Caracterização fisiológica e bioquímica da dominância apical em bananeira (Musa acuminata colla)

Pós-graduação em Agronomia (Horticultura) - FCA

Identidade e hibidrismo em Dalcídio Jurandir: a formação identitária de Alfredo, em Três casas e um rio

Este trabalho realiza uma leitura da obra Três Casas e um rio de Dalcídio Jurandir, seguindo alguns percursos para a formação da identidade de Alfredo. Preocupa-nos, primeiramente, verificar como a identidade de Alfredo se articula diante das diferenças culturais e dicotomias como popular/erudito; oral/escrito. Popular e oral, ligados a valores da mãe negra, D.Amélia, e erudito e escrito, relacionado ao pai, Major Alberto. Em seguida, apresenta os traços fronteiriços auxiliadores da elaboração da identidade de Alfredo, Finalmente, verifica-se como as narrativas presentes na obra estão incorporadas no texto dalcidiano, e sua importância para a resolução de alguns conflitos vivenciados pelo protagonista.E para que se possa visualizar a abordagens teóricas aqui trabalhadas, contribuem com este estudo autores como Stuart Hall, D. Shüller, Zilá Bernd e, ainda, serão citados Walter Benjamin, Anthony Giddens, entre outros.

A reeleição de vereadores evangélicos em Belém de 1992 a 2008: conexão, regra do jogo e comunicação com as bases

Em 2004 foram eleitos os vereadores que compunham a Câmara Municipal de Belém, tendo sido ocupadas às vagas deixadas por aqueles que não se reelegeram. Esse quadro da reeleição possibilitou a afirmação e fortalecimento de partidos políticos que, por vários mandatos, permanecem com representação política e assento no Parlamento Municipal. É o caso do Partido dos Trabalhadores (PT), do Partido do Movimento Democrático Brasileiro (PMDB), do Partido da Social Democracia Brasileira (PSDB), do Partido Trabalhista Brasileiro (PTB) entre outros.Esse grupo político tem estado representado no Parlamento municipal por um longo período ininterrupto. O campeão de renovação de mandato é o Vereador Pastor Paulo Queiroz da IEQ (Igreja do Evangelho Quadrangular) que está no seu quarto mandato. Em seguida, vem o Vereador Pastor Raul Batista da IURD (Igreja Universal do Reino de Deus) que está no seu terceiro mandato e o Vereador Iran Moraes da AD (Assembleia de Deus) que está no seu terceiro mandato. O ponto de partida da pesquisa, parte dos pressupostos sugeridos por Pereira e Rennó (2001) e sustentado por Downs (1958) de que, o objetivo central de um político é a busca de reeleição.Anthony Downs considera que o individuo dentro da sua racionalidade, tem a capacidade de interpretar as suas escolhas dentro de uma lógica de alternativas possíveis. Ele não se deixa ser seduzido pelos movimentos dos candidatos, mas, faz a sua escolha dentro das alternativas que se apresenta, mas mantém as escolhas racionais. Percebe-se que ao longo dos anos, os parlamentares evangélicos têm demonstrado maior força em algumas denominações em se manter no parlamento para vários mandatos. É o caso dos parlamentares da Igreja do Evangelho Quadrangular, Adventista do Sétimo Dia, Universal do reino de Deus, Assembleia de Deus. O processo de renovação contínua de mandato é baseado no apoio das lideranças e nos serviços prestados aos seguimentos internos das Igrejas.

Estudos latino-americanos em Oxford: notas sobre a universidade do Oxford colhidas durante a realizaçãode um seminário sobre o Brasil atual

Estas notas têm como intenção mais imediata divulgar as possibilidades de estudos no nível de pós-graduação e pós-doutorado em áreas ligadas às ciências sociais, à literatura e às humanidades de modo geral, na Universidade de Oxford, através do St. Antony's College, que é a sua Instituição especializada nesse tipo de estudos em relação a países de todo o mundo.

Linguagem narrativa e fluência na síndrome de down: uma revisão

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Intervenção em habilidades sociais com uma criança com síndrome de Down

Studies show that children with Down’s syndrome have deficits in social skills, which can interfere in their social interactions and also in their academic performance. The aim of this study was to analyze the clinical significance and reliable change in repertory of social skills of a child with Down’s syndrome, from interventions in the clinic, at home and at school. A seven year old girl with Down’s Syndrome who had frequented the first year of regular school, her parents and her teacher participated in this study. They were attending in a Center of Applied Psychology, of a public university in São Paulo State. Before and after the intervention parents and teacher answered the Rating System Social Skills (SSRS). The intervention with the child had been conducted for six months, through playful activities in weekly meetings. It was discussed various topics related to social skills with parents and teacher in biweekly and monthly meetings. The results were analyzed using JT method. With parents, comparing the two assessments conducted, the data pointed to positive change reliable for four of the six skills assessed. Of the five skills assessed by the teacher, one of them was not in the clinical level since the first application. The other two went from clinical to non-clinical level, featuring reliable positive change. The study showed the importance of pre and post measures in case study, enabling the assessment of the effects of the intervention performed on the various factors of social skills. Further studies with larger populations may ratify the data obtained.

RB32-266 Cooling Milk on Nebraska Farms

The desire of Nebraska people to continue the improvement of living conditions and to secure more healthful foods has been responsible for many changes in methods of caring for milk. One of the important factors in keeping milk sweet and of good quality is the process of cooling and keeping it cool until used. Three of these processes are as follows: placing containers of warm milk in any quantity of still water or still air at temperatures ranging from freezing to within a few degrees of the temperature of the milk, placing the containers in such positions that air or water are circulated around them, and causing the milk to flow in such manner that a thin film comes in contact with a surface which is cooled by air or liquids varying in temperature from 10 degrees Fahrenheit to a few degrees below that of the milk. After some of the heat has been removed the milk is stored under conditions very similar to those found in cooling processes. This 1932 research bulletin discusses why milk is cooled, why milk sours, how bacteria grows, and the many ways that milk can be cooled.

RB32-270 A Seven Year Study of a Milk Supply

The amount of butter produced by the grain-belt states is evidence that a great many cows are milked by the midwestern farmer. Most of this milk is separated on the farm, the cream is sold, and the skimmilk is fed to hogs and other livestock. As the market for fluid milk has developed, many farmers near the cities have turned to the sale of milk, because it affords a better return for the butterfat sold. Much of the milk produced for sale as fluid milk is produced under practically the same conditions as milk which is produced primarily for the same of cream. The Department of Dairy Husbandry of the University of Nebraska, in conducting its instructional and investigational work, comes in contact with the milk producer. An effort has been made, therefore, to study the relation of milk quality to farm conditions as found among the milk producers or patrons who have delivered milk to the department. The study was carried on in an effort to find possible ways of bettering the conditions without upsetting the economic balance existing between the production of cream and fluid milk.

Nachweis von DNA-Doppelstrangbrüchen über gamma-H2AX: Immunhistochemie nach Einwirkung gentoxischer Agenzien auf Säugerzellen

Wie im Rahmen dieser Arbeit bestätigt werden konnte, eignet sich die Quantifizierung von γ-H2AX-Foci mittels Immunfluoreszenz zur Quantifizierung von DNA-Doppelstrangbrüchen, welche durch ionisierende Strahlung erzeugt werden. Dabei erzeugt ein Gy Strahlung der verwendeten 60Co-Quelle 33,8 ± 2,1 DNA-Doppelstrangbrüche. Durch UV-Strahlung sowie alkylierende Substanzen wie MMS und MNNG werden in CHO-Zellen γ-H2AX-Foci induziert. Die Anzahl der induzierten γ-H2AX-Foci ist Dosis- und replikationsabhängig. Die im Rahmen dieser Arbeit erhobenen Daten sprechen für eine Phosphorylierung von H2AX an Läsionen, welche die DNA-Replikation beeinträchtigen und insbesondere aktive Replikationsgabeln blockieren. Diese Läsionen können zu DNA-Doppelstrangbrüchen an blockierten Replikationsgabeln führen H2AX wird in der unmittelbaren Umgebung von DNA-Doppelstrangbrüchen zu γ-H2AX phosphoryliert und eignet sich damit zur Quantifizierung dieser Läsionen. Ob γ-H2AX ausschließlich an DNA-Doppelstrangbrüchen phosphoryliert wird, oder auch an anderen Läsionen ist in der Literatur umstritten. Die bis dato publizierte Literatur geht mehrheitlich davon aus, dass γ-H2AX einen ausschließlichen Marker von DNA-Doppelstrangbrüchen darstellt (Burma et al., 2001; Fernandez-Capetillo et al., 2004; Foster und Downs, 2005; Furuta et al., 2003; Halicka et al., 2005; Huang et al., 2005; Paull et al., 2000; Redon et al., 2002; Stucki und Jackson, 2006; Takahashi und Ohnishi, 2005; Ward und Chen, 2001). Neuere Arbeiten postulieren jedoch, dass H2AX auch durch andere, bisher nicht genau klassifizierte, Störungen der Chromatinstruktur phosphoryliert wird (Marti et al., 2006; Stojic et al., 2004). Die im Rahmen dieser Arbeit dargestellten Ergebnisse mit UV-Strahlung und den Alkylantien MMS und MNNG lassen sich gut durch die teils direkte, größtenteils jedoch replikationsabhängige Bildung von DNA-Doppelstrangbrüchen an blockierten Replikationsgabeln erklären. Ausschließen lässt sich die Hypothese, dass die beobachteten γ-H2AX-Foci auch aufgrund anderer Läsionen entstehen, auf Grundlage der erhaltenen Daten nicht. Die Quantifizierung von γ-H2AX eignet sich zur Darstellung von durch ionisierende Strahlung, UV-Strahlung sowie Alkylantien erzeugten Effekten. Eine abschließende Klärung, ob durch die hier angewandte Methode selektiv DNA-Doppelstrangbrüche detektiert werden, steht aber weiterhin aus.

Analysis of the influence of epitope flanking regions on MHC class I restricted antigen presentation

Peptides presented by MHC class I molecules for CTL recognition are derived mainly from cytosolic proteins. For antigen presentation on the cell surface, epitopes require correct processing by cytosolic and ER proteases, efficient TAP transport and MHC class I binding affinity. The efficiency of epitope generation depends not only on the epitope itself, but also on its flanking regions. In this project, the influence of the C-terminal region of the model epitope SIINFEKL (S8L) from chicken ovalbumin (aa 257-264) on antigen processing has been investigated. S8L is a well characterized epitope presented on the murine MHC class I molecule, H-2Kb. The Flp-In 293Kb cell line was transfected with different constructs each enabling the expression of the S8L sequence with different defined C-terminal flanking regions. The constructs differed at the two first C-terminal positions after the S8L epitope, so called P1’ and P2’. At these sites, all 20 amino acids were exchanged consecutively and tested for their influence on H-2Kb/S8L presentation on the cell surface of the Flp-In 293Kb cells. The detection of this complex was performed by immunostaining and flow cytometry. The prevailing assumption is that proteasomal cleavages are exclusively responsible for the generation of the final C-termini of CTL epitopes. Nevertheless, recent publications showed that TPPII (tripeptidyl peptidase II) is required for the generation of the correct C-terminus of the HLA-A3-restricted HIV epitope Nef(73-82). With this background, the dependence of the S8L generation on proteasomal cleavage of the designed constructs was characterized using proteasomal inhibitors. The results obtained indicate that it is crucial for proteasomal cleavage, which amino acid is flanking the C-terminus of an epitope. Furthermore, partially proteasome independent S8L generation from specific S8L-precursor peptides was observed. Hence, the possibility of other existing endo- or carboxy-peptidases in the cytosol that could be involved in the correct trimming of the C-terminus of antigenic peptides for MHC class I presentation was investigated, performing specific knockdowns and using inhibitors against the target peptidases. In parallel, a purification strategy to identify the novel peptidase was established. The purified peaks showing an endopeptidase activity were further analyzed by mass spectrometry and some potential peptidases (like e.g. Lon) were identified, which have to be further characterized.

Progettazione e prototipazione di un sistema di Social Business Intelligence con Hadoop Impala

Il presente elaborato ha come oggetto l’analisi delle prestazioni e il porting di un sistema di SBI sulla distribuzione Hadoop di Cloudera. Nello specifico è stato fatto un porting dei dati del progetto WebPolEU. Successivamente si sono confrontate le prestazioni del query engine Impala con quelle di ElasticSearch che, diversamente da Oracle, sfrutta la stessa componente hardware (cluster).

Randomized clinical trial comparing percutaneous closure of patent foramen ovale (PFO) using the Amplatzer PFO Occluder with medical treatment in patients with cryptogenic embolism (PC-Trial): rationale and design

Background Several studies have shown an association of cryptogenic stroke and embolism with patent foramen ovale (PFO), but the question how to prevent further events in such patients is unresolved. Options include antithrombotic treatment with warfarin or antiplatelet agents or surgical or endovascular closure of the PFO. The PC-Trial was set up to compare endovascular closure and best medical treatment for prevention of recurrent events. Methods The PC-Trial is a randomized clinical trial comparing the efficacy of percutaneous closure of the PFO using the Amplatzer PFO occluder with best medical treatment in patients with cryptogenic embolism, i.e. mostly cryptogenic stroke. Warfarin for 6 months followed by antiplatelet agents is recommended as medical treatment. Randomization is stratified according to patients age (<45 versus ≥45 years), presence of atrial septal aneurysm (ASA yes or no) and number of embolic events before randomization (one versus more than one event). Primary endpoints are death, nonfatal stroke and peripheral embolism. Discussion patients were randomized in 29 centers of Europe, Canada, and Australia. Randomization started February 2000. Enrollment of 414 patients was completed in February 2009. All patients will be followed-up longitudinally. Follow-up is maintained until the last enrolled patient is beyond 2.5 years of follow-up (expected in 2011).

Antibodies recognising sulfated carbohydrates are prevalent in systemic sclerosis and associated with pulmonary vascular disease

Glycosylation represents an important modification that regulates biological processes in tissues relevant for disease pathogenesis in systemic sclerosis (SSc), including the endothelium and extracellular matrix. Whether patients with SSc develop antibodies to carbohydrates is not known.

The Charity and the Poverty

At the end of the middle Ages, financial savings institutions developed, largely in response to the increasingly usurious money-lending practices of the Jews and to the adaptation of church authorities to the by then well developed commercial and financial operations. It was the Franciscan Order that took the initiative to develop such institutions, first in Italy and later in other western European Mediterranean towns. These were the so-called Monte di Pieta, which lent money at low rates of interest taking objects in pawn as security. However, as they had to operate with circulating capital and on the principles of savings banks, they may be considered to be the predecessors of modern banks. Although charity was declared in the very names of these institutions, this was no longer in the sense of the medieval mercy towards the virtuous poor, but more a support for impoverished members of higher social strata, as loan applicants had to place valuable movable property in pawn, meaning that they first had to possess such property. In spite of this, the institutions had the character of the primeval accumulation of capital, although not so much for individuals as for a community or, in the latter's name, for a commune, i.e. the local authority, which at least in the cases of Koper and Piran was also the founder. However, the stagnation of trade with the hinterland and the decline in the economic power of the Venetian Republic, particularly in the late 17th and early 18th centuries, pushed pawnshops into a more miserable existence, with ups and downs linked with the irregularities of profit-making on behalf of the institution, particularly by its clerks.

Keratinocyte transcriptional regulation of the human c-Myc promoter occurs via a novel Lef/Tcf binding element distinct from neoplastic cells

The proto-oncogene c-Myc is involved in early neoplastic transformations. Two consensus Lef/Tcf binding elements (TBE) were found to be prerequisite for transcriptional transactivation by the armadillo proteins beta-catenin and plakoglobin (PG) together with Tcf4 in human neoplastic cells. In epidermal keratinocytes, c-Myc was reported to be repressed by Lef-1 and PG. Using reporter gene assays, here we demonstrate that deletion of the two consensus TBE fails to abrogate transcriptional regulation by Lef-1/PG in wildtype and beta-catenin-/- keratinocytes, while it reduces transcription in pre-neoplastic PG-/- keratinocytes. We identified a TBE sequence variant downstream of the major transcriptional initiation site that binds Lef-1 in vitro and in vivo, and its mutation compromised transcriptional regulation by Lef-1/PG. Collectively, this study demonstrates that the two consensus TBE's reported in neoplastic cells are dispensable for c-Myc regulation in normal keratinocytes, which instead use a novel TBE sequence variant. This unprecedented finding may have important implications for armadillo target genes involved in carcinogenesis.