Shorter CAG repeat in the AR gene is associated with atypical hyperplasia and breast carcinoma

Background: Previous reports into the role of [CAG]n repeat lengths in the androgen receptor (AR) gene indicate that these may play an important part in the development and progression of breast cancer, however, knowledge regarding benign breast lesions is limited. Patients and Methods: PCR-based GeneScan analysis was used to investigate the [CAG]n repeat length at exon 1 of the AR gene in 59 benign breast lesions (27 fibroadenomas, 18 atypical hyperplasias, and 14 hyperplasias without atypia) and 54 ductal breast carcinomas. Seventy-two cancer-free women were used as a control group. In addition, [CAG]n repeats were evaluated for the presence of loss of heterozygosity (LOH) and microsatellite instability (MSI) in a subset of these samples (27 fibroadenomas, 14 hyperplasias without atypia and 22 breast carcinomas). Results: Shorter [CAG]n repeat lengths were strongly correlated with atypical hyperplasias (p=0.0209) and carcinomas (p<0.0001). LOH was found in 1/12 and 4/20 informative cases of hyperplasias without atypia and breast carcinomas, respectively. Three patients with breast carcinoma who had previously presented atypical hyperplasia showed a reduction in the [CAG]n repeat length in their carcinomas. Conclusion: Short [CAG]n repeat length (≤20) polymorphisms are strongly associated with breast carcinomas and atypical hyperplasias. Although non-significant, a subgroup of patients with breast carcinoma and genotype SS showed an association with parameters of worse outcome.

Damage detection in a benchmark structure using AR-ARX models and statistical pattern recognition

Structural health monitoring (SHM) is related to the ability of monitoring the state and deciding the level of damage or deterioration within aerospace, civil and mechanical systems. In this sense, this paper deals with the application of a two-step auto-regressive and auto-regressive with exogenous inputs (AR-ARX) model for linear prediction of damage diagnosis in structural systems. This damage detection algorithm is based on the. monitoring of residual error as damage-sensitive indexes, obtained through vibration response measurements. In complex structures there are. many positions under observation and a large amount of data to be handed, making difficult the visualization of the signals. This paper also investigates data compression by using principal component analysis. In order to establish a threshold value, a fuzzy c-means clustering is taken to quantify the damage-sensitive index in an unsupervised learning mode. Tests are made in a benchmark problem, as proposed by IASC-ASCE with different damage patterns. The diagnosis that was obtained showed high correlation with the actual integrity state of the structure. Copyright © 2007 by ABCM.

Coesão social: inclusão e sentido de pertencer na América Latina e no Caribe. Síntese = Cohesión social: inclusión y sentido de pertenencia en América Latina y el Caribe. Síntesis

Incluye Bibliografía

Indicadores de aplicação e cumprimento da norma ambiental para ar, água e vegetação no Brasil

Incluye Bibliografía

Adubação nitrogenada e critérios de amostragem foliar para a cultura da batata

The present study aimed to determine which leave will be displayed and the time of collection of this leave. The treatments consisted on five doses of nitrogen, four times for collection sampling and three types of leaves, arranged in a randomized block design with three repetitions, totalizing one hundred and forty-four experimental units. The leaves collections took place every fifteen days at thirty, forty-five, sixty and seventy five days after budbreak (DAB), collecting the laminated / compound young leaf (first leave), newly mature (second leave) and the mature leave (third leave) from the set of terminal leaflets. For this was installed an experiment with the culture of potato (cv. Atlantic), in Barretos/SP, the period of march the june of 2010. According to this data collected in this study, the best time for collecting the leaves is at 30 days after the budbreak of the potato cultivation containing nitrogen concentrations in the leaves minus heterogeneous of plants from a fraction to another and with values R2 = 0.98 being higher to the all times of harvests. To the diagnostic leaf, the highest determination coefficient was observed in the newly mature (second leaf) with R2 = 0.98. It can be observed that the highest levels of nitrogen were found on the first leaf (39.01 kg-1). Nevertheless, the values were very heterogeneous and did not fit the curve being the second leave (newly mature) the one that best represents the nutritional status of the plant. Therefore the recommendation for nutritional diagnosis will collect the recently matured leaves (2nd leaf) 30 days after budbreak.

Avaliação dos dados decendiais de precipitação e temperatura máxima e mínima do ar simulados pelo modelo ECMWF para o estado de São Paulo

The weather and climate has a direct influence in agriculture, it affects all stages of farming, since soil preparation to harvest. Meteorological data derived from automatic or conventional weather stations are used to monitor these effects. These meteorological data has problems like difficulty of data access and low density of meteorological stations in Brazil. Meteorological data from atmospheric models, such as ECMWF (European Center for Medium-Range Weather Forecast) can be an alternative. Thus, the aim of this study was to compare 10-day period precipitation, maximum and minimum air temperature data from the ECMWF model with interpolated maps from 33 weather stations in Sao Paulo state between 2005 and 2010 and generate statistical maps pixel by pixel. Statistical index showed spatially satisfactory (most of the results with R 2 > 0.60, d > 0.7, RMSE < 5°C and < 50 mm; Es < 5°C and < 24 mm) in period and ECMWF model can be recommended for use in the Sao Paulo state.

Structural Health Monitoring based on AR models and PZT sensors

This paper presents a new approach for damage detection in Structural Health Monitoring (SHM) systems, which is based on the Electromechanical Impedance (EMI) principle and Autoregressive (AR) models. Typical applications of EMI in SHM are based on computing the Frequency Response Function (FRF). In this work the procedure is based on the EMI principle but the results are determined through the coefficients of AR models, which are computed from the time response of PZT transducers bonded to the monitored structure, and acting as actuator and sensors at the same time. The procedure is based on exciting the PZT transducers using a wide band chirp signal and getting its time response. The AR models are obtained in both healthy and damaged conditions and used to compute statistics indexes. Practical tests were carried out in an aluminum plate and the results have demonstrated the effectiveness of the proposed method. © 2012 IEEE.

NF-kB overexpression and decreased immunoexpression of AR in the muscular layer is related to structural damages and apoptosis in cimetidine-treated rat vas deferens

Background: Cimetidine, histamine H2 receptors antagonist, has caused adverse effects on the male hormones and reproductive tract due to its antiandrogenic effect. In the testes, peritubular myoid cells and muscle vascular cells death has been associated to seminiferous tubules and testicular microvascularization damages, respectively. Either androgen or histamine H2 receptors have been detected in the mucosa and smooth muscular layer of vas deferens. Thus, the effect of cimetidine on this androgen and histamine-dependent muscular duct was morphologically evaluated.Methods: The animals from cimetidine group (CMTG; n=5) received intraperitoneal injections of 100 mg/kg b.w. of cimetidine for 50 days; the control group (CG) received saline solution. The distal portions of vas deferens were fixed in formaldehyde and embedded in paraffin. Massońs trichrome-stained sections were subjected to morphological and the following morphometrical analyzes: epithelial perimeter and area of the smooth muscular layer. TUNEL (Terminal deoxynucleotidyl-transferase mediated dUTP Nick End Labeling) method, NF-kB (nuclear factor kappa B) and AR (androgen receptors) immunohistochemical detection were also carried out. The birefringent collagen of the muscular layer was quantified in picrosirius red-stained sections under polarized light. The muscular layer was also evaluated under Transmission Electron Microscopy (TEM).Results: In CMTG, the mucosa of vas deferens was intensely folded; the epithelial cells showed numerous pyknotic nuclei and the epithelial perimeter and the area of the muscular layer decreased significantly. Numerous TUNEL-labeled nuclei were found either in the epithelial cells, mainly basal cells, or in the smooth muscle cells which also showed typical features of apoptosis under TEM. While an enhanced NF-kB immunoexpression was found in the cytoplasm of muscle cells, a weak AR immunolabeling was detected in these cells. In CMTG, no significant difference was observed in the birefringent collagen content of the muscular layer in comparison to CG.Conclusions: Cimetidine induces significant damages in the epithelium; a possible antiandrogenic effect on the basal cells turnover should be considered. The cimetidine-induced muscle cells apoptosis confirms the susceptibility of these cells to this drug. The parallelism between enhanced cytoplasmic NF-kB immunolabeling in the damaged muscular tissue and muscle cell apoptosis suggests that this drug may avoid the translocation of NF-kB to the nucleus and interfere in the control of NF-kB-mediated smooth muscle cell apoptosis. The decreased immunoexpression of ARs verified in the damaged muscular tissue reinforces this possibility. © 2013 Koshimizu et al.; licensee BioMed Central Ltd.

Adubação mineral, esterco de curral e lodo de esgoto no desenvolvimento inicial do cafeeiro

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Assistência de ar e volumes de pulverização na deposição de gotas e controle do arroz vermelho (Oryza sativa L.)

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Efeitos do preenchimento do balonete do tubo traqueal com ar ou lidocaína a 2% alcalinizada na pressão do balonete e na morbidade laringotraqueal durante anestesia com óxido nitroso

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

AR | RA: a arte na realidade aumentada

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

A revista Artéria: uma amostragem das poéticas intersemióticas dos anos 70 aos 90

Pós-graduação em Artes - IA

Amostragem de folhas em cafeeiro para fins de diagnose do estado nutricional

Pós-graduação em Agronomia (Ciência do Solo) - FCAV

Retratos sociológicos de estudantes de Pedagogia: o caso da FCL/Ar

Pós-graduação em Educação Escolar - FCLAR