Medium density particleboard reinforced with bamboo laminas

The objective of this work was to evaluate the effect of the addition of bamboo laminas of the species Dendrocalamus giganteus to three-layer medium density particleboard (MDP). These laminas were glued onto both the top and the bottom of each panel. With the manufactured panels laminated with bamboo, mechanical tests based on the Brazilian Standard ABNT NBR 14810 were carried out to determine the modulus of rupture (MOR) in static bending and the tensile strength parallel-to-surface. These mechanical tests were realized in particleboards of eucalyptus and in reinforced particleboard, both produced in the laboratory. The modulus of rupture and tensile strength parallel-to-surface of the laminated MDP had values close to those that have been reported. The reinforcements increased the values of these studied properties. Nevertheless, this fact indicated the possibility to produce a stronger MDP using bamboo lamina as surface layers. These results show the possibility of using coatedbamboo MDP for utilization in large spans, for example, in flooring for mezzanines with finish on both sides, and for robust furniture as bookshelves, beds, tables, etc.

SOCIAL MONOGAMY AND BIPARENTAL CARE OF THE NEOTROPICAL SOUTHERN BAMBOO RAT (KANNABATEOMYS AMBLYONYX)

We analyzed social patterns indicative of the mating system and parental care in a population of the southern bamboo rat (Kannabateomys amblyonyx). This arboreal rodent feeds exclusively on bamboo stems and leaves. We conducted fieldwork from August 2003 to October 2004 in southern Brazil (30 degrees 20`-30 degrees 27`S, 50 degrees 50`-51 degrees 05`W), in patches of introduced Chinese bamboo (Bambusa tuldoides). We captured 18 individuals, 7 of which were adults that received radiotransmitters and were followed from 1 to 12 months. Another 5 animals (adults or subadults) received colored collars. We observed paternal care, delayed juvenile dispersal, and reduced degree of sexual dimorphism, all of which are traits typical of social monogamy. Mated males showed a direct parental behavioral repertoire similar to that of females (with the obvious exception of nursing), including grooming, huddling, and food provisioning. Potential monogamy in this species seems to be a flexible strategy linked to low density of bamboo patches. Females were confined to widely spaced., small home ranges, decreasing the possibility of male defense of and access to > 1 female. The arboreal habits of the species possibly increase the risk of inexperienced young falling from trees or else being depreciated when moving exposed through branches. This risk is probably reduced by the extensive biparental care observed, including the providing of low-energy plant food to young in the nest.

Characterization and properties of blended cement matrices containing activated bamboo leaf wastes

The worldwide production of bamboo generates large volumes of leaf wastes, which are deposited in landfills or burned in an uncontrolled manner, with negative effects in the environment. The ash obtained by calcining of the bamboo leaf waste, shows good qualities as supplementary cementing material for the production of blended cements. The current paper shows a detailed scientific study of a Brazilian bamboo leaf ash (BLA) calcined at 600 degrees C in small scale condition, by using different techniques (XRF, XRD, SEM/EDX, FT-IR, TG/DTG) and technical study in order. to analyse the behaviour of this ash in blended cements elaborated with 10% and 20% by mass of BLA. The results stated that this ash shows a very high pozzolanic activity, with a reaction rate constant K of the order of 10(-1)/h and type I CSH gel was the main hydrated phase obtained from pozzolanic reaction. The BLA blended cements (10% and 20%) complied with the physical and mechanical requirements of the existing European standards. (c) 2012 Elsevier Ltd. All rights reserved.

Structure–property relationships of elementary bamboo fibers

Natural fibers are promising alternatives to synthetic fibers because of their sustainability, low environmental footprint and specific properties desirable for a wide range of technical engineering applications. The industrial implementation of fine grade natural bamboo fibers, including technical (100–200 microns) and elementary fibers (<30 microns) has been of increasing interest in recent times because these fibers offer a unique set of properties including high tensile strength, antibacterial and UV absorption. However to date, very little scientific effort has been devoted to fully understand the inter-correlation between their mechanical, physico-chemical, microstructural and morphological properties. In this paper, we report for the first time the structure–property relationship of elementary bamboo fibers. The impact of the inner microstructural organization of fibers (including the micro-fibrils angle) and physico-chemical factors such as the cellulose content and crystallinity index, on the tensile performance of these fibers is discussed in detail. This work also provides an insight into the application of bamboo fibers as natural and low-cost sorbent material for the removal of Cu2+ metal ions from model industrial wastewater. The metal ion adsorption properties of the fibers are correlated to surface energy analysis obtained from inverse gas chromatography.

Expression and distribution of cell-surface proteoglycans in the normal Lewis rat molar periodontium

Cell-surface proteoglycans participate in several biological functions such as cell cell and cell-matrix interactions, cell adhesion, the binding to various growth factors as co-receptors and repair. To understand better the expression and distribution of cell-surface proteoglycans in the periodontal tissues, an immunohistochemical evaluation of the normal Lewis rat molar periodontium using panels of antibodies for syndecan-1, -2, -4, glypican and betaglycan was carried out. Our results demonstrated the expression and distribution of all proteoglycans in the suprabasal gingival epithelium, soft and hard connective tissues. Both cellular and matrix localization was evident within the various periodontal compartments. The presence of these cell-surface proteoglycans indicates the potential for roles in the process of tissue homeostasis, repair or regeneration in periodontium of which each function requires further study.

Micro-scale anaerobic digestion of point source components of organic fraction of municipal solid waste

The fermentation characteristics of six specific types of the organic fraction of municipal solid waste (OFMSW) were examined, with an emphasis on properties that are needed when designing plug-flow type anaerobic bioreactors. More specifically, the decomposition patterns of a vegetable (cabbage), fruits (banana and citrus peels), fresh leaf litter of bamboo and teak leaves, and paper (newsprint) waste streams as feedstocks were studied. Individual OFMSW components were placed into nylon mesh bags and subjected to various fermentation periods (solids retention time, SRT) within the inlet of a functioning plug-flow biogas fermentor. These were removed at periodic intervals, and their composition was analyzed to monitor decomposition rates and changes in chemical composition. Components like cabbage waste, banana peels, and orange peels fermented rapidly both in a plug-flow biogas reactor (PFBR) as well as under a biological methane potential (BMP) assay, while other OFMSW components (leaf litter from bamboo and teak leaves and newsprint) fermented slowly with poor process stability and moderate biodegradation. For fruit and vegetable wastes (FVW), a rapid and efficient removal of pectins is the main cause of rapid disintegration of these feedstocks, which left behind very little compost forming residues (2–5%). Teak and bamboo leaves and newsprint decomposed only to 25–50% in 30 d. These results confirm the potential for volatile fatty acids accumulation in a PFBR’s inlet and suggest a modification of the inlet zone or operation of a PFBR with the above feedstocks.

The influence of buoyancy on the oscillatory thermocapillary convection with small bond number

This work is an experimental study of unidirectional bamboo-epoxy laminates of varying laminae number, in which tensile, compressive, flexural and interlaminar shear properties are evaluated. Further, the disposition of bamboo fibre, the parenchymatous tissue, and the resin matrix under different loading conditions are examined. Our results show that the specific strength and specific modulus of bamboo-epoxy laminates are adequate, the former being 3 to 4 times that of mild steel. Its mechanical properties are generally comparable to those of ordinary glass-fibre composites. The fracture behaviour of bamboo-epoxy under different loading conditions were observed using both acoustic emission techniques and scanning electron microscopy. The fracture mode varied with load, the fracture mechanism being similar to glass and carbon reinforced composites. Microstructural analyses revealed that natural bamboo is eligibly a fibre composite in itself; its inclusion in a plastic matrix will help solve the problems of cracking due to desiccation and bioerosion caused by insect pests. Furthermore, the thickness and shape of the composite can be tailored during fabrication to meet specific requirements, thereby enabling a wide spectrum of applications.

A scientific forum on the Gulf of Mexico: The Islands in the Stream Concept. Proceedings of the Forum: 23 January 2008, Keating Education Center, Mote Marine Laboratory, Sarasota, Florida

The Scientific Forum on the Gulf of Mexico: The Islands in the Stream Concept took place in January 2008 in Sarasota, Florida. The purpose of the meeting was to bring together scientists and managers from around the Gulf of Mexico to discuss a range of topics on our knowledge of the Gulf of Mexico, from its geology to larger-scale connectivity to the Caribbean region, and their applications to the concept of a more integrated approach to area-based management. The forum included six panels of invited experts who spoke on the oceanographic and biological features in the Gulf of Mexico, including connections with Mexico and the Mesoamerican barrier reef system, and the legal and regulatory structure currently in place. The charge to the group was to share information, identify gaps in our knowledge, identify additional potential areas for protection, and discuss available science about connectivity and the potential value of establishing a marine protected area network in the Gulf of Mexico. (PDF has 108 pages.)

Prioritization of candidate cancer genes-an aid to oncogenomic studies

The development of techniques for oncogenomic analyses such as array comparative genomic hybridization, messenger RNA expression arrays and mutational screens have come to the fore in modern cancer research. Studies utilizing these techniques are able to highlight panels of genes that are altered in cancer. However, these candidate cancer genes must then be scrutinized to reveal whether they contribute to oncogenesis or are coincidental and non-causative. We present a computational method for the prioritization of candidate (i) proto-oncogenes and (ii) tumour suppressor genes from oncogenomic experiments. We constructed computational classifiers using different combinations of sequence and functional data including sequence conservation, protein domains and interactions, and regulatory data. We found that these classifiers are able to distinguish between known cancer genes and other human genes. Furthermore, the classifiers also discriminate candidate cancer genes from a recent mutational screen from other human genes. We provide a web-based facility through which cancer biologists may access our results and we propose computational cancer gene classification as a useful method of prioritizing candidate cancer genes identified in oncogenomic studies.

Ecological factors related to the distribution of Bankia gouldi Bartsch in Chesapeake Bay

Organisms were collected on test panels, six inch lengths of dressed two by four inch pine, suspended in the water in a vertical position as described by Turner (1947). The panels were usually located at some convenient structure such as a dock-piling or sea-wall. Except where otherwise indicated by the data, the samples were collected from each station once a month between May 1950 and May 1953. During the three year period, seven hundred and nineteen panels were submerged in Chesapeake Bay. Approximately 14,000 organisms were encountered on these panels of which 20% or approximately 3,000 organisms could be identified from the dried pallets. Preliminary notes on the extent of fouling were made in the field after which the samples were removed to the laboratory for further study.

Quality assessment of rotating and solar tunnel dried marine fish product

Studies were conducted to evaluate the quality aspects of marine dried fish i.e. silver jew fish (Johnius argentatus), Bombay duck (Harpodon nehereus) and ribbon fish (Trichiums haumela) products produced in rotating and solar tunnel dryers. On the basis of organoleptic characteristics such as colour, odour, texture, broken pieces, insect infestation and overall quality, four member panels of experts evaluated the quality of the dried products obtained from both rotating and solar tunnel dryers and all the products were found in acceptable quality. Reconstitutions properties of samples were in the range of 51.05 to 98.75% for the dried fish produced in rotating dryer, while 24.64 to 76.76% for dried fish produced in solar tunnel dryer. The highest reconstitution rate was found in dried silver jew fish and lowest in ribbon fish produced in rotating dryer. On the other hand, the highest reconstitution was observed in dried silver jew fish and lowest in dried Bombay duck produced in solar tunnel dryer. Proximate composition such as moisture, crude protein, lipid and ash content of the dried fish muscles produced in rotating dryer ranged from 16.36% to 19.1%, 62.35% to 67.37%, 6.37% to 10.75% and 7.00% to 8.05%, respectively and in solar tunnel dried fish products, they were in the range of 14.05% to 19.71%,57.64% to 69. 21%,6.92% to 15.40%and 7.69% to 8.80 %, respectively. The TVBN values of dried fish products obtained from rotating dryer were in the range of 15.02 to 19.05 mg/100g, while in solar tunnel dried fish products, the values were in the range of 15.46 to 19.21 mg/100g. The results of the studies indicated that dried fish produced from both rotating and solar tunnel drier were acceptable quality in terms of organoleptic and food quality aspects.

Effects of black Bengal goat manure on growth and production of Genetically Improved Farmed Tilapia (GIFT) starin [sic], Oreochromis niloticus

In a goat-tilapia integrated farming system, the effect of Black Bengal goat manure on the growth and production of Oreochromis niloticus was studied at the Freshwater Substation, Shantahar, Bogra for 4.5 months. The stocking density used in three treatments were, 200 goats and 15,000 GIFT strain/ha (T1); 300 goats and 15,000 GIFT strain/ha (T2); and only 15,000 GIFT strain/ha (T3). The initial individual total length and weight of stocked tilapia were 7.6 cm and 11.34 g, respectively. Twelve ponds each having an area of 40 square meters were used for this trail [sic]. On one side of each pond goat shed was constructed and the space allocated for each goat was 0.75 m x 1.5 m. Newly weaned black Bengal goats of average weight 8.45 kg was used in the trial. Every morning the goats manure was swiped out in ponds through the fixed opening of bamboo made floor of goat shed. Water depth of the ponds was maintained at 0.75 m. The highest fish production was in treatment 1 (1,750 kg/ha) followed by treatment 2 (1,455 kg/ha) and treatment 3 (621 kg/ha). The difference in individual goat weight was not significant (P>0.05) among treatments 1 and 2.

Assessment of the immunomodulatory effects of raw/farm milk and probiotic bacteria

The overall aims of this study were to investigate the differences between raw/farm milk and pasteurised milk with respect to potential immune modifying effects following consumption and investigate the bacterial composition of raw milk compared to pasteurised milk. Furthermore, in this thesis, panels of potential probiotic bacteria from the Bifidobacterium and Lactobacillus genera were investigated. The overall bacterial composition of raw milk was compared with pasteurised milk using samples obtained from commercial milk producers around Ireland using next generation sequencing technology (454 pyrosequencing). Here the presence of previously unrecognised and diverse bacterial populations in unpasteurised cow’s milk was identified. Futhermore the bacterial content of pasteurised milk was found to be more diverse than previously thought. The global response of the adenocarcinoma cell line HT-29 to raw milk and pasteurised milk exposures were also characterised using whole genome microarray technology. Over one thousand differentially expressed genes were identified which were found to be involved in a plethora of cellular functions. Interestingly a reduction in immune related activity (e.g. Major histocompatability complex class II signalling and T and B cell proliferation) was identified in cells exposed to pasteurised milk compared with raw milk exposures. Further studies comparing human cell response to raw versus pasteurised milk was performed using peripheral blood mononuclear cells (PBMC) from healthy donors. A reduction in CD14 was identified following raw milk exposures compared with pasteurised milk and the pattern of cytokine production may indicate that gram positive bacteria in the raw milk were contributing to the differences in the cellular response to raw versus pasteurised milk. Panels of potentially probiotic bacteria (comprising of lactobacilli and bifidobacteria) were further assessed for immunomodulatory capabilities using cell culture based models. Gene expression and cytokine production were used to evaluate stimulated and unstimulated (LPS) cellular responses as well as interaction mechanisms

Potent and broad neutralizing activity of a single chain antibody fragment against cell-free and cell-associated HIV-1.

Several human monoclonal antibodies (hmAbs) exhibit relatively potent and broad neutralizing activity against HIV-1, but there has not been much success in using them as potential therapeutics. We have previously hypothesized and demonstrated that small engineered antibodies can target highly conserved epitopes that are not accessible by full-size antibodies. However, their potency has not been comparatively evaluated with known HIV-1-neutralizing hmAbs against large panels of primary isolates. We report here the inhibitory activity of an engineered single chain antibody fragment (scFv), m9, against several panels of primary HIV-1 isolates from group M (clades A-G) using cell-free and cell-associated virus in cell line-based assays. M9 was much more potent than scFv 17b, and more potent than or comparable to the best-characterized broadly neutralizing hmAbs IgG(1) b12, 2G12, 2F5 and 4E10. It also inhibited cell-to-cell transmission of HIV-1 with higher potency than enfuvirtide (T-20, Fuzeon). M9 competed with a sulfated CCR5 N-terminal peptide for binding to gp120-CD4 complex, suggesting an overlapping epitope with the coreceptor binding site. M9 did not react with phosphatidylserine (PS) and cardiolipin (CL), nor did it react with a panel of autoantigens in an antinuclear autoantibody (ANA) assay. We further found that escape mutants resistant to m9 did not emerge in an immune selection assay. These results suggest that m9 is a novel anti-HIV-1 candidate with potential therapeutic or prophylactic properties, and its epitope is a new target for drug or vaccine development.

Comparative immunogenicity of HIV-1 clade C envelope proteins for prime/boost studies.

BACKGROUND: Previous clinical efficacy trials failed to support the continued development of recombinant gp120 (rgp120) as a candidate HIV vaccine. However, the recent RV144 HIV vaccine trial in Thailand showed that a prime/boost immunization strategy involving priming with canarypox vCP1521 followed by boosting with rgp120 could provide significant, although modest, protection from HIV infection. Based on these results, there is renewed interest in the development of rgp120 based antigens for follow up vaccine trials, where this immunization approach can be applied to other cohorts at high risk for HIV infection. Of particular interest are cohorts in Africa, India, and China that are infected with clade C viruses. METHODOLOGY/PRINCIPAL FINDINGS: A panel of 10 clade C rgp120 envelope proteins was expressed in 293 cells, purified by immunoaffinity chromatography, and used to immunize guinea pigs. The resulting sera were collected and analyzed in checkerboard experiments for rgp120 binding, V3 peptide binding, and CD4 blocking activity. Virus neutralization studies were carried out with two different assays and two different panels of clade C viruses. A high degree of cross reactivity against clade C and clade B viruses and viral proteins was observed. Most, but not all of the immunogens tested elicited antibodies that neutralized tier 1 clade B viruses, and some sera neutralized multiple clade C viruses. Immunization with rgp120 from the CN97001 strain of HIV appeared to elicit higher cross neutralizing antibody titers than the other antigens tested. CONCLUSIONS/SIGNIFICANCE: While all of the clade C antigens tested were immunogenic, some were more effective than others in eliciting virus neutralizing antibodies. Neutralization titers did not correlate with rgp120 binding, V3 peptide binding, or CD4 blocking activity. CN97001 rgp120 elicited the highest level of neutralizing antibodies, and should be considered for further HIV vaccine development studies.