The production of algae growing on gravel in a chalk-stream

Epilithic algae, ie that growing on the surface of stones, was studied as part of the work on the energy flow of the chalk-stream ecosystem, by the River Laboratory. The study area was on Bere Stream and 2 neighbouring streams. The algal biomass was estimated from analysis of chlorophyll a. In Bere Stream the peak chlorophyll a cover occurred in April, while in the neighbouring streams, which have considerably lower nutrient levels, there was on peak. Assuming that 2% of a diatoms dry wt is chlorophyll a, then even in mid-April the biomass of epilithic algae amounted to no more than 15 g dry wt m Super(-2) of exposed gravel. Annual production was calculated to be > 15 times greater than biomass. The estimation of net primary production is always difficult for benthic floras and comparisons are especially difficult when different methods are used. But these figures contrast sharply with those for Ranunculus (water crowfoot) which has a ratio of annual production to maximal seasonal biomass of 1:16. The accumulation of algal biomass is apparently being prevented. Some organic matter may be excreted; some algae will be washed off the bed of the stream by current and grazing by herbivorous invertebrates will also tend to prevent algal accumulation.

Experiments using an artificial stream to investigate the seasonal growth of chalk-stream algae

Research into the production ecology of chalk streams using a large artificial recirculating stream is described. Physical chemical processes including calcium and inorganic phosphate levels, and exchange of gaseous carbon dioxide in both a simple closed system and a circulating system with gravel substrate have been monitored in both light and dark conditions. Further experiments were concerned with the seasonal changes in algal growth over the gravel substrate with constant water velocities and replenishment. The algal population, composed mainly of the diatoms Achnanthes minutissima, Meridion circulare, Nitzschia fonticola and Synedra ulna reached a peak in mid May and declined rapidly during June. Concentrations of phosphate phosphorus fell as the diatoms grew but was not thought to limit growth. Silicate concentrations followed the diatom cycle closely but never fell below 0.8 mg/l Si. It is possible that one of the nutrients may have been limiting the rate of growth due to steep diffusion gradients through the algal mat. In the last summer and autumn a hard calcareous crust composed of the green alga Gongrosira incrustans and the blue green alga Homeothrix varians , developed. The channel stream is compared with the natural conditions found in chalk streams.

Automatic identification and enumeration of algae

A good understanding of the population dynamics of algal communities is vital in many ecological and pollution studies of freshwater and oceanic systems. Present methods require manual counting and identification of algae and can take up to 90 min to obtain a statistically reliable count on a complex population. Several alternative techniques to accelerate the process have been tried on marine samples but none have been completely successful because insufficient effort has been put into verifying the technique before field trials. The objective of the present study has been to assess the potential of in vivo fluorescence of algal pigments as a means of automatically identifying algae. For this work total fluorescence spectroscopy was chosen as the observation technique.

Methods of determining the quality of water using the functional characteristics of algae

In the study of questions relating to the quality of raw water and the biological produc- tivity of water bodies algal indicators have an important place. Despite the importance of these functional indicators in determining the quality of water and the nature of the production processes as a basis for preserving the ecological equilibrium of aquatic ecosystems, their use in the system of hydrobiological methods of monitoring the quality of surface water has not received proper consideration. This paper aims to analyse the matter and the possibl use of functional algal criteria in the system for the biological monitoring of aquatic objects and also to give some results in using these criteria.

Eutrophication and the management of planktonic algae: What Vollenweider couldn't tell us

The ”Vollenweider model” is a sophisticated mathematical statement about the long-range behaviour of (mainly temperate) lakes and their ability to support phytoplankton chlorophyll. Misapplication of the model, against which Vollenweider himself warned, has led to many misconceptions about the dynamics of plankton in lakes and reservoirs and about how best to manage systems subject to eutrophication. This contribution intends to frame the most important issues in context of the phosphorus- loading and phosphorus-limitation concepts. Emphasis is placed on the need to distinguish rate-limitation from capacity-limitation, to understand which is more manageable and why, to discern the mechanisms of internal recycling and their importance, and to appreciate the respective roles of physical and biotic components in local control of algal dynamics. Some general approaches to the management of water quality in lakes and reservoirs to eutrophication are outlined.

Calcite covering of sediment as a possible way of curbing blue-green algae

Natural calcite precipitation in lakes is a well-known control mechanism of eutrophication. In hard-water lakes, calcite deposits on the flat bottoms of shallow lakes and near the shores of deeper lakes resulted from biogenic decalcification during the millenia after the last glacial period. The objective of a new restoration technology is to intensify the natural process of precipitation by utilizing the different qualities of calcareous mud layers. In a pilot experiment in Lake Rudower See, East Germany, phosphorus-poor deeper layers of the sediments were flushed out and spread over the phosphorus-rich uppermost sediments, to promote the co- precipitation of calcite with phosphorus from the water-column.

Immunodetection of planktonic algae

Interest in the identification and characterisation of cyanobacteria and dinoflagellates in aquatic environments is increasing rapidly due to the perceived roles of these organisms in primary production and nuisance aspects in terms of water treatment and public health. Techniques for the identification and quantification of these organisms currently are limited, and the application of molecular approaches provides fundamental taxonomic information and techniques of practical value. Antigenic properties of algal cells may be useful taxonomic markers. Immunodetection techniques utilise the specificity of the antibody/antigen association as a probe for recognising and distinguishing between microorganisms according to their cell- surface chemistry. Immunofluorescent detection of unicellular cyanobacteria and dinoflagellates has been studied with success in marine and freshwater ecosystems and a range of techniques and results are presented and discussed. The most recent advances in the study of planktonic algae have come with the application of continuous flow cytometric methods (CFC). Flow cytometry makes use of the autofluorescence properties of the algal cells, which alone can be used to demonstrate their presence and permit their quantification in natural water samples. When used in conjunction with immunolabelling techniques, the potential of CFC analysis is broadened to study the serological/strain composition of plankters in natural populations. Changes in algal strains represented within and between waters over periods of time are reported and discussed, along with the ecological issues thus raised.

Field applications of the second-generation Environmental Sample Processor (ESP) for remote detection of harmful algae: 2006-2007

We assess the application of the second-generation Environmental Sample Processor (ESP) for the detection of harmful algal bloom (HAB) species in field and laboratory settings using two molecular probe techniques: a sandwich hybridization assay (SHA) and fluorescent in situ hybridization (FISH). During spring 2006, the first time this new instrument was deployed, the ESP successfully automated application of DNA probe arrays for various HAB species and other planktonic taxa, but non-specific background binding on the SHA probe array support made results interpretation problematic. Following 2006, the DNA array support membrane that we were using was replaced with a different membrane, and the SHA chemistry was adjusted. The sensitivity and dynamic range of these modifications were assessed using 96-well plate and ESP array SHA formats for several HAB species found commonly in Monterey Bay over a range of concentrations; responses were significantly correlated (p < 0.01). Modified arrays were deployed in 2007. Compared to 2006, probe arrays showed improved signal:noise, and remote detection of various HAB species was demonstrated. We confirmed that the ESP and affiliated assays can detect HAB populations at levels below those posing human health concerns, and results can be related to prevailing environmental conditions in near real-time.

Algae and water pollution in Mahi Estuary

Distribution of phytoplankton, chlorophyll A and phaeophytin was studied at different locations in the Mahi Estuary, Gujarat, India during 1982. The water quality at the discharge point was poor while the region away from it was relatively unpolluted. The results indicated a wide range of variation in phytoplankton population (7.68-5010, 96 x 10 super(4) cells/l, chl. a 2.22-58.22 mg/m super(3) and phaeophytin [0.20-10.21 mg/m super(3)]. The ratio of chl. a/phaeophytin were remarkably low at highly polluted stations. Higher abundance of the genera Oscillatoria and Nitzschia were recorded at polluted stations. The diversity of species at the unpolluted station was higher (1.56) as compared to the polluted station (1.07).

Sterol composition of marine algae from Karachi coast of Arabian Sea

During the course of chemical investigation of marine algae collected from Karachi coast of Arabian Sea, five sterols named as sarangosterol(1), 23-methyl cholesta-5, 25-dien-3ß-ol(2) from Endarachne binghamiae (brown alga), sargasterol(3) from Dictyota indica (brown alga), cholesterol(4) from Laurencia obtusa (red alga) and clerosterol(5) from Codium iyengarii (green alga) have been isolated. Their structures were elucidated with the help of spectroscopic means.