Major histocompatibility complex class I-restricted T cells are required for all but the end stages of diabetes development in nonobese diabetic mice and use a prevalent T cell receptor α chain gene rearrangement

Nonobese diabetic (NOD) mice develop insulin-dependent diabetes mellitus due to autoimmune T lymphocyte-mediated destruction of pancreatic β cells. Although both major histocompatibility complex class I-restricted CD8+ and class II-restricted CD4+ T cell subsets are required, the specific role each subset plays in the pathogenic process is still unclear. Here we show that class I-dependent T cells are required for all but the terminal stages of autoimmune diabetes development. To characterize the diabetogenic CD8+ T cells responsible, we isolated and propagated in vitro CD8+ T cells from the earliest insulitic lesions of NOD mice. They were cytotoxic to NOD islet cells, restricted to H-2Kd, and showed a diverse T cell receptor β chain repertoire. In contrast, their α chain repertoire was more restricted, with a recurrent amino acid sequence motif in the complementarity-determining region 3 loop and a prevalence of Vα17 family members frequently joined to the Jα42 gene segment. These results suggest that a number of the CD8+ T cells participating in the initial phase of autoimmune β cell destruction recognize a common structural component of Kd/peptide complexes on pancreatic β cells, possibly a single peptide.

In vitro reconstitution of the photosystem I light-harvesting complex LHCI-730: Heterodimerization is required for antenna pigment organization

Here we describe the in vitro reconstitution of photosystem I light-harvesting complexes with pigments and proteins (Lhca1 and Lhca4) obtained by overexpression of tomato Lhca genes in Escherichia coli. Using Lhca1 and Lhca4 individually for reconstitution results in monomeric pigment-proteins, whereas a combination thereof yields a dimeric complex. Interactions of the apoproteins is highly specific, as reconstitution of either of the two constituent proteins in combination with a light-harvesting protein of photosystem II does not result in dimerization. The reconstituted Lhca1/4, but not complexes obtained with either Lhca1 or Lhca4 alone, closely resembles the native LHCI-730 dimer from tomato leaves with regard to spectroscopic properties, pigment composition, and stoichiometry. Monomeric complexes of Lhca1 or Lhca4 possess lower pigment/protein ratios, indicating that interactions of the two subunits not only facilitates pigment reorganization but also recruitment of additional pigments. In addition to higher averages of chlorophyll a/b ratios in monomeric complexes than in LHCI-730, comparative fluorescence and CD spectra demonstrate that heterodimerization involves preferential ligation of more chlorophyll b.

Earth science field trip, guide leaflet : Metropolis area : Massac, Pope and Johnson Counties : Paducah, Brownfield, Vienna and La Center Quadrangles.

"April 11, 1959."

Lezioni sopra i doveri e le qualita di un medico /

Mode of access: Internet.

I papi e le università /

"Ragionamento letto nella romana Accademia degli Arcadi il 16 giugno 1865."

Lezioni sopra i doveri e le qualità di un medico /

Mode of access: Internet.

Drei Denkschriften über die orientalische Frage von Papst Leo X, König Franz I von Frankreich und Kaiser Maximilian I aus dem Jahre, 1517;

Mode of access: Internet.

The poetical works of Alexander Pope

Contiene : Vol. I.

Pope Clement IX

Gian Lorenzo Bernini; 3 ft. 1 63/64 in. x 2 ft. 8 33/64 in. x 1 ft. 1/64 in. (with base); bronze

The monks of the West from St. Benedict to St. Bernard.

Original French edition published Paris, 1860-77, v. 5-6 being edited by Aure lien de Courson.

Ecclesiastical memorials, relating chiefly to religion, and the reformation of it, and the emergencies of the Church of England, under King Henry VIII. King Edward VI. and Queen Mary I. with large appendixes, containing original papers, records, &c. /

Binder's title: Strype's works, VIII-XIII.

The British satirist. Comprising the best satires of the most celebrated poets, from Pope to Byron. Accompanied by original critical notices of the authors.

Added t.-p., engraved, with vignette.

Discorso recitato nella basilica di S. Pietro in Vaticano nel dì I. agosto dell'anno 1815 /

Mode of access: Internet.

The angling excursions of Gregory Greendrake in Ireland.

Gregory Greendrake is a pseudonym attributed to J. Coad or Henry Brereton Cody.