Die Chronologie der gaonäischen Epoche vom Beginn des zweiten Jahrtausend der seleucidischen Aera (689 der übl. Zeitr.) bis Saadja

Heinrich Graetz

Die Chronologie der gaonäischen Epoche von Beginn des zweiten Jahrtausend der seleucidischen Aera (689 der übl. Zeitr.) bis Saadja

Heinrich Graetz

[Haggada. 15. Jahrhundert] Bl. 4 a oben [Illustration] : Sitzender Mann, die linke Hand erhebend

Scan von Monochrom-Mikroform

[Haggada] Bl. 21 a [Illustration] : Familie am Sedertisch

Scan von Monochrom-Mikroform

Cissus discolor Bl.

anonym

PDZ Motifs in PTP-BL and RIL Bind to Internal Protein Segments in the LIM Domain Protein RIL

The specificity of protein–protein interactions in cellular signaling cascades is dependent on the sequence and intramolecular location of distinct amino acid motifs. We used the two-hybrid interaction trap to identify proteins that can associate with the PDZ motif-rich segment in the protein tyrosine phosphatase PTP-BL. A specific interaction was found with the Lin-11, Isl-1, Mec-3 (LIM) domain containing protein RIL. More detailed analysis demonstrated that the binding specificity resides in the second and fourth PDZ motif of PTP-BL and the LIM domain in RIL. Immunohistochemistry on various mouse tissues revealed a submembranous colocalization of PTP-BL and RIL in epithelial cells. Remarkably, there is also an N-terminal PDZ motif in RIL itself that can bind to the RIL-LIM domain. We demonstrate here that the RIL-LIM domain can be phosphorylated on tyrosine in vitro and in vivo and can be dephosphorylated in vitro by the PTPase domain of PTP-BL. Our data point to the presence of a double PDZ-binding interface on the RIL-LIM domain and suggest tyrosine phosphorylation as a regulatory mechanism for LIM-PDZ associations in the assembly of multiprotein complexes. These findings are in line with an important role of PDZ-mediated interactions in the shaping and organization of submembranous microenvironments of polarized cells.