Capillostrongyloides arapaimae sp. n. (Nematoda: Capillariidae), a new intestinal parasite of the arapaima Arapaima gigas from the Brazilian Amazon

A new nematode species, Capillostrongyloides arapaimae sp. n., is described from the intestine and pyloric caeca of the arapaima, Arapaima gigas (Schinz), from the Mexiana Island, Amazon river delta, Brazil. It is characterized mainly by the length of the spicule (779-1,800 µm), the large size of the body (males and gravid females 9.39-21.25 and 13.54-27.70 mm long, respectively) and by the markedly broad caudal lateral lobes in the male. It is the third species of genus Capillostrongyloides reported to parasitize Neotropical freshwater fishes.

Composição ictiofaunística e estratégias reprodutivas de quatro espécies de peixes nativos da Bacia Hidrográfica Piranhas - Assu, Rio Grande do Norte

This study focuses on the fish fauna composition and reproductive strategies of four native fish species in relation to environmental variables of the Piranhas-Assu hydrographic basin of the Caatinga biome, Rio Grande do Norte, Brazil. Fish and environmental data were collected monthly during the period September 2008 to February 2010, in the Marechal Dutra reservoir and River Acauã a tributary of Piranhas- Assu. The fish were measured, weighed and dissected, and the gonads were removed, weighed and examined macroscopically for identification and determination of gonad maturation stages. The results of this work generated seven articles. The first article is about the fish fauna composition of the Piranhas-Assu hydrographic basin, Rio Grande do Norte. The 602 fish samples captured were distributed in four orders (Characiformes, Perciformes, Siluriformes and Synbranchiformes), 11 families and 22 species, of which 17 are endemic to the Caatinga ecoregion. The order Characiformes was more representative followed by Perciformes, Siluriformes and Synbranchiformes. The second article is about the length-weight relationship and growth of seven native fish species Crenicichla menezesi, Cichlasoma orientale, Triportheus angulatus, Psectrogaster rhomboides, Pimelodella gracilis, Prochilodus brevis and Leporinus piau from a semiarid Brazilian reservoir. The third article is about the reproductive aspects of Crenicichla menezesi. Males were larger, heavier and with a slight predominance as compared to females. Four stages of gonadal development were characterized, being immature, maturing, mature and spent. The females reached sexual maturity earlier than males, with an average fecundity of 398 oocytes per batch. The spawning was partial with a long reproductive period. The fourth article deals with the reproductive strategy of Leporinus piau a neotropical freshwater fish in semi-arid region of Brazil. The population of L. piau (n = 211) showed a slight predominance of males (55%), with larger and heavier females. The males matured earlier than the females. This species presented total spawning, with an average fecundity of 55,000 mature oocytes. Rainfall and concentration of dissolved oxygen acted as influential factors during the spawning season. L. piau shows a seasonal reproductive strategy. The fifth article is related to the morphometric-meristic characteristics and reproductive aspects of freshwater sardine, Triportheus angulatus from River Acauã of the Caatinga biome. There was a predominance of larger females, reaching first maturation before males. There was total spawning during the rainy period of the region. The sixth article reports on the reproductive strategy of Psectrogaster rhomboides. The sex ratio was 1M: 1F, with negative allometric growth. Males reached sexual maturity earlier than females Females and males showed four stages of gonadal development and spawned during the rainy season. The fecundity was low and this species presented total spawning. The seventh article reports on the dynamics of territorial behavior of Crenicichla menezesi. Ten agonistic behaviors displayed by the males were observed: frontal and lateral threat, chasing, circular chasing, perpendicular, lateral and mouth attacks, escape, parallel positioning and stationary. The formation of four social groups was observed among males: without interaction; interaction with submission and escape; with frontal and lateral agonistic interactions. In social interactions between males and females, it was observed that the larger males interacted more among themselves and with the larger females. The large male established its territory and the two small males along with the small female were excluded from the other groups. These studies clarified the fish fauna composition and reproductive strategies of four native species Crenicichla menezesi, Leporinus piau, Triportheus angulatus and Psectrogaster rhomboides of the Piranhas- Assu hydrographic basin of the Caatinga biome, Rio Grande do Norte, Brazil.

Arraias em rios da região Sudeste do Brasil: locais de ocorrência e impactos sobre a população

INTRODUÇÃO: No presente trabalho objetivamos descrever o processo de colonização da Bacia do Alto Paraná, Sudeste do Brasil, por arraias, demonstrando sua atual situação e provável tendência, os impactos gerados e discutindo algumas ações de manejo e medidas mitigadoras. MÉTODOS: Foram realizadas entrevistas com ribeirinhos e profissionais de saúde para o levantamento de informações sobre a ocorrência de arraias e acidentes associados a estes animais, além de coletas e observações subaquáticas de potamotrigonídeos, entre 2004 e 2009, em localidades situadas nos Estados de São Paulo, Paraná e Mato Grosso do Sul, no Sudeste, Sul e parte do Centro-Oeste do Brasil. RESULTADOS: Três espécies de arraias foram identificadas na área de estudo, demonstrando utilizar os caminhos abertos pela Hidrovia Tietê-Paraná para se dispersarem. Dezesseis vítimas de acidentes envolvendo esses animais foram encontradas, notadamente banhistas e pescadores, chamando a atenção pelo fato dos casos não serem notificados e apresentarem elevada morbidade, com marcante incapacidade temporária para o trabalho. CONCLUSÕES: Este é o primeiro relato de invasão biológica envolvendo espécies de elasmobrânquios conhecido na literatura e, pelas arraias estarem colonizando áreas densamente povoadas e ampliando sua área de distribuição a cada ano, é de se esperar que sua interação negativa com humanos se intensifique, provocando alterações importantes no perfil epidemiológico dos acidentes por animais peçonhentos ocorridos no sudeste brasileiro.

A standard protocol for obtaining fish chromosomes under post-mortem conditions

In order to improve cytogenetical analyses on fish, especially focusing on delicate and rare species, we have adopted a new in vitro methodology using dead animals. The results obtained from 24 neotropical freshwater and marine fish species demonstrate that chromosomes can be obtained under post-mortem conditions. Significantly, the samples analyzed provided reliable cytogenetical data in nearly all cases. Other advantages of this new methodology are also discussed. (c) 2006 Elsevier Ltd. All rights reserved.

Chromosome evolution in fish: sex chromosome variability in Eigenmannia virescens (Gymnotiformes : Sternopygidae)

New data are presented on the sex chromosomes of the fish species Eigenmannia virescens (Gymnotiformes, Sternopygidae). A new finding, involving the occurrence of ZZ/ ZW sex chromosomes, is described in specimens sampled from the Sao Francisco and Amazon river basins in Brazil. All individuals had a chromosome number of 2n = 38. The homologs of the sex chromosome pair from the Sao Francisco river basin sample differed only in their morphology, while those from the Amazonian sample differed both in morphology and heterochromatin pattern. A possible model for the evolution of the sex chromosomes in E virescens is proposed, including data from populations from the Parana (Brazil) river basin, in which male heterogamety has already been described. The occurrence of different sex chromosome systems in species and populations of the neotropical freshwater fish fauna is discussed. Copyright (C) 2002 S. Karger AG, Basel.

Genetic monitoring of the Amazonian fish matrincha (Brycon cephalus) using RAPD markers: insights into supportive breeding and conservation programmes

The importance of genetic evaluations in aquaculture programmes has been increased significantly not only to improve effectiveness of hatchery production but also to maintain genetic diversity. In the present study, wild and captive populations of a commercially important neotropical freshwater fish, Brycon cephalus (Amazonian matrincha), were analyzed in order to evaluate the levels of genetic diversity in a breeding programme at a Brazilian research institute of tropical fish. Random Amplified Polymorphic DNA fingerprinting was used to access the genetic variability of a wild stock from the Amazon River and of three captive stocks that correspond to consecutive generations from the fishery culture. Although farmed stocks showed considerably lower genetic variation than the wild population, a significantly higher level of polymorphism was detected in the third hatchery generation. The results seem to reflect a common breeding practice on several hatchery fish programmes that use a small number of parents as broodstocks, obtaining reproductive success with few non-identified mating couples. The obtained data were useful for discussing suitable strategies for the genetic management and biodiversity conservation of this species.

A new species of Ctenobrycon Eigenmann, 1908 (Characiformes: Characidae) from the rio Orinoco basin, Venezuela

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Phylogenetic relationships within the speciose family Characidae (Teleostei: Ostariophysi: Characiformes) based on multilocus analysis and extensive ingroup sampling

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Registro de ocorrência de duas espécies de potamotrigonídeos na região do Alto Rio Paraná e algumas considerações sobre sua biologia

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Considerações sobre a reprodução de duas espécies de raias (Myliobatiformes, Potamotrygonidae) na região do Alto Rio Paraná, Sudeste do Brasil

This article presents preliminary information about the reproduction of two species of freshwater stingrays of the genus Potamotrygon, found in the Upper Parana River (Southwest Brazil), where these animals are exotic. Males of P. motoro and P. falkneri become sexually mature around 270 mm and 260 mm of disc width (DW), respectively. Females become sexually mature around 330 mm of DW in P. motoro and 325 mm in P. falkneri. In both species, females are bigger and heavier than males, reaching about 700 mm of DW and 20 kg. Copulating has not been recorded, but one courtship ritual has been observed during the dry season. Mature males present a different dentition from females and young males, which is probably related to their reproductive behavior instead of to their feeding habit. The female fertility has varied from one to three offspring. A miscarriage has been observed at different stages of embryonic development always when pregnant females were captured. In spite of the great history of anthropization of the study area, it seems that hydrological cycles are related to the reproduction of stingrays. Nevertheless, it would be necessary to conduct deeper studies to verify or not this influence.

Crescimento e mortalidade de Oligosarcus hepsetus (Cuvier, 1829) (Characiformes, Characidae) no Parque Estadual da Serra do Mar, Núcleo Santa Virgínia, São Paulo, Brasil

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Frugivory by the fish Brycon hilarii (Characidae) in western Brazil

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Increased B chromosome frequency and absence of drive in the fish Prochilodus lineatus

The neotropical freshwater fish species Prochilodus lineatus (Pisces, Prochilodontidae) shows 2n = 54 chromosomes plus supernumerary microchromosomes ranging in number from zero to seven among different animals. The transmission rates of B chromosomes were studied by the analysis of the parental and F1 generations in 10 controlled crosses performed with specimens from a natural population. The mean transmission rate observed for B chromosomes (k(B) = 0.511) was consistent with that expected from a regular meiotic behaviour orbs in both sexes and with the theoretical value under a Mendelian mode of transmission (0.5). Possible explanations for the dramatic increase in B frequency observed in this population during the last 10 years are discussed, bearing in mind the current absence of drive.

Animais aquáticos de importância médica no Brasil

The injuries caused by venomous and poisonous aquatic animals may provoke important morbidity in the victim. The cnidarians (jellyfishes, especially cubomedusas and Portuguese-Man-of-War) caused nearly 25% of 236 accidents by marine animals, while sea urchins were responsible for about 50% and catfish, stingrays and scorpionfish nearly 25%). In freshwater, stingrays and catfish cause injuries with a very similar mechanism to the poisoning and the effects of the toxins of marine species. In a series of about 200 injuries observed among freshwater fishermen, nearly 40% were caused by freshwater catfish, 5% freshwater stingrays and 55% by traumatogenic fish, such as piranhas and trairas. The author presents the aquatic animals that cause injuries to humans in Brazil, the clinical aspects of the envenoming and the first measures for the control of the severe pain observed mainly in the accidents caused by cnidarians and venomous fishes.

New records and distribution extensions of three species of Mesoclemmys Gray, 1863 (Testudines: Chelidae) in Mato Grosso state, Brazil, with observations on terrestrial movements

Distribution patterns of most of the 20 Neotropical freshwater turtles belonging to the family Chelidae are usually based on few locality records. We here report on vouchered records of three species of the chelid genus Mesoclemmys in the Brazilian state of Mato Grosso. Presence of M. vanderhaegei is confirmed for the Amazon River Basin, and M. gibba and M. raniceps are recorded in Mato Grosso for the first time. © 2012 Check List and Authors.