The Acropolitan - v. 1, no. 1

In this issue...Charlie McAuliffe, ore-diggers, Rhode's Scholarship, Glee Club, homecoming, George Fowler, Clark Park, Alumni banquet

The Acropolitan - v. 1, no. 3

In this issue...First aid, Anaconda Copper Mining Company, Butte Y.M.C.A., School of Mines gymnasium, Oratory contest, Glee Club

The Acropolitan - v. 1, no. 4

In this issue...Main Hall, Coach McAuliffe, State Bureau of Mines, Butte, Montana, Emmet Cullity, smelter, zinc, Moulton mine

The Acropolitan - v. 1, no. 6

In this issue...commencement, Butte, Montana, W. B. Daly, Anaconda Copper Mining Company, Masonic Hall, white-washing the M, Rocky Mountain Garden Club

The Acropolitan - v. 1, no. 5

In this issue...Big Butte, M Days, Thornton Hotel, Butte, Montana, Hennessy's, Anaconda Copper Mining Geological Department, Gamer's Cafe

The Amplifier - v. 1, no. 1

In this issue...Dr. Van Pelt, Blaine School, Anderson Carlisle Society, Mineral Club, Newman Club, Father Garrity, Sigma RHO, Theta TAU, Arnold Air Society

The Amplifier - v. 1, no. 3

In this issue...M-Day, Convocation, Student Council, Copper Guards, Gary Mitton, Clark Park, Butte Rotary Club, Tom Davis Memorial, blood donations

The Amplifier - v. 1, no. 2

In this issue...Baker Oil Tool Co., Golf Team, Coed Club, Student Wives, Butte High Band, AFROTC, Whiskey Gulch, M-Club, Montana State University, Debate Team

The Amplifier - v. 1, no. 4

In this issue...Sigma RHO, M-Days, Marcus Daly, Moonshiner's Ball, Petroleum Engineering, Naval Air Corps, Loretta Buss Peck, Mines football team

The Amplifier - v. 1, no. 5

In this issue...Montana Power, Debate Team, Anderson Carlisle Society, Carroll College, Anaconda Copper Mining Company, Copper Guards, Moonshiners Ball

The Amplifier - v. 1, no. 6

In this issue...Lydias, Jack Hunter, U. S. Navy, Student Council, Drama class, Petroleum Engineering, G. I. Bill, Air Force ROTC, General Guthrie, Coach Olsen

The Amplifier - v. 1, no. 7

In this issue...Dr. Carlson, Liberal Arts, Peggie Stobie, M Club, Charlie Russell, St. Patrick's Church, New York Times, Drama Club, Technical Writing, George Cloudy

The Amplifier - v. 1, no. 8

In this issue...Socony Exploration Company, Mobil Producing Company, Commencement, George Mealey, honorary degree, Butte, Montana

The Amplifier - v. 1, no. 9

In this issue...Dr. Van Pelt, Lydia's, Old Faithful, Yellowstone National Park, U. S. Gypsum Plant, Stanolind Oil & Gas Company, Coach Olsen, Great Falls Brewery

A novel rare variant in SCN1Bb linked to Brugada syndrome and SIDS by combined modulation of Na(v)1.5 and K(v)4.3 channel currents.

BACKGROUND Cardiac sodium channel β-subunit mutations have been associated with several inherited cardiac arrhythmia syndromes. OBJECTIVE To identify and characterize variations in SCN1Bb associated with Brugada syndrome (BrS) and sudden infant death syndrome (SIDS). METHODS All known exons and intron borders of the BrS-susceptibility genes were amplified and sequenced in both directions. Wild type (WT) and mutant genes were expressed in TSA201 cells and studied using co-immunoprecipitation and whole-cell patch-clamp techniques. RESULTS Patient 1 was a 44-year-old man with an ajmaline-induced type 1 ST-segment elevation in V1 and V2 supporting the diagnosis of BrS. Patient 2 was a 62-year-old woman displaying a coved-type BrS electrocardiogram who developed cardiac arrest during fever. Patient 3 was a 4-month-old female SIDS case. A R214Q variant was detected in exon 3A of SCN1Bb (Na(v)1B) in all three probands, but not in any other gene previously associated with BrS or SIDS. R214Q was identified in 4 of 807 ethnically-matched healthy controls (0.50%). Co-expression of SCN5A/WT + SCN1Bb/R214Q resulted in peak sodium channel current (I(Na)) 56.5% smaller compared to SCN5A/WT + SCN1Bb/WT (n = 11-12, P<0.05). Co-expression of KCND3/WT + SCN1Bb/R214Q induced a Kv4.3 current (transient outward potassium current, I(to)) 70.6% greater compared with KCND3/WT + SCN1Bb/WT (n = 10-11, P<0.01). Co-immunoprecipitation indicated structural association between Na(v)β1B and Na(v)1.5 and K(v)4.3. CONCLUSION Our results suggest that R214Q variation in SCN1Bb is a functional polymorphism that may serve as a modifier of the substrate responsible for BrS or SIDS phenotypes via a combined loss of function of sodium channel current and gain of function of transient outward potassium current.