Behavior of Peruvian carrot (Arracacia xanthorrhiza) and cassava (Manihot esculenta) starches subjected to heat-moisture treatment

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Antioxidant activity and determination of total phenol contents, carotenoids, beta-carotene, lycopene and zinc in white, yellow and pinkish varieties of manihot esculenta crantz

There are cassava varieties that present compounds as carotenoids, beta-carotene, lycopene and minerals important for human and animal health. The present study evaluated the antioxidant activity of the white, yellow and pinkish varieties of Manihot esculenta, by mean of the DPPH test and by the ferrous ion-chelating activity. Furthermore, the total phenols, carotenoids, beta-carotene, lycopene and zinc contents were also determined. Utilizing the DPPH test it was possible to find that extracts of boiled samples presented higher antioxidant activity (89.53% -pinkish) in comparison to the fresh samples (1.97% -white). For the ferrous ion-chelating test, the highest activity was found for the boiled pinkish variety extract (63.43%) and the lowest was for fresh yellow extract (17.34%) the white sample did not present activity. The highest concentration of total phenols and zinc content was obtained for the boiled pinkish variety extract 136.12 mg EAG/g of extract and 0,811ppm, respectively, in the concentration of 1000 mu g/mL. The pinkish variety presented also higher quantity of pigments, including carotenoid (29.40 mu g/g), beta-carotene (9.14 mu g/100g) and lycopene (68.92%). According to the results obtained in this study it was possible to conclude that the yellow and pinkish varieties of M. esculenta present quantity of phenolic compounds and minerals sufficient to attribute the antioxidant activity and may thus contribute to reduce oxidative damage and be used as nutraceuticals or directly ingested in the diet to maintain good health.

Behavior of Peruvian carrot (Arracacia xanthorrhiza) and cassava (Manihot esculenta) starches subjected to the heat-moisture treatment

Peruvian carrot and cassava starches were isolated, adjusted to 30 and 35% moisture, and heatedat 90°C for 8 h. Structural and physicochemical characteristics of the treated starches wereevaluated and compared. High performance anion exchange chromatography with pulsedamperometric detector (HPAEC-PAD), gel permeation chromatography (GPC), and amylosecontent, revealed that the HMT did not change the chemical structures of the starches. A largeagglomeration of granules was observed from SEM, particularly in the Peruvian carrot starch.Crystalline patterns in Peruvian carrot and cassava starches changed from B to C and CAto A,respectively. Relative crystallinity decreased from 30 to 25% in Peruvian Carrot starch, andincreased from 35 to 37% in cassava starch adjusted to 30% moisture. SF and peak viscositydecreased, breakdown was almost completely eliminated (particularly in the Peruvian carrotstarch), and final viscosity increased. WAI and WSI increased as moisture levels of bothstarches increased. Gelatinization temperatures increased and enthalpy decreased. Degrees ofgelatinization increased as the moisture level increased, reaching 33 and 72% in the cassavaand Peruvian carrot starches, respectively. HMT strengthened the intra- and intermolecularinteractions of starches and increased their stability during heating and shearing, but also causeda partial gelatinization in the starches, particularly in Peruvian carrot starch.

Atividade antioxidante e determinação de fenóis totais, carotenoides, betacarotenos, licopeno e zinco em variedades branca, amarela e rosada de Manihot Esculenta Crantz

There are cassava varieties that present compounds as carotenoids, beta-carotene, lycopene and minerals important for human and animal health. The present study evaluated the antioxidant activity of the white, yellow and pinkish varieties of Manihot esculenta, by mean of the DPPH test and by the ferrous ion-chelating activity. Furthermore, the total phenols, carotenoids, beta-carotene, lycopene and zinc contents were also determined. Utilizing the DPPH test it was possible to find that extracts of boiled samples presented higher antioxidant activity (89.53% - pinkish) in comparison to the fresh samples (1.97% - white). For the ferrous ion-chelating test, the highest activity was found for the boiled pinkish variety extract (63.43%) and the lowest was for fresh yellow extract (17.34%) the white sample did not present activity. The highest concentration of total phenols and zinc content was obtained for the boiled pinkish variety extract 136.12 mg EAG/g of extract and 0,811ppm, respectively, in the concentration of 1000 µg/mL. The pinkish variety presented also higher quantity of pigments, including carotenoid (29.40 µg/g), beta-carotene (9.14 µg/100g) and lycopene (68.92%). According to the results obtained in this study it was possible to conclude that the yellow and pinkish varieties of M. esculenta present quantity of phenolic compounds and minerals sufficient to attribute the antioxidant activity and may thus contribute to reduce oxidative damage and be used as nutraceuticals or directly ingested in the diet to maintain good health.

Structural insights regarding an insecticidal Talisia esculenta protein and its biotechnological potential for Diatraea saccharalis larval control

Talisin is a seed-storage protein from Talisia esculenta that presents lectin-like activities, as well as proteinase-inhibitor properties. The present study aims to provide new in vitro and in silico biochemical information about this protein, shedding some light on its mechanistic inhibitory strategies. A theoretical three-dimensional structure of Talisin bound to trypsin was constructed in order to determine the relative interaction mode. Since the structure of non-competitive inhibition has not been elucidated, Talisin-trypsin docking was carried out using Hex v5.1, since the structure of non-competitive inhibition has not been elucidated. The predicted non-coincidence of the trypsin binding site is completely different from that previously proposed for Kunitz-type inhibitors, which demonstrate a substitution of an Arg(64) for the Glu(64) residue. Data, therefore, provide more information regarding the mechanisms of non-competitive plant proteinase inhibitors. Bioassays with Talisin also presented a strong insecticide effect on the larval development of Diatraea saccharalis, demonstrating LD50 and ED50 of ca. 2.0% and 1.5%, respectively. (C) 2011 Elsevier Inc. All rights reserved.

Fenología de los estados vegetativos de mandioca (Manihot esculenta Crantz) en base al tiempo térmico

La mandioca (Manihot esculenta Crantz) constituye la cuarta fuente mundial de calorías en la alimentación humana, es apta para la nutrición animal y para extracción de biocombustible. Este artículo tiene como objetivo evaluar el comportamiento de un modelo de tiempo térmico (TT) para caracterizar las fases fenológicas (FF) de dos cultivares de mandioca en un ciclo de crecimiento de 280 días, cultivados bajo condiciones de campo en Corrientes, Argentina. Las observaciones se realizaron durante las campañas 2007/2008 y 2008/2009. El cálculo del TT se basó en el método residual, la temperatura base fue 16°C. Entre ambos cultivares se observaron diferencias en los grados-día (GD) acumulados para cumplir las FF de expansión de la primera (00-01) y novena hoja (00-02 H9), y en la de engrosamiento de raíces (00-04 ERR), esta última relacionada con el índice de área foliar (IAF). Para completar el ciclo de crecimiento los cultivares Palomita y Amarilla requirieron entre 2027 y 2096 GD, respectivamente. El patrón de crecimiento y desarrollo fenológico de los cultivares de mandioca basado en los GD acumulados, puede utilizarse para caracterizar el progreso del cultivo en el bioambiente de Corrientes.

Increased CO2 modifies the carbon balance and the photosynthetic yield of two common Arctic brown seaweeds: Desmarestia aculeata and Alaria esculenta

Ocean acidification affects with special intensity Arctic ecosystems, being marine photosynthetic organisms a primary target, although the consequences of this process in the carbon fluxes of Arctic algae are still unknown. The alteration of the cellular carbon balance due to physiological acclimation to an increased CO2 concentration (1300 ppm) in the common Arctic brown seaweeds Desmarestia aculeata and Alaria esculenta from Kongsfjorden (Svalbard) was analysed. Growth rate of D. aculeata was negatively affected by CO2 enrichment, while A. esculenta was positively affected, as a result of a different reorganization of the cellular carbon budget in both species. Desmarestia aculeata showed increased respiration, enhanced accumulation of storage biomolecules and elevated release of dissolved organic carbon, whereas A. esculenta showed decreased respiration and lower accumulation of storage biomolecules. Gross photosynthesis (measured both as O2 evolution and 14C fixation) was not affected in any of them, suggesting that photosynthesis was already saturated at normal CO2 conditions and did not participate in the acclimation response. However, electron transport rate changed in both species in opposite directions, indicating different energy requirements between treatments and species specificity. High CO2 levels also affected the N-metabolism, and 13C isotopic discrimination values from algal tissue pointed to a deactivation of carbon concentrating mechanisms. Since increased CO2 has the potential to modify physiological mechanisms in different ways in the species studied, it is expected that this may lead to changes in the Arctic seaweed community, which may propagate to the rest of the food web.

Estudio Energético y de Explotación de diferentes conjuntos de Labranza para el Cultivo de la Yuca (Manihot esculenta, Crantz) en un Fluvisol

El trabajo se desarrolló en tres etapas de investigación, las dos primeras fueron realizadas en la Unidad Básica de Producción Cooperativa “El Palmar” perteneciente a la Empresa de Cultivos Varios "Paquito Rosales Benítez" del municipio de Yara, provincia de Granma, Cuba, el cual se llevó a cabo en el período de abril de 2005 a enero de 2008; y de noviembre de 2012 a febrero de 2013 respectivamente; la tercera se ejecutó de mayo a junio de 2013 en un canal de suelo ubicado en la nave de maquinaria del Departamento de Ingeniería Rural de la Universidad Politécnica de Madrid. En la primera etapa se evaluaron los conjuntos tractor-apero que intervienen en la realización de cuatro sistemas de labranza para el cultivo de la yuca, variedad Enana Rosada. Los sistemas de laboreo fueron: T1, labor de aradura (alzado) con un arado de discos seguidas de un pase de grada de discos un pase cruzado de un escarificador y un pase con una grada de discos (5 labores); T2, laboreo mínimo que comprende una labor profunda con un escarificador con saetas seguido por una labor de grada de discos (2 labores); T3, laboreo mínimo en el que se realizaron dos pases cruzados con escarificador y una labor de fresado con una fresadora de eje horizontal (3 labores) y; T4, dos labores cruzadas con la grada de discos seguidas por otras dos pases cruzados con el escarificador y una última labor con la grada de discos (5 labores). Se realizaron cinco observaciones de la jornada laboral para cada uno de los conjuntos tractorapero de cada sistema de laboreo en tres campañas, evaluando el consumo de combustible, las diferentes productividades, los índices y coeficientes tecnológicos y de explotación, el rendimiento del cultivo el balance energético del mismo, y la emisión de CO2 equivalente. En la segunda etapa se determinó la fuerza de tiro requerida por un órgano de trabajo de un escarificador con saetas en condiciones de campo, en tres profundidades (15, 25 y 35 cm) y a cuatro velocidades de trabajo (1,30; 1,49; 1,65 y 2,32 m s-1). En la tercera etapa se determinaron las fuerzas horizontales y verticales en una maqueta experimental de un órgano de trabajo de un escarificador con saetas (1:5 del tamaño real del apero) en cuatro contenidos de humedad (60, 90, 120 y 150 g kg-1), tres densidades del suelo (1,1; 1,2 y 1,3 Mg m-3) y una velocidad de 40 mm s-1. Se comparan los resultados del apero a tamaño real y la maqueta experimental. Los mejores resultados relacionados con los indicadores evaluados a la maquinaria correspondieron al sistema de laboreo mínimo T2, el cual presenta la mejor productividad por hora de tiempo limpio (1,34 ha h-1) y de tiempo operativo (1,27 ha h-1), con diferencias significativas respecto al resto de los sistemas de labranza evaluados; así como el menor consumo de combustible (32,4 L ha-1) y el mejor comportamiento energético, con diferencias significativas respecto a las demás, resultando inferior en un 63, 30 y 58 % con relación a T1, T3 y T4. La fuerza de tiro del órgano de trabajo del escarificador con saetas se incrementa con la densidad del suelo y con la profundidad, y velocidad de trabajo. Las fuerzas horizontales y verticales de la maqueta se incrementan linealmente con la densidad y humedad del suelo. Existe una relación lineal entre las fuerzas de tiro y horizontales medidas en el apero real y en el apero a escala con el contenido de humedad del suelo. ABSTRACT The work was developed in three stages of research, the first two were conducted in the Basic Unit of Cooperative Production "El Palmar" belonging to the agricultural farm "Paquito Rosales Benitez" in the municipality of Yara, Granma Province, Cuba, the which was conducted in the period from April 2005 to January 2008 and November 2012 and February 2013 respectively, the third from May to June 2013 on a soilbin located in the ship's machinery of Rural Engineering Department of the Technical University of Madrid. In the first stage were evaluated tractor-implement sets involved in the completion of four tillage systems for cassava, Pink Dwarf variety. Tillage systems were: T1, plowing (elevation) with a disk plow followed by a pass of disc harrow, a cross of scarifier and a pass with a disc harrow (5 labours), T2, minimum tillage comprising a deep work with a scarifier with a bolt followed by a disc harrow work (two labours); T3, minimum tillage which two crossed passes performed with scarifier and milling labour with a horizontal axis milling machine (three labours) and, T4, two crossed labours with disc harrow followed by two crossed passes with the scarifier and a final labour with disc harrow (5 labours). Five observations of the working day for each tractor-implement sets of each tillage system in three seasons were performed, evaluating fuel consumption, different productivities, indexes and technological and operational factors, the crop yield energy balance and the emission of CO2 equivalent. In the second stage we determined the draft force required by a working body of a scarifier with bolt under field conditions at three depths (15, 25 and 35 cm) and four operating speeds (1.30, 1.49 , 1.65 and 2.32 m s-1). In the third stage were determined horizontal and vertical forces on an experimental model of a working body of a scarifier with bolt (1:5 real scale of the implement) in four moisture contents (60, 90, 120 and 150 g kg -1), three soil densities (1.1, 1.2, and 1.3 Mg m-3) and a speed of 40 mm s-1.The results of real scale implement the experimental model were compared. The best results related to evaluated machinery indicators corresponded to T2 minimum tillage system, which presents the best productivity per hour clean time (1.34 ha h-1) and operating time (1.27 ha h-1), with significant differences compared to other tillage systems evaluated; as well as lower fuel consumption (32.4 L ha-1) and the best energy performance, with significant differences from the other, resulting lower by 63, 30 and 58% compared to T1, T3 and T4. The draft force of the working body of the scarifier with bolt increases with soil density and depth, and speed of work. The horizontal and vertical forces of the model increase linearly with density and soil moisture. A linear relationship exists between the shot and horizontal forces measured at the real implement and scale implement with the soil moisture content.

A short note on starch and xylem of Colocasia esculenta (taro) in archaeological deposits from Pitcairn Island, southeast Polynesia

We present results of starch analysis of archaeological deposits from Pitcairn Island. High concentrations of starch grains preserved in cell membranes, and xylem tracheary elements, consistent with introduced Colocasia esculenta (taro) were found. Because of limited age control, we are uncertain if the microfossils are prehistoric. Problems associated with identifying taxa with small starch grains in extractions from weathered deposits are highlighted. (c) 2006 Elsevier Ltd. All rights reserved.

Early and mid Holocene tool-use and processing of taro (Colocasia esculenta), yam (Dioscorea sp.) and other plants at Kuk Swamp in the highlands of Papua New Guinea

Recent multidisciplinary investigations document an independent emergence of agriculture at Kuk Swamp in the highlands of Papua New Guinea. In this paper we report preliminary usewear analysis and details of prehistoric use of stone tools for processing starchy food and other plants at Kuk Swamp. Morphological diagnostics for starch granules are reported for two potentially significant economic species, taro (Colocasia esculenta) and yam (Dioscorea sp.), following comparisons between prehistoric and botanical reference specimens. Usewear and residue analyses of starch granules indicate that both these species were processed on the wetland margin during the early and mid Holocene. We argue that processing of taro and yam commences by at least 10,200 calibrated years before present (cal BP), although the taro and yam starch granules do not permit us to distinguish between wild or cultivated forms. From at least 6950 to 6440 cal BP the processing of taro, yam and other plants indicates that they are likely to have been integrated into cultivation practices on the wetland edge.