Alpha-helical, but not beta-sheet, propensity of proline is determined by peptide environment.

Proline is established as a potent breaker of both alpha-helical and beta-sheet structures in soluble (globular) proteins. Thus, the frequent occurrence of the Pro residue in the putative transmembrane helices of integral membrane proteins, particularly transport proteins, presents a structural dilemma. We propose that this phenomenon results from the fact that the structural propensity of a given amino acid may be altered to conform to changes imposed by molecular environment. To test this hypothesis on proline, we synthesized model peptides of generic sequence H2N-(Ser-LyS)2-Ala- Leu-Z-Ala-Leu-Z-Trp-Ala-Leu-Z-(Lys-Ser)3-OH (Z = Ala and/or Pro). Peptide conformations were analyzed by circular dichroism spectroscopy in aqueous buffer, SDS, lysophosphatidylglycerol micelles, and organic solvents (methanol, trifluoroethanol, and 2-propanol). The helical propensity of Pro was found to be greatly enhanced in the membrane-mimetic environments of both lipid micelles and organic solvents. Proline was found to stabilize the alpha-helical conformation relative to Ala at elevated temperatures in 2-propanol, an observation that argues against the doctrine that Pro is the most potent alpha-helix breaker as established in aqueous media. Parallel studies in deoxycholate micelles of the temperature-induced conformational transitions of the single-spanning membrane bacteriophage IKe major coat protein, in which the Pro-containing wild type was compared with Pro30 --> Ala mutant, Pro was found to protect the helix, but disrupt the beta-sheet structure as effectively as it does to model peptides in water. The intrinsic capacity of Pro to disrupt beta-sheets was further reflected in a survey of porins where Pro was found to be selectively excluded from the core of membrane-spanning beta-sheet barrels. The overall data provide a rationale for predicting and understanding the structural consequences when Pro occurs in the context of a membrane.

University of Michigan Men's Basketball Team, 1982

Back Row: asst. coach Don Sicko, trainer Mark Healy, Tim McCormick, Dan Pelekoudas, Leslie Rockymore, coach Bill Frieder, Ike Person, Greg Washington? , asst. Mike Boyd, equip. mngr. Bob Hurst

Front Row: mngr. William Mitchell?, Willis Carter, Eric Turner, Dean Hopson, captain Thad Garner, Memi C. Burton, Leo Burton, Jon Antonides, asst. coach Edwin VanDeWege

University of Michigan Men's Basketball Team, 1983

Top Row: trainer Mark Healy, grad asst. Ernie Sellers, asst. coach Mike Boyd, head coach Bill Frieder, aast. coach Bud VanDeWege, mngr. Bill Mitchell

Middle Row: equip. mngr. Bob Hurst, Paul Jokisch, Robert Henderson, Roy Tarpley, co-captain Ike Person, co-captain Leo Brown, Tim McCormick, Butch Wade, Jon Antonides

Front Row: mngr. Bill Fleming, Ron Gibas, Richard Rellford, Dan Pelekoudas, Eric Turner, Leslie Rockymore, Robert McFarland, Gerard Rudy, Phil Giroux

University of Michigan Men's Basketball Team, 1983

Standing: Ike Person, Richard Rellford, Paul Jokisch, Robert Henderson, Roy Tarpley, Jon Antonides, Tim McCormick, Leo Brown, Butch Wade, Gerard Rudy, Leslie Rockymore, Robert McFarland, Ron Gibas

Adirondack stories /

Lost--Lida Ann--John's trial--Joe Baldwin--Willie--Benjamin Jacques--Ike's wife--An Adirondack neighborhood.

Peony and Bamboo by a Rock

Ike no Gyokuran; 3 ft. 39/64 in.x 1 ft. 4 27/64 in.; hanging scroll, ink and color on paper

Dwight David Eisenhower.

Shipping list no.: 1990-0343-P.

University of Michigan Men's Basketball Team, 1980

Back Row: jr. mngr. Robert Murphy, equip. mngr. Bob Hurst, trainer Dan Campbell, sr. mngr. Greg Ruggles, Marty Bodnar, Mike McGee, coach Bill Frieder, John Johnson, Joe James, Mark Bodnar, Dan Pelekoudas, asst. coach Mike Boyd, asst. coach Tom Kempf, grad. asst. Bud VanDeWege Jr.

Front Row: Dean Hopson, Ike Person, John Antonides, co-captain Thad Garner, co-captain Paul Heuerman, Tim McCormick, Leo Brown, Memi. C. Burton

University of Michigan Men's Basketball Team, 1981

Back Row: jr. mngr. Robert Murphy, equip. mngr. Bob Hurst, trainer Dan Campbell, sr. mngr. Greg Ruggles, Marty Bodnar, Mike McGee, coach Bill Frieder, John Johnson, Joe James, Mark Bodnar, Dan Pelekoudas, asst. coach Mike Boyd, asst. coach Tom Kempf, grad. asst. Bud VanDeWege Jr.

Front Row: Dean Hopson, Ike Person, John Antonides, co-captain Thad Garner, co-captain Paul Heuerman, Tim McCormick, Leo Brown, Memi. C. Burton

Psychosocial morbidity in women with abnormal cervical cytology managed by cytological surveillance or initial colposcopy : longitudinal analysis from the TOMBOLA randomised trial

We are grateful for the co-operation and assistance that we received from NHS staff in the co-ordinating centres and clinical sites. We thank the women who participated in TOMBOLA. The TOMBOLA trial was supported by the Medical Research Council (G9700808) and the NHS in England and Scotland. The TOMBOLA Group comprises the following: Grant-holders: University of Aberdeen and NHS Grampian, Aberdeen, Scotland: Maggie Cruickshank, Graeme Murray, David Parkin, Louise Smart, Eric Walker, Norman Waugh (Principal Investigator 2004–2008) University of Nottingham and Nottingham NHS, Nottingham, England: Mark Avis, Claire Chilvers, Katherine Fielding, Rob Hammond, David Jenkins, Jane Johnson, Keith Neal, Ian Russell, Rashmi Seth, Dave Whynes University of Dundee and NHS Tayside, Dundee, Tayside: Ian Duncan, Alistair Robertson (deceased) University of Ottawa, Ottawa, Canada: Julian Little (Principal Investigator 1999–2004) National Cancer Registry, Cork, Ireland: Linda Sharp Bangor University, Bangor, Wales: Ian Russell University of Hull, Hull, England: Leslie G Walker Staff in clinical sites and co-ordinating centres Grampian Breda Anthony, Sarah Bell, Adrienne Bowie, Katrina Brown (deceased), Joe Brown, Kheng Chew, Claire Cochran, Seonaidh Cotton, Jeannie Dean, Kate Dunn, Jane Edwards, David Evans, Julie Fenty, Al Finlayson, Marie Gallagher, Nicola Gray, Maureen Heddle, Alison Innes, Debbie Jobson, Mandy Keillor, Jayne MacGregor, Sheona Mackenzie, Amanda Mackie, Gladys McPherson, Ike Okorocha, Morag Reilly, Joan Rodgers, Alison Thornton, Rachel Yeats Tayside Lindyanne Alexander, Lindsey Buchanan, Susan Henderson, Tine Iterbeke, Susanneke Lucas, Gillian Manderson, Sheila Nicol, Gael Reid, Carol Robinson, Trish Sandilands Nottingham Marg Adrian, Ahmed Al-Sahab, Elaine Bentley, Hazel Brook, Claire Bushby, Rita Cannon, Brenda Cooper, Ruth Dowell, Mark Dunderdale, Dr Gabrawi, Li Guo, Lisa Heideman, Steve Jones, Salli Lawson, Zoë Philips, Christopher Platt, Shakuntala Prabhakaran, John Rippin, Rose Thompson, Elizabeth Williams, Claire Woolley Statistical analysis Seonaidh Cotton, Kirsten Harrild, John Norrie, Linda Sharp External Trial Steering Committee Nicholas Day (chair, 1999–2004), Theresa Marteau (chair 2004-), Mahesh Parmar, Julietta Patnick and Ciaran Woodman.

Effect of reading sport chronicles on the perception of justice and the predisposition to protest actions in soccer

In this study three chronicles from national newspapers (one generalist and two sport press) were analyzed. The chronicles belong to Spain’s soccer final of the King’s Cup in 2014. The aim of the study was to know if there was any influence on the readers’ perception of justice and consequently if this influence could cause a particular predisposition to participate in acts of protest. 462 university students participated. The results showed that different chronicles caused differences in the perception of justice depending on the chronicle read. However, a clear influence on the willingness to participate in acts of protest was not obtained. These results should make us think about the impact of sport press and its influence, and to be aware of the indirect responsibility of every sector on the antisocial behaviors generated by soccer in our country.