The ATPase and protease domains of yeast mitochondrial Lon: Roles in proteolysis and respiration-dependent growth

The ATP-dependent Lon protease of Saccharomyces cerevisiae mitochondria is required for selective proteolysis in the matrix, maintenance of mitochondrial DNA, and respiration-dependent growth. Lon may also possess a chaperone-like function that facilitates protein degradation and protein-complex assembly. To understand the influence of Lon’s ATPase and protease activities on these functions, we examined several Lon mutants for their ability to complement defects of Lon-deleted yeast cells. We also developed a rapid procedure for purifying yeast Lon to homogeneity to study the enzyme’s activities and oligomeric state. A point mutation in either the ATPase or the protease site strongly inhibited the corresponding activity of the pure protein but did not alter the protein’s oligomerization; when expressed at normal low levels neither of these mutant enzymes supported respiration-dependent growth of Lon-deleted cells. When the ATPase- or the protease-containing regions of Lon were expressed as separate truncated proteins, neither could support respiration-dependent growth of Lon-deleted cells; however, coexpression of these two separated regions sustained wild-type growth. These results suggest that yeast Lon contains two catalytic domains that can interact with one another even as separate proteins, and that both are essential for the different functions of Lon.

Hepatitis B virus HBx protein deregulates cell cycle checkpoint controls.

The human hepatitis B virus (HBV) HBx protein is a small transcriptional activator that is essential for virus infection. HBx is thought to be involved in viral hepatocarcinogenesis because it promotes tumorigenesis in transgenic mice. HBx activates the RAS-RAF-mitogen-activated protein (MAP) kinase signaling cascade, through which it activates transcription factors AP-1 and NF-kappa B, and stimulates cell DNA synthesis. We show that HBx stimulates cell cycle progression, shortening the emergence of cells from quiescence (G0) and entry into S phase by at least 12 h, and accelerating transit through checkpoint controls at G0/G1 and G2/M. Compared with serum stimulation, HBx was found to strongly increase the rate and level of activation of the cyclin-dependent kinases CDK2 and CDC2, and their respective active association with cyclins E and A or cyclin B. HBx is also shown to override or greatly reduce serum dependence for cell cycle activation. Both HBx and serum were found to require activation of RAS to stimulate cell cycling, but only HBx could shorten checkpoint intervals. HBx therefore stimulates cell proliferation by activating RAS and a second unknown effector, which may be related to its reported ability to induce prolonged activation of JUN or to interact with cellular p53 protein. These data suggest a molecular mechanism by which HBx likely contributes to viral carcinogenesis. By deregulating checkpoint controls, HBx could participate in the selection of cells that are genetically unstable, some of which would accumulate unrepaired transforming mutations.

Hacer… hablar; hablar… tal vez habitar. Apuntes sobre lo dicho y hecho por Adolf Loos (1870-1933)

Ornamento y delito y Arquitectura, los dos artículos que sin duda han contribuido a difundir el ideario y a sustentar la celebridad del arquitecto Adolf Loos (1870-1933), proceden de sendas conferencias. Su origen, por consiguiente, es de naturaleza oral. Y la polémica desencadenada a raíz de ellos los convierte, en su época y para la Historia, en auténticos oráculos, cuyo sentido sus transcripciones, tanto francesa como alemana, inevitablemente en parte desvirtúan. Solo cotejando lo dicho por el maestro, y luego escrito y publicado, con su obra edificada podemos restituir el sentido original de su pensamiento. Esta es la hipótesis sobre la que discurrimos. A sus palabras y obras habrá que sumar la atención que el arquitecto y periodista, que así se confiesa, confiere a los oficios y a los materiales que los sustentan, en la línea de pensamiento de Gottfried Semper, como fundamento del diseño. Es pues a la luz de su concurrencia a la obra de arquitectura, y de ésta al espacio de habitación, y no de otros documentos gráficos y fotográficos, como ha de valorarse lo dicho y escrito por Loos, si nos atenemos a sus propias recomendaciones. Lo dicho se ilumina con lo hecho. Y lo hecho se autoriza por lo habitado.

Karte des Bosporus und der Bithynischen Halbinsel

nach älteren Quellen (v. Moltke, Heinrich Kiepert, Brit. Admiralitätskarten, v. d. Goltz, v. Diest u.a.) und eigenen Aufnahmen im Jahre 1900 gezeichnet von Rudolf Fitzner ; Hof. Stdr. v. J. G. Tiedmann Nachf.

Imperium Turcicum per Europam, Asiam Et Africam se extendens et secundum Beglerbegatus sive Praefecturas accurate distinctum

Covers Libya, Egypt, Sudan, the Arabian Peninsula, Lebanon, Syria, Iraq, Iran, the Balkan Peninsula, Turkey, Greece, Armenia, Azerbaijan and portions of Ethiopia and Russia.

Scaling Social Impact in Europe: Quantitative Analysis of National and Transnational Scaling Strategies of 358 Social Enterprises

A wide variety of different socially motivated organisations perform exceptional feats in alleviating societal ills using creativity and entrepreneurial spirit in their quest to scale their social impact. Their focus is seldom limited to one specific region – instead they strive to benefit the largest possible number of people, thereby often transcending national borders. After all, societal issues usually don’t stop at a countries’ borders – why then should good ideas and impactful concepts aimed at solving these issues? For many socially motivated organisations, the goal of disseminating their social impact remains a solemn wish due to lacking experience and know-how of how to plan and conduct systematic scaling.

Temporal Control of Leaf Complexity by miRNA-Regulated Licensing of Protein Complexes

The tremendous diversity of leaf shapes has caught the attention of naturalists for centuries. In addition to interspecific and intraspecific differences, leaf morphologies may differ in single plants according to age, a phenomenon known as heteroblasty. In Arabidopsis thaliana, the progression from the juvenile to the adult phase is characterized by increased leaf serration. A similar trend is seen in species with more complex leaves, such as the A. thaliana relative Cardamine hirsuta, in which the number of leaflets per leaf increases with age. Although the genetic changes that led to the overall simpler leaf architecture in A. thaliana are increasingly well understood, less is known about the events underlying age-dependent changes within single plants, in either A. thaliana or C. hirsuta. Here, we describe a conserved miRNA transcription factor regulon responsible for an age-dependent increase in leaf complexity. In early leaves, miR319-targeted TCP transcription factors interfere with the function of miR164-dependent and miR164-independent CUC proteins, preventing the formation of serrations in A. thaliana and of leaflets in C. hirsuta. As plants age, accumulation of miR156-regulated SPLs acts as a timing cue that destabilizes TCP-CUC interactions. The destabilization licenses activation of CUC protein complexes and thereby the gradual increase of leaf complexity in the newly formed organs. These findings point to posttranslational interaction between unrelated miRNA-targeted transcription factors as a core feature of these regulatory circuits.

Geschichte und merkwürdigkeiten der Stiftsbibliothek in Zeiz. Als einladung zu einer redeübung in der Stiftsschule zu Zeiz den 16. may 1808.

Mode of access: Internet.

Thesaurus literaturae theologicae academicae; sive, Rcensus dissertationum, programmatum ... cum delectu uberrimo scriptionum academicarum, philologicarum ... quae ab antiquissimis usque ad recentissimia tempora editae in collectione Goethiana Lipsiensi sunt venales.

No more published.

Sämmtliche Werke.

Vol. 1. Erläuterungen und Ergänzungen zum Wesen des Christenthums.- Vol. 2. Philosophische Kritiken und Grundsätze.- Vol. 3. Gedanken über Tod und Unsterblichkeit.- Vol. 4. Geschichte der neuern Philosophie von Bacon von Verulam bis Benedict Spinoza.- Vol. 5. Darstellung, Entwicklung und Kritik der Leibnitz'schen Philosophie.- Vol. 6. Pierre Bayle, ein Beitrag zur Geschichte der Philosophie und Menschheit.- Vol. 7. Das Wesen des Christenthums.- Vol. 8. Vorlesungen über das wesen der Religion.- Vol. 9. Theogonie nachder Quellen des classisischen, hebräischen und christlichen Alterthums.- Vol. 10. Gottheit, Freiheit und Unsterblichkeit vom Standpunkte der Anthropologie.

Beschreibung der groszen Orgel der Marienkirche zu Wismar sowie der groszen Orgel des Domes und der St. Martinikirche zu Halberstadt : ein Beitrag zur Beleuchtung und Würdigung der eigenthümlichen Ansichten und Grundsätze des Herrn Musikdirectors Wilke zu Neu-Ruppin in Bezug auf die Orgelbaukunst von Ferdinand Baake.

Contains history and description of organs built by Friedrich Schulze, David Beck, Josef Wiedemann, and Johann Gottlob Töpfer.

Catalogus bibliothecae numerosae ab incluti nominis viro Hieronymo Guilielmo Ebnero, ab Eschenbach rel. olim conlectae, nunc Norimbergae ... ann. MDCCCXIII[-MDCCCXX] publicae auctionis lege diuendendae. Quem in hunc ordinem redegit, his literarii maximam partem generis notationibus instruxit,

Vol. 1 has added t.-p. (with portrait vignette): Bibliotheca Ebneriana ...

Bibliography on insect nutrition.

Caption title.