Genetic structuring of Eunicella singularis populations along a bathymetric gradient

Phenotypic plasticity refers to the ability of an organism to express different morphologies depending on the abiotic and biotic environment. Depth integrating many variables (e.g. temperature, light and hydrodynamics), may affect population structure and dynamics of the populations, as well as connectivity patterns and genetic diversity. Eunicella singularis is a Mediterranean arborescent gorgonian who plays an important rule as engineer species providing biomass and complexity to coralligenous habitats. It has a wide bathymetric distribution ranging from shallow rocky bottoms to deep sublittoral reefs. The species shows two depth-related morphotypes which taxonomic status is not yet clarified. The aim of the study is to analyses genetic variability and/or structuring along a vertical gradient to test the presence of the two morphotypes. Furthermore, a preliminary analyses of the phylogenetic relationship among species of the genus Eunicella has been done. Six populations of Eunicella singularis were sampled from 10 to 60 m depth in Cap de Creus and individuals belonging to Eunicella cavolinii, E. verrucosa, E. racemosa and E. stricta aphyta were collected. The genetic analyses were carried out using five microsatellite loci and ITS-1 sequence polymorphism. The results showed a reduction of genetic variability along the vertical gradient. A threshold in connectivity was observed across 30 - 40 m depth, confirming the presence of two different Eunicella singularis morphotypes. The two morphological forms could be due to phenotypic plasticity, which allowed populations to suit different environmental conditions, or to a break in gene flow that determined the isolation of the two populations and an accumulation of genetic differences. The molecular markers used were not able to clarify the phylogenetic relationship among Eunicella species and the systematic position of the two morphotypes, conversely they risen the question on the existence of single species of Mediterranean Eunicella.

Combined moxidectin and environmental therapy do not eliminate Chorioptes bovis infestation in heavily feathered horses

Chorioptes bovis infestation is a common cause of pastern dermatitis in the horse, with a predilection in draft horses and other horses with thick hair 'feathers' on the distal limbs. The treatment of this superficial mite is challenging; treatment failure and relapse are common. Furthermore, C. bovis infestation may affect the progression of chronic pastern dermatitis (also known as chronic proliferative pastern dermatitis, chronic progressive lymphoedema and dermatitis verrucosa) in draft horses, manifesting with oedema, lichenification and excessive skin folds that can progress to verruciform lesions. An effective cure for C. bovis infestation would therefore be of great clinical value. In a prospective, double-blind, placebo-controlled study, the efficacy of oral moxidectin (0.4 mg/kg body weight) given twice with a 3 week interval in combination with environmental treatment with 4-chloro-3-methylphenol and propoxur was tested in 19 heavily feathered horses with clinical pastern dermatitis and C. bovis infestation. Follow-up examinations over a period of 180 days revealed significantly more skin crusting in the placebo group than in the treatment group. However, no other differences in clinical signs or the numbers of mites detected were found between the two groups. The results of this study suggest that moxidectin in combination with environmental insecticide treatment as used in this study is ineffective in the treatment of C. bovis in feathered horses.

Dinoflagellate and other palynomorph abundance in sediments from ODP Leg 189 sites

At Ocean Drilling Program (ODP) Leg 189 Sites 1170-1172, the climatologically critical Eocene-Oligocene (E-O) transition is barren of any calcareous microfossils but contains rich marine organic walled dinoflagellate cyst (dinocyst) and diatom assemblages, suitable for detailed biostratigraphic and paleoenvironmental analysis. The resulting first-ever integrated dinocyst/diatom magnetostratigraphy allows confident correlation of the E-O interval between all Leg 189 sites, including Site 1168. Our correlations indicate that the (deep) opening of the Tasmanian Gateway occurred quasi-synchronously throughout the Tasmanian region, starting at ~35.5 Ma. At Sites 1170-1172, quantitatively, three distinct dinocyst assemblages may be distinguished that reflect the relatively rapid and pronounced stepwise environmental changes associated with the E-O transition in the Tasmanian region, from a pro-deltaic setting to a deep marine pelagic setting. Moreover, synchronous with the deepening of the gateway, at the southern and eastern Sites 1170-1172, typical endemic Antarctic assemblages were replaced by more cosmopolitan dinocyst communities. In marked contrast, at Site 1168 off western Tasmania, endemic Antarctic taxa are virtually absent during the E-O transition. At Sites 1170-1172, the endemic Antarctic dinocyst assemblage (Transantarctic Flora) drastically changes into a more cosmopolitan assemblage at ~35.5 Ma, with a more offshore character, reflecting the arrival of different oceanographic and environmental conditions associated with the deepening of the Tasmanian Gateway. In turn, this assemblage grades at ~34 Ma into one more typical for even more offshore and/or upwelling conditions at Site 1172. In slightly younger deposits at all sites, organic microfossils are virtually absent, reflecting winnowing and oxidation, indicative of a next step of oceanographic development. This phase may be dated as close to the Oceanic Anoxic (Oi)-1 18O (Antarctic glaciation) event (~33.3 Ma). In a single productive sample from the earliest Oligocene the northern Site 1172, a relatively warm-water cosmopolitan assemblage has been recovered. This aspect contrasts findings from coeval deposits from the Ross Sea, where endemic Antarctic species remain dominant. Somewhere between the paleogeographic positions of Site 1172 and the Ross Sea, a strong differentiation of surface waters occurred in the earliest Oligocene, possibly reflecting the onset of the Antarctic Circumpolar Current.

Dinoflagellate cysts abundance and events of ODP Hole 189-1168A sediments

Palynomorphs were studied in samples from Ocean Drilling Program (ODP) Leg 189, Hole 1168A (slope of the western margin of Tasmania; 2463 m water depth). Besides organic-walled dinoflagellate cysts (dinocysts), broad categories of other palynomorphs were quantified in terms of relative abundance. In this contribution, we provide an overview of the early late Eocene-Quaternary dinocyst distribution and illustrate main trends in palynomorph distribution. Dinocyst species throughout Hole 1168A are largely cosmopolitan with important contributions of typical low-latitude taxa and virtual absence of endemic Antarctic taxa. Dinocyst stratigraphic distribution broadly matches that known from the Northern Hemisphere and equatorial regions, although significant differences are noted. Selected potentially biochronostratigraphically useful events are summarized. The distribution of dinocysts in the middle-upper Miocene interval is rather patchy, probably due to prolonged exposure to oxygen. An important general aspect in the dinocyst assemblages is the near absence of Antarctic endemic species and the apparent influence of relatively warm waters throughout the succession at Site 1168. General palynomorph distribution indicates continued deepening from an initial shallow, even restricted, marine setting from late Eocene-Quaternary times. A curious massive influx of small skolochorate acritarchs is recorded throughout the late early-early middle Miocene; the significance of this signal is not yet understood. A general long-term oligotrophic nature of the surface waters influencing Site 1168 is suggested from the low abundance of (proto) peridinioid, presumably heterotrophic, species. The overall dinocyst distribution pattern corresponds to the long-term existence of a Leeuwin-like current influencing the region, including Site 1168, confirming results of earlier studies on other microfossil groups. The occasional influence of colder surface water conditions is, however, also apparent, notably during the late Pliocene-Quaternary, indicating the potential of high-resolution dinocyst analysis for future paleoceanographic studies.

Benthos fauna in the North Sea from 2 surveys in 1986 and 1987

During two surveys in the North Sea, in summer 1986 and in winter 1987, larger epibenthos was collected with a 2 m beam trawl. The distributions of the species were checked for average linkage by means of the JACCARD-index cluster analysis. In summer two main clusters can be recognized. These are situated to the north and to the south of the Dogger Bank. In winter two main clusters may be recognized as well, but these clusters divide the North Sea into a western and an eastern part. We conclude, that these differences of epibenthos characteristics are correlated with seasonal changes in water body distributions.

Ascidiofauna in the Southern Ocean

Three Spanish Antarctic research cruises (Ant-8611, Bentart-94 and Bentart-95) were carried out in the South Shetland Archipelago (Antarctic Peninsula) and Scotia Arc (South Orkney, South Sandwich and South Georgia archipelagos) on the continental shelf and upper slope (10-600 m depth). They have contributed to our knowledge about ascidian distribution and the zoogeographical relationships with the neighbouring areas and the other Subantarctic islands. The distribution of ascidian species suggests that the Scotia Arc is divided into two sectors, the South Orkney Archipelago, related to the Antarctic Province, and the South Georgia Archipelago (probably including the South Sandwich Archipelago), which is intermediate between the Antarctic Province and the Magellan region.

Late Cretaceous dinoflagellate cysts of ODP Hole 120-748C

At Ocean Drilling Program Hole 748C in the Southern Indian Ocean, a total of 171 Late Cretaceous dinoflagellate taxa were encountered in 38 productive samples from Cores 120-748C-27R through 120-748C-62R (407-740 mbsf). Four provisional dinoflagellate assemblage zones and five subzones were recognized based on the character of the dinoflagellate flora and the first/last occurrences of some key species. Isabelidinium korojonense and Nelsoniella aceras occur in Zone A together with Oligosphaeridium pulcherrimum and Trithyrodinium suspect urn. Zone B was delineated by the total range of Odontochitina cribropoda. Zone C was separated from Zone B by the presence of Satyrodinium haumuriense, and Zone D is dominated by new taxa. The dinocyst assemblages bear a strong affinity to Australian assemblages. Paleoenvironmental interpretations based mainly on dinocysts suggest that during the ?Santonian-Campanian to the Maestrichtian this portion of the Kerguelen Plateau was a shallow submerged plateau, similar to nearshore to offshore to upper slope environments with water depths of tens to hundreds of meters, but isolated from the major continents of the Southern Hemisphere. Starting perhaps in the late Cenomanian (Mohr and Gee, 1992, doi:10.2973/odp.proc.sr.120.196.1992), the Late Cretaceous transgression over the plateau reached its maximum during the late Campanian. The plateau may have been exposed above sea level and subjected to weathering during the latest Maestrichtian. The studied dinocyst assemblages characterized by species of Amphidiadema, Nelsoniella, Satyrodinium, and Xenikoon together with abundant Chatangiella (the large-size species) and Isabelidinium suggest that a South Indian Province (tentatively named the Helby suite) may have existed during the Campanian-Maestrichtian in comparison with the other four provinces of Lentin and Williams. One new genus, three new species, and two new subspecies of dinocysts are described.

Microfossils detected in a block of ancient dense clay coated with a ferromanganese crust, Clarion-Clipperton Province, East Equatorial Pacific

The area in study is characterized by a regional stratigraphic hiatus from Early Miocene to Quaternary. Deposits from Late Eocene to Early Miocene occur on the bottom surface or under a thin sedimentary cover. Ferromanganese nodules, mostly of Oligocene age, formed on surface layers of Tertiary or Quaternary sediments. A detailed micropaleontological study of a block of dense ancient clay coated with a ferromanganese crust was carried out. Composition of found radiolarian and diatomaceous complexes proved that the crust formed in Quaternary on an eroded surface of Late Oligocene clay. In Quaternary Neogene sediments were eroded and washed away by bottom currents. It is likely that the erosion began 0.9-0.7 Ma at the beginning of the "Glacial Pleistocene". The erosion could be initiated by loosening and resuspension of surface sediments resulting from seismic activity generated by strong earthquakes in the Central America subduction zone. The same vibration maintained residual nodules at the seafloor surface. Thus, for the area in study a common reason and a common Quaternary interval for formation of the following features is supposed: a regional stratigraphic hiatus, formation of residual nodule fields, and position of ancient nodules on the surface of Quaternary sediments.

Dinoflagellate abundance and events of ODP Site 189-1172 sediments

Palynomorphs were studied in samples from Ocean Drilling Program (ODP) Leg 189, Holes 1172A and 1172D (East Tasman Plateau; 2620 m water depth). Besides organic walled dinoflagellate cysts (dinocysts), broad categories of other palynomorphs were quantified in terms of relative abundance. In this contribution, we provide an overview of the dinocyst distribution from the Maastrichtian to lowermost Oligocene and Quaternary intervals and illustrate main trends in palynomorph distribution. The uppermost Cretaceous-lowermost Oligocene succession of Site 1172 has a confident biomagnetostratigraphy, enabling us to tie early Paleogene Southern Hemisphere dinocyst events to the geomagnetic polarity timescale for the first time. Dinocyst species from the Maastrichtian to earliest Oligocene at Site 1172 are largely endemic ("Transantarctic Flora") or bipolar; cosmopolitan taxa are present in the background as well. The Maastrichtian-early late Eocene dinocyst assemblages are indicative of shallow-marine to restricted marine, pro-deltaic conditions, closely tied to a massive siliciclastic sequence. By middle late Eocene times (~35.5 Ma), the siliciclastic sequence gave way to a thin glauconitic unit, considered to reflect the deepening of the Tasmanian Gateway. This transition coincides with the most prominent change in dinocyst associations of the Paleogene. The turnover is inferred to reflect a change from marginal marine to more offshore conditions, with increased winnowing and oxidation. Overlying pelagic carbonate ooze of middle early Oligocene and younger age is virtually barren of organic microfossils, although Quaternary assemblages have been recovered. This aspect is taken to reflect average low sedimentation rates and well-oxygenated water masses during most of the Oligocene and Neogene. The few palynologically productive samples from the Oligocene-Quaternary interval have a stronger cosmopolitan to subtropical signature, with warm-water species being common to abundant.

Calcium isotope fractionation in cultured, modern and fossil scleractinian coral skeleton

The present study investigates the influence of environmental (temperature, salinity) and biological (growth rate, inter-generic variations) parameters on calcium isotope fractionation (d44/40Ca) in scleractinian coral skeleton to better constrain this record. Previous studies focused on the d44/40Ca record in different marine organisms to reconstruct seawater composition or temperature, but only few studies investigated corals. This study presents measurements performed on modern corals from natural environments (from the Maldives for modern and from Tahiti for fossil corals) as well as from laboratory cultures (Centre Scientifique de Monaco). Measurements on Porites sp., Acropora sp., Montipora verrucosa and Stylophora pistillata allow constraining inter-generic variability. Our results show that the fractionation of d44/40Ca ranges from 0.6 to 0.1 per mil, independent of the genus or the environmental conditions. No significant relationship between the rate of calcification and d44/40Ca was found. The weak temperature dependence reported in earlier studies is most probably not the only parameter that is responsible for the fractionation. Indeed, sub-seasonal temperature variations reconstructed by d18O and Sr/Ca ratio using a multi-proxy approach, are not mirrored in the coral's d44/40Ca variations. The intergeneric variability and intrageneric variability among the studied samples are weak except for S. pistillata, which shows calcium isotopic values increasing with salinity. The variability between samples cultured at a salinity of 40 is higher than those cultured at a salinity of 36 for this species. The present study reveals a strong biological control of the skeletal calcium isotope composition by the polyp and a weak influence of environmental factors, specifically temperature and salinity (except for S. pistillata). Vital effects have to be investigated in situ to better constrain their influence on the calcium isotopic signal. If vital effects could be extracted from the isotopic signal, the calcium isotopic composition of coral skeletons could provide reliable information on the calcium composition and budget in ocean.

Soft-bottom macrobenthic faunal associations in the southern Chilean glacial fjord complex

Macrobenthic associations were investigated at 29 sampling stations with a semi-quantitative Agassiz trawl, ranging from the South Patagonian Icefield to the Straits of Magellan in the South Chilean fjord system. A total of 1,895 individuals belonging to 131 species were collected. 19 species belong to colonial organisms, mainly Bryozoa (17 species) and Octocorallia (2 species). The phylum Echinodermata was the most diverse in species number (47 species), with asteroids (25 species) and ophiuroids (13 species) being the best represented within this taxon. Polychaeta was the second dominant group in terms of species richness (46 species). Multidimensional scaling ordination (MDS) separated two station groups, one related to fjords and channels off the South Patagonian Icefield and the second one to stations surrounding the Straits of Magellan. 45 species account for 90% of the dissimilarity between these two groups. These differences can mainly be explained by the influence of local environmental conditions determined by processes closely related to the pres- ence/absence of glaciers. Abiotic parameters such as water depth, type of sediment and chemical features of the superficial sediment were not correlated with the numbers of individuals caught by the Agassiz trawl in each group of sampling stations.

Bolboforma abundance in ODP Hole 162-982A and DSDP Hole 12-116 sediments

Although they are fossils of uncertain origin, bolboforms are the best calcareous microfossil group for Neogene biostratigraphy in the North Atlantic. Fifty-two Bolboforma species were observed at the Hatton-Rockall Basin in Ocean Drilling Program Holes 982A (26 samples) and 982B (301 samples) and in Deep Sea Drilling Project Hole 116 (71 samples). The sequence investigated spans the interval from lower Miocene to upper Pliocene. Fourteen zones/subzones were identified and correlated with the calcareous nannoplankton zones, the planktonic foraminifer biostratigraphy, and the time (Ma). The last occurrence of the genus Bolboforma can be dated to 2.84 Ma. Different Bolboforma specimens of middle Miocene age, observed in upper Miocene and upper middle Miocene sediments at Site 982, document redeposition of sediment from the Rockall Bank into the Hatton-Rockall Basin during the latest middle Miocene and late Miocene.