Honey bee lines selected for high propolis production also have superior hygienic behavior and increased honey and pollen stores

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Characterization of cuticular hydrocarbons of diploid and haploid males, workers and queens of the stingless bee Melipona quadrifasciata

Males, queens and workers of stingless bees show differences in external morphology, behaviour and roles within a colony. In addition, each individual has a cuticular chemical signature responsible for mutual communication that is essential for maintaining the integrity of the colony. In this paper we characterize the cuticular hydrocarbon composition of newly emerged diploid and haploid males, workers and virgin queens of Melipona quadrifasciata by gas chromatography-mass spectrometry (GC/MS) analysis. This is the first time that the cuticular profile of diploid males in a species of stingless bee has been characterized. We found differences in the cuticular hydrocarbon composition among males, workers and virgin queens, recording both qualitative and quantitative differences among individuals of different phenotypes. However, no compound was found exclusively in diploid males. The cuticular chemical profiles of haploid and diploid males were very similar to those of workers. Moreover, the cuticular lipids of males and workers were significantly different from those of queens. Tricosane, pentacosene-2 and 7-methyl-heptacosane were the compounds responsible for this significant separation. This result correlates with the behavioural and morphological differences among these phenotypes.

Gene copy number and differential gene expression in haploid and diploid males of the stingless bee, Melipona quadrifasciata

Complementary sex determination in Hymenoptera implies that heterozygosity at the sex locus leads to the development of diploid females, whereas hemizygosity results in haploid males. Diploid males can arise through inbreeding. In social species, these pose a double burden on colony fitness, from significant reduction in its worker force and through being less viable and fertile than haploid males. Apart from being "misfits", diploid males are of interest to assess molecular correlates for possibly ploidy-related bionomic differences. Herein, we generated suppression subtractive cDNA libraries from newly emerged haploid and diploid males of the stingless bee Melipona quadrifasciata to enrich for differentially expressed genes. Gene Ontology classification revealed that in haploid males more DEGs were related to stress responsiveness, biosynthetic processes, reproductive processes and spermatogenesis, whereas in diploid ones differentially expressed genes were associated with cellular organization, nervous system development and amino acid transport were prevalent. Furthermore, both libraries contained over 40 % ESTs representing possibly novel transcripts. Quantitative RT-PCR analyses confirmed the differential expression of a representative DEG set in newly emerged males. Several muscle formation and energy metabolism-related genes were under-expressed in diploid males. On including 5-day-old males in the analysis, changes in transcript abundance during sexual maturation were revealed.

Rapid morphological changes in populations of hybrids between Africanized and European honey bees

African honey bees, introduced to Brazil in 1956, rapidly dominated the previously introduced European subspecies. To better understand how hybridization between these different types of bees proceeded, we made geometric morphometric analyses of the wing venation patterns of specimens resulting from crosses made between Africanized honey bees (predominantly Apis mellifera scutellata) and Italian honey bees (A. mellifera ligustica) from 1965 to 1967, at the beginning of the Africanization process, in an apiary about 150 km from the original introduction site. Two virgin queens reared from an Italian parental were instrumentally inseminated with semen from drones from an Africanized parental. Six F-1 queens from one of these colonies were open mated with Africanized drones. Resultant F-1 drones were backcrossed to 50 Italian and 50 Africanized parental queens. Five backcross workers were collected from each of eight randomly selected colonies of each type of backcross (N = 5 bees x 8 colonies x 2 types of backcrosses). The F-1 progeny (40 workers and 30 drones) was found to be morphologically closer to the Africanized than to the European parental (N = 20 drones and 40 workers, each); Mahalanobis square distances = 21.6 versus 25.8, respectively, for the workers, and 39.9 versus 46.4, respectively, for the drones. The worker progenies of the backcrosses (N = 40, each) were placed between the respective parental and the F-1 progeny, although closer to the Africanized than to the Italian parentals (Mahalanobis square distance = 6.2 versus 12.1, respectively). Consequently, the most common crosses at the beginning of the Africanization process would have generated individuals more similar to Africanized than to Italian bees. This adds a genetic explanation for the rapid changes in the populational morphometric profile in recently colonized areas. Africanized alleles of wing venation pattern genes are apparently dominant and epistatic.

“What Happens When Pollinators Get Sick? Behavioral Impacts of Honeybee Viruses”

Like all organisms on the planet, honeybees (Apis mellifera) are susceptible to infection with a wide variety of viruses. These viruses may produce infections with no visible symptoms or may have devastating consequences on both the individual bee and the entire hive. Deformed Wing Virus, a member of the Iflavirus group of viruses, has an RNA genome and has had a particularly important impact on bee health. It can be spread between bees in a several ways – bees can infect each other during feeding or grooming activities, drones can pass the virus to the queen during mating and queens can lay infected eggs. The primary and most devastating way that these viruses are transmitted within and between hives involves a parasitic mite, an animal known ominously as Varroa destructor. The talk will discuss the effect that viruses have on the health and behavior of honeybees and will outline the collaborative research activities of Drs. Evans and Pizzorno over the last 7 years.

Sex-Specific Differences in Pathogen Susceptibility in Honey Bees (Apis mellifera)

Sex-related differences in susceptibility to pathogens are a common phenomenon in animals. In the eusocial Hymenoptera the two female castes, workers and queens, are diploid and males are haploid. The haploid susceptibility hypothesis predicts that haploid males are more susceptible to pathogen infections compared to females. Here we test this hypothesis using adult male (drone) and female (worker) honey bees (Apis mellifera), inoculated with the gut endoparasite Nosema ceranae and/or black queen cell virus (BQCV). These pathogens were chosen due to previously reported synergistic interactions between Nosema apis and BQCV. Our data do not support synergistic interactions between N. ceranae and BQCV and also suggest that BQCV has limited effect on both drone and worker health, regardless of the infection level. However, the data clearly show that, despite lower levels of N. ceranae spores in drones than in workers, Nosema-infected drones had both a higher mortality and a lower body mass than non-infected drones, across all treatment groups, while the mortality and body mass of worker bees were largely unaffected by N. ceranae infection, suggesting that drones are more susceptible to this pathogen than workers. In conclusion, the data reveal considerable sex-specific differences in pathogen susceptibility in honey bees and highlight the importance of ultimate measures for determining susceptibility, such as mortality and body quality, rather than mere infection levels

Precision Agriculture without borders: Practical issues and improvements in farmland coverage with aerial vehicles.

This work presents a solution for the aerial coverage of a field by using a fleet of aerial vehicles. The use of Unmanned Aerial Vehicles allows to obtain high resolution mosaics to be used in Precision Agriculture techniques. This report is focus on providing a solution for the full simultaneous coverage problem taking into account restrictions as the required spatial resolution and overlap while maintaining similar light conditions and safety operation of the drones. Results obtained from real field tests are finally reported

Rapid prototyping framework for visual control of autonomous micro aerial vehicles

Rapid prototyping environments can speed up the research of visual control algorithms. We have designed and implemented a software framework for fast prototyping of visual control algorithms for Micro Aerial Vehicles (MAV). We have applied a combination of a proxy-based network communication architecture and a custom Application Programming Interface. This allows multiple experimental configurations, like drone swarms or distributed processing of a drone’s video stream. Currently, the framework supports a low-cost MAV: the Parrot AR.Drone. Real tests have been performed on this platform and the results show comparatively low figures of the extra communication delay introduced by the framework, while adding new functionalities and flexibility to the selected drone. This implementation is open-source and can be downloaded from www.vision4uav.com/?q=VC4MAV-FW

Diseño de un sistema para el manejo a distancia de aeronaves construidas con arduino

El principal objetivo del proyecto es intentar reducir los costes de algunas de las operaciones de vigilancia llevadas a cabo por agencias o instituciones de seguridad. El proyecto consiste en diseñar y desarrollar un sistema informático que permita el manejo a distancia de un cuadricóptero a través de un ordenador, utilizando el teclado y visualizando las imágenes recibidas del módulo de la videocámara. Cada cuadricóptero estará compuesto de diferentes módulos y cada módulo tiene una funcionalidad característica. Se desarrollará un sistema de gestión de aeronaves para poder añadir nuevas unidades de cuadricópteros, así como un sistema de gestión de usuarios para administrar los usuarios en el sistema. Adicionalmente, se construirá un prototipo de cuadricóptero y se implementará su unidad controladora para poder realizar las pruebas del sistema desarrollado con ello. ---ABSTRACT---The aim of this project is to attempt to reduce the costs of some surveillance services offered by security agencies or institutions. The project consists in designing and developing a computer system to remotely control a drone or quad-copter through a computer, manipulating the drone through the keyboard and watching the images captured from the camera module. Each drone is built with one or more modules, and each module has its own functionality. Both new drones and new users can be added to the computer system through the drone management system and the user management system, respectively. Both of management systems are going to be developed. The project also includes the making of a quad-copter prototype and a controller unit implementation.

Modelos digitales del terreno mediante fotogrametría aérea realizada con un vehículo aéreo no tripulado

En las últimas décadas, el avance tecnológico ha aumentado a una velocidad vertiginosa. Muchos son los campos beneficiados, entre ellos la ingeniería y por consiguiente el desarrollo de maquinaria, técnicas y herramientas que facilitan el trabajo. Los vehículos aéreos no tripulados (UAVs), más conocidos como drones, se presentan como una alternativa muy interesante para llevar a cabo levantamientos topográficos mediante la técnica fotogramétrica. En este proyecto se desarrollan los Modelos Digitales del Terreno (MDT) de una superficie sobrevolada por un UAV en la localidad de Cubillos del Sil (León). Para ello, se han utilizado dos software: Mission Planner y PhotoModeler. En el primero se ha llevado a cabo la programación de la misión de vuelo del drone, siendo el fin la toma de fotografías aéreas. El segundo es el encargado de realizar la reconstrucción digital en 3D del terreno sobrevolado a partir de esas fotografías. El objetivo final es que los MDT obtenidos puedan ser utilizados en un futuro por cualquier equipo de trabajo que quiera desarrollar un proyecto sobre esos terrenos. ABSTRACT The pace of technology innovation is faster now than it has been for the past few decades. Engineering, and consequently machinery and diverse techniques, are the main beneficiaries. A clear example of this is the development of unmanned aerial vehicles (UAVs), more commonly known as drones. They are a very good alternative that can be used to carry out topographic surveys through photogrammetry. In this project the Digital Terrain Models (DTM) of an overflown area in the municipality of Cubillos del Sil (León) are developed. Two main pieces of software have been used for this purpose; Mission Planner and PhotoModeler. Programming of the drone flight mission was done with the former, allowing it to take aerial pictures, whilst the latter was used to digitally rebuild the overflown area in 3D, using the pictures from the first piece of software. The final aim of the project is to make the obtained DTM available for any future project.

Diseño de un cuadricóptero de bajo coste basado en herramientas de software libre

El presente proyecto tiene como objetivo la realización de un cuadricóptero de bajo coste desarrollado con herramientas de software libre, con el fin de permitir el acceso y modificación del mismo a cualquiera que posea los conocimientos necesarios. Los cuadricópteros se definen como un vehículo aéreo no tripulado con cuatro rotores en los extremos. Los ejemplos existentes de estos tipos de vehículos son casi siempre de grandes cuadricópteros, los cuales utilizan diferentes tecnologías en los motores y control de los mismos. Los cuadricópteros de similar tamaño al que se pretende realizar son por lo general de compañías con hardware y software cerrado. En el caso de este proyecto se ha realizado un cuadricóptero de tamaño menor a 5 x 5 cm. La innovación que se propone con este proyecto es una forma de detectar obstáculos para cuadricópteros de tamaño similar, en los que la limitación del peso del dron supone una disminución de las opciones disponibles. Se desea que el cuadricóptero sea capaz de realizar un vuelo estable detectando y esquivando obstáculos sin necesidad de ayuda externa como operadores con mando de radio frecuencia. Para la creación del cuadricóptero, se ha realizado tanto el diseño de los esquemáticos como el diseño de las huellas para la utilización en el desarrollo de la PCB. Para ello se ha hecho uso de herramientas de software libre como es Kicad, software para el desarrollo de esquemáticos y placas de circuito impreso con las funcionalidades principales de cualquier software privativo relacionado. Se pretende de esta forma aportar a la literatura, un aspecto práctico de la realización de cuadricópteros, tanto desde los aspectos teóricos del diseño como los aspectos prácticos de la fabricación y soldadura de los componentes del cuadricóptero. En la realización del presente proyecto se ha tenido en cuenta los diferentes algoritmos que existen para la fusión de datos de la unidad de medida inercial, tanto la facilidad de implementación de los mismos como la facilidad de los cálculos resultantes de esta implementación. Se ha hecho una implementación de un filtro complementario, dando resultados satisfactorios debido a las características intrínsecas de la unidad de medida inercial. Además del filtro complementario, se ha realizado una implementación del filtro diseñado por Sebastian Madgwick [1]. Este filtro está especialmente diseñado para la fusión de los datos provenientes de la unidad de medida inercial, proporcionando la orientación del sistema haciendo uso de la representación en cuaternios de los datos del acelerómetro y giróscopo, permitiendo el uso del método del gradiente para el cálculo del error del giróscopo. Para la selección de los componentes, se ha hecho un análisis pormenorizado de las diferentes opciones disponibles, tomando como punto de partida los cuadricópteros que existen en la actualidad. Se han elegido estos componentes en función de las características de los mismos, prestando especial atención al tamaño, relacionado directamente con el peso de los mismos así como del precio, para lograr un cuadricóptero fácilmente reproducible de bajo coste. En este análisis se ha tenido en cuenta las dificultades existentes en la selección de determinados componentes como son los motores y las hélices. Al ser estos dos componentes caracterizados mediante tablas creadas por los fabricantes y usuarios de los mismos, la selección de los mismos se ha visto dificultada a la hora de elegir componentes de coste reducido al poseer poca información sobre los mismos. En especial, las formulas desarrolladas para el cálculo del empuje de los motores están directamente relacionados con los parámetros de las hélices. Estos parámetros están caracterizados para la mayoría de las hélices comerciales utilizadas en cuadricópteros. Para caracterizar las hélices se utiliza un banco de trabajo en donde es posible medir el empuje realizado por el conjunto del motor y hélice. En el caso del presente proyecto, no se disponía de la herramienta necesaria por lo que se ha realizado una estimación de los parámetros en función de las tablas disponibles para hélices similares de mayor tamaño. Para la elección de los sensores para la detección de los obstáculos se ha realizado un estudio de los diferentes sensores disponibles, analizando las ventajas y desventajas de los mismos para la elección del más adecuado para el proyecto. Se ha decidido el uso de sensores de distancia basados en tecnología infrarroja por ser los únicos que se adaptan a los requisitos de peso impuesto por el modelo. Además en este proyecto se ha realizado el montaje y soldadura de los componentes de la PCB. Estos componentes al ser de tamaño reducido, se ha comprobado que para la soldadura de los mismos es necesario el uso de herramientas especializadas, como puede ser estaciones de soldadura y pistola de aire caliente lo que dificulta su soldadura de manera no profesional. Al término de este proyecto se ha comprobado la dificultad de la realización de una correcta soldadura de los componentes, lo que introduce errores de conectividad entre los componentes, en concreto se ha detectado errores entre el microprocesador y unidad de medida inercial. Además de estos errores, se ha comprobado la dificultad de regular el sistema, no logrando un vuelo estable en el tiempo de escritura del presente proyecto. Por último se presenta el prototipo creado a lo largo del proyecto, al cual se le pueden hacer diferentes modificaciones como posibles líneas futuras, entre las que se encuentran una mejor regulación que permita el vuelo de un conjunto de drones.

Estudio, ensamblaje, caracterización y ensayos de dos modelos reales de RPA

Un dron o un RPA (del inglés, Remote Piloted Aircraft) es un vehículo aéreo no tripulado capaz de despegar, volar y aterrizar de forma autónoma, semiautónoma o manual, siempre con control remoto. Además, toda aeronave de estas características debe ser capaz de mantener un nivel de vuelo controlado y sostenido. A lo largo de los años, estos aparatos han ido evolución tanto en aplicaciones como en su estética y características físicas, siempre impulsado por los requerimientos militares en cada momento. Gracias a este desarrollo, hoy en día los drones son uno más en la sociedad y desempeñan tareas que para cualquier ser humano serían peligrosas o difíciles de llevar a cabo. Debido a la reciente proliferación de los RPA, los gobiernos de los distintos países se han visto obligados a redactar nuevas leyes y/o modificar las ya existentes en relación a los diferentes usos del espacio aéreo para promover la convivencia de estas aeronaves con el resto de vehículos aéreos. El objeto principal de este proyecto es ensamblar, caracterizar y configurar dos modelos reales de dron: el DJI F450 y el TAROT t810. Para conseguir un montaje apropiado a las aplicaciones posteriores que se les va a dar, antes de su construcción se ha realizado un estudio individualizado en detalle de cada una de las partes y módulos que componen estos vehículos. Adicionalmente, se ha investigado acerca de los distintos tipos de sistemas de transmisión de control remoto, vídeo y telemetría, sin dejar de lado las baterías que impulsarán al aparato durante sus vuelos. De este modo, es sabido que los RPA están compuestos por distintos módulos operativos: los principales, todo aquel módulo para que el aparato pueda volar, y los complementarios, que son aquellos que dotan a cada aeronave de características adicionales y personalizadas que lo hacen apto para diferentes usos. A lo largo de este proyecto se han instalado y probado diferentes módulos adicionales en cada uno de los drones, además de estar ambos constituidos por distintos bloques principales, incluyendo el controlador principal: NAZA-M Lite instalado en el dron DJI F450 y NAZA-M V2 incorporado en el TAROT t810. De esta forma se ha podido establecer una comparativa real acerca del comportamiento de éstos, tanto de forma conjunta como de ambos controladores individualmente. Tras la evaluación experimental obtenida tras diversas pruebas de vuelo, se puede concluir que ambos modelos de controladores se ajustan a las necesidades demandadas por el proyecto y sus futuras aplicaciones, siendo más apropiada la elección del modelo M Lite por motivos estrictamente económicos, ya que su comportamiento en entornos recreativos es similar al modelo M V2. ABSTRACT. A drone or RPA (Remote Piloted Aircraft) is an unmanned aerial vehicle that is able to take off, to fly and to land autonomously, semi-autonomously or manually, always connected via remote control. In addition, these aircrafts must be able to keep a controlled and sustained flight level. Over the years, the applications for these devices have evolved as much as their aesthetics and physical features both boosted by the military needs along time. Thanks to this development, nowadays drones are part of our society, executing tasks potentially dangerous or difficult to complete by humans. Due to the recent proliferation of RPA, governments worldwide have been forced to draft legislation and/or modify the existing ones about the different uses of the aerial space to promote the cohabitation of these aircrafts with the rest of the aerial vehicles. The main objective of this project is to assemble, to characterize and to set-up two real drone models: DJI F450 and TAROT t810. Before constructing the vehicles, a detailed study of each part and module that composes them has been carried out, in order to get an appropriate structure for their expected uses. Additionally, the different kinds of remote control, video and telemetry transmission systems have been investigated, including the batteries that will power the aircrafts during their flights. RPA are made of several operative modules: main modules, i.e. those which make the aircraft fly, and complementary modules, that customize each aircraft and equip them with additional features, making them suitable for a particular use. Along this project, several complementary modules for each drone have been installed and tested. Furthermore, both are built from different main units, including the main controller: NAZA-M Lite installed on DJI F450 and NAZA-M V2 on board of TAROT t810. This way, it has been possible to establish an accurate comparison, related to the performance of both models, not only jointly but individually as well. After several flight tests and an experimental evaluation, it can be concluded that both main controller models are suitable for the requirements fixed for the project and the future applications, being more appropriate to choose the M Lite model strictly due to economic reasons, as its performance in recreational environment is similar to the M V2.

Common endocrine and genetic mechanisms of behavioral development in male and worker honey bees and the evolution of division of labor.

Temporal polyethism is a highly derived form of behavioral development displayed by social insects. Hormonal and genetic mechanisms regulating temporal polyethism in worker honey bees have been identified, but the evolution of these mechanisms is not well understood. We performed three experiments with male honey bees (drones) to investigate how mechanisms regulating temporal polyethism may have evolved because, relative to workers, drones display an intriguing combination of similarities and differences in behavioral development. We report that behavioral development in drones is regulated by mechanisms common to workers. In experiment 1, drones treated with the juvenile hormone (JH) analog methoprene started flying at significantly younger ages than did control drones, as is the case for workers. In experiment 2, there was an age-related increase in JH associated with the onset of drone flight, as in workers. In experiment 3, drones derived from workers with fast rates of behavioral development themselves started flying at younger ages than drones derived from workers with slower rates of behavioral development. These results suggest that endocrine and genetic mechanisms associated with temporal polyethism did not evolve strictly within the context of worker social behavior.

Human resource management, innovation and performance:looking across levels

The importance of innovation can hardly be exaggerated, given that landmark change has defined human progress in our technological age. The business pages of popular journals are replete with a dazzling array of inventions that have overturned existing ways of working and fundamentally changed human experience — from agricultural drones that offer farmers new ways to increase crop yield to genome editing that provides powerful insights into genetically baffling brain disorders. Innovation has become a topical theme within organisations, too, with no shortage of advice and suggestions often targeted at business leaders about how to craft an innovation strategy or increase the number and quality of ideas with a view to enriching organisational life. The quote at the start of this chapter bears testament to the sheer effort of moving away from familiar, habitual practices in the direction of less-certain, risky future terrain. Setting aside what has gone before to move in new directions requires determination, resilience and courage at a personal level. Often overlooked, though, are the multi-level dynamics that this entails.

Resonances: Noise and Contemporary Music

Noise seems to stand for a lack of aesthetic grace, to alienate or distract rather than enrapture. And yet the drones of psychedelia, the racket of garage rock and punk, the thudding of rave, the feedback of shoegaze and post-rock, the bombast of thrash and metal, the clatter of jungle and the stuttering of electronica, together with notable examples of avant-garde noise art, have all found a place in the history of contemporary musics, and are recognised as representing key evolutionary moments. Noise therefore is the untold story of contemporary popular music, and in a critical exploration of noise lies the possibility of a new narrative – one that is wide-ranging, connects the popular to the underground and avant-garde, fully posits the studio as a musical instrument, and demands new critical and theoretical paradigms of those seeking to write about music. Resonances is a compelling collection of new essays by scholars, writers and musicians – all seeking to explore and enlighten this field of study.