262 resultados para Lages nervuradas
Resumo:
A Work Project, presented as part of the requirements for the Award of a Masters Degree in Management from the NOVA School of Business and Economics
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O mtodo imunocitoqumico de peroxidase anti-peroxdase aplicado, pela primeira vez, em tecidos de natimorto macerado de II-III graus para colorao especfica de amastigotas do Trypanosoma cruzi, em caso de doena de Chagas congnita. Os resultados mostram que o encontro de ninhos de amastigotas muito facilitado pelo mtodo, mesmo em tecidos autolisados, onde a morfologia dos parasitas geralmente se mostra muito alterada; o mtodo recomendvel, tambm nestes casos, para a avaliao mais precisa da intensidade do parasitismo.
Resumo:
A Work Project, presented as part of the requirements for the Award of a Masters Degree in Management from the NOVA School of Business and Economics
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A Work Project, presented as part of the requirements for the Award of a Masters Degree in Management from the NOVA School of Business and Economics
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This research characterizes the acute and chronic phases of Chagas ' disease in hamster through parasitological and histopathological studies. The acute phase was achieved with 44 young hamsters injected intraperitoneally with 100.000 blood trypomastigotes of Benedito and Y strains of T. cruzi. The chronic phase was induced in 46 hamsters injected intraperitoneally with 35.000 trypomastigotes ofVicentina, Benedito and Y strains. Animals were sacrificed at regular intervals of 24 hours of acute phase and from the 3rd to the 10th month of infection ofchronic phase. In the acute phase, parasites were easily recoveredfrom all animals and there was an inflammatory reaction characterized by mononuclear and polymorphous leukocyte infiltration of variable degree in the majority of tissues and organs, specially in the connective loose and fatty tissues, smooth muscle myocardium and skeletal muscle. In the chronic phase the lesions occurred in the same tissues and organs, but the inflammatory response was less severe and characterized by mononuclear infiltration mainly with focal or zonalfibrosis in the myocardiun. In 50% of infected animals parasites were found inmyocardiun and recoveredfrom pericardic, peritoneal and ascitic fluids in some animals. Signs of heart failure, sudden death and enlargement of bowel were observed regularly. We concluded that the hamster is an useful model for Chagas' disease studies.
Resumo:
Informa-se, pela primeira vez, os achados de Trypanosoma rangeli no Tringulo Mineiro, Sudeste do Brasil, rea altamente endmica para doena de Chagas, assim como a infeco natural da espcie Didelphis albiventris.com este mesmo tripanosoma. Estes foram demonstrados por esfregaos sangneos, xenodiagnstico, hemocultura, microhematcrito e PCR. A PCR foi realizada nas fezes e hemolinfa de Triatoma infestans, usando como controle cepas de T. rangeli provenientes da Colmbia.
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Com o objetivo de se obter um modelo experimental que permitisse estabelecer a despopulao (desnervao) neuronal cardaca procurou-se pesquisar o comportamento do sistema nervoso intracardaco em hamsters cronicamente infectados com o T. cruzi. Para tal fim, realizaram-se contagens dos neurnios do plexo nervoso autonmico intracardaco em hamsters inoculados com 35.000 formas sangneas de trs cepas diferentes, sacrificados 5, 8 e 10 meses depois da infeco. Demonstrou-se, pela primeira vez, destruio neuronal significativa num modelo experimental, similar que ocorre na doena de Chagas humana.
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White cell (WBC)-reduction filters have been shown to be effective in removing infectious agents from infected blood products. In this study, the mechanisms of Trypanosoma cruzi (T. cruzi) retention by WBC-reduction filters were assessed. Human packed red blood cell (PRBC) and platelet concentrate (PC) samples were contaminated with T. cruzi organisms (Y strain; 3.4 x 10(6)/ml), and then filtered using WBC-reduction experimental filters that provided about 3 log10 WBC removal. Transmission electron microscopy sections showed that T. cruzi parasites were removed from contaminated PRBC and PC samples primarily by mechanical mechanism without interacting with filter fibbers or blood cells. In addition, we found that T. cruzi parasites were also removed by a direct fibber adhesion. These data indicate that T. cruzi parasites are removed from infected blood not only by mechanical mechanism but also by biological mechanism probably mediated by parasite surface proteins.
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Beginning the study of chronic pathologic changes in pancreas of hamsters experimentally infected with Trypanosoma cruzi Vic strain, hepatocyte metaplasia was observed in one animal from infected group. This is the first report of oncocytes in Chagas' disease, which could be due to aberrant regenerative response to pancreas inflammatory process.
Resumo:
A Work Project, presented as part of the requirements for the Award of a Masters Degree in Management from the NOVA School of Business and Economics
Resumo:
A Work Project, presented as part of the requirements for the Award of a Masters Degree in Management from the NOVA School of Business and Economics
Resumo:
A Work Project, presented as part of the requirements for the Award of a Masters Degree in Management from the NOVA School of Business and Economics
Resumo:
A Work Project, presented as part of the requirements for the Award of a Masters Degree in Management from the NOVA School of Business and Economics
