Evalution of methods for the detection of MRSA in patients of Botucatu Medical School Hospital, São Paulo, Brazil

Oxacillin is the main drug of choice for the treatment of S. aureus infections. However, S. aureus resistance to oxacillin has become a major problem in the recent decades. The study aimed assess the rates of oxacillin resistance in S. aureus samples obtained at the Botucatu Medical School Hospital, UNESP, and to compare phenotypic techniques for the detection of MRSA against the gold standard method (mecA gene detection) in these samples. A total of 102 samples, previously isolated between 2002 and 2006, and kept at the Culture Collection of the Department of Microbiology and Immunology, in the Botucatu Biosciences Institute, UNESP, were included. Oxacillin resistance was assessed by oxacillin and cefoxitin disk diffusion and agar dilution tests, screening tests using Mueller-Hinton agar with 6 mu g/mL of oxacillin and 4% NaCl, E-test, and mecA gene detection. of the samples analyzed, 46 (45.1%) were mecA-positive. Oxacillin disk sensitivity and specificity were 86.9% and 91.1%, respectively. Cefoxitin disk sensitivity and specificity were respectively 91.3% and 91.1%. The screening test with the cefoxitin disk showed almost the same level of sensitivity (91.3%) and specificity (91.1%). With E-test strips, sensitivity was higher (97.8%) and specificity was comparable to that found with the other methods (91.1%). Ninety-three percent of the samples produced beta-lactamase and five of them were mecA-negative. There was a gradual increase in the number of oxacillin-resistant S. aureus samples between 2002 and 2004. However, from 2004 to 2006, the number of resistant samples dropped from 55% of MRSA in 2004, to 45% in 2005 and 34.6% in 2006. The data obtained reveal that, among phenotypic methods, the E-test yielded the best results, with higher sensitivity levels when compared to the other methods. The decreased resistance rate observed over the most recent years may be explained by the rational use of antimicrobial agents associated with good practices in the control of hospital infection, or may be related to the diminished use of oxacillin as a treatment option.

Seasonal population dynamics and the genetic structure of the mosquito vector Aedes aegypti in São Paulo, Brazil

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Prevalence of dog intestinal parasites and risk perception of zoonotic infection by dog owners in São Paulo State, Brazil

Coprological examination was used to estimate the prevalence of gastrointestinal parasites in stray and domiciled dogs from Botucatu, São Paulo State, Brazil. Risk factors for dog infection were assessed in relation to demographic, husbandry and management data. The dog owners completed a questionnaire survey on some aspects of dog parasitism such as parasite species, mechanisms of infection, awareness of zoonotic diseases and history of anthelmintic usage. Parasites were found in the faeces of 138 dogs, with an overall prevalence of 54.3%. Dogs harbouring one parasite were more common (31.4%) than those harbouring two (18.5%), three (3.2%) or four (1.2%). The following parasites and their respective frequencies were detected: Ancylostoma (37.8%), Giardia (16.9%), Toxocara canis (8.7%), Trichuris vulpis (7.1%), Dipylidium caninum (2.4%), Isospora (3.5%), Cryptosporidium (3.1%) and Sarcocystis (2.7%). Stray dogs were found more likely to be poliparasitized (P < 0.01) and presented higher prevalence of Ancylostoma, T. canis and Giardia (P < 0.01) than domiciled ones. Toxocara canis was detected more frequently in dogs with < 6 months of age (P < 0.05) and no effect of sex or breed could be observed (P > 0.05). Except for Ancylostoma, that showed a significantly higher prevalence in dogs living in a multi-dog household (P < 0.01), parasite prevalences were similar in single- and multi-dog household. The answers of dog owners to the questionnaire showed that the majority does not know the species of dog intestinal parasites, the mechanisms of transmission, the risk factors for zoonotic infections, and specific prophylactic measures. The predominance of zoonotic species in dogs in the studied region, associated with the elevated degree of misinformation of the owners, indicates that the risk of zoonotic infection by canine intestinal parasite may be high, even in one of the most developed regions of Brazil.

Anurans of a riparian forest in São Carlos, state of São Paulo, Brazil

No presente estudo foi realizado um inventário das espécies de anfíbios anuros que ocorrem em um fragmento de mata ciliar do Córrego do Espraiado, localizado na Universidade Federal de São Carlos (UFSCar), Município de São Carlos, estado de São Paulo, sudeste do Brasil. Durante os meses de março de 2009 a fevereiro de 2010 foram realizadas saídas de campo semanais nas quais foram registradas 13 espécies de anuros. A atividade reprodutiva das espécies de anuros componentes da comunidade estudada mostrou-se sazonal, sendo que houve correlação positiva e significativa entre o número de espécies em atividade reprodutiva e o fotoperíodo. Verificamos que a comunidade estudada mostrou-se mais semelhante a comunidades de anuros de florestas de transição entre Mata Atlântica e Cerrado do que a fragmentos de Cerrado próximos da região.

Social Wasp Diversity (Vespidae, Polistinae) in a Forest Fragment in the Northeast of São Paulo State Sampled with Different Methodologies

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Identification of Landslide Scars in the Region of the Serra do Mar, São Paulo State, Brazil, Using Digital Image Processing and Spatial Analysis Tools

The objective of the present study, developed in a mountainous region in Brazil where many landslides occur, is to present a method for detecting landslide scars that couples image processing techniques with spatial analysis tools. An IKONOS image was initially segmented, and then classified through a Batthacharrya classifier, with an acceptance limit of 99%, resulting in 216 polygons identified with a spectral response similar to landslide scars. After making use of some spatial analysis tools that took into account a susceptibility map, a map of local drainage channels and highways, and the maximum expected size of scars in the study area, some features misinterpreted as scars were excluded. The 43 resulting features were then compared with visually interpreted landslide scars and field observations. The proposed method can be reproduced and enhanced by adding filtering criteria and was able to find new scars on the image, with a final error rate of 2.3%.

Sedimentation of the Cretaceous Bauru Group in São Paulo, Parana Basin, Brazil

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Fish-assemblage structure of the Corumbatai river basin, São Paulo State, Brazil: characterization and anthropogenic disturbances

O rio Corumbataí é um dos principais tributários da margem direita do rio Piracicaba que é um tributário do rio Tietê. O rio Corumbataí integra a bacia do rio Paraná e é regionalmente importante não só por possuir águas de boa qualidade, mas também por possuir elementos raros na paisagem local. Este estudo visou caracterizar as assembléias de peixes do rio Corumbataí e fornecer dados que contribuam para uma avaliação da sua qualidade ambiental. Na bacia do rio Corumbataí, foram amostrados 4 rios principais, cada um com 3 pontos de coleta. Vinte e quatro amostras foram coletadas durante os meses de março a julho e de setembro a dezembro de 2001. Dados bióticos foram avaliados por medidas de diversidade. Um modelo linear ANCOVA foi utilizado para testar a hipótese de variação espaço-temporal nas assembléias de peixes, com a riqueza de espécies como variável resposta, ordem do rio como fator e temperatura e logaritmo natural do número de indivíduos como covariáveis. Esta análise mostrou uma variação espaço-temporal que é corroborada por conceitos exaustivamente discutidos na literatura, tais como relação espécie-área e o conceito de rio contínuo. Dados provenientes do rio Ribeirão Claro mostraram um padrão diferente quando comparados com os outros rios. Esta diferença foi provavelmente devido à interferência humana e atesta o processo de fragmentação de hábitats aquáticos que podem ter levado a um isolamento das populações locais de peixes.

Migration of the curimbatá Prochilodus lineatus (Valenciennes, 1836) (Pisces, Prochilodontidae) at the waterfall Cachoeira de Emas of the Mogi-Guaçu river - São Paulo, Brazil

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

First record of the ghost bat Diclidurus scutatus Peters (Mammalia, Chiroptera, Emballonuridae) in São Paulo city, Brazil

Devido aos seus hábitos de voar alto e se abrigar em folhas de palmeiras, há poucos registros na literatura sobre o morcego Diclidurus scutatus Peters, 1869. No Brasil, essa espécie é conhecida somente na região Norte e o presente estudo descreve sua primeira ocorrência na região Sudeste. O morcego foi encontrado morto no parapeito da janela de um apartamento do nono andar, em um edifício residencial, na área urbana da cidade de São Paulo, estado de São Paulo. Provavelmente esse morcego devia viver na Serra da Cantareira, um fragmento da mata Atlântica, próximo da cidade de São Paulo.

Incidence of self-reported hearing loss and associated risk factors among the elderly in São Paulo, Brazil: the SABE survey

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Influence of biosolids rate on chemical properties of an oxisol in São Paulo, Brazil

Organic residues may cause major health and environmental problems. This is the case in our study area, where more than 10 billion L per year of residential and industrial waste are produced. Land application of biosolids can be an economical solution by recycling waste and can provide valuable fertilizer if used correctly. The aim of this work was to study the effect of biosolids on the chemical properties of an Oxisol. The experiment was located at Ilha Solteira northwest of São Paulo State, Brazil. The soil was cropped to Sorghum bicolor.The field experimental design consisted of random blocks with six treatments and four replications of each treatment. Biosolids were surface applied to four treatments at rates of 5, 10, 20, and 40 Mg ha(-1) on a dry matter basis; in addition, a treatment with mineral fertilizer and a control were included. One year after biosolids application, soil samples were taken at 0-10, 10-20, and 20-40 cm. Organic matter content (Walkley-Black) and pH (CaCl2) were routinely determined. Cation exchange capacity, exchangeable bases (Ca, Mg, K), and P were determined by exchange resin extraction. No significant differences in any of the analyzed properties were found below the 20 cm depth. Extractable phosphorus (P) and potassium (K) increased with increasing biosolids rate in the top 20 cm, whereas calcium (Ca) and (Ma) magnesium content were not significantly influenced by biosolids. Soil pH decreased with increasing biosolids application. The sewage sludge application did not influence the sorghum production in the first year of culture, under unfavorable soil moisture conditions, but it influenced the dry matter.

Influence of water level variation on biomass and chemical composition of the aquatic macrophyte Eichhornia azurea (Kunth) in an oxbow lake of the Rio Mogi-Guacu (São Paulo, Brazil)

The influence of water level variation (flood pulse) on the biomass and chemical composition of the aquatic macrophyte Eichhornia azurea, was investigated in a tropical oxbow lake of the Rio Mogi-Guacu, State of São Paulo, Brazil. The flood pulse causes an increase in total nitrogen content from 0.67 to 1.35 mg/L and total phosphorus content from 10.5 to 101.0 mu g/L of the water. This fertilization, associated with other factors, determines a typical seasonal variation in the biomass and chemical composition of the macrophyte.

Potential of municipal solid waste (MSW) as a source of energy in São Paulo: Its impact on CO2 balance

Energy generation is needed in São Paulo and MSW represents a promising alternative, although it is more expensive than hydroelectric power. About 14 900 t/day of MSW is generated, of which 8433 t/day is domestic and commercial MSW. From this amount, 1800 t will be destined to generate 30 MW of power. The eco-balance of CO2 has been considered for incineration and recycling. The recycling program of plastics, metals, paper and glass would represent a significant reduction in energy and CO2 emission. The total CO2 released is 3.34 x 10(5) t/yr without recycling. and is 1.25 x 10(5) t/yr with a recycling program. Most of the CO2 comes from plastics and paper production. Economic aspects could probably favor Incineration with energy production as the best option. (C) 1998 Published by Elsevier B.V. Ltd. All rights reserved.