Antiproliferative effect of Pterogyne nitens on melanoma cells

As part of our program of bioprospecting for novel antitumor drug prototypes, twenty extracts and fractions obtained from Pterogyne nitens Tul. (Fabaceae, Caesalpinioideae) were screened for antiproliferative activity against B16F10 murine melanoma cells, by the MTT colorimetric assay. The strongest activity was found in EtOAc fractions from the flowers (IC50 = 0.35 ��g/mL), fruits (IC50 = 0.34 ��g/mL), leaves (IC50 = 0.33 ��g/mL) and stems (IC50 = 0.29 ��g/mL). Analysis by TLC and HPLC-DAD showed the presence of guanidine alkaloids, flavones and flavonols in the bioactive samples. Additionally, a phytochemical study of the EtOAc fraction of the stems afforded quercetin (1) and isoquercitrin (2), two flavonols with antiproliferative activity previously described in the literature. On the basis of these results, it can be concluded that P. nitens inhibits the growth of melanoma cells in vitro. Further investigations will be needed to assess the usefulness of the samples under study for the treatment of neoplasms and to characterize other bioactive compounds. Keywords: antiproliferative; Pterogyne nitens; Caesalpinioideae; melanoma; flavonoids; Fabaceae.

Diagn��stico popular e incid��ncia de doen��as e males tratados pela fitoterapia tradicional

Este trabalho analisa as doen��as diagnosticadas pelo povo, as plantas medicinais empregadas em sua cura, os rem��dios utilizados, a medicina religiosa do candombl��, umbanda e catimb�� e a fitoterapia no passado.

A l��gica simb��lica na medicina popular

Trabalho explorat��rio visando detectar a exist��ncia de uma l��gica simb��lica na medicina popular (fitoterapia).

Efeito do (-)-mentol sobre a ��lcera g��strica: estudo do mecanismo gastroprotetor

P��s-gradua����o em Ci��ncias Biol��gicas (Farmacologia) - IBB

Influ��ncia do extrato de ch�� verde na remodela����o card��aca ap��s o infarto agudo do mioc��rdio

P��s-gradua����o em Fisiopatologia em Cl��nica M��dica - FMB

Obten����o de nanopart��culas lip��dicas s��lidas contendo (-)-Terpinen-4-Ol

Coordena����o de Aperfei��oamento de Pessoal de N��vel Superior (CAPES)

A����o moduladora do cortisol e da Aloe vera no sistema imune inato de peixes

P��s-gradua����o em Aquicultura - FCAV

Avalia����o do efeito do alho (Allium sativum L.) sobre o colesterol plasm��tico em coelhos com hipercolesterolemia induzida

The use of plants to treat diseases or even to cure them is a high diffused popular tradition, and several studies discuss the therapeutic and pharmacological properties of garlic in the reduction of hyperlipidaemias. The objective of the present study was to investigate the influence of the aqueous extract of garlic (Allium sativum) to treat the serum cholesterol of rabbits with experimental hypercholesterolemia. The animals were divided into G1 (control group) and G2 (group treated with garlic). The experiment was developed according to 3 phases: during the 1st phase, all animals were provided with a regular diet to evaluate the basal cholesterol; during the 2nd phase, all animals received a supplemented diet until the end of the experiment, in order to develop hypercholesterolemia and, in the 3rd phase, the animals on G2 received the garlic treatment. The cholesterol registered on the 1st phase was 39.94 �� 9.57 mg dL-1. On the 2nd phase, there was an increase on the serum cholesterol level in both groups - higher than 100 mg dL-1. Concerning the treatment, the garlic did not reduce the serum cholesterol in rabbits.

Influ��ncia da aduba����o com nitrog��nio e f��sforo na produ����o de Jambu, Acmella oleracea (L) R.K. Jansen

The objective of this work was to evaluate the influence of the use of nitrogen and phosphorus in the culture of jambu. The experiment was initiated at the experimental farm of the agency APTA Polo Vale do Ribeira, using the Nazar�� cultivar. The experimental design was complete randomized with fourlevelsof nitrogen (0, 37.5, 75 and 112.5 kg ha-1 N) and phosphorus (0, 75, 150 and 300 Kg ha-1P2O5),and four reapplications. After soil preparation, liming and fertilization, seedlings of jambu were transplanted at a spacing of 0.50 x 0.5 m. The experimental plot was 1.0 x 1.0 m. Fresh and dry mass of leaves and flowers and number of flowers were analyzed. The production of fresh and dry leaves and flowers were influenced by fertilization. Thelevelsof nitrogen affected the outcome, as theyprovided a linear increase in all variables, and the phosphorus level of 75 kgha-1 P2O5 caused the highest average production in the number of flowers and in the fresh and dried mass of flowers.

Supplementation and therapeutic use of vitamin D in patients with multiple sclerosis: Consensus of the Scientific Department of Neuroimmunology of the Brazilian Academy of Neurology

A esclerose m��ltipla (EM) �� uma doen��a inflamat��ria, autoimune, desmielinizante e degenerativa do sistema nervoso central. Estudos epidemiol��gicos t��m identificado associa����es de hipovitaminose D com doen��as autoimunes. O principal objetivo desta revis��o �� responder se h�� evid��ncias que indiquem o uso terapêutico de vitamina D em monoterapia para pacientes com EM. Por meio dos sites PUBMED, EMBASE, LILACS e Scielo foram realizadas buscas usando os descritores ���vitamin D���, e ���multiple sclerosis��� at�� 12/09/2013. Estudos cl��nicos randomizados, controlados e duplo-cegos foram selecionados para avaliar a resposta terap��utica da vitamina D na EM. N��o foram encontradas evid��ncias cient��ficas que justifiquem o uso da vitamina D em monoterapia no tratamento da EM, na pr��tica cl��nica.

Physiological indexese macro- and micronutrients in plant tissue and essential oil of Mentha piperita L. grown in nutrient solution with variation in N, P, K and Mg levels

Mentha piperita L. is an aromatic and medicinal species of the family Lamiaceae, known as mint or peppermint, and its leaves and branches produce essential oil rich in menthol. This study aimed to evaluate physiological indexes, macro- and micronutrients inthe shootsand essential oil of Mentha piperita L. grown in nutrient solution number 2 of Hoagland and Arnon (1950) with different N, P, K and Mg levels. Shoot length, dry mass of the different organs, total dry mass, leaf area, essential oil yield and composition, and macronutrient (N, P, K, Mg, Ca, S) and micronutrient (Mn, Cu, Fe, Zn) contents in the shoot were evaluated. Plants treated with 65%N/50%P/25%K/100%Mg had a tendency towards longer shoot, greaterroot and leaf blade dry masses, higher essential oil yield, higher menthol levels and lower menthone levels. The results showed that Mentha can be grown in nutrient solution by reducing 65% N, 50% P, 25% K and 100% Mg. This solution had better development compared to the other tested treatments. Therefore,we recommendMentha piperita L. to be grown with such nutrient levels.

Avalia����o dos mecanismos de a����o envolvidos na atividade antiulcerog��nica de Serjania marginata frente �� doen��a ulcerosa p��ptica experimental

P��s-gradua����o em Ci��ncias Biol��gicas (Farmacologia) - IBB

Potencial da pr��polis: para o desenvolvimento de novas drogas

P��s-gradua����o em Biologia Geral e Aplicada - IBB

Incid��ncia de fungos e germina����o de sementes de feij��o-caupi (Vigna unguiculata L. (Walp) tratadas com ��leo de nim (Azadirachta indica A. Juss)

Funda����o de Amparo �� Pesquisa do Estado de S��o Paulo (FAPESP)

Atividade antibacteriana de ��leos Essenciais sobre Streptococcus mutans e Staphylococcus aureus

Funda����o de Amparo �� Pesquisa do Estado de S��o Paulo (FAPESP)