Direct Spectrometry: A New Alternative for Measuring the Fluorescence of Composite Resins and Dental Tissues

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

High fluorescence quantum efficiency of CdSe/ZnS quantum dots embedded in GPTS/TEOS-derived organic/silica hybrid colloids

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Functional and Topological Studies with Trp-Containing Analogs of the Peptide StII(1-30) Derived From the N-Terminus of the Pore Forming Toxin Sticholysin II: Contribution to Understand its Orientation in Membrane

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Enhancement of voltammetric determination of quinizarine based on the adsorption at surfactant-adsorbed-layer in disposable electrodes

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Exploring copper nanostructures as highly uniform and reproducible substrates for plasmon-enhanced fluorescence

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Leptomeningeal enhancement in computed tomography of feline brains

Contrast enhancement enables the verification of several pathological conditions that lead to vascular changes and/or breakdown of the blood-brain barrier. Examples of diseases that cause these disorders are: neoplastic diseases, vascular communications, active inflammation and cerebral ischemia. Several contrast enhancements located peripherically to cerebral lobes, in the topography of brain sulci and gyri, were identified on tomographic scan of twelve healthy cats that had their health confirmed through history, general and neurologic physical examination and polymerase chain reaction for feline leukemia (FeLV) and immunodeficiency (FIV) virus. This study aims to describe the tomographic contrast enhancement findings, which showed an identical appearance to the pia mater and arachnoid enhancement, also called leptomeninges. This finding is generally considered related to leptomeningeal diseases such as meningitis and neoplastic disease. However, in dogs, the leptomeningeal enhancement has already been described in healthy animals. This finding has a great importance in the interpretation of tomographic images of these animals since, so far, in the presence of these enhancements, meningeal disorders were suggested. Thus, the verification of other tomographic findings and the combination with other diagnostic methods are of great importance for the diagnosis of leptomeningeal disease.

Imunocastração e ractopamina na dieta de suínos: implicações na qualidade de carne fresca e melhorada por injeção (enhancement)

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Citrus greening disease investigation using micro synchrotron radiation X-ray fluorescence in association with soft independent modelling of class analogy (SIMCA)

This study describes the use of micro synchrotron radiation X-ray fluorescence (µSR-XRF) to investigate citrus greening disease in sweet orange (Citrus sinensis) plants. An experiment using healthy plants as control and plants of the same variety infected with Candidatus Liberibacter asiaticus (CLas) was performed to verify variations of the mineral composition of citrus leaves. A µSR-XRF system using the D09B X-ray fluorescence beam line at the Brazilian Synchrotron Light Source (LNLS, Campinas, São Paulo State) was employed for this purpose. The data were analyzed using a chemometric tool called soft independent modelling of class analogy (SIMCA). The promising results from SIMCA models reinforce the evidence that plants infected by citrus greening (both asymptomatic and symptomatic) undergo alterations in their micro- and macronutrient compositions.

Supported core-shell nanobiosensors for quantitative fluorescence imaging of extracellular pH

Covalent “click” cycloaddition was used to functionalize silica substrates with pH-sensitive nanoparticles, thus producing uniform and highly luminescent analytical devices usable in both commercial fluorimeters and fluorescence microscopes. Quantitative and spatially-resolved extracellular pH measurements were successfully achieved on live cardiac fibroblasts with these novel ion-sensitive surfaces.

Transmission enhancement in chalco-halide glasses for multiband applications

The potential of clear Ga2S3-GeS2-CsCl based sulfide glasses transparent up to 11.5 μm to be used as new optical material for multispectral applications has been investigated. The addition of large amount of chlorine ions – above 40 mol.% of CsCl – into the chalcogenide vitreous network in order to produce colorless glasses results in a drastic increase of their water contamination. We report for the first time, to the best of our knowledge, the purification of cesium chloride CsCl by dynamic distillations under vacuum in order to reduce water and hydroxyl group contamination before complete melting of the glass. Besides, sulfur purification by dynamic and static distillations was also performed in the implemented method. The obtained glasses were then characterized by UV-visible and infrared (FTIR) spectroscopies, by electron probe microanalysis (EPMA), thermal analysis (DSC), and their refractive indices in the visible and near infrared ranges were also measured. A large improvement of the glass transmission spectrum has been achieved with an estimated reduction of about 45 times of the OH and H2O content and 60 times of the SH content. The glass thermal molding ability and chemical durability with and without protective coating have been tested to probe their potential for fabrication of complex optics.

Síntese e estudos de sistemas de bioencapsulação associados a peptídeos antimicrobianos miméticos da toxina natural CcdB

Pós-graduação em Biotecnologia - IQ

In vitro performance of a pen-type laser fluorescence device and bitewing radiographs for approximal caries detection in permanent and primary teeth

To evaluate the performance of a pen‑type laser fluorescence device (DIAGNOdent 2190; LFpen, KaVo, Germany) and bitewing radiographs (BW) for approximal caries detection in permanent and primary teeth. Materials and Methods: A total of 246 anterior approximal surfaces (102 permanent and 144 primary) were selected. Contact points were simulated using sound teeth. Two examiners assessed all approximal surfaces using LFpen and BW. The teeth were histologically assessed for the reference standard. Optimal cut‑off limits were calculated for LFpen for primary and permanent teeth. Sensitivity, specificity, accuracy and area under the receiver operating characteristic curve (Az) were calculated for D1 (enamel and dentin lesions) and D3 (dentin lesions) thresholds. The reproducibility was assessed by intraclass correlation coefficient (ICC) and Cohen's weighted kappa values. Results: For permanent teeth, the LFpen cut‑off were 0– 27 (sound), 28– 33 (enamel caries) and >33 (dentin caries). For primary teeth, the LFpen cut‑off were 0– 7 (sound), 8– 32 (enamelcaries) and >32 (dentin caries). The LFpen presented higher sensitivity values than BW for primary teeth (0.58 vs. 0.32 at D1 and 0.80 vs. 0.47 at D3) and permanent teeth (0.80 vs. 0.57 at D1 and 0.94 vs. 0.51 at D3). Specificity did not show a significant difference between the methods. Rank correlations with histology were 0.59 and 0.83 (LFpen) and 0.36 and 0.70 (BW) for primary and permanent teeth, respectively, considering all lesions. ICC values for LFpen were 0.71 (inter) and 0.86 (intra) for permanent teeth and 0.94 (inter) and 0.90/0.99 for primary teeth. Kappa values for BW were 0.69 (inter) and 0.68/0.90 (intra) for permanent teeth and 0.64 (inter) and 0.89/0.89 for primary teeth. Conclusion: LFpen presented better reproducibility for primary and permanent teeth and higher accuracy in detecting caries lesions at D1 threshold than BW for permanent teeth. LFpen should be used as an adjunct method for approximal caries detection.

The performance of conventional and fluorescence-based methods for occlusal caries detection: an in vivo study with histological validation

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES

Fluorescence Level of Composites Assessed by Computer Processing of Digital Images - ScanWhite

The human dentition is naturally translucent, opalescent and fluorescent. Differences between the level of fluorescence of tooth structure and restorative materials may result in distinct metameric properties and consequently perceptible disparate esthetic behavior, which impairs the esthetic result of the restorations, frustrating both patients and staff. In this study, we evaluated the level of fluorescence of different composites (Durafill in tones A2 (Du), Charisma in tones A2 (Ch), Venus in tone A2 (Ve), Opallis enamel and dentin in tones A2 (OPD and OPE), Point 4 in tones A2 (P4), Z100 in tones A2 ( Z1), Z250 in tones A2 (Z2), Te-Econom in tones A2 (TE), Tetric Ceram in tones A2 (TC), Tetric Ceram N in tones A1, A2, A4 (TN1, TN2, TN4), Four seasons enamel and dentin in tones A2 (and 4SD 4SE), Empress Direct enamel and dentin in tones A2 (EDE and EDD) and Brilliant in tones A2 (Br)). Cylindrical specimens were prepared, coded and photographed in a standardized manner with a Canon EOS digital camera (400 ISO, 2.8 aperture and 1/ 30 speed), in a dark environment under the action of UV light (25 W). The images were analyzed with the software ScanWhite©-DMC/Darwin systems. The results showed statistical differences between the groups (p < 0.05), and between these same groups and the average fluorescence of the dentition of young (18 to 25 years) and adults (40 to 45 years) taken as control. It can be concluded that: Composites Z100, Z250 (3M ESPE) and Point 4 (Kerr) do not match with the fluorescence of human dentition and the fluorescence of the materials was found to be affected by their own tone.

Fluorescence Intensity of Composite Layering Combined with Surface Sealant Submitted to Staining Solutions

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)