Food safety in wine: optimization of analytical controls and evaluation of production technologies

This PhD thesis has been proposed to validate and then apply innovative analytical methodologies for the determination of compounds with harmful impact on human health, such as biogenic amines and ochratoxin A in wines. Therefore, the influence of production technology (pH, amino acids precursor and use of different malolactic starters) on biogenic amines content in wines was evaluated. An HPLC method for simultaneous determination of amino acids and amines with precolumnderivatization with 9-Fluorenyl-methoxycarbonyl chloride (FMOC-Cl) and UV detection was developed. Initially, the influence of pH, time of derivatization, gradient profile were studied. In order to improve the separation of amino acids and amines and reduce the time of analysis, it was decided to study the influence of different flows and the use of different columns in the chromatographic method. Firstly, a C18 Luna column was used and later two monolithic columns Chromolith in series. It appeared to be suitable for an easy, precise and accurate determination of a relatively large number of amino acids and amines in wines. This method was then applied on different wines produced in the Emilia Romagna region. The investigation permitted to discriminate between red and white wines. Amino acids content is related to the winemaking process. Biogenic amines content in these wines does not represent a possible toxicological problem for human health. The results of the study of influence of technologies and wine composition demonstrated that pH of wines and amino acids content are the most important factors. Particularly wines with pH > 3,5 show higher concentration of biogenic amines than wines with lower pH. The enrichment of wines by nutrients also influences the content of some biogenic amines that are higher in wines added with amino acids precursors. In this study, amino acids and biogenic amines are not statistically affected by strain of lactic acid bacteria inoculated as a starter for malolactic fermentation. An evaluation of different clean-up (SPE-MycoSep; IACs and LLE) and determination methods (HPLC and ELISA) of ochratoxin A was carried out. The results obtained proved that the SPE clean-up are reliable at the same level while the LLE procedures shows lowest recovery. The ELISA method gave a lower determination and a low reproducibility than HPLC method.

Studio sul convertitore elettronico per il pilotaggio di motori asincroni trifase

La semplicità, affidabilità, robustezza ed economicità del motore asincrono trifase ne fanno un motore largamente utilizzato sia in ambiente industriale che civile (pompe, ascensori, montacarichi, nastri trasportatori, ventilatori, locomotive ferroviarie, …). È ipotizzabile che in futuro vi sarà sempre più largo impiego di motori elettrici e i recenti sviluppi nella tecnologia dei convertitori elettronici per il controllo dei motori asincroni ne consentiranno l’uso in sempre più estesi ambiti applicativi. La messa sotto tensione di motori a media/alta potenza presenta però, in fase di avviamento, correnti di spunto e coppie elevate che devono essere controllate. In questa tesi saranno pertanto studiate le tecniche di protezione, avviamento e controllo dei motori asincroni trifase Nel primo capitolo esamineremo le funzioni di sezionamento, comando e protezione da cortocircuiti e sovraccarichi e i dispositivi adatti a svolgere tale compito in modo coordinato. Nel secondo capitolo esamineremo le principali tecnologie usate per l’avviamento dei motori, da quelle più semplici a quelle che impiegano dispositivi elettronici allo stato solido: Soft-Starters a tiristori (solo regolazione AC), Inverters a transistori (controllo sia in frequenza che ampiezza della forma d’onda AC generata). Fra tutti i possibili convertitori DC/AC analizzeremo dettagliatamente esclusivamente quelli a controllo PWM analogico che costituiranno l’oggetto della trattazione dei capitoli seguenti.

Heterogeneity in vaccination coverage explains the size and occurrence of measles epidemics in German surveillance data

The objective of this study was to characterize empirically the association between vaccination coverage and the size and occurrence of measles epidemics in Germany. In order to achieve this we analysed data routinely collected by the Robert Koch Institute, which comprise the weekly number of reported measles cases at all ages as well as estimates of vaccination coverage at the average age of entry into the school system. Coverage levels within each federal state of Germany are incorporated into a multivariate time-series model for infectious disease counts, which captures occasional outbreaks by means of an autoregressive component. The observed incidence pattern of measles for all ages is best described by using the log proportion of unvaccinated school starters in the autoregressive component of the model.

Is breast cancer risk the same for all progestogens?

The population-based case–control study CECILE investigated the impact of various menopausal hormone therapy (MHT) products on breast cancer (BC) risk in 1,555 postmenopausal women [1]. The case group (n = 739) included incident cases of in situ (!) or invasive BC in postmenopausal women. The control group (n = 816) included women from the general population within predefined quotas by age and socio-economic status (SES). While quotas by age were applied to obtain similar distributions by age among controls and among cases, quotas by SES in control women were applied to reflect the distribution by SES of women in the general population in the study area. Data of participants were obtained by a structured questionnaire during in-person interviews, and from pathology reports if applicable, respectively. Women were divided into current and past MHT user. MHTs were classified in estrogen-only therapy (ET), estrogen combined with progestin therapy (EPT) and tibolone. EPT was subdivided in three subtypes according to the progestogen constituent: natural micronized progesterone, progesterone derivatives, and testosterone derivatives. In comparison to never MHT users, any current or past MHT use (ET, EPT, tibolone) was not associated with an increased BC risk. However, in subanalysis BC risk was significantly increased for current use of EPT for 4 or more years (n = 73 cases and n = 56 controls, adjusted OR 1.55; 95 % CI 1.02–2.36). Within the group of current EPT users for 4 or more years, 14 cases had used estrogens combined with micronized progesterone (n = 17 controls), and 55 a combination with a synthetic progestogen (n = 34 controls), respectively. Compared to never MHT use, current use of EPT containing a synthetic progestogen for 4 or more years was associated with a significantly increased BC risk (adjusted OR 2.07; 95 % CI 1.26–3.39), but EPT containing micronized progesterone was not (adjusted OR 0.79; 95 % CI 0.37–1.71). 73 % of current MHT users started treatment within the first year of onset of menopause. Early EPT (n = 52 cases and n = 38 controls, adjusted OR 1.65; 95 % CI 1.02–2.69), but not early ET, starters had a significantly higher BC risk compared to never MHT users. In contrast, MHT initiation beyond 1 year after menopause was not associated with an increased BC risk. The authors concluded that: (1) ET and EPT containing natural progesterone did not increase BC risk whereas, (2) BC risk was increased in users of tibolone or EPT containing a synthetic progestogen, respectively, and that (3) MHT use early after onset of menopause was associated with an increased BC risk as compared to women who delay MHT beyond 1 or more years.

Virtualización de laboratorios

Para empezar, se ha hecho un análisis de las diferentes posibilidades que se podían implementar para poder conseguir el objetivo del trabajo. El resultado final debe ser, disponer de máquinas para que el sistema operativo fuese independiente del hardware que se tiene instalado en él . Para ello, se decide montar un sistema operativo de base en todos los equipos del laboratorio, que tenga las necesidades mínimas que se necesitan, las cuales son una interfaz gráfica y conexión de red. Hay que intentar reducir el consumo de recursos al máximo con este sistema operativo mínimo para que el rendimiento de las máquinas sea lo más fluido posible para los usuarios. El sistema elegido fue Linux con su distribución Ubuntu [ubu, http] con los módulos mínimos que permita funcionar el software necesario. Una vez se instala el sistema operativo anfitrión, se instala el escritorio Xfce [ubu2, http], que es el más ligero de Ubuntu, pero que proporciona buen rendimiento. Después, se procedió a instalar un software de virtualización en cada equipo. En este caso se decidió, por las buenas prestaciones que ofrecía, que fuera VirtualBox [vir2,http] de Oracle. Sobre éste software se crean tantas máquinas virtuales (con sistema operativo Windows) como asignaturas diferentes se cursan en el laboratorio donde se trabaje. Con esto, se consigue que al arrancar el programa los alumnos pudieran escoger qué máquina arrancar y lo que es más importante, se permite realizar cualquier cambio en el hardware (exceptuando el disco duro porque borraría todo lo que se tuviera guardado). Además de no tener que volver a reinstalar el sistema operativo nuevamente, se consigue la abstracción del software y hardware. También se decide que, para tener un respaldo de las máquinas virtuales que se tengan creadas en VirtualBox, se utiliza un servidor NAS. Uno de los motivos de utilizar dicho servidor fue por aprovechar una infraestructura ya creada. Un servidor NAS da la posibilidad de recuperar cualquier archivo (máquina virtual) cuando haga falta porque haya alguna máquina virtual corrupta en algún equipo, o en varios. Este tipo de servidor tiene la gran ventaja de ser multicast, es decir, permite solicitudes simultáneas. ABSTRACT For starters, there has been an analysis of the different possibilities that could be implemented to achieve the objective of the work. This objective was to have machines for the operating system to be independent of the hardware we have installed on it. Therefore, we decided to create an operating system based on all computers in the laboratory, taking the minimum needs we need. This is a graphical interface and network connection. We must try to reduce the consumption of resources to the maximum for the performance of the machines is as fluid as possible for users. The system was chosen with its Ubuntu Linux distribution with minimum modules that allow us to run software that is necessary for us. Once the base is installed, we install the Xfce desktop, which is the lightest of Ubuntu, but which provided good performance. Then we proceeded to install a virtualization software on each computer. In this case we decided, for good performance that gave us, it was Oracle VirtualBox. About this software create many virtual machines (Windows operating system) as different subjects are studied in the laboratory where we are. With that, we got it at program startup students could choose which machine start and what is more important, allowed us to make any changes to the hardware (except the hard drive because it would erase all we have). Besides not having to reinstall the operating system again, we get the software and hardware abstraction. We also decided that in order to have a backup of our virtual machines that we created in VirtualBox, we use a NAS server. One reason to use that server was to leverage their existing network infrastructure. A NAS server gives us the ability to retrieve any file (image) when we do need because there is some corrupt virtual machine in a team, or several. This is possible because this type of server allows multicast connection.

Hawkins electrical guide. Questions, answers & illustrations; a progressive course of study for engineers, electricians, students and those desiring to acquire a working knowledge of electricity and its applications; a practical treatise,

"Credit is largely due to Frank D. Graham ... for the authorship of the Guides, and for the original sketches illustrating electrical principles and construction."--Pref. to no. 1.

Ordnance field and depot maintenance : starter, engine, assembly 2920-753-8988, 2920-141-9508, and 2920-620-6821 (Jack & Heintz models D30, D42, and 20074-000).

"August 1959."

Mixed partitions of sets of triples into small planes

We continue our study of partitions of the set of all ((v)(3)) triples chosen from a v-set into pairwise disjoint planes with three points per line. We develop further necessary conditions for the existence of partitions of such sets into copies of PG(2, 2) and copies of AG(2, 3), and deal with the cases v = 13, 14, 15 and 17. These partitions, together with those already known for v = 12, 16 and 18, then become starters for recursive constructions of further infinite families of partitions. (C) 2004 Elsevier B.V. All rights reserved.

The Didache and Traditioned Innovation: Shaping Christian Community in the First Century and the Twenty-First Century

Church leaders, both lay and clergy, shape Christian community. Among their central tasks are: building communal identity, nurturing Christian practices, and developing faithful structures. When it comes to understanding the approach of the earliest Christian communities to these tasks, the Didache might well be the most important text most twenty-first century church leaders have never read. The Didache innovated on tradition, shaping the second generation of Christians to meet the crises and challenges of a changing world.

Most likely composed in the second half of the first century, the Didache served as a training manual for gentile converts to Christianity, preparing them for life in Christian community. This brief document, roughly one third the length of Mark’s gospel, developed within early Jewish-Christian communities. It soon found wide usage throughout the Mediterranean region, and its influence endured throughout the patristic and into the medieval period.

The Didache outlines emerging Christian practices that were rooted in both Jewish tradition and early Jesus material, yet were reaching forward in innovative ways. The Didache adopts historical teachings and practices and then adapts them for an evolving context. In this respect, the writers of the Didache, as well as the community shaped by its message, exemplify the pattern of thinking described by Greg Jones as “traditioned innovation.”

The Didache invites reflection on the shape and content of Christian community and Christian leadership in the twenty-first century. As churches and church leaders engage a rapidly changing world, the Didache is an unlikely and yet important conversation partner from two millennia ago. A quick read through its pages – a task accomplished in less than half an hour – brings the reader face to face with a brand of Christianity both very familiar and strikingly dissimilar to modern Christianity. Such dissonance challenges current assumptions about the church and creates a space in which to re-imagine our situation in light of this ancient Christian tradition. The Didache provides a window through which we might re-examine current conceptualizations of Christian life, liturgy, and leadership.

This thesis begins with an exploration of the form and function of the Didache and an examination of a number of important background issues for the informed study of the Didache. The central chapters of this thesis exegete and explore select passages in each of the three primary sections of the Didache – the Two Ways (Didache 1-6), the liturgical section (Didache 7-10), and the church order (Didache 11-15). In each instance, the composers of the Didache reach back into a cherished and life-giving aspect of the community’s heritage and shape it anew into a fresh and faithful approach to living the Christian life in a drastically different context.

The thesis concludes with three suggestions of how the Didache may provide a resource for the way the Church in the present thinks about training disciples, shaping community, and developing leadership structures. These conversation starters offer beginning points for a richer, fuller discussion of traditioned innovation in our current church context. The Didache provides a source of wisdom from our spiritual forebears that modern Christian leaders would do well not to ignore. With a look through the first century window of the Didache, twenty-first century Christians can discover fresh insights for shaping Christian community in the present.

What's in your Indian Takeaway?

Eating food prepared outside of the home has become the norm for adults on the island of Ireland. On 24% of eating or drinking occasions in the Republic of Ireland, food is cooked and prepared "out of the home". Bought-in cooked food makes up an average of 11% of a person’s energy intake in the United Kingdom. "Convenient" and "fast" cheap food has also become increasingly available to people. However, food prepared outside of the home has been found to contain considerably more dietary fat and less fibre and micronutrients than food prepared within the home. Traditionally, Indian diets are low in fat and high in fibre and rich in fruit and vegetables. However, these traditional meals have been adapted to suit Western palates and, as such, different ingredients in various quantities are added, potentially increasing their fat and salt content. Given the diversity of Indian takeaway dishes available on the island of Ireland, this survey has been designed to provide an insight into the energy (calorie), total fat, saturated fat, protein and salt content of the most popular starters, main courses and side dishes from various Indian takeaways and Indian restaurants with a takeaway service, as well as supermarkets or shop-bought equivalents. With two out of every three adults on the island of Ireland currently classified as overweight or obese, excess body weight is now one of the most important nutritional issues of our times. In addition to this, salt and fat intake is high and consumption of fruit, vegetables and fibre on the island of Ireland is low, increasing the risk of common chronic diseases, such as cardiovascular disease, diabetes and certain cancers. The consumption of excess calories and nutrient-poor foods contributes to our current obesity epidemic.  

Chromatographic determination of fatty acids profiles in regionally-produced Portuguese goat cheese along curing

The biochemistry of cheese ripening involves mechanisms such as glycolysis, proteolysis and lipolysis. Fatty acids are released by the action of lipases from different sources, milk, rennet, bacteria, moulds included as secondary starters, and other exogenous lipases, during lipolysis [1]. The composition of the lipid fraction contributes positively to the flavour of cheese, for being precursors of more complex aroma compounds responsible for the characteristic “goaty flavour” of goat cheeses [2]. Goat milk is recognized by its easier digestibility, alkalinity, buffering capacity and certain therapeutic values in medicine and human nutrition [3]. A high total content of fatty acids is strongly linked to a rancid and tart off flavour in goat milk and may be considered undesirable in most cheese varieties [4]. In this sense, the purpose of the present study was to examine the composition and changes in fatty acids and saponification value of goat cheese during curing period (2, 7 and 12 months). Goat cheese was made in industrial unit of Cachão - Mirandela (Trás-os- Montes) with raw milk Serrana goats’ race, salt and rennet from animal origin. During the first two months, the samples were stored in a ripening chamber (9.5-11 °C and RH 75-85%). From the second month to one year, the samples were stored in a preservation chamber (10.5-12 °C and RH 75-85%). The fatty acids profile of the inner part of the cheese was analyzed by gas-chromatography coupled to flame ionization detection (GC-FID). The degree of saponification was determined both in the crust and inside the cheese by HCl titration of ethanol KOH solution of the samples. Twenty-six fatty acids (FA) were identified and quantified in the inner part of the cheese (total fat was 45-46 g/100 g during the curing period). Saturated fatty acids (SFA) did not change up to 7 months of curing, increasing only after 12 months, being palmitic (C16:0), stearic (C18:0), myristic (C14:0) and capric (C10:0) acids the most abundant FA in this class. Monounsaturated fatty acids (MUFA) decreased only after 12 months, and oleic acid (C18:1) was the predominant FA. In polyunsaturated fatty acids (PUFA) class, the most abundant were linoleic (C18:2) and linolenic (C18:3) acids, and followed the same tendency of MUFA. This is corroborated by an increase in the degree of saponification, either in the crust as in the inner part of the cheese, after 12 months of curing, probably related with the saturation of the fatty acids [3]. Extra-long curing can be done in cheeses produced with goat milk up to seven months of storage without changing the total fat and individual FA content.

Estrategia del Grupo Volkswagen de México para la reducción del impacto ambiental generado por sus procesos productivos

El siguiente escrito pretende mostrar las estrategias que el Grupo Volkswagen de México implementó para reducir el impacto que generan sus dos fábricas en México al medio ambiente. La primera de ellas, y la más grande en cuanto a infraestructura, recurso humano, complejidad y productividad entre ambas, se ubica en el Estado de Puebla, en su capital Puebla. Se dedica a la fabricación de varios modelos de vehículos para el mercado interno y mundial. La segunda fábrica se ubica en la ciudad de Silao en el Estado de Guanajuato, esta fábrica se dedica principalmente a la fabricación de motores, los cuales posteriormente son distribuidos a distintos complejos productivos alrededor del mundo para allí ensamblarse en carrocerías. En primera instancia se describe la estrategia general con los debidos alcances establecidos desde la dirección de la empresa. Posteriormente se prosigue a desglosar cada una de las sub-estrategias, explicándolas y relacionándolas en contexto, tomando de esta manera, paso a paso, como se ha construido una campaña que ha logrado adquirir una identidad propia ampliamente reconocida tanto por la fuerza laboral de la compañía e inclusive por los agentes del mercado en el que se encuentran. Finalmente se reúnen datos numéricos relevantes en cuanto a la reducción del impacto ambiental que por último se ven reflejado en un ahorro económico para la empresa.

Estrategia y plan de mercadeo de la empresa YouTube

Esta investigación describe la situación de cómo Youtube se ha convertido a partir de sus estrategias y plan de mercadeo en la plataforma número uno en variedad de clips de películas, vídeos musicales, video de blogs, entre otros; llegando a popularizarse como una red social. Las redes sociales han desarrollado una nueva forma de comunicar y son una herramienta fundamental para la creación de conocimiento colectivo, es el caso de YouTube buscador de contenido audiovisual y red social que permite a millones de usuarios conectarse alrededor del mundo. Esta plataforma rompe las barreras culturales y de comunicación que anteriormente existían a falta de internet. En este sentido se pretende analizar a YouTube desde una perspectiva administrativa enfocada en el área de mercadeo.