Polimorfismo e classes abstratas

O professor apresenta como utilizar polimorfismo nas linguagens de programação. Explica o que é polimorfismo e demonstra como é utilizado o polimorfismo com as classes já criadas no projeto.

A prática educativa nas classes multisseriadas: impasses e desafios

Este estudo apresenta a sistematização dos resultados da pesquisa sobre a prática educativa numa classe multisseriada. Pretendeu-se, com este trabalho, investigar o tipo de prática que vem sendo desenvolvida no contexto específico de uma sala-de-aula, inserida numa escola da zona rural e de que forma as inovações pedagógicas se refletem na ação do professor junto aos seus alunos. É um estudo de caso etnográfico, de abordagem qualitativa, que permitiu o exame da realidade concreta, mediante análise da documentação, observação das dinâmicas da classe, entrevistas semiestruturadas, inclusive, com gravação em vídeo. A análise do discurso e dos eventos construídos pelos seus principais sujeitos: professor e alunos assumiram grande relevância neste estudo, assim como as relações que ocorreram no âmbito da escola, da família e da comunidade. Por se constituir uma pesquisa de natureza qualitativa, possibilitou certo grau de flexibilidade, oportunizando alterações e ajustes no decorrer do trabalho de campo de forma a alcançar os objetivos pretendidos. As referências teóricas trouxeram contribuições importantes para desvendar os segredos contidos nas práticas educativas vivenciadas nas classes multisseriadas, que de um lado, deparam-se com os impasses perante os elementos que dão sustentação ao processo educacional como um todo e, de outro, enfrentam os desafios de romper com os mecanismos de padronização do sistema seriado e fazer acontecer um novo sentido de ensinar e aprender com as diferenças.

Investigation of urinary volatile organic metabolites as potential cancer biomarkers by solid-phase microextraction in combination with gas chromatography-mass spectrometry

BACKGROUND: Non-invasive diagnostic strategies aimed at identifying biomarkers of cancer are of great interest for early cancer detection. Urine is potentially a rich source of volatile organic metabolites (VOMs) that can be used as potential cancer biomarkers. Our aim was to develop a generally reliable, rapid, sensitive, and robust analytical method for screening large numbers of urine samples, resulting in a broad spectrum of native VOMs, as a tool to evaluate the potential of these metabolites in the early diagnosis of cancer. METHODS: To investigate urinary volatile metabolites as potential cancer biomarkers, urine samples from 33 cancer patients (oncological group: 14 leukaemia, 12 colorectal and 7 lymphoma) and 21 healthy (control group, cancer-free) individuals were qualitatively and quantitatively analysed. Dynamic solid-phase microextraction in headspace mode (dHS-SPME) using a carboxenpolydimethylsiloxane (CAR/PDMS) sorbent in combination with GC-qMS-based metabolomics was applied to isolate and identify the volatile metabolites. This method provides a potential non-invasive method for early cancer diagnosis as a first approach. To fulfil this objective, three important dHS-SPME experimental parameters that influence extraction efficiency (fibre coating, extraction time and temperature of sampling) were optimised using a univariate optimisation design. The highest extraction efficiency was obtained when sampling was performed at 501C for 60min using samples with high ionic strengths (17% sodium chloride, wv 1) and under agitation. RESULTS: A total of 82 volatile metabolites belonging to distinct chemical classes were identified in the control and oncological groups. Benzene derivatives, terpenoids and phenols were the most common classes for the oncological group, whereas ketones and sulphur compounds were the main classes that were isolated from the urine headspace of healthy subjects. The results demonstrate that compound concentrations were dramatically different between cancer patients and healthy volunteers. The positive rates of 16 patients among the 82 identified were found to be statistically different (Po0.05). A significant increase in the peak area of 2-methyl3-phenyl-2-propenal, p-cymene, anisole, 4-methyl-phenol and 1,2-dihydro-1,1,6-trimethyl-naphthalene in cancer patients was observed. On average, statistically significant lower abundances of dimethyl disulphide were found in cancer patients. CONCLUSIONS: Gas chromatographic peak areas were submitted to multivariate analysis (principal component analysis and supervised linear discriminant analysis) to visualise clusters within cases and to detect the volatile metabolites that are able to differentiate cancer patients from healthy individuals. Very good discrimination within cancer groups and between cancer and control groups was achieved.

Cadernos de ensino e aprendizagem de ciências: reflexões sobre uma prática no programa escola ativa em classes multisseriadas

O presente estudo tem como objeto a prática pedagógica na mediação dos Cadernos de Ensino e Aprendizagem da área de Ciências Naturais nas classes multisseriadas, partindo do conceito de inovação pedagógica. Simultaneamente, elabora uma discussão densa acerca do ensinar/aprender ciências; dos Cadernos de Ensino e Aprendizagem; das classes multisseriadas e enfatiza a possibilidade de inter-relação com a inovação pedagógica, ao mesmo tempo em que reflete, explicita e analisa uma experiência pedagógica na qual a educadora compreende o sentido social da sua prática e se institui como organizadora de situações educativas, o que contribui para ratificar o caráter inovador do ensinar/aprender ciências numa classe multisseriada a partir do uso dos cadernos. Para investigar a mediação no uso dos cadernos da área de ciências, optámos pela pesquisa qualitativa, inspirando-nos na etnografia e seus dispositivos de investigação. Durante seis meses, vivenciamos o cotidiano da prática pedagógica da Escola Municipal Leovigildo Ribeiro dos Santos, localizada na zona rural do município de Feira de Santana-Bahia. Para investigar o objeto de pesquisa, utilizámos, dentre outros recursos metodológicos, a entrevista, a observação participante e a filmagem. A pesquisa realizada consubstancia-se numa experiência ímpar ao considerarmos que as mudanças implementadas na prática, de forma intencional e planejada, no sentido de romper com os mecanismos apresentados pelos cadernos apresentam uma nova possibilidade de ensinar/aprender ciências nas classes multisseriadas.

Desenvolvimento de pastas cimentantes utilizando cimentos portland compostos para cimentação de poços petrolíferos

The Compound Portland cements are commonly used in construction, among them stand out the CPII-Z, CPII-F and CPIV. These types of cement have limited application on oil well cementing, having its compositional characteristics focused specifically to construction, as cement for use in oil wells has greater complexity and properties covering the specific needs for each well to be coated. For operations of oil wells cementing are used Portland cements designed specifically for this purpose. The American Petroleum Institute (API) classifies cements into classes designated by letters A to J. In the petroleum industry, often it is used Class G cement, which is cement that meets all requirements needed for cement from classes A to E. According to the scenario described above, this paper aims to present a credible alternative to apply the compound cements in the oil industry due to the large availability of this cement in relation to oil well cements. The cements were micro structurally characterized by XRF, XRD and SEM tests, both in its anhydrous and hydrated state. Later technological tests were conducted to determine the limits set by the NBR 9831. Among the compound cements studied, the CPII-Z showed satisfactory properties for use in primary and secondary operations of oil wells up to 1200 meters cementing

Computational analysis and physico-chemical characterization of an inclusion compound between praziquantel and methyl-beta-cyclodextrin for use as an alternative in the treatment of schistosomiasis

Schistosomiasis is still an endemic disease in many regions, with 250 million people infected with Schistosoma and about 500,000 deaths per year. Praziquantel (PZQ) is the drug of choice for schistosomiasis treatment, however it is classified as Class II in the Biopharmaceutics Classification System, as its low solubility hinders its performance in biological systems. The use of cyclodextrins is a useful tool to increase the solubility and bioavailability of drugs. The aim of this work was to prepare an inclusion compound of PZQ and methyl-beta-cyclodextrin (MeCD), perform its physico-chemical characterization, and explore its in vitro cytotoxicity. SEM showed a change of the morphological characteristics of PZQ:MeCD crystals, and IR data supported this finding, with changes after interaction with MeCD including effects on the C-H of the aromatic ring, observed at 758 cm(-1). Differential scanning calorimetry measurements revealed that complexation occurred in a 1:1 molar ratio, as evidenced by the lack of a PZQ transition temperature after inclusion into the MeCD cavity. In solution, the PZQ UV spectrum profile in the presence of MeCD was comparable to the PZQ spectrum in a hydrophobic solvent. Phase solubility diagrams showed that there was a 5.5-fold increase in PZQ solubility, and were indicative of a type A(L) isotherm, that was used to determine an association constant (K(a)) of 140.8 M(-1). No cytotoxicity of the PZQ:MeCD inclusion compound was observed in tests using 3T3 cells. The results suggest that the association of PZQ with MeCD could be a good alternative for the treatment of schistosomiasis.

A ética econômica das classes trabalhadoras: a gramática social do comportamento econômico da nova pequena burguesia comercial de Natal/RN

Rogram relying on sociological interface between Economic Sociology, Sociology of Moral Theory of Socialization and Social Stratification, this dissertation research makes use of theoretical contributions Luic Boltanski, Charles Taylor, Axel Honneth, Pierre Bourdieu and Bernard Lahire to problematize the generally about the physical and symbolic production and social reproduction of the type of "economic ethics" predominant in the new petite bourgeoisie Brazilian. In other words, the goal is to explain and analyze the objective conditions (economic needs and moral grammar) and intersubjective (modes of socialization and social networks) and update the social genesis and contextual transcontextual beliefs, biases, inclinations and cultural regularities observed the economic behavior of individual profiles for the fractions of the urban petty bourgeoisie and commercial upward Natal / RN. With regard to methodological strategies adopted in data collection will be conducted qualitative interviews (semistructured) and ethnographic notes. In turn, the analytical treatment of the collected empirical content is based on the approach dispositionalist (Pierre Bourdieu, Loïc Wacquant and Bernard Lahire) that emphasizes the study of the past embedded agents and the different contexts of incorporation / activation / inhibition of "provisions" individual cultural

Sobre uma cultura do mal-estar com a política

The current dissertation has as its main object of study the malaise with politics phenomenon. To comprehend it, is carried out in this work a inquiry, in different stages of analysis, based in the empirical data raised by the research Os Processos Sociais de Recepção do Horário Gratuito de Propaganda Eleitoral , made by the Grupo de Estudos Mídia e Poder, of the Universidade Federal do Rio Grande do Norte, during the local elections of 2004 in the city of Natal. Based in the focus group technique, such research raised an ample set of information about the decoding process of the electoral television programs, made by six different groups of citizens from the popular classes. Beginning from the presuppose which such process is influenced by the representations about politics made by those subjects, we use that set of empirical information to inquiry not only the decoding, but that same representations which this process is based. In this way, we analyse, in one side, the globally contrary decoding which subjects made from a conforming code of opposition and, in the other, the structure of feeling which it s based, called structure of feeling of the malaise with politics. Such structure is compound by generating themes which expresses the contraposition about the institutionalized politics and, in the same time, a resignation about politics which fortifies the dominant groups hegemony. We support the thesis which this set of representations about politics is caused by the denial of rights frame which those subjects are immersed

Quantificação das classes de erosão por tipo de uso do solo no município de Franca - SP

Impactos ambientais causados pelas atividades agropecuárias sobre o meio rural podem ser constatados quando são analisadas as paisagens da região. Vários estudos demonstraram que informações da paisagem obtidas a partir de imagens fotográficas são indispensáveis para o diagnóstico do processo erosivo do solo, aspecto indispensável no planejamento conservacionista. Neste trabalho, utilizando-se de técnicas de fotointerpretação, foi realizada a quantificação das classes de erosão acelerada no município de Franca - SP, relacionando-as com os tipos de cobertura vegetal. A coleção de fotografias aéreas empregadas foi produzida pela Base Aerofotogrametria e Projetos S.A., com escala aproximada 1:35.000, em outubro de 1990. Elaboraram-se cartas temáticas do uso/ocupação do solo e dos estados erosivos presentes em cada tipo de cobertura vegetal do solo, estabelecendo-se, em seguida, as relações entre a erosão acelerada e os tipos de comunidades vegetais do município de Franca. Os resultados obtidos mostraram que, nesse município, o solo agrícola encontra-se relativamente conservado, apresentando 74,23% da área com erosão laminar ligeira/moderada. Os diferentes usos/ocupações do solo influenciaram de modos distintos na manifestação do processo erosivo.

Organic acids and/or compound with defined microorganisms to control Salmonella enterica serovar Enteritidis experimental infection in chickens

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)