53 resultados para raina
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Back Row: manager Stephanie Van Eyk, student trainer Jennifer Rasmussen, trainer Roxann Dahl, Ruth Kipping, Raina Goodlow, Mandy Stowe, head coach Sue Guevara, Katie Dykhouse, Alison Miller, Heather Oesterle, manager James Sanders, manager Yulonda Cole, manager Robyn Scherr
Front Row: ass't coach Eileen Shea, vol. ass't coach Molly Murray, Alayne Ingram, Kenisha Walker, Ann Lemire, Stacey Thomas, Anne Thorius, ass't coach Yulonda Wimbish, ass't coach Yvette Harris
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Back Row: ass't coach Yvette Harris, ass't coach Eileen Shea, Alayne Ingram, Infini Robinson, Susana Jara, Ruth Kipping, LeeAnn Bies, Raina Goodlow, Heather Oesterle, manager James Sanders, ass't coach Yulonda Wimbish-Gay, head coach Sue Guevara
Front Row: manager Yolanda Cole, trainer Roxann Dahl, co-captain Anne Thorius, Alison Miller, co-captain Stacey Thomas, Kenisha Walker, Katie Dykhouse, student trainer Jamie Corl, manager Stephanie Van Eyk
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Back Row: ass't coach Ron Mott, ass't coach Yvette Harris, Cathy Speck, Leeann Bies, Jennifer Smith, Raina Goodlow, Christie Schumacher, James Sanders, dir. basketball operations Yulonda Wimbish-Gay, ass't coach Eileen Shea
Middle Row: trainer Roxann Dahl, manager Joy Huntington, Infini Robinson, Stephanie Gandy, Alayne Ingram, Michaela Leary, Susan Jara, Heather Oesterle, head coach Sue Guevara
Front Row: Anne Thorius, Katie Dykhouse
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Leeann Bies, Jennifer Smith, Heather Oesterle, Raina Goodlow, Katie Dykhouse, Christie Schumacher
Front Row: Anne Thorius, Stephanie Gandy, Alayne Ingram, Michaela Leary, Infini Robinson, Susana Jara
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Back Row: head coach Sue Guevara, assistant head coach Angela Jackson, Tabitha Pool, BreAnne McPilamy, Katrina Mason, Jennifer Smith, LeeAnn Bies, Stephanie Gandy, Sierra Hauser-Price, assistant coach Eileen Shea-Hilliard, assistant coach Ron Mott,
director of basketball operations Molly Murray, trainer Roxann Dahl, Alayne Ingram, Heather Oesterle, Raina Goodlow, Susana Jara, manager Stacey Lerchenfeld, student trainer Beth Chase
Resumo:
Dsb proteins control the formation and rearrangement of disulfide bonds during the folding of secreted and membrane proteins in bacteria. DsbG, a member of this family, has disulfide bond isomerase and chaperone activity. Here, we present two crystal structures of DsbG at 1.7- and 2.0-Angstrom resolution that are meant to represent the reduced and oxidized forms, respectively. The oxidized structure, however, reveals a mixture of both redox forms, suggesting that oxidized DsbG is less stable than the reduced form. This trait would contribute to DsbG isomerase activity, which requires that the active-site Cys residues are kept reduced, regardless of the highly oxidative environment of the periplasm. We propose that a Thr residue that is conserved in the cis-Pro loop of DsbG and DsbC but not found in other Dsb proteins could play a role in this process. Also, the structure of DsbG reveals an unanticipated and surprising feature that may help define its specific role in oxidative protein folding. Thus, the dimensions and surface features of DsbG show a very large and charged binding surface that is consistent with interaction with globular protein substrates having charged surfaces. This finding suggests that, rather than catalyzing disulfide rearrangement in unfolded substrates, DsbG may preferentially act later in the folding process to catalyze disulfide rearrangement in folded or partially folded proteins.
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2000 Mathematics Subject Classification: 60G15, 60G60; secondary 31B15, 31B25, 60H15
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Dissertação (mestrado)—Universidade de Brasília, Instituto de Ciências Humanas, Departamento de Geografia, 2016.