End4p/Sla2p Interacts with Actin-associated Proteins for Endocytosis in Saccharomyces cerevisiae

end4–1 was isolated as a temperature-sensitive endocytosis mutant. We cloned and sequenced END4 and found that it is identical to SLA2/MOP2. This gene is required for growth at high temperature, viability in the absence of Abp1p, polarization of the cortical actin cytoskeleton, and endocytosis. We used a mutational analysis of END4 to correlate in vivo functions with regions of End4p and we found that two regions of End4p participate in endocytosis but that the talin-like domain of End4p is dispensable. The N-terminal domain of End4p is required for growth at high temperature, endocytosis, and actin organization. A central coiled-coil domain of End4p is necessary for formation of a soluble sedimentable complex. Furthermore, this domain has an endocytic function that is redundant with the function(s) of ABP1 and SRV2. The endocytic function of Abp1p depends on its SH3 domain. In addition we have isolated a recessive negative allele of SRV2 that is defective for endocytosis. Combined biochemical, functional, and genetic analysis lead us to propose that End4p may mediate endocytosis through interaction with other actin-associated proteins, perhaps Rvs167p, a protein essential for endocytosis.

The Michigan Historical Collections ... some impressions.

Mode of access: Internet.

Wrinkles and recipes /

Mode of access: Internet.

The library in the TVA adult education program,

"Selected references": p. [67]-68.

Experimental science : elementary practical and experimental physics.

Mode of access: Internet.

Scientific American

Mode of access: Internet.

Scientific American.

Called "Building edition" beginning with v. 19, no. 1 (Jan. 1895).

Principles of general ecology.

Includes bibliography.

The American orator : with an appendix containing the Declaration of Independence, with the fac-similes of the autographs of the signers; the Constitution of the United States; Washington's Farewell Address; and fac-similes of the autographs of a large number of distinguished individuals /

Mode of access: Internet.

The practical land drainer : A treatise on draining land. In which the most approved systems of drainage and scientific principles on which they depend, are explained, and their comparative merits discussed ... Numerously illustrated /

Mode of access: Internet.

The finger print instructor,

Mode of access: Internet.

The wisdom of Doctor Dodypoll.

One copy kept in Munn Collection.

The wit of a woman.

One copy kept in Munn Collection.

The three lords and three ladies of London /

One copy kept in Munn Collection.