Use of gastric balloon manometry for estimation of intra-abdominal pressure in horses

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Comparação de métodos para determinar biomassa na região amazônica

The aboveground biomass content of a region can be estimated by either direct or indirect methods. Direct methods correspond to the biomass content determination with scales and extrapolation of results to larger areas. It is a destructive and very laborious procedure. Indirect methods utilize formulas whose entrance parameters are obtained from forest inventories. Forest inventories are made with the purpose to plan exploration and land use and the inventory data are frequently not suitable for biomass estimation. Problems with both methods increase in the Amazon region, where little information is available on forest biomass. The objective of this paper is to establish, by comparing the application of the indirect and direct methods in the determination of the biomass, the more appropriate indirect formulation to represent the characteristic vegetation of a region in the amazonian forest. A 0.2 hectare area was chosen, which was part of a major forest clearing experiment conducted in Tomé Açu, a town located 250 km south of Belém, the capital of the Brazilian state of Pará. The entire biomass in the area was weighted with scales during the three weeks that followed the cut of the forest in July 1994. A detailed inventory was carried out in the area and then the indirect method was applied in the data. Seven different formulas for determining biomass were used. Comparison of the data of real mass and the mass obtained through the application of the seven formulas indicated that the more suitable for the region is given by FW = α · φβ · Hγ, where FW is total fresh weight (kg), φ is the diameter at breast height (cm), H is the total height of the tree and α, β and γ are regression coefficients (equal to 0.026, 1.529 and 1.747, respectively).

Soroepidemiologia da babesiose em rebanho de bovinos da raça Curraleiro

Bovine babesiosis is a blood parasitic disease. In Brazil it is caused by B. bovis and B. bigemina protozoa, both of which reveal the Boophilus microplus tick as the only biological vector. Animal samples were collected at Experimental Study Farm of Curraleiro Cattle (ESFC) in 2001 (n=117) and 2003 (n=113). The detection of antibodies against B. bovis and B. bigemina was carried out by ELISA-indirect method. This research was aimed at studing seroepidemiological aspects of bovine babesiosis in a Curraleiro herd, as well as obtain information about babesiosis stability in this population and relate the results with available climactic and management information. The occurrence rate of positive animals was 92.3% for B. bovis and 83.8% for B. bigemina in 2001; in 2003 it was 92.9% and 66.4%, respectively. There was a significant difference between seropositive frequency and age in 2003; such a frequency decreased with ageing. It was possible to conclude that despite environmental conditions and chemical controls against endo and ectoparasites, these animals were exposed to Babesia spp and they found themselves in a situation of enzootic stability for babesiosis.

Immunohistochemistry assay to detect Turkey coronavirus (TCoV) from experimentally infected poults

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Metodologia para quantificação de leucóciotos totais em peixe Oreochromis Niloticus

Haematological investigation is an important part of disease diagnosis. The techniques used for mammals are generally applicable for fishes with slight modification. The presence of nucleated erythrocytes and thrombocytes in fish may cause some confusion in the identification of blood cells, mainly in total leukocytes count. This work evaluated two different methods (direct and indirect) of leukocytes counting. Specimens of tilapia were divided randomly into four groups of 48 fish each. Each group was further randomized into three replicate experiments of 16 fish per replicate in 40 L aquarium and maintained for 10 days. Counting of leukocytes in peripheral blood was determined in six fishes per group at the times: 0, 3, 7 and 10 days. The direct method in Neubauer chamber showed a large dispersion of data in regard to the average, the opposite was observed in indirect method determined in blood smears that showed more homogeneity among groups. Due to this factor the indirect method can be considered more accurate than the direct method for counting leukocytes in fishes.

Fezes equina como fonte de inóculo na obtenção de indicadores indigestíveis para estimar a digestibilidade em equinos

Four castrated horses were utilized in randomized blocks experimental design. The objective was to study the in vitro acquirement of indigestible fiber, neutral detergent fiber (NDFi) and acid detergent fiber (ADFi), using as inoculum source rumen fluid (RF) or horse feces (HF), to estimate the nutrient digestibility (ND) in horses. Treatments consisted of direct method by total feces collection (TC) and indirect method either by the use of NDFi or ADF by the use of LR or HF as inocula source. The first essay was done with horses fed coast-cross hay exclusively, while for the essay two, the horses were fed of 70% of coast-cross hay and 30% corn grain. For the essay 1, ADFi-HF presented the best marker recuperation rate (RR) (103.67%), being similar to control (TC), while NDFi-RF and ADFi-RF resulted in lower RR (P<0.05), 83.43 and 88.28%, respectively. The ND was adequately estimated by NDFi-HF and ADFi-HF. On the second essay there were no significant effects of the marker type, as well as for the indigestible marker acquirement method, for the marker RR (average value of 101%). The ND was adequately predicted by the ADFi obtained for both innocula source and NDFi obtained by the use of HF as inoculum, for horses fed a mixed diet. It can be concluded that horse feces may be used as inoculum source for the acquirement of NDFi and ADFi in vitro for digestibility determination on horses fed coastcross hay exclusively, as well as for horses fed a mixed diet.

Predição da potência aeróbia (VO 2máx) de crianças e adolescentes em teste incremental na esteira rolante

The maximal oxygen uptake (VO2max) is the maximal quantity of energy that can be produced by the aerobic metabolism in certain time unity. It can be determined direct or indirectly by predictive equations. The objective of this study was to make a specific predictive equation to determine the VO 2max from boys aged 10-16 years-old. Forty-two boys underwent a treadmill running ergospirometric test, with the initial velocity set at 9 km/h, until voluntary exhaustion. By the multiple linear regression was possible to develop the following equation for the indirect determination of the VO 2max: VO2max (ml/min) = -1574.06 + (141.38 x Vpeak) + (48.34 * Body mass), with standard error of estimate = 191.5 ml/min (4.10 ml/kg/min) and coefficient of determination = 0.934. We suggest that this formula is appropriate to predict VO2max for this population.

Avaliação do sistema radicular da soja sob sistemas de manejo do solo

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Caracterização do antígeno-5, alérgeno do veneno da vespa social Polybia paulista com uma abordagem proteômica

Pós-graduação em Ciências Biológicas (Biologia Celular e Molecular) - IBRC

Influência do pneumoperitôneo sobre a pressão pleural, parâmetros cardiovasculares e hemogasométricos em eqüinos

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Expressão da proteína S1 recombinante do vírus da bronquite infecciosa em Saccharomyces cerevisiae para aplicação no imunodiagnóstico

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Valor nutritivo da silagem de grãos úmidos de milho na alimentação de vacas da raça holandesa

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Efeito do tratamento crônico com fluoreto na atividade salivar, ossos e dentes de ratos espontaneamente hipertensos (SHR)

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Efeitos da membrana amniótica homógena associada ao laser arseneto de gálio-alumínio (λ=660) na reparação de mucosite oral em ratos tratados com 5-fluoruracila: análise histológica e imunohistoquímica

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Projeto de experimentos em rodas ferroviárias fundidas em aço, com o uso da técnica de Taguchi

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)