Toxicological evaluation of long-term intravenous administration of amitraz in horses

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Tempo de jejum na granja sobre o perfil hormonal e os parâmetros fisiológicos em suínos de abate pesados

O objetivo deste estudo foi avaliar o tempo de jejum na granja e a posição dos animais na carroceria do caminhão durante o transporte ao abatedouro sobre o status hormonal e fisiológico de suínos de abate pesados visando obter melhorias no manejo pré-abate e reduzir perdas na qualidade de carne. Foram utilizadas 64 fêmeas com peso médio de 133+11kg, oriundas de duas granjas de terminação. Os tempos de jejum avaliados foram nove, 12, 15 e 18h, enquanto que as posições consideradas na carroceria foram box (frente, meio e atrás), piso (inferior e superior) e lado (lateral direita e esquerda). Ao abate, foram medidos os níveis de glicose, lactato e CPK no sangue. A concentração de cortisol na saliva (CCS) foi medida nas granjas (24 horas antes e após embarque) e no abatedouro (logo após o descarregamento e antes do abate). A freqüência cardíaca foi monitorada durante todo o manejo pré-abate. Foi observado o efeito da interação entre TJG e o local de avaliação sobre a CCS e a freqüência cardíaca. A CCS e a freqüência cardíaca aumentaram significativamente da granja ao desembarque no abatedouro em relação ao descanso pré-abate no abatedouro foi observada uma redução (P<0,05) nos valores. A CCS variou em função do TJG e o local de avaliação da seguinte maneira: suínos com 18 horas de jejum apresentaram menor (P<0,05) variação na CCS durante o transcorrer das diferentes etapas do manejo pré-abate do que suínos com TJG menores e, entre estes, os animais com TJG de nove horas apresentaram a maior (P<0,05) variação. Antes do abate, os suínos com TJG de nove horas apresentaram o maior valor (P<0,05) de CCS quando comparados aos outros TJG. Conclui-se que o TJG promove mudanças (P<0,05) nos valores do cortisol na saliva e na freqüência cardíaca no manejo pré-abate, mas não afetam (P>0,05) os níveis de glicose, lactato e CPK no abate dos suínos.

Efeito do manejo pré-abate sobre alguns parâmetros fisiológicos em fêmeas suínas pesadas

O objetivo neste trabalho foi avaliar o efeito do período de descanso (3, 5, 7 e 9 horas) dos suínos no frigorífico (PDF) e da localização dos suínos na carroceria do caminhão (PBO), quando transportados, no inverno ou verão, sobre alguns parâmetros fisiológicos avaliados em 64 fêmeas, com peso médio de 130kg para abate, durante o manejo pré-abate. Para a análise estatística, foram considerados, no modelo de análise da variância, os efeitos de bloco, PDF, PBO e da interação (bloco x PDF), entre outros. O PDF influenciou, significativamente, as concentrações de lactato no sangue e cortisol na saliva. Suínos que descansaram 5 e 7 horas apresentaram maior concentração de lactato em relação aos animais que descansaram 3 e 9 horas. No transporte, a freqüência cardíaca foi muito maior em relação aos demais locais avaliados. Concluiu-se que o incremento do PDF não promove mudanças na freqüência cardíaca, nas concentrações de glicose e CPK no sangue e cortisol na saliva, mas interfere na concentração de lactato no sangue dos suínos.

Psicologia e educação no contexto das secretarias municipais: algumas contribuições para novas práticas

Visando a refletir sobre diferentes formas de pensar e fazer dos psicólogos que atuam na Educação Municipal, participamos da Comissão de E-ducação do CRP-06/subsede de Assis e entrevistamos psicólogas aí envolvidas. Verificamos que suas ações - condicionadas ao modelo político-ideológico ado-tado pela gestão municipal e pela formação acadêmica recebida - caracterizam-se por práticas tradicionais e/ou compartilhadas por outros profissionais, que não vêm contribuindo para a reversão do quadro de produção do fracasso escolar. Assim, consideramos urgente a revisão dos aspectos relativos à formação dos psicólogos, às práticas atualmente implementadas e ao engajamento dos órgãos dessa classe para a consolidação de uma psicologia mais comprometida com a cidadania.

Parâmetros bioquímicos de equinos submetidos à simulação de prova de enduro recebendo dietas com adição de óleo de soja

Com o objetivo de verificar o efeito da adição de níveis crescentes de óleo no concentrado sobre a atividade plasmática das enzimas creatina quinase (CK), aspartato aminotransferase (AST) e lactato desidrogenase (LDH) como indicativo de metabolismo energético, foram fornecidas dietas experimentais compostas de cinco níveis de óleo (controle, 6, 12, 18 e 24% do concentrado). Utilizaram-se 20 equinos da raça Árabe, peso médio de 400 kg, submetidos a prova de enduro de 80 km em esteira rolante. O enduro foi dividido em quatro anéis de 20 km, com duração média de 1 hora e dez minutos. A adição de óleo e a distância percorrida tiveram efeitos sobre as variáveis AST, CK e LDH, que apresentaram as respectivas expressões: AST (7,045-0,2292x+0,007991x2+0,008517z- 0,0003282xz), CK (8,06-,07020x+0,05546x2-0,001262x3+0,01204z+0,0006207xz) e LDH (6,624-0,3522x+0,03448x2-0,0008382x3+0,02401z-0,0007489xz) . O óleo é uma importante e bem aproveitada fonte de energia para equinos em exercício, pois sua adição na dieta de animais submetidos a prova de enduro promoveu alteração metabólica que favorece a produção de energia. O metabolismo animal poupou suas reservas energéticas oriundas da glicose, favorecendo a utilização do óleo. A menor atividade plasmática das enzimas AST, CK e LDH com a adição de óleo nas dietas indica direcionamento do metabolismo energético para a β-oxidação. Como apresentam várias isoenzimas, as enzimas estudadas atuam amplamente no metabolismo energético, favorecendo a constante reposição de ATP ao longo do exercício.

Developmental lead exposure: behavioral alterations in the short and long term

Wistar dams were exposed to 500 ppm of Pb, as Ph acetate, or 660 ppm Na acetate in drinking water during pregnancy and lactation. Male pups at 23 (weaned) or 70 days (adult) of age were submitted to behavioral evaluation and Pb determination. The behaviors evaluated were: locomotor activity (open-field test), motor coordination (rotarod test), exploratory behavior (holeboard test), anxiety (elevated plus maze and social interaction tests), and learning and memory (shuttle box). Ph levels were measured in the blood and cerebral regions (hippocampus and striatum) of dams and pups. The results of the present report demonstrated that exposure to Ph during pregnancy and lactation induces in weaned pups hyperactivity, decreased exploratory behavior, and impairment of learning and memory. These alterations were observed at blood Ph levels in the range that may be attained in children chronically exposed to low levels of Pb (21 +/- 3 mug/dl). Regarding adults, the results demonstrated that the regimen of exposure adopted induces anxiety in these animals at nondetectable blood Ph levels. (C) 2001 Elsevier B.V. All rights reserved.

Aerobic training, but not creatine supplementation, alters the gluteus medius muscle

The aim of the present study was to investigate the effect of oral supplementation of creatine on the muscular responses to aerobic training. Twelve purebred Arabian horses were submitted to aerobic training for 90 d, with and without creatine supplementation, and evaluated with respect to BW and BCS and to the area and frequency of the different types of muscle fibers in the gluteus medius. Supplementation consisted of the daily administration of 75 g of creatine monohydrate mixed into the ration for the 90 d of training. Physical conditioning was conducted on a high-performance treadmill, and training intensity was stipulated by calculating the velocity at which blood lactate reaches 4 mmol/L, determined monthly for each animal. The individual intensity of physical force at 80% of aerobic threshold was established. Morphometry of glutens medius muscle fibers was performed on frozen sections processed for histochemical analysis of myosin adenosine triphosphatase and immunohistochemistry of slow-contracting myosin. The results demonstrated that the animals maintained a moderate BCS without alteration of BW during the course of training, providing evidence of equilibrium between food intake and caloric expenditure during the study period. The present study demonstrated that aerobic training for 90 d caused hypertrophy of fiber types I (P = 0.04), IIA (P = 0.04), and IIX (P = 0.01), as well as an increase in the relative area occupied by type I fibers (P = 0.02) at the expense of type IIX fibers (P = 0.03), resulting in modifications of the contractile and metabolic characteristics of the gluteus medius muscle. It was not possible to show any beneficial effect from creatine on the skeletal muscle characteristics examined.

Tracing heme in a living cell: hemoglobin degradation and heme traffic in digest cells of the cattle tick Boophilus microplus

Heme is present in all cells, acting as a cofactor in essential metabolic pathways such as respiration and photosynthesis. Moreover, both heme and its degradation products, CO, iron and biliverdin, have been ascribed important signaling roles. However, limited knowledge is available on the intracellular pathways involved in the flux of heme between different cell compartments. The cattle tick Boophilus microplus ingests 100 times its own mass in blood. The digest cells of the midgut endocytose blood components and huge amounts of heme are released during hemoglobin digestion. Most of this heme is detoxified by accumulation into a specialized organelle, the hemosome.We followed the fate of hemoglobin and albumin in primary cultures of digest cells by incubation with hemoglobin and albumin labeled with rhodamine. Uptake of hemoglobin by digest cells was inhibited by unlabeled globin, suggesting the presence of receptor-mediated endocytosis. After endocytosis, hemoglobin was observed inside large digestive vesicles. Albumin was exclusively associated with a population of small acidic vesicles, and an excess of unlabeled albumin did not inhibit its uptake. The intracellular pathway of the heme moiety of hemoglobin was specifically monitored using Palladium-mesoporphyrin IX (Pd-mP) as a fluorescent heme analog. When pulse and chase experiments were performed using digest cells incubated with Pd-mP bound to globin (Pd-mP-globin), strong yellow fluorescence was found in large digestive vesicles 4 h after the pulse. By 8 h, the emission of Pd-mP was red-shifted and more evident in the cytoplasm, and at 12 h most of the fluorescence was concentrated inside the hemosomes and had turned green. After 48 h, the Pd-mP signal was exclusively found in hemosomes. In methanol, Pd-mP showed maximal emission at 550 nm, exhibiting a red-shift to 665 nm when bound to proteins in vitro.The red emission in the cytosol and at the boundary of hemosomes suggests the presence of heme-binding proteins, probably involved in transport of heme to the hemosome. The existence of an intracellular heme shuttle from the digestive vesicle to the hemosome acting as a detoxification mechanism should be regarded as a major adaptation of ticks to a blood-feeding way of life. To our knowledge, this is the first direct observation of intracellular transport of heme in a living eukaryotic cell. A similar approach, using Pd-mP fluorescence, could be applied to study heme intracellular metabolism in other cell types.

Sensitive Affimer and Antibody Based Impedimetric Label-Free Assays for C-Reactive Protein

C-reactive protein (CRP) is an acute phase protein whose levels are increased in many disorders. Levels greater than 3 mu g/mL serum have hitherto been considered to indicate pathology, but there is increasing interest in assessments between 0.1 and 10 mu g/mL, which have been found to correlate with severity of risk for cardiovascular disease. We report herein the generation of both antibody and Affimer based impedance immunoassays for CRP that are substantially more sensitive than clinically utilized immunonephelometry and immunoturbidity assessments. Significant in this study is not only the use of a constrained peptide to detect a clinically important target but also that derived electrochemical impedance assays can be highly sensitive even with probes whose relatively weak (mu M) affinities are not amenable to target detection by surface plasmon resonance (SPR). Key to this finding is acknowledging that receptive surfaces of comparatively low initial steric bulk and charge transfer resistance are especially primed to be highly responsive to target binding in electroanalytical assays of this type.

Smoking status and tumor necrosis factor-alpha mediated systemic inflammation in COPD patients

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Search for the flavor-changing neutral current decay Bs 0 → μ+ μ- pp̄ Collisions at √s = 1.96 TeV with the DO Detector

We present the results of a search for the flavor-changing neutral current decay Bs 0 → μ+ μ-. using a data set with integrated luminosity of 240 pb-1 of pp̄ collisions at √s = 1.96 TeV collected with the D0 detector in run II of the Fermilab Tevatron collider. We find the upper limit on the branching fraction to be B(Bs 0 → μ+ π-) ≤ 5.0 × 10-7 at the 95% C.L. assuming no contributions from the decay Bd 0 → μ+ μ- in the signal region. This limit is the most stringent upper bound on the branching fraction Bs 0 → μ+ μ- to date. © 2005 The American Physical Society.

Measurement of the pp(-)-> W gamma plus X cross section at root s=1.96 TeV and WW gamma anomalous coupling limits

The WWγ triple gauge boson coupling parameters are studied using pp̄rarr; νγ+X(=e,μ) events at s=1.96 TeV. The data were collected with the D0 detector from an integrated luminosity of 162pb-1 delivered by the Fermilab Tevatron Collider. The cross section times branching fraction for pp̄→W(γ)+X→ νγ+X with ETγ>8 GeV and ΔR γ> 0.7 is 14.8±1.6(stat)±1.0(syst) ±1.0(lum)pb. The one-dimensional 95% confidence level limits on anomalous couplings are -0.88<Δκγ<0.96 and -0. 20<λγ<0.20. © 2005 The American Physical Society.

Measurement of the tt̄ production cross section in pp̄ collisions at √s = 1.96 TeV in dilepton final states

We present a measurement of the top quark pair (tt̄) production cross section in pp̄ collisions at √s=1.96 TeV using events with two charged leptons in the final state. This analysis utilizes an integrated luminosity of 224-243 pb-1 collected with the DØ detector at the Fermilab Tevatron Collider. We observe 13 events in the e+e -, eμ and μ+μ- channels with an expected background of 3.2±0.7 events. For a top quark mass of 175 GeV, we measure a tt̄ production cross section of σtt̄=8. 6-2.7 +3.2(stat)±1.1(syst)±0.6(lumi) pb, consistent with the standard model prediction. © 2005 Elsevier B.V. All rights reserved.

Search for squarks and gluinos in events with jets and missing transverse energy in p(p)over-bar collisions at root s-=1.96 TeV

The results of a search for squarks and gluinos using data from p over(p, ̄) collisions recorded at a center-of-mass energy of 1.96 TeV by the DØ detector at the Fermilab Tevatron Collider are reported. The topologies analyzed consist of acoplanar-jet and multijet events with large missing transverse energy. No evidence for the production of squarks or gluinos was found in a data sample of 310 pb-1. Lower limits of 325 and 241 GeV were derived at the 95% C.L. on the squark and gluino masses, respectively, within the framework of minimal supergravity with tan β = 3, A0 = 0, and μ < 0. © 2006 Elsevier B.V. All rights reserved.

Search for associated Higgs boson production WH→WWW *→l±νl′±ν′+X in pp̄ collisions at s=1.96TeV

We present a search for associated Higgs boson production in the process pp̄→WH→WWW*→l±νl′±ν′ +X in final states containing two like-sign isolated electrons or muons (e±e±, e±μ±, or μ±μ±). The search is based on D0 run II data samples corresponding to integrated luminosities of 360-380pb-1. No excess is observed over the predicted standard model background. We set 95% C.L. upper limits on σ(pp̄→WH) ×Br(H→WW*) between 3.2 and 2.8 pb for Higgs boson masses from 115 to 175 GeV. © 2006 The American Physical Society.