Revisão taxonômica do gênero Homodiaetus (Teleostei, Siluriformes, Trichomycteridae)

The genus Homodiaetus Eigenmann & Ward, 1907 is revised and four species are recognized. Its distribution is restricted to southeastern South America, from Uruguay to Paraguay river at west to the coastal drainages of Rio de Janeiro State, Brazil. Homodiaetus is currently distinguished from other genus of Stegophilinae by the combination of the following characters: origin of ventral-fin at midlength between the snout tip and the caudal-fin origin; opercle with three or more odontodes; and gill membranes confluent with the istmus. Homodiaetus anisitsi Eigenmann & Ward, 1907, is diagnosed by the caudal-fin with black middle rays, margin of upper and lower procurrent caudal-fin rays with dark stripes extending to the caudal-fin, and 3-6 opercular odontodes; H. passarellii (Ribeiro, 1944) with 6-7 opercular odontodes, 21-24 lower procurrent caudal-fin rays and 23-26 upper procurrent caudal-fin rays; H. banguela sp. nov. with 9 opercular odontodes, 17-19 lower procurrent caudal-fin rays, 17-22 upper procurrent caudal-fin rays, reduction of fourth pharyngobranchial with only three teeth and untoothed fifth ceratobranchial; and H. graciosa sp. nov. with 5-6 dentary rows, 7-9 opercular odontodes and 16-23 upper procurrent caudal-fin rays.

A new species of Tmesiphantes (Araneae, Theraphosidae) from the state of Pará, Brazil

A new species of Tmesiphantes Simon, 1892, T. aridai sp. nov., is described and illustrated based on three males and three females collected at the Floresta Nacional do Tapajós, Santarém, state of Pará, Brazil. Males can be distinguished from T. perp Guadanucci & Silva, 2012 by the palpal bulb with an inconspicous tegular basal projection, but presenting a very slender embolus with shorter keels not extending to the tip, and from T. nubilus Simon, 1892 by the tibial apophysis with two similarly sized branches, the prolateral one with a strong spine on the retrolateral margin. Females resemble T. nubilus by the aspect of the seminal receptacle but the constriction near the apex is less evident and apex shape is irregular. The new species represents the first record of Tmesiphantes from the Amazonian region, bringing the total number of species to eight.

Incomplete excision of basal cell carcinoma in the subunits of the nose.

Reconstructive procedures after resection of nasal basal cell carcinoma (BCC) vary depending on the subunit involved. The aim of the present study was to assess the influence of the location of the BCC on the rate of incomplete excisions, so we made a retrospective analysis of all nasal BCC excised at our hospital between 2002 and 2005. The incomplete excision rate was 24/148 (16%). More incomplete excision occurred on the alae (n=13) when compared to the dorsum (n=2) of the nose (p<0.05). Eight two-staged procedures resulted in incomplete resection, whereas 9 (6%) frozen section analyses were false-negative. BCC were most likely to be incompletely excised on the nasal tip and alae, and both subunits required more elaborate reconstructions. This, however, was not the result of poor estimation of the extent of the tumour and reluctance to excise more challenging areas widely for reconstruction, but to the method chosen to eradicate the tumour.

Use of GPS for Photogrammetry, HR-342, 1993

Five test flights were conducted to study the use of Global Positioning System (GPS) in Photogrammetry, three in Iowa, one each in California and Texas. These tests show that GPS can be used to establish ground control by the static method and to determine camera location by the kinematic method. In block triangulation, six GPS controls are required and additional elevation control along the centerline is also required in strip triangulation. The camera location determined by aerial triangulation depends on the scale of the photography. The 1:3000 scale photography showed that the absolute accuracy of the camera location by GPS is better than five centimeters. The 1:40000 scale photography showed that the relative accuracy of the camera location by GPS is about one millimeter. In a strip triangulation elevation control is required in addition to the camera location by GPS. However, for block triangulation camera location by GPS is sufficient. Pre-targeting of pass and tie points gives the best results in both block and strip triangulation. In normal mapping for earth work computations the use of 1:6000 scale photography with GPS control instead of 1:3000 scale is recommended. It is recommended that research be done in the use of GPS for navigation in aerial photographic missions. It is highly recommended that research be done in the use of GPS to determine tip and tilt of the aerial camera, that is required in stereoplotting.

Fuzzy logic algorithm to extract specific interaction forces from atomic force microscopy data

The atomic force microscope is not only a very convenient tool for studying the topography of different samples, but it can also be used to measure specific binding forces between molecules. For this purpose, one type of molecule is attached to the tip and the other one to the substrate. Approaching the tip to the substrate allows the molecules to bind together. Retracting the tip breaks the newly formed bond. The rupture of a specific bond appears in the force-distance curves as a spike from which the binding force can be deduced. In this article we present an algorithm to automatically process force-distance curves in order to obtain bond strength histograms. The algorithm is based on a fuzzy logic approach that permits an evaluation of "quality" for every event and makes the detection procedure much faster compared to a manual selection. In this article, the software has been applied to measure the binding strength between tubuline and microtubuline associated proteins.

Maize leaf development under climate change scenarios

The objective of this work was to simulate maize leaf development in climate change scenarios at Santa Maria, RS, Brazil, considering symmetric and asymmetric increases in air temperature. The model of Wang & Engel for leaf appearance rate (LAR), with genotype-specific coefficients for the maize variety BRS Missões, was used to simulate tip and expanded leaf accumulated number from emergence to flag leaf appearance and expansion, for nine emergence dates from August 15 to April 15. LAR model was run for each emergence date in 100-year climate scenarios: current climate, and +1, +2, +3, +4 and +5°C increase in mean air temperature, with symmetric and asymmetric increase in daily minimum and maximum air temperature. Maize crop failure due to frost decreased in elevated temperature scenarios, in the very early and very late emergence dates, indicating a lengthening in the maize growing season in warmer climates. The leaf development period in maize was shorter in elevated temperature scenarios, with greater shortening in asymmetric temperature increases, indicating that warmer nights accelerate vegetative development in maize.

Particle detector link system modifications in RE1/1 sector

The large hadron collider constructed at the European organization for nuclear research, CERN, is the world’s largest single measuring instrument ever built, and also currently the most powerful particle accelerator that exists. The large hadron collider includes six different experiment stations, one of which is called the compact muon solenoid, or the CMS. The main purpose of the CMS is to track and study residue particles from proton-proton collisions. The primary detectors utilized in the CMS are resistive plate chambers (RPCs). To obtain data from these detectors, a link system has been designed. The main idea of the link system is to receive data from the detector front-end electronics in parallel form, and to transmit it onwards in serial form, via an optical fiber. The system is mostly ready and in place. However, a problem has occurred with innermost RPC detectors, located in sector labeled RE1/1; transmission lines for parallel data suffer from signal integrity issues over long distances. As a solution to this, a new version of the link system has been devised, a one that fits in smaller space and can be located within the CMS, closer to the detectors. This RE1/1 link system has been so far completed only partially, with just the mechanical design and casing being done. In this thesis, link system electronics for RE1/1 sector has been designed, by modifying the existing link system concept to better meet the requirements of the RE1/1 sector. In addition to completion of the prototype of the RE1/1 link system electronics, some testing for the system has also been done, to ensure functionality of the design.

Patch-clamp detection of macromolecular translocation along nuclear pores

The present paper reviews the application of patch-clamp principles to the detection and measurement of macromolecular translocation along the nuclear pores. We demonstrate that the tight-seal 'gigaseal' between the pipette tip and the nuclear membrane is possible in the presence of fully operational nuclear pores. We show that the ability to form a gigaseal in nucleus-attached configurations does not mean that only the activity of channels from the outer membrane of the nuclear envelope can be detected. Instead, we show that, in the presence of fully operational nuclear pores, it is likely that the large-conductance ion channel activity recorded derives from the nuclear pores. We conclude the technical section with the suggestion that the best way to demonstrate that the nuclear pores are responsible for ion channel activity is by showing with fluorescence microscopy the nuclear translocation of ions and small molecules and the exclusion of the same from the cisterna enclosed by the two membranes of the envelope. Since transcription factors and mRNAs, two major groups of nuclear macromolecules, use nuclear pores to enter and exit the nucleus and play essential roles in the control of gene activity and expression, this review should be useful to cell and molecular biologists interested in understanding how patch-clamp can be used to quantitate the translocation of such macromolecules into and out of the nucleus

Fabrication des films microstructurés et leurs caractéristiques en spectroscopie de résonance des plasmons de surface

Cette thèse caractérise les propriétés optiques des matériaux plasmoniques microstructurés et procède à l’évaluation des paramètres analytiques afin de les employer comme plateforme de biodétection en spectroscopie de résonance des plasmons de surface (SPR). Aux dimensions micrométriques, les matériaux plasmoniques présentent des caractéristiques optiques propres aux nano- et macromatériaux. La cartographie physicooptiques en SPR de matériaux méso- et microscopiques s’est effectuée à l’aide de films structurés de motifs périodiques triangulaires et circulaires fabriqués par une technique modifiée de lithographie par nanosphères (nanosphere lithography, NSL). À partir de cette vue d’ensemble, quelques films structurés ont été sélectionné en fonction d’aspects analytiques tels que la sensibilité et la résolution face aux variations d’indice de réfraction (RI) pour déterminer le potentiel de ces matériaux comme plateforme de biodetection. Les propriétés optiques distinctes des films microstructurés proviennent d’interactions résonantes entre les modes de plasmons de surface (SP) localisé et délocalisé identifiés par la relation de dispersion en SPR ainsi que l’imagerie Raman. Les conditions de résonance des modes SP dépendant de paramètres expérimentaux (λ, θ, η) tel qu’observés numériquement par rigorous coupled wave analysis (RCWA) et empiriquement. Ces travaux démontrent la nature plasmonique distincte des micro-matériaux et leur potentiel d’intégration aux techniques analytiques SPR existantes. Les matériaux plasmoniques micrométriques furent également étudiés pour l’implémentation de la SPR à une pointe de microscopie à force atomique (atomic force microscopy, AFM) combinant ainsi la spectroscopie à l’imagerie topographique. Des travaux préliminaires se sont concentrés sur la signature spectroscopique de leviers en silicium (Si) et en nitrure de silicium (Si3N4), l’impact d’un revêtement d’or sur les pointes et l’influence de milieu environnant. Une image d’origine plasmonique a été obtenue avec des leviers en Si3N4 revêtus d’or en transmission dans un environnement aqueux, indiquant ainsi le potentiel de ces pointes comme micro-biocapteur SPR. Ces résultats préliminaires servent de fondement pour orienter les prochaines investigations dans ce projet.

Non-linear mechanical behavior of the elastomer polydimethylsiloxane (PDMS) used in the manufacture of microfluidic devices

Polydimethylsiloxane (PDMS) is the elastomer of choice to create a variety of microfluidic devices by soft lithography techniques (eg., [1], [2], [3], [4]). Accurate and reliable design, manufacture, and operation of microfluidic devices made from PDMS, require a detailed characterization of the deformation and failure behavior of the material. This paper discusses progress in a recently-initiated research project towards this goal. We have conducted large-deformation tension and compression experiments on traditional macroscale specimens, as well as microscale tension experiments on thin-film (≈ 50µm thickness) specimens of PDMS with varying ratios of monomer:curing agent (5:1, 10:1, 20:1). We find that the stress-stretch response of these materials shows significant variability, even for nominally identically prepared specimens. A non-linear, large-deformation rubber-elasticity model [5], [6] is applied to represent the behavior of PDMS. The constitutive model has been implemented in a finite-element program [7] to aid the design of microfluidic devices made from this material. As a first attempt towards the goal of estimating the non-linear material parameters for PDMS from indentation experiments, we have conducted micro-indentation experiments using a spherical indenter-tip, and carried out corresponding numerical simulations to verify how well the numerically-predicted P(load-h(depth of indentation) curves compare with the corresponding experimental measurements. The results are encouraging, and show the possibility of estimating the material parameters for PDMS from relatively simple micro-indentation experiments, and corresponding numerical simulations.

Klebsiella pneumoniae subsp. pneumoniae–bacteriophage combination from the caecal effluent of a healthy woman

A sample of caecal effluent was obtained from a female patient who had undergone a routine colonoscopic examination. Bacteria were isolated anaerobically from the sample, and screened against the remaining filtered caecal effluent in an attempt to isolate bacteriophages (phages). A lytic phage, named KLPN1, was isolated on a strain identified as Klebsiella pneumoniae subsp. pneumoniae (capsular type K2, rmpA+). This Siphoviridae phage presents a rosette-like tail tip and exhibits depolymerase activity, as demonstrated by the formation of plaque-surrounding haloes that increased in size over the course of incubation. When screened against a panel of clinical isolates of K. pneumoniae subsp. pneumoniae, phage KLPN1 was shown to infect and lyse capsular type K2 strains, though it did not exhibit depolymerase activity on such hosts. The genome of KLPN1 was determined to be 49,037 bp (50.53 %GC) in length, encompassing 73 predicted ORFs, of which 23 represented genes associated with structure, host recognition, packaging, DNA replication and cell lysis. On the basis of sequence analyses, phages KLPN1 (GenBank: KR262148) and 1513 (a member of the family Siphoviridae, GenBank: KP658157) were found to be two new members of the genus “Kp36likevirus”.

Estudo comparativo entre a subvenção econômica à inovação operada pela Finep e programas correlatos de subsídio em países desenvolvidos

A inovação tecnológica é um dos principais fatores de competitividade das economias modernas e, em todos os países desenvolvidos, é objeto de políticas oficiais para sua promoção. No Brasil, a Financiadora de Estudos e Projetos (Finep) apóia o desenvolvimento tecnológico das empresas desde 1976. A partir de 2006, passou a operar também subsídios diretos na forma de subvenção econômica. Este estudo caracteriza a subvenção econômica no marco legal brasileiro, apresentando exemplos de sua utilização como política pública e, especificamente, a subvenção à inovação operada pela Finep no período de 2006 a 2009. Em seguida, o instrumento da Finep é comparado, por análise de conteúdo, com programas de subsídio correlatos em países desenvolvidos. Para tal, foram selecionados os programas norte americanos SBIR, ATP e TIP, e aqueles operados pela OSEO na França e CDTI na Espanha. São abordados aspectos relativos à lógica de intervenção, dando ênfase a questões como risco tecnológico, intensidade no apoio, modelos institucionais e integração de instrumentos de apoio.

Análise de vizinhança na avaliação de genótipos de cana-de-açúcar

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Lesões desportivas no atletismo: comparação entre informações obtidas em prontuários e inquéritos de morbidade referida

Para compreender as lesões desportivas (LD) é necessário quantificá-las, associando-as a fatores causais particulares ao esporte. Contudo, faltam registros sobre tais agravos nas instituições esportivas, sobretudo no atletismo brasileiro, em que poucos clubes possuem serviços de assistência à saúde. Na ausência de tais registros, estudos na área de saúde pública, utilizam-se de outros recursos epidemiológicos para coletas, tais como os inquéritos de morbidade referida. A partir dessa escassez de informações e a facilidade de obtenção de dados junto aos próprios atletas, objetivou-se, para esta pesquisa, levantar informações sobre LD referidas por atletas de alto rendimento, retrocedendo em oito meses, e compará-las com os registros de prontuários clínicos. Para tanto, foram tomados 25 atletas de elite, 16 do gênero masculino e nove do feminino, com idade de 25,7 ± 4,4 anos, altura de 1,74 ± 0,10m, peso 70,4 ± 13,15kg e tempo médio de treinamento de 8,38 ± 4,06 anos. Dois fisioterapeutas foram treinados separadamente para coletar informações sobre LD. Um deles em prontuários e o outro dos próprios atletas, através de entrevista (inquéritos de morbidade referida - IMR). Estudo de concordância de respostas para as duas formas de coleta foi realizado pelo teste da proporção binomial, estabelecendo-se limites de 95% de confiança para a concordância. Os resultados mostraram que em todas as variáveis estudadas os valores estavam dentro dos limites de confiança estabelecidos pelos testes estatísticos, sendo: 88,33% para as variáveis tipo de lesão ou agravo e mecanismo de lesão ou aumento dos sintomas, 90% para a variável qualidade do retorno às atividades desportivas e 91,67% para as variáveis local anatômico e período de treinamento. Concluiu-se que houve elevada taxa de concordância entre as informações levantadas, mostrando a eficácia do IMR para a coleta de informações sobre lesões desportivas para a população investigada.

Scanning tunneling microscope operating as a spin diode

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)