Generation of TALEN-mediated GRdim knock-in rats by homologous recombination.

Transcription Activator-Like Effector Nucleases (TALEN) are potential tools for precise genome engineering of laboratory animals. We report the first targeted genomic integration in the rat using TALENs (Transcription Activator-Like Effector Nucleases) by homology-derived recombination (HDR). We assembled TALENs and designed a linear donor insert targeting a pA476T mutation in the rat Glucocorticoid Receptor (Nr3c1) namely GR(dim), that prevents receptor homodimerization in the mouse. TALEN mRNA and linear double-stranded donor were microinjected into rat one-cell embryos. Overall, we observed targeted genomic modifications in 17% of the offspring, indicating high TALEN cutting efficiency in rat zygotes.

Catalogue sommaire des manuscrits indiens, indo-chinois et malayo-polynésiens de la Bibliothèque nationale

Contient les descriptions des manuscrits Indien 1 à 1064, les descriptions des manuscrits indo-chinois 1 à 512, les additions et corrections par Petrus Voorhoeve (1899-1996), 1952, des notices du catalogue sommaire des manuscrits malayo-polynésiens de la Bibliothèque nationale par A. Cabaton, Paris 1912, les descriptions des manuscrits malayo-polynésiens 1-244 par A. Cabaton et celles des manuscrits malayo-polynésiens 245-283, rédigées par Petrus Voorhoeve (1899-1996),