Impact of a 2-year intervention program on cardiometabolic profile according to the number of goals achieved

We investigated the impact of lifestyle goal achievement on cardiovascular risk factors after a 2-year behavioral intervention program applied to 394 adults (113 with diabetes, mean age 60.2 ± 11.4 years, 56% women) and targeting four goals: ≥5% weight loss; ≥150 min/week physical activities; <10% saturated fat intake/day; ≥400 g fruit and vegetable intake/day. Baseline characteristics and changes in variables after intervention among the four categories of number of goals achieved (none, 1, 2, and ≥3) were compared by independent ANOVA or the Kruskal-Wallis test. Individuals without diabetes achieving a higher number of goals were more likely to be older (3 or 4 goals: 61.8 ± 12.6 years vs none: 53.3 ± 10.3 years, P < 0.05) and to have a lower mean BMI (3 or 4 goals: 21.7 ± 2.6 kg/m² vs none: 29.0 ± 4.8 kg/m², P < 0.05), diastolic blood pressure (3 or 4 goals: 77.3 ± 2.1 mmHg vs none: 85.4 ± 9.6 mmHg, P < 0.05), triglyceride (3 or 4 goals: 116.1 ± 95.1 mg/dL vs none: 144.8 ± 65.5 mg/dL, P < 0.05) and insulin levels (3 or 4 goals: 3.6 ± 2.4 _6;U/L vs none: 5.7 ± 4.0 _6;U/L, P < 0.05) than those achieving fewer goals. The absolute changes in cardiovascular risk factors tended to be more pronounced with increasing number of goals achieved in individuals without diabetes. The intervention had a beneficial impact on the cardiometabolic profile of individuals with normal or altered glucose metabolism. The number of goals achieved in this lifestyle intervention was associated with the magnitude of improvement of cardiovascular risk factors in individuals without diabetes. Participants with a better cardiometabolic profile seemed to be more likely to have a healthy lifestyle.

Relationship of macrophage migration inhibitory factor levels in PBMCs, lesional skin and serum with disease severity and activity in vitiligo vulgaris

Melanocyte loss in vitiligo vulgaris is believed to be an autoimmune process. Macrophage migration inhibitory factor (MIF) is involved in many autoimmune skin diseases. We determined the possible role of MIF in the pathogenesis of vitiligo vulgaris, and describe the relationship between MIF expressions and disease severity and activity. Serum MIF concentrations and mRNA levels in PBMCs were measured in 44 vitiligo vulgaris patients and 32 normal controls, using ELISA and real-time RT-PCR. Skin biopsies from 15 patients and 6 controls were analyzed by real-time RT-PCR. Values are reported as median (25th-75th percentile). Serum MIF concentrations were significantly increased in patients [35.81 (10.98-43.66) ng/mL] compared to controls [7.69 (6.01-9.03) ng/mL]. MIF mRNA levels were significantly higher in PBMCs from patients [7.17 (3.59-8.87)] than controls [1.67 (1.23-2.42)]. There was also a significant difference in MIF mRNA levels in PBMCs between progressive and stable patients [7.86 (5.85-9.13)vs 4.33 (2.23-8.39)] and in serum MIF concentrations [40.47 (27.71-46.79) vs 26.80 (10.55-36.07) ng/mL]. In addition, the vitiligo area severity index scores of patients correlated positively with changes of both serum MIF concentrations (r = 0.488) and MIF mRNA levels in PBMCs (r = 0.426). MIF mRNA levels were significantly higher in lesional than in normal skin [2.43 (2.13-7.59)vs 1.18 (0.94-1.83)] and in patients in the progressive stage than in the stable stage [7.52 (2.43-8.84)vs 2.13 (1.98-2.64)]. These correlations suggest that MIF participates in the pathogenesis of vitiligo vulgaris and may be useful as an index of disease severity and activity.

A phthalide derivative isolated from endophytic fungiPestalotiopsis photiniae induces G1 cell cycle arrest and apoptosis in human HeLa cells

MP [4-(3′,3′-dimethylallyloxy)-5-methyl-6-methoxyphthalide] was obtained from liquid culture of Pestalotiopsis photiniaeisolated from the Chinese Podocarpaceae plant Podocarpus macrophyllus. MP significantly inhibited the proliferation of HeLa tumor cell lines. After treatment with MP, characteristic apoptotic features such as DNA fragmentation and chromatin condensation were observed in DAPI-stained HeLa cells. Flow cytometry showed that MP induced G1 cell cycle arrest and apoptosis in a dose-dependent manner. Western blotting and real-time reverse transcription-polymerase chain reaction were used to investigate protein and mRNA expression. MP caused significant cell cycle arrest by upregulating the cyclin-dependent kinase inhibitor p27KIP1 protein and p21CIP1 mRNA levels in HeLa cells. The expression of p73 protein was increased after treatment with various MP concentrations. mRNA expression of the cell cycle-related genes, p21CIP1, p16INK4a and Gadd45α, was significantly upregulated and mRNA levels demonstrated significantly increased translation ofp73, JunB, FKHR, andBim. The results indicate that MP may be a potential treatment for cervical cancer.

Changes in the physicochemical and microbiological properties of frozen araça pulp during storage

Araça belongs to the Myrtaceae family and is popularly known as araçá-comum, araçá-azedo, or araçá-do-campo. Frozen fruit pulp is of great importance for the food industry, which can produce it at the time of harvest, store it, and use it according to the demand of the consumer market and/or as an ingredient in the formulation of products such as yogurt, candies, and ice creams among others. The aim of this study was to evaluate the physical, chemical, and microbiological stability of frozen araça pulp during 12 months of frozen storage. It was observed that the levels of moisture (90.55-88.75%), ash (0.34-0.26%) total soluble sugars (7.11-6.62%), sucrose (3.55-1.39%), soluble pectin (0.24-0.23%), total pectin (0.5-0.46%), pH (3.82-2.31%), organic acids (698.12-122.25 µg.g-1 citric acid), and phenolic compounds (6.22-0.00 mg GAE.100 g-1) decreased during storage, whereas the levels of protein (0.61-0.83%), lipids (0.14-0.38%), total carbohydrates (8.36-9.78%), calorific value (37.14-45.86 kcal.100 g-1), reducing sugars (3.51-5.21%), soluble solids (5.17-6.0%), total antioxidant capacity (6.89-35.13%), and color parameters (L*49.75-50.67; a*0.79-1.82 and b*22.5-25.19) increased over the one-year storage period. According to the chemical and microbiological parameters assessed, the product can be stored for 12 months without loss of quality with addition of citric acid as a preservative.

Identification and characterisation of phenolic compounds extracted from Moroccan olive mill wastewater

Olive mill wastewater, hereafter noted as OMWW was tested for its composition in phenolic compounds according to geographical areas of olive tree, i.e. the plain and the mountainous areas of Tadla-Azilal region (central Morocco). Biophenols extraction with ethyl acetate was efficient and the phenolic extract from the mountainous areas had the highest concentration of total phenols' content. Fourier-Transform-Middle Infrared (FT-MIR) spectroscopy of the extracts revealed vibration bands corresponding to acid, alcohol and ketone functions. Additionally, HPLC-ESI-MS analyses showed that phenolic alcohols, phenolic acids, flavonoids, secoiridoids and derivatives and lignans represent the most abundant phenolic compounds. Nüzhenide, naringenin and long chain polymeric substances were also detected. Mountainous areas also presented the most effective DPPH scavenging potential compared to plain areas; IC50 values were 11.7 ± 5.6 µg/ml and 30.7 ± 4.4 µg/ml, respectively. OMWW was confirmed as a rich source of natural phenolic antioxidant agents.

Efeitos a curto prazo da dieta com proteína da soja em pacientes com glomerulopatias proteinúricas

INTRODUÇÃO: Há indícios de que a proteína da soja poderia contribuir para reduzir a velocidade de progressão da doença renal, diminuindo colesterol sérico e proteinúria em pacientes com nefropatias. Este estudo foi desenvolvido para avaliar o efeito da die>ta com proteína da soja sobre proteinúria e dislipidemia, em pacientes com glomerulopatias proteinúricas. PACIENTES E MÉTODOS: Os pacientes foram divididos em três grupos: o Grupo Controle (n = 9) recebeu dieta com 0,8 g/kg/dia de proteína animal; o Grupo de Estudo 1 (n = 9) recebeu dieta com 0,8 g/kg/dia de proteína da soja e o Grupo 2 (n = 9), dieta com 0,8 g/kg/dia de proteína da soja mais fibras. O período de estudo foi de oito semanas. Durante o período basal e no final do estudo, os pacientes foram submetidos à avaliação laboratorial e antropométrica. RESULTADOS: Não foram observadas diferenças estatisticamente significantes entre os períodos pré e pós-intervenção em nenhum dos grupos estudados, nos parâmetros antropométricos ou na composição corporal entre os três grupos, nem nos níveis de proteinúria (Controle: 0.7 ± 0.6 versus 0.8 ± 0.6; Grupo 1: 2.0 ± 1.7 versus 1.9 ± 1.8; Grupo 2: 2.0 ± 1.4 versus 2.1 ± 2.0). No entanto, observou-se discreta diminuição nos níveis triglicérides (244.8+-275.9 versus 200.5+-34.0), colesterol total (234.0+-59.4 versus 181.2+-110.3) e LDL (136.0+-59.1 versus 104.1+-39.4) no Grupo 1, embora sem atingir significância estatística. CONCLUSÃO: Não foram detectados efeitos benéficos com a substituição da proteína animal pela proteína da soja em relação aos objetivos de reduzir proteinúria e hiperlipidemia; porém, constatou-se que a dieta de proteína da soja não causou alterações deletérias na composição corporal, mantendo um estado nutricional adequado.

Efeito do tratamento químico sobre a conservação de sementes de milho durante o armazenamento

O objetivo do trabalho foi estudar o efeito de combinações de inseticidas e de fungicidas sobre a conservação de sementes de milho durante o armazenamento. Os tratamentos aplicados e respectivas doses, para 1000kg de sementes, foram os seguintes: 1) testemunha; 2) anilina (936mL); 3) anilina (936mL) + Thiabendazole (120g) + Dicarboximida (187,5g) + Pirimifos- methyl (6,25mL) + Deltamethrin (0,75mL); 4) anilina (936mL) + Thiabendazole (240g) + Dicarboximida (375,0g) + Pirimifos- methyl (12,50mL) + Deltamethrin (1,50mL); 5) anilina (936mL) + Thiabendazole (480g) + Dicarboximida (750,0g)+ Pirimifos- methyl (25,00mL) + Deltamethrin (3,00mL); 6) anilina (936mL) + Thiabendazole (960g) + Dicarboximida (1500,0g) + Pirimifos- methyl (50,00mL) + Deltamethrin (6,00mL). As sementes foram analisadas quanto ao teor de água, germinação e vigor (testes de envelhecimento acelerado e de frio com solo) aos 0, 6, 9 e 12 meses de armazenamento. Os dados obtidos permitiram concluir que os tratamentos químicos aplicados tendem, com o aumento das dosagens, a gerar efeitos latentes, desfavoráveis ao desempenho das sementes, intensificados com o prolongamento do período de armazenamento.

Produção e qualidade fisiológica de sementes de feijão-vagem em função de fontes e doses de nitrogênio

Estudaram-se os efeitos da aplicação de cinco doses e três fontes de nitrogênio sobre a produção e a qualidade fisiológica de sementes de feijão-vagem, cv. Macarrão Trepador - Hortivale, no período de setembro/2000 a fevereiro/2001, no Centro de Ciências Agrárias da Universidade Federal da Paraíba, em Areia. O delineamento experimental utilizado foi em blocos casualizados, com os tratamentos distribuídos em esquema fatorial (3 x 5) + 1, correspondendo às fontes (nitrato de cálcio, sulfato de amônio e uréia) e doses (0, 25, 50, 75 e 100kg.ha-1) de nitrogênio, e um tratamento adicional sem adubação (testemunha), com quatro repetições. As fontes nitrato de cálcio e sulfato de amônio, na dose de 100kg.ha-1, e uréia, na dose de 55kg.ha-1 de N, proporcionaram as produções de sementes no feijão-vagem de 2.571, 3.219 e 2.221kg.ha-1, respectivamente. O nitrogênio, em todas as fontes, influenciou positivamente a germinação e o vigor (índice de velocidade de germinação e emergência em campo) da semente do feijão-vagem. As doses de 68,8 e 49kg.ha-1 de N, fontes nitrato de cálcio e uréia foram responsáveis pelos valores máximos para a porcentagem de germinação, 72 e 75%, respectivamente. Para a fonte sulfato de amônio ocorreu aumento linear da porcentagem de germinação, à medida que se elevaram as doses de nitrogênio, sendo que na dose de 100kg.ha-1 obteve-se um porcentual de 84%. O índice de velocidade de germinação apresentou valores mais elevados, 6,0; 7,7 e 6,9 nas doses de 49; 71 e 53kg.ha-1 de N, fontes nitrato de cálcio, uréia e sulfato de amônio, respectivamente. A emergência em campo aumentou linearmente com elevação das doses de N (fonte nitrato de cálcio), sendo a emergência máxima (70%) obtida na dose de 100 kg ha-1 de N em cada fonte. O sulfato de amônio deve ser recomendado como fonte de N, em programas de produção de sementes de feijão-vagem.

Coran.

Al-Qur'ān

Recueil de copies de pièces, pour servir à l'histoire de France, de 791 à 1610.

Contient : 1 et 2 Duché de Réthelois ; 1 « Lettre d'exemption d'aydes, accordée aux habitans du duché de Rethelois par CHARLES VI, roy de France... Paris, le 29 decembre 1405 » ; 2 « Veriffications faictes desdictes lettres patentes, tant au Chastelet de Paris qu'autres endroictz ». La vérification est faite ici par « BRUNEAU DE S. CLAIR, chevalier, maistre du scel du roy et garde de la prevosté de Paris... le sambdy 2 may... 1411 », et contient la transcription de « quatre lettres ou mandemens de messeigneurs les generaux conseillers sur le faict des aydes ordonnées pour la guerre », datées de Paris, 17 avril 1406, 12 décembre 1407, 29 janvier 1409, 2 mai 1410 ; 3 à 5 Jeanne d'Arc ; 3 « Annoblissement donné par CHARLES VII,... roy de France, à la Pucelle d'Orleans et à tous ses parens ». Mehun-sur-Yèvre, décembre 1429. En latin ; 4 Traduction des lettres d'anoblissement qui précèdent ; 5 « Lettre de ladicte PUCELLE D'ORLEANS, escrite aux Anglois, lorsqu'ils tenoient le siege devant Orleans... Escript le mardy, en la grande sepmaine » ; 6 et 7 Écosse ; 6 « Extraict des memoires envoyez d'Escosse et recueillis par le commandement du roy d'Escosse des registres des anciennes alliances d'entre les deux couronnes de France et d'Escosse », de 791 à 1523 ; 7 « Contract de mariage d'entre le roy François II et la reyne d'Escosse », Marie Stuart. Paris, le 19 avril 1558 ; 8 à 19 Contrats de mariage ; 8 « Contract de mariage de Philippe II, roy... d'Espagne, et madame Elizabeth de France ». Paris, 20 juin 1559 ; 9 « Contract de mariage d'Emanuel Philbert, duc de Savoye, et madame Marguerite de France », fille du roi François Ier. Paris, 27 juin 1559 ; 10 « Contract de mariage d'entre le roy Charles IX et madame » Élisabeth d'Autriche. Madrid, 14 janvier 1570 ; 11 « Contract de mariage d'entre Henry III, roy... de France, et madame Louise de Lorraine,.. Rheims, 14 fevrier 1575 » ; 12 « Articles de mariage d'entre le roy... Henry IV,... et la princesse Marie de Medicis,... Florence, 25 avril 1600 » ; 13 « Renonciation faicte par la reyne Marie de Medicis à la succession de ses pere et mere ». Fontainebleau, 23 mai 1601 ; 14 « Articles de mariage d'entre [Nicolas, second fils de Henri IV], duc d'Orleans, et [Marie, fille de Henri de Bourbon, duc de Montpensier], accordez... à Paris, le 14 janvier 1608 » ; 15 « Contract de mariage du roy... Louis » XIII et de l'infante d'Espagne, Anne d'Autriche, fille de Philippe III. Madrid, 22 août 1612 ; 16 à 18 Teneur des pouvoirs donnés par LOUIS XIII, MARIE DE MEDICIS et PHILIPPE III, pour la conclusion dudit contrat ; 19 « Contract de mariage de Philippe IV, roy d'Espagne, avecq... Elisabeth de France », fille de Henri IV. 25 mars 1612 ; 20 « Droicts, auctoritez et prerogatives de la couronne de France » ; 21 « Pouvoir donné par CHARLES IX, roy de France, à Mr le duc d'Anjou, son frere, pour le gouvernement et [être] son lieutenant general par tout son royaume... Paris, le 12 novembre 1567 » ; 22 à 38 Henri IV ; 22 « Acte d'abjuration d'heresies faicte par HENRY LE GRAND, lors de sa conversion, qui fut le 25 juillet 1593 » ; 23 « Serment fait par HENRY LE GRAND,... sur l'observation » des « deux traictez... de mutuelle confederation offensive et deffensive... faicte entre [lui] et... Elizabeth, royne d'Angleterre... en l'eglise S. Ouen, à Rouen, le 19 octobre 1596 » ; 24 « Acte du serment cy devant presté » ; 25 « Don de droicts seigneuriaux, faict par HENRY IV à Gabrielle d'Estrées,... A La Fere, le 24 may 1596 » ; 26 « Pouvoir donné par la reyne d'Angleterre... ELIZABETH,... au comte de Scherosbery, pour presenter à Henry IV l'ordre de la Jarretiere... Grenach, le 6 septembre 1596 » ; 27 « Acceptation faite par HENRY IV,... dud. ordre de la Jarretiere... Rouen, le 22 octobre 1596 » ; 28 « Provisions données par HENRY IV à Henry de Bourbon, prince de Condé, de la charge de gouverneur et lieutenant general en Guyenne... S. Germain en Laye, le 25 septembre 1596 » ; 29 « Lettres de legitimation données par HENRY IV, en faveur de [Catherine] Henriette [fille du roi et de Gabrielle d'Estrées]... Paris, mars 1597 » ; 30 « Pouvoir donné par HENRY IV à Gabrielle d'Estrées, pour avoir la garde noble de ladicte damoiselle Henriette,... Paris, 10 may 1597 » ; 31 « Erection du comté de Beaufort en duché et pairie de France, faicte » par le roi HENRI IV en faveur de Gabrielle d'Estrées,... Donné au camp devant Amyens, au mois de juillet 1597 » ; 32 « Reception faicte par HENRY IV de la foy et hommage faicte à S. M. par madame Gabrielle d'Estrées, à cause du duché et pairie de Beaufort. 1597 » ; 33 « Don de droicts seigneuriaux, faict par HENRY IV en faveur de madame Gabrielle, à cause de l'acquisition du duché de Beaufort. 1597 » ; 34 « Reglement pour les parties casuelles, arresté au conseil du roy, le 29 octobre 1598 » ; 35 « Declaration de la guerre contre Mr le duc de Savoye [Charles-Emmanuel] pour le marquisat de Saluces... Faict à Lyon, le 11 aoust 1600 » ; 36 « Arrest de mort donné contre Mr le duc de Biron,... Faict en parlement, le dernier jour de juillet 1602 » ; 37 « Declaration faicte par HENRY IV au proffict de S. M. angloise [le roi Jacques]... Paris, 28 avril 1610 » ; 38 « Lettre de M. le cardinal DU PERRON à Henry IV,... De Rome, ce 29 octobre 1606 » ; 39 et 40 Philippe le Bel ; 39 « Lettre du pape BONIFACE VIII à Philippe IV, roy de France... Donné à Lateran, le quatriesme des nones de decembre, l'an de N. S. 1302 » ; 40 « Responce de PHILIPPES LE BEL »

[Portrait de L.D. Crespelle, à mi-genoux, assis, de trois-quarts à gauche] : [photographie] / Non identifié

Destinataire de l'envoi : Crespelle, L.D. (18..-18..)