Atividade biológica de derivados vegetais de famílias da flora brasileira sobre as linhagens I e II de Trypanosoma cruzi (Kinetoplastida, Trypanosomatidae)

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Avaliação eletrofisiológica e histopatológica do músculo estriado esquelético em cães naturalmente acometidos pela leishmaniose visceral

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Síntese, caracterização e estudo de atividade biológica de complexos de prata(I) contendo ligantes baseados na 1,10-fenantrolina e tiouréia

Pós-graduação em Química - IQ

In Vitro Antiparasitic Activity and Chemical Composition of the Essential Oil Obtained from the Fruits of Piper cubeba

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Leishmanicidal Activities of Novel Synthetic Furoxan and Benzofuroxan Derivatives

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Morphological changes and parasite load of the adrenal from dogs with visceral leishmaniasis

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Comparison of PCR with stained slides of bone marrow and lymph nodes aspirates with suspect diagnosis for leishmaniasis

Visceral leishmaniasis (VL), also known as kala-azar, is a disseminated protozoan infection caused by Leishmania donovani complex. Traditionally the definite diagnosis is made by amastigote detection in the tissue. The aim this study was to evaluate the PCR technique in stained slides of bone marrow and lymph nodes aspirates with suspect diagnosis for leishmaniasis. Slides were selected totaling 62 suspect cases (33 bone marrow samples and 29 lymph node samples) and 17 positive cases (8 bone marrow and 9 lymph node). From 62 suspect cases, 39 (62.90%) were confirmed to be positive being 17 (n = 29) lymph node aspirates and 22 (n = 33) bone marrow. This finding is in agreement with the higher sensitivity of the PCR assay compared to direct microscopic observation. In conclusion, the findings of this study supports the use of PCR on archive cytological preparation stained slides for the diagnosis of canine visceral leishmaniasis, emphasizing the higher sensitivity of this technique when compared to direct microscopic examination and mostly the use of the suspect status for the cytology samples that presents the previously mentioned particularities with focus on detecting the oligosymptomatic or assymptomatic dogs in endemic areas functioning as potential reservoirs for this disease. (C) 2014 Elsevier B.V. All rights reserved.

Canine osteomyelitis caused by Leishmania sp

Leishmaniasis is a disease emerging or re-emerging worldwide distribution (Sousa, 2008), a major impact on public health. The canine visceral leishmaniasis is an infectious parasitic zoonosis of worldwide distribution (Troncarelli, 2009), caused by a flagellate protozoan called Leishmania chagasi (Costard, 2009). Osteomyelitis can develop when the parasite reaches the bone tissue of the host via blood or continuity of adjacent soft tissue infection (Baltenperger, 2004). The histopathology of the lesions has 100% specificity when viewing the amastigote form of the parasite (Feitosa et al., 2000). A dog breed poodle, female, five years, with chronic lameness four months ago was attended by a veterinary, proceeded to the general clinical examination, radiographic evaluation of the hindquarters and the laboratory tests of enzyme immunoassay (ELISA) and indirect immunofluorescence (RIFI) for Leishmania sp. On examination, besides the enlargement of lymph nodes, the animal showed pain on flexion and extension of hind limbs. In radiographic lytic bone lesions were observed in bilateral ischial board and greater trochanter of the femur, suggestive of osteomyelitis. In specific laboratory tests for diagnosis of leishmaniasis ELISA reagent and RIFI reagent 1:40. As recommended by the Ministry of Health, the animal was euthanized. No macroscopic findings were reported during the necropsy, usually associated with leishmaniasis. The ischium bone fragments were sent for histopathological examination. There was intense proliferation of mononuclear inflammatory cells, mainly macrophages and lymphocytes. Amastigotes of Leishmania sp, were identified in the cytoplasm of some macrophages and bone tissue. In endemic areas for canine leishmaniasis, dogs with a history of intermittent lameness, and radiographic lytic bone lesions suggestive of osteomyelitis should be directed to realization of the histopathology and serologic tests for the differential diagnosis of Leishmania sp. Even without evidence of cutaneous or visceral lesions, usually associated with this disease.

Manifestações oculares na leishmaniose

The Canine Visceral Leishmaniasis is a chronic disease of endemic character, caused by Leishmania Chagasi in Americas. The inoculation of the promastigote form in the individual triggers a local and widespread immune reaction with formation of inflammatory infiltrates and deposition of immune complexes in tissues. Initial clinical symptoms of the disease are: weight reduction, hepatomegaly, splenomegaly and, according to the disease chronicity, signs such as alopecia, erythema, onychogryphosis, arthropathies, renal diseases, pyoderma, seborrheic dermatitis, muscle atrophy and Ocular diseases. Ocular diseases are often reported and are result of the direct parasitism or immune-mediated mechanisms caused by the disease. The Leishmania spp have greater affinity for the anterior segment, so that anterior uveitis is one of the most frequently diagnosed injuries. Blepharitis diffuse and Keratoconjunctivitis also appear as important ocular changes. In histological section, inflammatory infiltrates and macrophages with amastigote form are observed in all ocular tissues, with the exception of the retina and optical nerve. In the clinical analysis and disease diagnosis, should be considered the differential diagnosis, such as Ehrlichiosis and systemic hypertension, because these may cause some ocular manifestations similar to those observed in leishmaniasis

Detecção de marcadores de resistência a múltiplas drogas na pele de cães com infecção natural por Leishmania (Leishmania) infantum chagasi (Cunha e Chagas, 1937) Shaw, 2002, submetidos a diferentes protocolos de tratamento

Pós-graduação em Medicina Veterinária - FCAV

Síntese e avaliação biológica de bioisósteros de nitrofural ativos contra Leishmania amazonensis

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Infecção de Lutzomyia longipalpis por leishmania spp. em área urbana endêmica para leishmaniose visceral no Estado de São Paulo, Brasil

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Trypanosoma cruzi and Its soluble antigens induce NET release by stimulating toll-like receptors

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Morphological, immunohistochemical and molecular study of renal lesions in canine visceral leishmaniasis (CVL)

Canine visceral leishmaniasis (CVL) is an anthropozoonosis characterized by a clinically chronic progressive disease. Non lymphoid organs are also affected, especially the kidneys. Dogs with leishmaniasis usually die with renal failure despite treatment. Haematoxylin-eosin (HE) staining in kidney tissue sections has low sensitivity for parasite identification. Immunohistochemistry (IHC) and polymerase chain reaction (PCR) are efficient methods for Leishmania sp. antigen and DNA detection in cases of low parasite burden. The present study aims to identify renal lesions of CVL and correlate them with microscopic findings determined by histochemistry, IHC and PCR. Both IHC and PCR provided similar positivity for amastigote identification, 3/20 animals (15%), thus increasing detection of the parasite in renal tissues when compared with histopathologic examination. The lesion most commonly observed with visceral leishmaniasis-positive canine kidney tissue was membranoproliferative glomerulonephritis, followed by interstitial nephritis without correlation to the number of amastigotes.

Increase of leishmanicidal and tubercular activities using steroids linked to aminoquinoline

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)