.63, p = 0.02) and insulin (r = 0.78, p = 0.01) from umbilical cord and with Apgar scores 1 (r = 0.53, p = 0.0095) and Apgar 5 (r = 0.69, p = 0.0003). Conclusion - GM ≥ 100mg/dL was associated with decreased of maternal IL-10. Placental IL-10 was similar in both groups and correlated directly with hemoglobin and insulin and with Apgar scores of 1st. and 5th. minutes

A questão cultural no município de Álvares Machado: a importância de políticas públicas no processo de preservação urbano-ambiental

This work analyzes the importance of public policies in the process of urban-environmental preservation of the Alvares Machado city at state of Sao Paulo in Brazil, where priority was given to questions related to cultural heritage. Adopting the concept of territory as base to understand the complexities in the city, this work clarifies and contextualizes the historical heritage legislation through the Master Plan and the City Statute. The work also focuses on the contribution of the geographer toward studies related to urban-environmental planning.

Transformação da paisagem da área de preservação ambiental APA no Município de Botucatu-SP

The Environmental Protection Areas (EPA) are a type of sustainable use conservation unit, therefore its territory have public and private areas. To establish and reorganize the use of EPA’s territory, aiming the environment conservation along with human actions, it is necessary a management plan that should be conducted within five years from the date of its creation. The EPA Botucatu, established in 1983, does not have its management plan completed until the present day. The consequences of lack of organization about the use and occupation of its territory can be observed in a comparative study of territorial classification between the years 1985 and 2010, which was the objective of this study. As a result, it could be observed the decrease of the remaining fragments and intense fragmentation of them, pointing to the need for projects that aim the restoration, conservation and connectivity between these fragments and the subsequent application

Estudo da viscosidade do sangue: quantidade de amostra necessária à obtenção de medida

Os valores encontrados para a viscosidade sanguínea tendem a aparecer com diferenças significativas, de acordo com o sexo, avançar da idade e uso de substâncias. Pelo fato de o sangue ser um fluido não newtoniano, não podemos expressar sua viscosidade em termos absolutos sem que sejam levadas em conta as condições em que a mensuração é feita. Estudos anteriores destacaram a importância esta medida, considerando o efeito da idade, o uso de substâncias, bem como sua relação com Fluxo Sanguíneo Regional Cerebral, o que pode indicar correlação com declínios cognitivos. Entretanto, a prática médica carece ainda de um método simples e clinicamente prático para a verificação desta medida, além de valores de referência. Como passo inicial no sentido de padronizar os procedimentos e condições do processo de medição, avaliamos a quantidade mínima de amostra necessária para a medição da viscosidade sanguínea, utilizando este equipamento. Um total de 20 amostras de sangue foram coletadas de indivíduos saudáveis, entre 18 e 60 anos, em tubos com EDTA, e cada amostra dividida em 9 sub-amostras (de 600μl, 550μl, 500μl, 450μl, 400μl, 350μl, 300μl, 250μl e 200μl), totalizando, portanto, um total de aproximadamente 180 medições. A quantidade de 200 μl apresentou diferenças significativas com relação à primeira medida obtida, quando comparada com todas as demais quantidades (p<0,001), o que indica que com o uso desta quantidade, não é possível obter resultados confiáveis, pois existe alteração nos valores de VS obtidos relacionada à quantidade de material utilizado. Considerando a menor quantidade, bem como, que estivesse dentro da faixa de variação aceitável de 0,2 mPa.s, e a qual não apresentasse diferença significativa quando comparadas às demais (p>0,05), foi verificada a quantidade de 250 μl como a mais eficiente... (Resumo completo, clicar acesso eletrônico abaixo)

O trabalho dos catadores de materiais recicláveis de Presidente Prudente, São Paulo: um estudo aplicado na COOPERLIX e na coleta seletiva municipal

This work focuses on analyzing the organization of the Workers Cooperative Recyclable Products of Presidente Prudente (COOPERLIX) and selective collection of municipal service. Aiming to analyze the issue of solid waste and the work of collectors of recyclable materials in an organized Economic Development Outreach (ESS). Throughout the work was carried out theoretical approaches on the issue of solid waste, the precarious work of scavengers, some laws, among others, were conducted several field studies over the years 2011 and 2012 in COOPERLIX, with the realization meetings and debates on various subjects; tools were also used Geographic Information Systems mainly in the production of selective collection of municipal map, now at 100% of the urban area of Presidente Prudente. Finally, we carried out a comprehensive analysis of the situation of the productive and financial COOPERLIX during those two years, checking their capability and weaknesses

Coleta e estratégia simplificada de tratamento de água de chuva para fins não potáveis com ênfase no uso industrial

Este projeto investigou alternativa de tratamento simplificada para fins não potáveis para aplicação em escala industrial. É neste contexto que esta pesquisa alojou sua importância – nas possíveis estratégias de tratamento, em especial na utilização de sistema contendo filtro de pressão e no uso do amido natural de milho como coagulante, auxiliando no processo de filtração, visando aumento da segurança sanitária e da qualidade da água de chuva disponibilizada para aproveitamento. A hipótese do trabalho foi investigar a possibilidade da diminuição ou até eliminação do volume de água descartada na operação de abstração da primeira chuva. Para tal, foram realizados ensaios em escala de laboratório com uma precipitação coletada nos dias 10/03/08 e 03/04/2008. Os resultados da estratégia de tratamento delineada foram comparados com os obtidos pela estratégia convencional com descarte de 1,5 mm da primeira chuva, o que corresponde a um volume de 558 L de água de chuva. Utilizou-se a dosagem de 6,0 mg/L para fim de comparação com a água bruta, sem nenhum tratamento, e com a água somente filtrada. Os resultados não confirmaram a hipótese de que o tratamento com amido natural permite eliminar a etapa de abstração da primeira chuva, porém, vale ressaltar que a qualidade da água coletada já era muito boa previamente ao tratamento. Merece destaque o fato de que a amostra de precipitação submetida aos ensaios apresentados foi coletada em período de freqüente precipitação e, embora tenha sido observado o interstício de 3 dias consecutivos de estiagem a qualidade da água de chuva coletada pode ser considerada boa (34UH de cor aparente; 8,84 UT de turbidez e 238,2 (NMP) de coliformes totais e 3 (NMP) de coliformes termotolerantes). Acredita-se que, para o caso de precipitações ocorridas após longo período de estiagem, a necessidade de tratamento... (Resumo completo, clicar acesso eletrônico abaixo)

Efeito das baixas concentrações de agentes antineoplásicos sobre linfócitos humanos de sangue periférico

Our previous studies have shown that low concentrations (noncytotoxics) of antineoplastic agents modulate positively the dendritic cells, favoring their in vitro maturation and improving their antigen presenting function. The effects on colorectal cancer cells (HCT-116) were also investigated and we have observed an increased immunogenicity and susceptibility to cytotoxic T cells. Thereby, this study aimed to investigate the effect of 5-fluorouracil (5-FU) an azacitidine (AZA), in minimum effective and noncytotoxic concentrations on lymphocytes of healthy donors. In this study we have analyzed the cytotoxic effect of drugs at these concentrations as well as the proliferative ability of lymphocytes. In vitro production of IL-10 and IFN-γ has been also evaluated. We have observed that low concentrations of those chemotherapeutic agents are not cytotoxic for lymphocytes. However, the minimum effective concentrations (5-FU: 0,410±0,088 e AZA: 0,757±0,233; p<0, 05) have reduced the cell number. Proliferative activity of allogeneic lymphocytes in a mixed reaction (MLR) was not affected by the treatment. The cytokine production was not affected by the treatments, either. In conclusion, low concentrations of 5-FU and AZA has no deleterious effects on human peripheral blood lymphocytes and seems to be safe for combinatory administration with DC vaccines