Phyllosticta citricarpa: diversidade genética temporal em pomares de citrus sinensis e sensibilidade a fungicidas

Pós-graduação em Agronomia (Genética e Melhoramento de Plantas) - FCAV

Dispositivo microfluídico de borracha natural (LAB-ON-A-CHIP)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Fenotipagem para estudos de estudos de tolerância à deficiência hídrica em eucalipto

Pós-graduação em Agronomia (Genética e Melhoramento de Plantas) - FCAV

Análise histológica e histomorfométrica do processo de reparo de defeitos ósseos em ratos diabéticos preenchidos com osso autógeno e recobertos por membrana de matriz óssea homógena ou por membrana de politetrafluoretileno

Pós-graduação em Odontologia - FOA

Estudos de permeação e liberação de peptídeos antimicrobianos estruturalmente derivados da toxina CCDB

O uso de peptídeos sintéticos para o desenvolvimento de novas drogas é uma estratégia promissora no campo da biotecnologia. Peptídeos derivados de toxinas bacterianas intracelulares, produzidas por sistemas de morte pós-segregacional (PKS) tais como CcdB e ParE são exemplos dessa estratégia. Porém, moléculas com estrutura peptídica derivadas de toxinas bacterianas apresentam sérios problemas na aplicação terapêutica por apresentarem baixa solubilidade e difícil permeabilidade em membranas bacterianas. O objetivo desse estudo consistiu no desenvolvimento e aprimoramento de sistemas nanoestruturados (lipossomas) que permita a imobilização de análogos peptídicos da toxina CcdB e sua consequente translocação no citosol bacteriano, permitindo que os mesmos atinjam seus alvos celulares, enzimas DNA girase e Topoisomerase IV. Lipossomas do tipo SUV (small unilamellar vesicles), foram preparados pela técnica de extrusão-evaporação variando-se suas formulações. Desta forma, pretendeu-se avaliar a eficiência de encapsulação dos peptídeos através de técnicas de cromatografia líquida de alta eficiência (CLAE) e espectroscopia de UV-Vis e fluorescência. Após testes de eficiência de encapsulação, os lipossomas contendo os análogos peptídicos encapsulados, foram submetidos a ensaio de inibição de crescimento em meio líquido para duas espécies bacterianas: Staphylococcus aureus e Escherichia coli. Resultados demonstraram que a utilização de sistemas nanoestruturados é de grande importância para viabilizar a aplicação desta classe de biomoléculas em estudos terapêuticos, permitindo assim, que tais peptídeos possam ser utilizados como antibióticos promissores, se associados a sistemas de transporte e liberação controlada de moléculas peptídicas.

Desenvolvimento de um novo sistema dinâmico para avaliação da liberação de fármacos

Pós-graduação em Biociências - FCLAS

Funcionalização de celulose bacteriana com peptídeo RGD para reparação tecidual de pele

Pós-graduação em Química - IQ

Síntese e estudos de sistemas de bioencapsulação associados a peptídeos antimicrobianos miméticos da toxina natural CcdB

Pós-graduação em Biotecnologia - IQ

Aplicação da membrana de látex natural (NRL) como suporte para cultura das células osteogênicas MC3T3-E1 para regeneração óssea guiada (GBR)

Pós-graduação em Biotecnologia - IQ

Tratamiento de maloclusión classe II división 1 con el aparato de Herbst tipo férula, después del pico de crecimiento puberal

Malocclusion class II-1, is represented by a high prevalence in Brazil, being something common in orthodontic practice. One of the main characteristics of this malocclusion is jaw retrusion, by what many devices of jaw advance are presented in the literature. Being one of them Herbst's device, which is a functional and fixed device created by Emil Herbst (1905) and updated by Hans Pancherz in the decade of 80s.This device is characterized by keeping the jaw advance in a continuous way, while presenting a less active treatment, leading to an immediate aesthetic impact, and the patient cooperation is not required. To improve the anchoring and prevent the collapse of the apparatus was set up last amended version of it by Dr Raveli quoted as Herbst splint. Recent research indicates the use of this device after the peak pubertal growth, creating an appropriate response condylar. The aim of this work is to show the orthodontist a choice of how to use the splint Herbst in Class malocclusions II-1.

Efeitos da aplicação de resina hidrofóbica sobre a resistência de união de adesivos autocondicionantes de frasco único

Introduction: It is known that self-etching adhesive systems can act as semi-permeable membranes. Objective: Evaluate the effects of additional layer of hydrophobic resin on the microtensile bond strength of self-etching one-bottle adhesives. Material and method: Sixty bovine incisors were used in this study. The facial enamel surfaces of the crowns were abraded with silicon carbide paper to expose flat, mid-coronal dentin surfaces. The following adhesives were used: Clearfil Tri S Bond (CTSB), AdheSE One (ADH) and One Coat  7.0 (OC). Each material was tested with and without applying an additional layer of hydrophobic material from the same manufacturer. Z-350 composite resin was inserted in three 1 mm increments. All adhesive restorative procedure was performed under simulated pulpal pressure and the microtensile test was performed immediately after curing the composite resin. Data were submitted to ANOVA and Tukey test (p < 0.05). Result: For all adhesives tested, the worst results were observed in groups which the additional layer of hydrophobic resin was not applied. Conclusion: The application of additional layer of hydrophobic material can improve the adhesion of self-etching all-in-one adhesive systems.

Criopreservação de oócitos de Prochilodus lineatus (Valenciennes, 1836) (Teleostei, Prochilodontidae)

Pós-graduação em Ciência e Tecnologia Animal - FEIS

Estudo de interação entre um hormônio estrógeno e modelos simples de membrana biológica

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Estocagem tecidual e utilização de lipídeos em Matrinxã, Brycon cephalus (Günther, 1869)

Lipids have important biological functions, suchas membrane constituents, hormone precursors, and are efficient energy reserves, due to thers high caloric content. In fish, but not in mammals, lipid storage patterns are quite diverse. The aim of this study was to determine lipid distribution in somatic and reproductive tissues of matrinxã, Brycon cephalus, both male and female, highlighting somatic indices related to lipid dynamics. From Oct 98 to Jan 99, a total of 174 fish (8-12 each month) were sampled. After fish anesthesia, heparinized blood was collected for plasma triacylglycerol determination. Fish were individually weighed and measured, and liver, gonads and visceral fat were collected and weighed for HSI (hepatosomatic index), GSI (gonad somatic index) and MFI (mesenteric fat index) calculation. The highest values of total lipids were found in red muscle (about 18%), liver and gonads (about 16.5%). The white muscle had a lower concentration (2.5%). Analyses of variance of HSI and MFI showed fluctuations along the experimental period with lowest concentrations in the periods of highest temperatures. Matrinxã store lipids in several body tissues, including mesenteric fat, liver, muscles and gonads.

Impaired protamination and sperm DNA damage in a Nellore bull with high percentages of morphological sperm defects in comparison to normospermic bulls

The routine semen evaluation assessing sperm concentration, motility and morphology, does not identify subtle defects in sperm chromatin architecture. Bulls appear to have stable chromatin, with low levels of DNA fragmentation. However, the nature of fragmentation and its impact on fertility remain unclear and there are no detailed reports characterizing the DNA organization and damage in this species. The intensive genetic selection, the use of artificial insemination and in vitro embryo production associated to the cryopreservation process can contribute to the chromatin damage and highlights the importance of sperm DNA integrity for the success of these technologies. Frozen-thawed semen samples from three ejaculates from a Nellore bull showed high levels of morphological sperm abnormalities (55.8±5.1%), and were selected for complementary tests. Damage of acrosomal (76.9±8.9%) and plasma membranes (75.7±9.3%) as well as sperm DNA strand breaks (13.8±9.5%) and protamination deficiency (3.7±0.6%) were significantly higher compared to the values measured in the semen of five Nellore bulls with normospermia (24.3±3.3%; 24.5±6.1%; 0.6±0.5%; 0.4±0.6% for acrosome, plasma membrane, DNA breaks and protamine deficiency, respectively) (P<0.05). Motility and percentage of spermatozoa with low mitochondrial potential showed no differences between groups. This study shows how routine semen analyses (in this case morphology) may point to the length and complexity of sperm cell damage emphasizing the importance of sperm function testing.