Effects of genetic diversity of grass on insect species diversity at higher trophic levels are not due to cascading diversity effects

Genetic diversity in plant populations has been shown to affect the species diversity of insects. In grasses, infection with fungal endophytes can also have strong effects on insects, potentially modifying the effects of plant genetic diversity. We manipulated the genetic diversity and endophyte infection of a grass in a field experiment. We show that diversity of primary parasitoids (3rd trophic level) and, especially, secondary parasitoids (4th trophic level) increases with grass genetic diversity while there was no effect of endophyte infection. The increase in insect diversity appeared to be due to a complementarity effect rather than a sampling effect. The higher parasitoid diversity could not be explained by a cascading diversity effect because herbivore diversity was not affected and the same herbivore species were present in all treatments. The effects on the higher trophic levels must therefore be due to a direct response to plant traits or mediated by effects on traits at intermediate trophic levels.

Decomposition Rates and Nitrogen Release of Turf Grass Clippings

Decomposition rates and N release patterns of turfgrass clippings from lawns are not well understood. Litter bags containing clippings were inserted into the thatch layer of a coolseason turf. The experiment was arranged as a 2 × 4 factorial in a randomized complete block design with three replicates. Treatments included four rates of N fertilizer (0, 98, 196, and 392 kg N ha-1 yr-1) and two clipping treatments (returned vs. removed). Litter bags were removed periodically over the growing season and samples were analyzed for biomass, N and C concentrations, and C:N ratio on an ash-free basis. Percentage N loss from the clippings after 16 weeks ranged from 88% to 93% at the 0 and 392 kg N ha-1 rates, respectively, and from 86% to 94% when clippings were removed (CRM) or returned (CRT), respectively. Percentage C loss from the clippings ranged from 94% to 95% at the 0 and 392 kg N ha-1 rates, respectively, and from 92% to 96% with CRM and CRT, respectively. Cumulative N release was similar across N fertilization rates, (ranging from 131 g N kg-1 to 135 g N kg-1 tissue) but was higher for CRT (151 g N kg-1 tissue) than for CRM (128 g N kg-1 tissue). Grass clippings decomposed rapidly and released N quickly when returned to the turf thatch layer. This indicates the potential for reduced N fertilization when clippings are returned. Such rapid decomposition also suggests that the contribution of grass clippings to thatch development is negligible.

Effects of salinity, temperature and cadmium stress on cadmium -binding protein in the grass shrimp, {\it Palaemonetes pugio}

The combined effects of salinity, temperature and cadmium stress on survival and adaptation through cadmium-binding protein (CdBP) accumulation were studied in the grass shrimp, Palaemonetes pugio. In 96-hour bioassays, shrimp were exposed to zero or one of three levels of cadmium, under one of six different salinity (15, 25, or 35$\perthous$) and temperature (20 or 30$\sp\circ$C) regimes. CdBP concentrations were quantified in survivors from the 24 exposure groups. Salinity and temperature did not affect survivorship unless the shrimp were also exposed to cadmium. Grass shrimp were most sensitive to cadmium at low salinity-high temperature, and least sensitive at high salinity-low temperature. The incidence of cadmium-associated black lesions in gill tissue was influenced by salinity and temperature stress. P. pugio produced a 10,000 dalton metallothionein-like CdBP when exposed to at least 0.1 mg Cd$\sp{2+}$/L for 96 hours. Accumulation of CdBP was increased with increases in the exposure cadmium level, increases in temperature and decreases in salinity, independently and in conjunction with one another. Maximum CdBP concentrations occurred in grass shrimp that survived the salinity-temperature-cadmium conditions creating maximum stress as measured by highest mortality, not necessarily in shrimp exposed to the highest cadmium levels. The potential utility of this method as a monitor of physiological stress in estuarine biota inhabiting metal-polluted environments is discussed. ^

Soybean Yield Response to Rhizobium Inoculation on Converted Grass Pasture

Much of the soybean plant's nitrogen requirement is supplied through nitrogen fixation when atmospheric nitrogen is converted into a usable form for the plant. Nitrogen fixation is critical for producing higher yield in soybean. For nitrogen fixation to occur, nitrogen-fixing bacteria (genus Rhizobium) need to be present in the soil. If soils do not already contain a high population of Rhizobium, these bacteria can be added either as a liquid or granular peat inoculant, or as a peat-based powder. The different forms can be seed applied or used in-furrow.

Uso innovador de un SIG en la logística de productos forestales

El costo del transporte de los productos forestales reviste gran importancia en la rentabilidad de la actividad, incidiendo directamente en el precio percibido por el productor. Ante esta situación sería de utilidad, para el productor y para el transportista, conocer cuál sería el recorrido óptimo desde el lugar de partida (ej. lote del productor) hasta el lugar de destino (ej. acopiador). En consecuencia, se propone comprobar la factibilidad de utilizar el entorno de los Sistemas de Información Geográfica (SIG) en la determinación, de manera rápida y con exactitud, del camino óptimo entre un lote forestal y el aserradero.

Los sistemas de información geográficos -SIG- en la enseñanza de la geografía : una experiencia didáctica

Fil: Zilio, Cecilia Karina. Universidad Nacional de La Plata. Facultad de Humanidades y Ciencias de la Educación; Argentina.

Enseñanza de la geografía e Informática : El uso del SIG en una experiencia pedagógica innovadora

El presente trabajo da cuenta de una experiencia pedagógica innovadora realizada en el marco del Convenio DINIECE, MECYT - FAHCE, UNLP. Plantea la utilización de los Sistemas de Información Geográfica (SIG) con fines pedagógicos. Dicha experiencia se llevó a cabo en el curso "Seminario de Itinerarios Urbanos" en el Colegio Nacional "Rafael Hernández" de la UNLP. Partiendo de una concepción crítica desde la Geografía y desde la Didáctica, se incentivó a los alumnos/as a comprender la realidad socio-ambiental en la cual están inmersos. Para ello se identificaron, desde el análisis territorial y espacial, problemáticas ambientales en el partido de La Plata. Los alumnos/as vivenciaron y experimentaron una forma de trabajo en la que la innovación estuvo dada por la utilización de nuevas tecnologías -SIG- en consonancia con una metodología didáctica que fomenta aprendizajes significativos.

Enseñanza de la geografía e Informática : El uso del SIG en una experiencia pedagógica innovadora

El presente trabajo da cuenta de una experiencia pedagógica innovadora realizada en el marco del Convenio DINIECE, MECYT - FAHCE, UNLP. Plantea la utilización de los Sistemas de Información Geográfica (SIG) con fines pedagógicos. Dicha experiencia se llevó a cabo en el curso "Seminario de Itinerarios Urbanos" en el Colegio Nacional "Rafael Hernández" de la UNLP. Partiendo de una concepción crítica desde la Geografía y desde la Didáctica, se incentivó a los alumnos/as a comprender la realidad socio-ambiental en la cual están inmersos. Para ello se identificaron, desde el análisis territorial y espacial, problemáticas ambientales en el partido de La Plata. Los alumnos/as vivenciaron y experimentaron una forma de trabajo en la que la innovación estuvo dada por la utilización de nuevas tecnologías -SIG- en consonancia con una metodología didáctica que fomenta aprendizajes significativos.

Los sistemas de información geográficos -SIG- en la enseñanza de la geografía : una experiencia didáctica

Fil: Zilio, Cecilia Karina. Universidad Nacional de La Plata. Facultad de Humanidades y Ciencias de la Educación; Argentina.

Enseñanza de la geografía e Informática : El uso del SIG en una experiencia pedagógica innovadora

El presente trabajo da cuenta de una experiencia pedagógica innovadora realizada en el marco del Convenio DINIECE, MECYT - FAHCE, UNLP. Plantea la utilización de los Sistemas de Información Geográfica (SIG) con fines pedagógicos. Dicha experiencia se llevó a cabo en el curso "Seminario de Itinerarios Urbanos" en el Colegio Nacional "Rafael Hernández" de la UNLP. Partiendo de una concepción crítica desde la Geografía y desde la Didáctica, se incentivó a los alumnos/as a comprender la realidad socio-ambiental en la cual están inmersos. Para ello se identificaron, desde el análisis territorial y espacial, problemáticas ambientales en el partido de La Plata. Los alumnos/as vivenciaron y experimentaron una forma de trabajo en la que la innovación estuvo dada por la utilización de nuevas tecnologías -SIG- en consonancia con una metodología didáctica que fomenta aprendizajes significativos.

Los sistemas de información geográficos -SIG- en la enseñanza de la geografía : una experiencia didáctica

Fil: Zappettini, María Cecilia. Universidad Nacional de La Plata. Facultad de Humanidades y Ciencias de la Educación; Argentina.

SIG y Visor Web de energía solar y eólica. Aplicación práctica en el País Vasco.

El proyecto es un Sistema de Información Geográfica y Visor web centrado en las energías renovables solar y eólica, contiene funcionalidad dirigida a facilitar elacceso de los ciudadanos y de las empresas del sector a la información de estas dos energías. Incorpora una herramienta de edición que pretende tener una base de datos actualizada de los parques solares y eólicos de España. Otra orientada al entorno urbano que permite saber la electricidad aproximada que se generaría en la cubierta de un edificio de Vitoria en función del panel solar que se instale en dicha cubierta. También se ha realizado un análisis espacial para encontrar los lugares óptimos para la instalación de paneles solares y eólicos en el País Vasco, se han publicado las capas resultado en el visor para que puedan acceder a ellas cualquier sociedad o empresa que le interese conocer este tipo de información. Para estos análisis se han tenido en cuenta estudios de diferentes universidades e informes de organizaciones como Greenpeace. No obstante, no deja de ser una propuesta objeto de posibles mejoras.

Validation of microsatellite markers for cytotype discrimination in the model grass Brachypodium distachyon

Brachypodium distachyon (2n = 2x = 10) is a small annual grass species where the existence of three different cytotypes (10, 20 and 30 chromosomes) has long been regarded as a case of autopolyploid series, with x = 5. However, it has been demonstrated that the cytotypes assumed to be polyploids represent two separate Brachypodium species recently named as B. stacei (2n = 2x = 20) and B. hybridum (2n = 4x = 30). The aim of this study was to find a PCR-based alternative approach that could replace standard cytotyping methods (i. e., chromosome counting and flow cytometry) to characterize each of the three Brachypodium species. We have analyzed with four microsatellite (SSR) markers eighty-three Brachypodium distachyon-type lines from varied locations in Spain, including the Balearic and Canary Islands. Within this set of lines, 64, 4 and 15 had 10, 20 and 30 chromosomes, respectively. The surveyed markers produced cytotype-specific SSR profiles. So, a single amplification product was generated in the diploid samples, with non-overlapping allelic ranges between the 2n = 10 and 2n = 20 cytotypes, whereas two bands, one in the size range of each of the diploid cytotypes, were amplified in the 2n = 30 lines. Furthermore, the remarkable size difference obtained with the SSR ALB165 allowed the identification of the Brachypodium species by simple agarose gel electrophoresis.

Ground cover and leaf area index relationship in a grass, legume and crucifer crop

Canopy characterization is essential for describing the interaction of a crop with its environment. The goal of this work was to determine the relationship between leaf area index (LAI) and ground cover (GC) in a grass, a legume and a crucifer crop, and to assess the feasibility of using these relationships as well as LAI-2000 readings to estimate LAI. Twelve plots were sown with either barley (Hordeum vulgare L.), vetch (Vicia sativa L.), or rape (Brassica napus L.). On 10 sampling dates the LAI (both direct and LAI-2000 estimations), fraction intercepted of photosynthetically active radiation (FIPAR) and GC were measured. Linear and quadratic models fitted to the relationship between the GC and LAI for all of the crops, but they reached a plateau in the grass when the LAI mayor que 4. Before reaching full cover, the slope of the linear relationship between both variables was within the range of 0.025 to 0.030. The LAI-2000 readings were linearly correlated with the LAI but they tended to overestimation. Corrections based on the clumping effect reduced the root mean square error of the estimated LAI from the LAI-2000 readings from 1.2 to less than 0.50 for the crucifer and the legume, but were not effective for barley.