Production of cellulolytic and hemicellulolytic enzymes from Aureobasidium pulluans on solid state fermentation

This article investigates a strain of the yeast Aureobasidium pullulans for cellulase and hemicellulase production in solid state fermentation. Among the substrates analyzed, the wheat bran culture presented the highest enzymatic production (1.05 U/mL endoglucanase, 1.3 U/mL β-glucosidase, and 5.0 U/mL xylanase). Avicelase activity was not detected. The optimum pH and temperature for xylanase, endoglucanase and β-glucosidase were 5.0 and 50, 4.5 and 60, 4.0 and 75°C, respectively. These enzymes remained stable between a wide range of pH. The β-glucosidase was the most thermostable enzyme, remaining 100% active when incubated at 75°C for 1 h. © 2007 Humana Press Inc.

Ultra-structural mapping of sugarcane bagasse after oxalic acid fiber expansion (OAFEX) and ethanol production by Candida shehatae and Saccharomyces cerevisiae

Background: Diminishing supplies of fossil fuels and oil spills are rousing to explore the alternative sources of energy that can be produced from non-food/feed-based substrates. Due to its abundance, sugarcane bagasse (SB) could be a model substrate for the second-generation biofuel cellulosic ethanol. However, the efficient bioconversion of SB remains a challenge for the commercial production of cellulosic ethanol. We hypothesized that oxalic-acid-mediated thermochemical pretreatment (OAFEX) would overcome the native recalcitrance of SB by enhancing the cellulase amenability toward the embedded cellulosic microfibrils. Results: OAFEX treatment revealed the solubilization of hemicellulose releasing sugars (12.56 g/l xylose and 1.85 g/l glucose), leaving cellulignin in an accessible form for enzymatic hydrolysis. The highest hydrolytic efficiency (66.51%) of cellulignin was achieved by enzymatic hydrolysis (Celluclast 1.5 L and Novozym 188). The ultrastructure characterization of SB using scanning electron microscopy (SEM), atomic force microscopy (AFM), Raman spectroscopy, Fourier transform-near infrared spectroscopy (FT-NIR), Fourier transform infrared spectroscopy (FTIR), and X-ray diffraction (XRD) revealed structural differences before and after OAFEX treatment with enzymatic hydrolysis. Furthermore, fermentation mediated by C. shehatae UFMG HM52.2 and S. cerevisiae 174 showed fuel ethanol production from detoxified acid (3.2 g/l, yield 0.353 g/g; 0.52 g/l, yield, 0.246 g/g) and enzymatic hydrolysates (4.83 g/l, yield, 0.28 g/g; 6.6 g/l, yield 0.46 g/g). Conclusions: OAFEX treatment revealed marked hemicellulose degradation, improving the cellulases ability to access the cellulignin and release fermentable sugars from the pretreated substrate. The ultrastructure of SB after OAFEX and enzymatic hydrolysis of cellulignin established thorough insights at the molecular level. © 2013 Chandel et al; licensee BioMed Central Ltd.

The enzymatic recalcitrance of internodes of sugar cane hybrids with contrasting lignin contents

The recalcitrance in grasses varies according to cell type and maturation. The origin of the recalcitrance in different regions from sugar cane internodes with varied lignin contents was evaluated. The efficiency of enzymatic hydrolysis was correlated with the chemical, micromorphological and microspectrophotometric characteristics of the samples. The internodes of three sugar cane hybrids were dissected into four different fractions. The outermost fraction and the rind were the most recalcitrant regions, whereas the pith-rind interface and the pith were less recalcitrant. Cellulose conversion reached 86% after 72h of enzymatic digestion of the pith from the hybrid with the lowest lignin content. There was an inversely proportional correlation between the area occupied by vascular bundles and the efficiency of cellulose hydrolysis. High cellulose and low lignin or hemicellulose contents enhanced the efficiency of enzymatic hydrolysis of the polysaccharides. The critical evaluation of the results permitted to propose an empirical parameter for predicting cellulose conversion levels that accounts for the positive effect of high cellulose and low lignin plus hemicellulose and the detrimental effect of abundant vascular bundles. The cellulose conversion levels fit well to this calculated parameter, following a second order polynomial with an r2 value of 0.96. © 2013 Elsevier B.V.

Caracterização biofísica e estrutural da metaloproteinase não-hemorrágica do veneno de Bothrops moojeni e da endo-β-glicanase do Bacillus subtilis

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Prospecção de enzimas de degradação de materials vegetal em fungos endofíticos

Pós-graduação em Biotecnologia - IQ

Avaliação do uso de radiação micro-ondas e ultrassom combinados com agentes químicos como pré-tratamentos de bagaço de cana-de-açúcar para posterior sacarificação enzimática

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Produção de enzimas despolimerizantes de materal vegetal por fungos termofílicos usando suplemento comercial como substrato

Pós-graduação em Microbiologia - IBILCE

Comparação da produção de celulases e xilanases por fungos filamentosos em fermentação submersa e estado sólido

Pós-graduação em Microbiologia - IBILCE

Análise in silico de um novo “cluster” de PKS II Metagenômico

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Isolamento de fungos termofílicos produtores de celulases, xilanases e ferruloil esterase para bioconversão de bagaço de cana de açúcar em açúcares fermentescíveis

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Seleção de microrganismos celulolíticos, imobilização e aplicação das celulases, produzidas e comercial, na hidrólise da celulose

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Produção e avaliação de enzimas fibrolíticas exógenas na ensilagem de milho

Pós-graduação em Zootecnia - FCAV

Utilização de enzimas produzidas por Trichoderma reesei E Aspergillus niger na extração de óleos essenciais

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Dinâmica de carbono e nitrogênio em pastos de capim-xaraés submetidos a diferentes resíduos pós-pastejo

Pós-graduação em Zootecnia - FCAV

Identificação de colletotrichum gloeosporioides de atemóia (Annona cherimola x Annona squamosa), por meio de caracterização patogênica, cultural e morfológica

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)