Dynamics of the chemical composition and productivity of composts for the cultivation of Agaricus bisporus strains

Two compost formulations based on oat straw (Avena sativa) and brachiaria (Brachiaria sp.) were tested for the cultivation of three Agaricus bisporus strains (ABI-07/06, ABI-05/03, and PB-1). The experimental design was a 2 x 3 factorial scheme (composts x strains) with 6 treatments and 8 repetitions (boxes containing 12 kg of compost). The chemical characterization of the compost (humidity, organic matter, carbon, nitrogen, pH, raw protein, ethereal extract, fibers, ash, cellulose, hemicellulose, and lignin) before and after the cultivation of A. bisporus and the production (basidiomata mass, productivity, and biological efficiency) were evaluated. Data were submitted to variance analysis, and averages were compared by means of the Tukey's test. According to the results obtained, the chemical and production characteristics showed that the best performances for the cultivation of A. bisporus were presented by the compost based on oat and the strain ABI-07/06.

Isolamento e seleção de fungos filamentos produtores de xilanases e celulases utilizando resíduos agroindustriais

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Aplicação de corretivos de acidez do solo na implantação do sistema plantio direto

Pós-graduação em Agronomia (Agricultura) - FCA

Produção de biossurfactantes utilizando resíduos agroindustriais como substrato

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Desenvolvimento de um biorreator rotativo para produção de enzimas celulolíticas por fermentação em estado sólido

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Isolamento e seleção de fungos filamentosos termofílicos/termotolerantes produtores de celulases e xilanases e aplicação dos extratos enzimáticos na sacarificação do bagaço de cana-de-açúcar

Pós-graduação em Microbiologia - IBILCE

Produção de ligno-hemi-celulases por fermentação em estado sólido e avaliação dos efeitos da aplicação das enzimas na composição química e estrutura do bagaço e da palha de cana

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Feed type for induced molting of commercial layer hens

An experiment employing three hundred and twenty 81-week-old Lohmann LSL commercial-breed hens was conducted to compare alternative induced-molting methods with the conventional method (fasting). Induced molting lasted 28 days at most, production and quality being monitored for four periods of 28 days thereafter. A completely randomized experimental design with five treatments, eight replicates of eight birds each per plot was adopted. The following experimental treatments were applied until a loss of 26% of body weight was reached: T1 - fasting, T2 - wheat bran ad libitum, T3 - rice bran ad libitum, T4 - cracked rice ad libitum, T5 - ground alfalfa ad libitum. Birds were then fed production diet ad libitum, except for those on treatment T1 (fasting) which received 30, 60 and 100 g/bird/day and then feed ad libitum. During induced molting the birds were exposed to a natural photoperiod and at day 28 that period was increased by 30 minutes/week until reaching 16 hours of light/day. The characteristics evaluated during induced molting were: feed intake, body weight changes and laying percentage. In the post-molt period, performance (feed intake, laying percentage, egg weight, egg mass, feed conversion ratio per dozen and per egg mass and percentage of broken eggs) and egg quality (specific gravity, eggshell breaking strength, percentages of eggshell, yolk, and albumen, eggshell thickness, yolk color and Haugh unit) were evaluated. Every 28 days one egg was collected from each repetition for three consecutive days for quality assessment. The use of rice bran and wheat bran is viable as molting inducers since the birds given those treatments display performance and egg quality similar to those fasted during the induced molting and also because these ingredients promote easier handling, eliminates the need for grinding and feed-mixing equipment and, being less aggressive, provide greater bird welfare.

Teores e estoque de carbono em frações lábeis e recalcitrantes da matéria orgânica do solo sob integração lavoura-pecuária no bioma Cerrado

The study of organic matter and its compartments and their relationship with management, aims to develop strategies for increasing their levels in soils and better understanding of its dynamics. This work aimed to evaluate the fractions of soil organic matter and their carbon stocks in different soil cover system in crop-livestock integration and native Cerrado vegetation. The study was conducted at the farm Cabeceira, Maracaju-MS, sample area have the following history: soybean/corn + brachiaria/cotton/oat + pasture/soybean/formation of pasture/grazing, sampling was carried out in two seasons, dry (May/2009) and rainy (March 2010), in the dry season, crops present were: pasture, corn and cotton + brachiaria and in the rainy season were corn, cotton and soybeans, so the areas in the two evaluation periods were: pasture / maize + brachiaria / cotton, cotton / soybean area and a native of Savanna. Was performed to determine the exchangeable cations, particle size analysis, bulk density, organic carbon, particle size fractionation of organic matter of the soil with the quantification of particulate organic carbon (POC) and organic carbon associated with minerals (OCam). Was also quantified the carbon stock and size fractions. The area of pasture / maize showed higher carbon stock in the particulate fraction in the topsoil. The area of cotton / soy due to its lower clay, showed the greatest loss of carbon. Because of the areas have the same history, the stock of more recalcitrant fraction was not sensitive to variations in coverage. The POC fraction appears more sensitive to different soil covers and seasonality.

Plant growth promoting bacteria in Brachiaria brizantha

Brachiaria brizantha is considered one of the preferred fodders among farmers for having high forage yield and large production of root mass. The association of beneficial bacteria with these grasses can be very valuable in the recovery of the pasture areas with nutritional deficiency. With the aim of studying this possibility, we carried out the sampling of soil and roots of B. brizantha in three areas (Nova Odessa-SP, So Carlos-SP and Campo Verde-MT, Brazil). Seventy-two bacterial strains were isolated and used in tests to evaluate their biotechnological potential. Almost all isolates presented at least one positive feature. Sixty-eight isolates produced analogues of indole-3-acetic acid, ten showed nitrogenase activity when subjected to the method of increasing the concentration of total nitrogen (total N) in the culture medium and sixty-five isolates showed nitrogenase activity when subjected to acetylene reduction technique. The partial sequencing of 16S rRNA of these isolates allowed the identification of seven main groups, with the prevalence of those affiliated to the genus Stenotrophomonas (69 %). At the end, this work elected the strains C4 (Pseudomonadaceae) and C7 (Rhodospirillaceae) as promising organisms for the development of inoculants due to their higher nitrogenase activity.

Heavy Metals Extracted by DTPA and Organic Acids from Soil Amended with Urban or Industrial Residues

Understanding soil responses to the application of urban or industrial residues is necessary. In the current experiment, both sources of residues were applied to the soil surface of a Hapludox soil for 8 years. The four residues studied were biodigested and centrifuged sewage sludge (LB and LC, respectively), steel slag from the metallurgic industry (E), and limed mud from the pulp and cellulose industry (LCal). The residues were applied at 0, 2, 4, and 8 Mg ha(-1) four times from 2002 to 2010 when soybean was cultivated in the summer and lopsided oat and sorghum were cultivated during the winter. The contents of heavy metals were evaluated with diethylenetriaminepentaacetic acid (DTPA) and organic acids. The LB was the residue that increased the contents of soil micronutrients and heavy metals to their greatest levels, although within limits that impair the risks of environmental contamination.

Uso de resíduos de madeiras e frutos da amazônia para o cultivo in vitro do cogumelo comestível shiitake

Local abundant residues with low or without commercial value with potential to make the production of edible mushrooms economically viable in any region of Brazil must be employed for cultivation. Thus, the radial mycelial growth of LED 20 strain of Lentinula edodes (Berk.) Pegler was verified in culture media prepared with extract of regional residues by using substrates based on Protium puncticulatum, Cariniana micrantha and Caryocar glabum sawdust, supplemented with 20% of grinded residues from the barks of the fruits of Astrocaryum aculeatum and Theobroma grandiflorum. Residues from eucalyptus (Eucalyptus spp) sawdust and wheat bran (Triticum aestivum L) were used as witness because they are commonly used in the cultivation of L. edodes. The experimental design used was totally randomized, in 4x3 factorial scheme, totalizing 12 treatments with five repetitions, being that each repetition corresponded to a Petri dish, adding up a total of 60 dishes, which were incubated under 25 degrees C. The diameter of the colony was daily evaluated during seven days of incubation. After that period, it was verified that media prepared with P. puncticulatum extract, whose supplementation with both brans was favorable for fungic development, presented some of the highest averages of mycelial growth of LED 20 strain of L. edodes, confirming the potential of those residues.

Xylanase and beta-Xylosidase from Penicillium janczewskii: Production, Physico-chemical Properties, and Application of the Crude Extract to Pulp Biobleaching

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Production and Characterization of a Milk-clotting Protease Produced in Submerged Fermentation by the Thermophilic Fungus Thermomucor indicae-seudaticae N31

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Effect of gamma irradiation on the nutritional quality of Agaricus bisporus strains cultivated in different composts

The effect of irradiation doses (0, 125, 250 and 500 Gy) on the nutritional quality of A. bisporus mushrooms (strains ABI-07/06, ABI-05/03 and PB-1) cultivated in composts based on oat straw (Avena sativa) and brachiaria (Brachiaria sp.) was evaluated. The experimental design was 4 x 3 x 2 factorial scheme (irradiation doses x strains x composts), with 24 treatments, consisting of two repetitions each, totaling 48 experimental units (samples of mushrooms). The samples were irradiated in Cobalt-60 irradiator, model Gammacell 220 kGy, with dose rate of 0.740 kGy h(-1), according to the treatments proposed. Subsequently, the control (unirradiated) and the other treatments were maintained at 4 +/- 1 degrees C and 90% RH in a climatic chamber for carrying out the chemical analysis of the mushrooms on the 1st and 14th day of storage. It was found that all A. bisporus strains evaluated were food with excellent nutritional value, because they presented high protein and fiber contents and low ethereal extract content; the chemical characterization of the mushrooms was influenced by the compost type in which they were cultivated; gamma irradiation influenced the chemical composition of mushrooms.