Purificação e estudos bioquímicos de exo-poligalacturonase (pectinase) produzida pelo fungo termofílico Rhizomucor pusillus, em cultivo submerso e aplicação na extração de sucos de frutas e hidrólise enzimática do bagaço de cana-de-açúcar

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Avaliação térmica e estrutural do bagaço de cana de açúcar pré-tratado com ozônio, ultrassom e micro-ondas para produção de etanol celulósico por hidrólise enzimática

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Bioprospecção de leveduras fermentadoras de xilose visando a produção de etanol a partir de bagaço de cana-de-açúcar

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Preparação e caracterização de materiais híbridos celulose/NbOPO4.nH2O a partir de celulose branqueada de bagaço de cana-de-açúcar

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Pré-hidrólise ácida de bagaço de cana-de-açúcar e sua caracterização físico-química

The biomass resulting from processing sugarcane bagasse has been considered a source of cellulose with the potential production of bio-fuels. This lignocellulose can be processed into ethanol since is hydrolyzed by chemical processes (acids) or biotechnology (enzymes) which generate sugars suit for fermentation. This study had the objective to utilize physical and chemical pre-treatment processes for prehydrolysis of sugarcane bagasse. The experimental treatment was adjusted at a factor of 4 X 2, by the combination of pre-hydrolysis timing (15, 30, 45 and 60 minutes) and sulfuric acid concentrations (7.0% and 9.0%) which was incubated at a temperature of 121° C in an autoclave. The treatment data was subjected to analysis of the variance and averages which were compared using the Tukey test with a probability of 5%. The results obtained showed that through pretreatment acid applied on the lignocellulose material, there was a significant break from the substrate fibers like cellulose, hemicellulose and lignin.

Determinação do teor de glicose em bagaço de cana-de-açúcar hidrolizado pelo processo de conversão enzimática

The production of ethanol and sugar from sugarcane juice generate as byproduct, the bagasse. Currently, the bagasse, an industrial lignocellulosic biomass, can be used for production of second-generation ethanol, since when it is submitted to hydrolytic processes generates fermentable sugars. The objective of this study was to produce fungal enzymes capable of hydrolyzing this lignocellulosic biomass to generate glucose. For this, we used the mushroom species Lentinula edodes, Pleurotus ostreatus, Pleurotus eryngii, and Pycnoporus sanguineus as potential sources of laccase, manganese peroxidase and lignin peroxidase enzymes, capable of hydrolyzing the crushed sugarcane. The hydrolysis process was performed with the highest enzymatic activities observed from laccase in L. edodes (39.23 U-mL after 25 day incubation), P. ostreatus (2.5 U U-mL after 27 day incubation), P. sanguineus (80 U-mL after 27 days of incubation) and P. eryngii (16.45 U-mL 15 days incubation). MnP and LiP showed no significant results. The enzymatic hydrolysis of sugarcane bagasse in natura (32,17% hemicellulose, cellulose 52,45% and 10,62% lignin) and bagasse hydrolyzate with 7,0% H2SO4 (0,20% hemicellulose, 68,82% to 25,33% cellulose and lignin) were evaluated for each enzymatic obtained. Compared to others, the enzymes produced by P. sanguineus incubated in sugarcane bagasse showed better efficiency resulting in glucose with an average content of 0,14 g-L. Although the levels of glucose determined in this work were low in relation to the literature, it can be stated that the laccase, manganese peroxidase and lignin peroxidase enzymes demonstrated good hydrolytic potential, especially those produced by the fungus P. sanguineus.

Cultivo de Lentinula edodes e Pleurotus ostreatus em bagaço de cana-de-açúcar

Pós-graduação em Microbiologia - IBILCE

Efeito prebiótico de xilo-oligossacarídeos produzidos a partir da hemicelulose de bagaço de cana-de-açúcar utilizando extração alcalina e hidrólise de xilanases fúngicas

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Pré-tratamento do bagaço de cana-de-açúcar, hidrólise e sua caracterização química e estrutural, visando a produção de etanol de segunda geração

Pós-graduação em Biotecnologia - IQ

Métodos de extração da lignina do bagaço da cana-de-açúcar do Noroeste do Estado de São Paulo

Pós-graduação em Química - IBILCE

Estimativa do potencial de acréscimo da oferta de energia no setor sucroalcooleiro paulista a partir de cogeração e gaseificação do bagaço de cana-de-açúcar com utilização de ciclo combinado

This work evaluates the existing potential in the state of Sao Paulo for the generation of electrical energy using the sugar cane bagasse as fuel. As the bagasse is a by-product of the sugarcane and alcohol industry and it is produced in large scale in the country, mainly in the state of Sao Paulo, it is important to develop researches that aim the best utilization of this input. In order to determine its potential, at first, a study was conducted considering the utilization of the cogeneration, which is a common practice in the plants of the sector. However, it was concluded that the cogeneration could provide a higher quantity of energy if more modern technologies and more efficient processes were used. Another study to estimate the potential considered a system of gasification of the sugar cane bagasse integrated with the combined cycle (BIG/GTCC). It was concluded that this technology can provide a considerable increase in the electrical supply. In this work it was also developed an energetic study based on real data from a plant located in the state of Sao Paulo. A thermodynamic analysis was done in the existing equipment of the cogeneration section of the plant. And another analysis was done considering the implementation of the BIG/GTCC technology to the cogeneration system. Comparing the results of both settings, it was concluded that the utilization of the sugar cane bagasse integrated to a combined cycle increased considerably the efficiency in the generation of electricity of the plant, increasing more than six times its production capacity of electrical energy

Produção de enzimas lignocelulósicas por fermentação em estado sólido por espécimes do gênero Trichoderma para sacarificação do bagaço de cana de açúcar tratado com micro-ondas

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Métodos de extração da lignina do bagaço da cana-de-açúcar do Noroeste do Estado de São Paulo

Pós-graduação em Química - IBILCE

Estimativa do potencial de acréscimo da oferta de energia no setor sucroalcooleiro paulista a partir de cogeração e gaseificação do bagaço de cana-de-açúcar com utilização de ciclo combinado

This work evaluates the existing potential in the state of Sao Paulo for the generation of electrical energy using the sugar cane bagasse as fuel. As the bagasse is a by-product of the sugarcane and alcohol industry and it is produced in large scale in the country, mainly in the state of Sao Paulo, it is important to develop researches that aim the best utilization of this input. In order to determine its potential, at first, a study was conducted considering the utilization of the cogeneration, which is a common practice in the plants of the sector. However, it was concluded that the cogeneration could provide a higher quantity of energy if more modern technologies and more efficient processes were used. Another study to estimate the potential considered a system of gasification of the sugar cane bagasse integrated with the combined cycle (BIG/GTCC). It was concluded that this technology can provide a considerable increase in the electrical supply. In this work it was also developed an energetic study based on real data from a plant located in the state of Sao Paulo. A thermodynamic analysis was done in the existing equipment of the cogeneration section of the plant. And another analysis was done considering the implementation of the BIG/GTCC technology to the cogeneration system. Comparing the results of both settings, it was concluded that the utilization of the sugar cane bagasse integrated to a combined cycle increased considerably the efficiency in the generation of electricity of the plant, increasing more than six times its production capacity of electrical energy

Produção de enzimas lignocelulósicas por fermentação em estado sólido por espécimes do gênero Trichoderma para sacarificação do bagaço de cana de açúcar tratado com micro-ondas

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)